scholarly journals Identification of Additive–Epistatic QTLs Conferring Seed Traits in Soybean Using Recombinant Inbred Lines

2020 ◽  
Vol 11 ◽  
Author(s):  
Meng Li ◽  
Lingling Chen ◽  
Jian Zeng ◽  
Muhammad Khuram Razzaq ◽  
Xianchao Xu ◽  
...  

Seed weight and shape are important agronomic traits that affect soybean quality and yield. In the present study, we used image analysis software to evaluate 100-seed weight and seed shape traits (length, width, perimeter, projection area, length/width, and weight/projection area) of 155 novel recombinant inbred soybean lines (NJRISX) generated by crossing “Su88-M21” and “XYXHD”. We examined quantitative trait loci (QTLs) associated with the six traits (except seed weight per projection area), and identified 42 additive QTLs (5–8 QTLs per trait) accounting for 24.9–37.5% of the phenotypic variation (PV). Meanwhile, 2–4 epistatic QTL pairs per trait out of a total of 18 accounted for 2.5–7.2% of the PV; and unmapped minor QTLs accounted for the remaining 35.0–56.7% of the PV. A total of 28 additive and 11 epistatic QTL pairs were concentrated in nine joint QTL segments (JQSs), indicating that QTLs associated with seed weight and shape are closely related and interacted. An interaction was also detected between additive and epistatic QTL pairs and environment, which made significant contributions of 1.4–9.5% and 0.4–0.8% to the PV, respectively. We annotated 18 candidate genes in the nine JQSs, which were important for interpreting the close relationships among the six traits. These findings indicate that examining the interactions between closely related traits rather than only analyzing individual trait provides more useful insight into the genetic system of the interrelated traits for which there has been limited QTL information.

2018 ◽  
Vol 16 (5) ◽  
pp. 424-436 ◽  
Author(s):  
Carol Moreau ◽  
Maggie Knox ◽  
Lynda Turner ◽  
Tracey Rayner ◽  
Jane Thomas ◽  
...  

AbstractIn order to gain an understanding of the genetic basis of traits of interest to breeders, the pea varieties Brutus, Enigma and Kahuna were selected, based on measures of their phenotypic and genotypic differences, for the construction of recombinant inbred populations. Reciprocal crosses were carried out for each of the three pairs, and over 200 F2 seeds from each cross advanced to F13. Bulked F7 seeds were used to generate F8–F11 bulks, which were grown in triplicated plots within randomized field trials and used to collect phenotypic data, including seed weight and yield traits, over a number of growing seasons. Genetic maps were constructed from the F6 generation to support the analysis of qualitative and quantitative traits and have led to the identification of four major genetic loci involved in seed weight determination and at least one major locus responsible for variation in yield. Three of the seed weight loci, at least one of which has not been described previously, were associated with the marrowfat seed phenotype. For some of the loci identified, candidate genes have been identified. The F13 single seed descent lines are available as a germplasm resource for the legume and pulse crop communities.


2011 ◽  
Vol 5 (2) ◽  
pp. 135-146 ◽  
Author(s):  
P. Haddadi ◽  
B. Yazdi-samadi ◽  
M. R. Naghavi ◽  
A. Kalantari ◽  
P. Maury ◽  
...  

2018 ◽  
Vol 68 (5) ◽  
pp. 587-595 ◽  
Author(s):  
Yuki Sakai ◽  
Liangzi Cao ◽  
Ryosuke Funata ◽  
Takatou Shiraishi ◽  
Koki Yoshikawa ◽  
...  

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