scholarly journals Combined use of micro-focused ultrasound and autologous blood cells in correction of involutive skin changes

2019 ◽  
Vol 2 (26) ◽  
pp. 105-109
Author(s):  
Zh. Yu. Yusova ◽  
T. V. Stepanova ◽  
P. A. Belkov
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Focused Ultrasound ◽  
Clinical Effect ◽  
Autologous Blood ◽  
Study Data ◽  
Skin Changes ◽  
Combined Application ◽  
Combined Use ◽  
Qualitative Characteristics

A comparative study of micro-focused ultrasound in monotherapy and combined use with autologous blood cells was conducted. In assessing the effectiveness of the procedures studied the qualitative characteristics of the skin and ultrasound scanning data. All patients (35) with involutional changes of the skin at the procedures were divided into tho groups: combined application of ultrasound macrofocusrange with autologous red blood cells and macrofocusrange ultrasound as monotherapy. The results of the study, data were obtained that showed a more pronounced clinical effect in patients treated with the combined use of ultrasound macrofocusrange with autologous red blood cells.

The Immunosuppressive Aspects of Blood Transfusion

1992 ◽  
Vol 7 (4) ◽  
pp. 176-188 ◽  
Author(s):  
Thomas A. Mickler ◽  
David E. Longnecker
Keyword(s):  
Blood Transfusion ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Allograft ◽  
Autologous Blood ◽  
Immunosuppressive Effect ◽  
Solid Cancer ◽  
Infection Rates ◽  
Allograft Survival ◽  
Packed Red Blood Cells

Blood transfusion is associated with immunosuppression, although the exact etiology of the immunosuppressive effect is not fully understood. The clinical significance of the immunosuppressive effect of blood transfusion has been examined in three situations: (1) studies of renal allograft survival after renal transplantation, (2) outcome studies in patients who have had surgical resection of solid cancer tumors, and (3) studies of infection rates in postoperative patients. In each scenario, the data support the conclusion that transfusion is associated with immunosuppression as manifested by increased renal allograft survival, increased recurrence and mortality rates in patients with cancer, and increased infection rates in postoperative patients who are transfused. Not all studies demonstrate an immunosuppressive effect of transfusion. There are several possible explanations for these discrepancies. First, prognostic variables other than transfusion itself account for the outcome results in these retrospective studies. Second, the extent of immunosuppression may be influenced by the type of blood product transfused, the amount transfused, and the timing of the transfusion; these factors have not been considered in all studies. For example, whole blood has been implicated as having a greater immunosuppressive effect than packed red blood cells, and many studies have shown that more than three units of packed red blood cells are necessary to affect outcome. Controlled animal studies have tested the hypothesis that transfusions increase solid tumor growth or the risk for infection. These studies have yielded conflicting results. Nevertheless, evidence that blood transfusion influences clinical outcome mitigates that a decision to transfuse must consider both risks and benefits of a transfusion; the possible consequences of immunosuppression must be included among the risks. Use of autologous blood, erythropoietin, and, in the future, synthetic hemoglobin may lead to improved outcome in patients with certain disease processes.

scholarly journals Inhibition of the Aquaporin-1 Cation Conductance by Selected Furan Compounds Reduces Red Blood Cell Sickling

2022 ◽  
Vol 12 ◽  
Author(s):  
Pak Hin Chow ◽  
Charles D. Cox ◽  
Jinxin V. Pei ◽  
Nancy Anabaraonye ◽  
Saeed Nourmohammadi ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Water Channel ◽  
Ion Conductance ◽  
Aquaporin 1 ◽  
Hypoxic Conditions ◽  
Combined Application ◽  
Cation Conductance ◽  
Osmotic Water ◽  
Combined Features

