Effects of in ovo injection of prebiotics and synbiotics on the productive performance and microstructural features of the superficial pectoral muscle in broiler chickens

To better understand the effects of synbiotics administered at early stages of embryonic development in poultry, it is necessary to analyze direct effects (meat quality) and the molecular background. The molecular interpretation of poultry meat properties after in ovo administration of synbiotics remains to be reported. The purpose of the present study was to analyze the molecular background of meat quality based on gene expression and basic physiological parameters. Eggs were injected with (S1) Lactobacillus salivarius with galacto-oligosaccharides or (S2) Lactobacillus plantarum with raffinose family oligosaccharides. The pectoral muscle was collected at two time points (day 7 and day 42) and subjected to RNA isolation. Gene expression analysis was performed by RT-qPCR for a panel of eight genes associated with metabolism. The concentration of glucose and hormones (insulin, glucagon, and leptin (S1 p = 0.04)) was also increased. The obtained results showed that metabolic gene expression in the muscle was more differential due to synbiotic stimulation on day 7 (FST in S1 p = 0.03; PDK4 in S1 p = 0.02 and S2 p = 0.01; CEBPB in S1 p = 0.01 and S2 p = 0.008; PHKB in S1 p = 0.01; PRKAG3 in S1 p = 0.02) than on day 42 (PDK4 in S1 p = 0.04). On the basis of the results obtained, it can be concluded that in ovo stimulation with S1 triggered the most potent and favorable changes in the pectoral muscle gene expression in broiler chickens.

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INFLUENCE OF IN OVO INOCULATION OF BIFIDOBACTERIA ON PRODUCTIVE PERFORMANCE, ANTIOXIDANT AND IMMUNE STATUS AND GUT MICROFLORAOF BROILER CHICKENS

Egyptian Journal of Nutrition and Feeds ◽

10.21608/ejnf.2017.75184 ◽

2017 ◽

Vol 20 (2) ◽

pp. 267-276

Author(s):

F. Badri ◽

Shimaa Amin

Keyword(s):

Broiler Chickens ◽

Immune Status ◽

Productive Performance ◽

In Ovo

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EFFECT OF IN-OVO INJECTION WITH IRON–METHIONINE CHELATES OR IRON NANO-PARTICLES AND POST HATCH DIETARY FOLIC ACID ON GROWTH PERFORMANCE AND PHYSIOLOGICAL RESPONSES OF BROILER CHICKENS

Egyptian Poultry Science Journal ◽

10.21608/epsj.2019.63487 ◽

2019 ◽

Vol 39 (4) ◽

pp. 753-770

Author(s):

M. El-Gogary ◽

E. El-Said

Keyword(s):

Folic Acid ◽

Growth Performance ◽

Broiler Chickens ◽

Physiological Responses ◽

Nano Particles ◽

In Ovo ◽

In Ovo Injection

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Effects of Probiotics, Prebiotics and Synbiotics Injected in Ovo on the Microstructure of the Breast Muscle in Different Chicken Genotypes

Animals ◽

10.3390/ani11102944 ◽

2021 ◽

Vol 11 (10) ◽

pp. 2944

Author(s):

Karolina Stasiak ◽

Anna Slawinska ◽

Joanna Bogucka

Keyword(s):

Broiler Chickens ◽

Fibre Diameter ◽

Pectoral Muscle ◽

General Purpose ◽

Muscle Fibres ◽

Histopathological Changes ◽

In Ovo ◽

Fibre Density ◽

Genotype Group ◽

Bioactive Substance

The aim of the study was to analyse the effect of probiotics, prebiotics and synbiotics injected in ovo on day 12 of embryonic development on the microstructure of the superficial pectoral muscle (musculus pectoralis superficialis) from 42-day-old chickens of different genotypes: broilers (Ross 308) and general-purpose type (green-legged partridge (GP) chickens Zk-11, native chickens). Incubated eggs were divided into four groups (each genotype separately) depending on the substance injected in ovo: normal saline (C, control); Lactococcus lactis subsp. cremoris (PRO); galactooligosaccharides, GOS (PRE) or GOS + L. lactis (SYN). After hatching, chicks were placed in eight replicated pens (four pens/genotype group). There were eight birds per pen. In total, 64 birds were used in the experiment. Birds were slaughtered at the age of 42 days, and samples of superficial pectoral muscles were taken for analysis. The microstructure of the pectoral muscles was evaluated using the cryosectioning (frozen tissue sectioning) technique and staining with haematoxylin and eosin. Statistical analysis revealed that the in ovo injection of probiotics, prebiotics and synbiotics had no significant effect on the diameter of muscle fibres from chickens of the two genotypes. The number of fibres in the muscles from green-legged partridge chickens was about three-fold higher than the fibre density in the muscles from broiler chickens, with the fibre diameter being two-fold smaller. This fact may indicate a greater tenderness of meat from GP chickens compared to the meat from Ross 308 broilers. In the case of broilers, a prebiotic (GOS) was the most effective bioactive substance in reducing the number of histopathological changes. Considering muscles from GP chickens, the number of normal fibres was highest in birds treated with the probiotic. These findings indicate that the microstructural features of pectoral muscles depend not only on the type of the injected bioactive substance but also on the genotype of chickens.

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