In sickle cell disease (SCD), the pathological shift of red blood cells (RBCs) into distorted morphologies under hypoxic conditions follows activation of a cationic leak current (Psickle) and cell dehydration. Prior work showed sickling was reduced by 5-hydroxylmethyl-2-furfural (5-HMF), which stabilized mutant hemoglobin and also blocked the Psickle current in RBCs, though the molecular basis of this 5-HMF-sensitive cation current remained a mystery. Work here is the first to test the hypothesis that Aquaporin-1 (AQP1) cation channels contribute to the monovalent component of Psickle. Human AQP1 channels expressed in Xenopus oocytes were evaluated for sensitivity to 5-HMF and four derivatives known to have differential efficacies in preventing RBC sickling. Ion conductances were measured by two-electrode voltage clamp, and osmotic water permeability by optical swelling assays. Compounds tested were: 5-HMF; 5-PMFC (5-(phenoxymethyl)furan-2-carbaldehyde); 5-CMFC (5-(4-chlorophenoxymethyl)furan-2-carbaldehyde); 5-NMFC (5-(2-nitrophenoxymethyl)-furan-2-carbaldehyde); and VZHE006 (tert-butyl (5-formylfuran-2-yl)methyl carbonate). The most effective anti-sickling agent, 5-PMFC, was the most potent inhibitor of the AQP1 ion conductance (98% block at 100 µM). The order of sensitivity of the AQP1 conductance to inhibition was 5-PMFC > VZHE006 > 5-CMFC ≥ 5-NMFC, which corresponded with effectiveness in protecting RBCs from sickling. None of the compounds altered AQP1 water channel activity. Combined application of a selective AQP1 ion channel blocker AqB011 (80 µM) with a selective hemoglobin modifying agent 5-NMFC (2.5 mM) increased anti-sickling effectiveness in red blood cells from human SCD patients. Another non-selective cation channel known to be expressed in RBCs, Piezo1, was unaffected by 2 mM 5-HMF. Results suggest that inhibition of AQP1 ion channels and capacity to modify hemoglobin are combined features of the most effective anti-sickling agents. Future therapeutics aimed at both targets could hold promise for improved treatments for SCD.

scholarly journals Selective ablative laser technologies in the correction of involutional skin changes

2021 ◽  
Vol 18 (4) ◽  
pp. 248-251
Author(s):  
Zhanna Yu. Yusova ◽  
Elena L. Baranova
Keyword(s):  
Clinical Effect ◽  
Combined Exposure ◽  
Skin Changes ◽  
Combined Application ◽  
Laser Technologies ◽  
Degree Of Satisfaction ◽  
Ultrasonic Parameters ◽  
Group 2 ◽  
Ablative Laser ◽  
Group 1

Using a combined application of surface and deep fractional ablation allows you to solve a wider range of problems in the correction of aesthetic deficiencies in the case of involute skin changes. The study examined the clinical efficacy of the interference of the superficial and deep fractional ablation on epiflourescent and ultrasonic parameters of the skin. There were 52 patients under observation, who were divided into 2 groups according to the treatment regimen. Surface fractional ablation was performed in group 1, and interference of surface and deep fractional ablation was performed in group 2. The analysis of clinical data and the degree of satisfaction with the procedures showed a more pronounced clinical effect when performing combined exposure.

scholarly journals Effects of remifentanil and propofol on distant organ lung injury in an ischemia–reperfusion model

Open Medicine ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. 1673-1680
Author(s):  
Orhan Kanbak ◽  
Burcu Aydoğan ◽  
Tülin Gümüş
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Lung Injury ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Ischemia Reperfusion ◽  
Lipid Peroxidation Product ◽  
Tissue Samples ◽  
Combined Use ◽  
Distant Organ

Abstract Our aim was to evaluate lung injury due to oxidative stress and antioxidant activity levels in an infrarenal ischemia–reperfusion model and to compare prevention effects of single and combined use of propofol and remifentanil. In this study, a total of 40 adult Wistar Albino rats were randomly divided into five groups of eight rats as SHAM, physiological saline, intraperitoneal propofol, remifentanil, and propofol and remifentanil groups. Blood and tissue samples were obtained after 80 min of reperfusion. The malondialdehyde (MDA) level, a measure of lipid peroxidation, was measured in lung tissue samples and red blood cells; additionally, total oxidant status and total antioxidant capacity of lung tissues were measured and histopathological examination was performed. Distant organ (lung) injury developed due to lower extremity ischemia–reperfusion was created by infrarenal aortic clamping. The lipid peroxidation product MDA and total oxidant levels were increased, but there was insufficient antioxidant protection both in the lung tissues and red blood cells. While propofol prevented this injury consistent with its proposed antioxidant properties; no protective effect of remifentanil was observed. On the contrary, it showed oxidative stress increasing effect. This study concluded that the antioxidant effect of propofol was suppressed by remifentanil in the case of combined use.

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