Influence of Environmental Temperature and Hormonal Stimulation on the In Vitro Sperm Maturation in Sterlet Acipenser ruthenus in Advance of the Spawning Season

Sturgeon sperm maturation occurs outside the testes during the transit of testicular spermatozoa (TS) through the kidneys and the Wolffian ducts. A method of in vitro TS maturation in sterlet Acipenser ruthenus was used to investigate the effects of temperature and hormonal stimulation of spermiation on the ability of TS to complete this process. Spermatozoa motility parameters after in vitro maturation of testicular sperm, concentrations of sex steroid hormones and testis morphology were studied in three groups of sterlet: (1) after overwintering in ponds (OW), (2) adapted to spawning temperature (ST), and (3) adapted to spawning temperature with hormonal induction of spermiation (ST-HI). Blood plasma concentrations of testosterone, 11-ketotestosterone and 17,20β-dihydroxy-pregnenolone increased significantly after hormonal induction of spermiation (group ST-HI). In all groups, TS were not motile. After in vitro sperm maturation, motility was up to 60% only in group ST-HI. The data suggest that the ability of TS to be matured in vitro was not related to the environmental temperature, while hormonal stimulation of spermiation during the spawning season was an absolute requirement for optimal in vitro maturation.

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In vitro sperm maturation in sterlet, Acipenser ruthenus

Reproductive Biology ◽

10.1016/j.repbio.2014.01.003 ◽

2014 ◽

Vol 14 (2) ◽

pp. 160-163 ◽

Cited By ~ 30

Author(s):

Borys Dzyuba ◽

Jacky Cosson ◽

Sergii Boryshpolets ◽

Olga Bondarenko ◽

Viktoriya Dzyuba ◽

...

Keyword(s):

Sperm Maturation ◽

Acipenser Ruthenus ◽

Sterlet Acipenser Ruthenus

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Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus

Reproduction Fertility and Development ◽

10.1071/rd16145 ◽

2017 ◽

Vol 29 (7) ◽

pp. 1319 ◽

Cited By ~ 4

Author(s):

Olga Bondarenko ◽

Borys Dzyuba ◽

Marek Rodina ◽

Jacky Cosson

Keyword(s):

Sperm Motility ◽

Seminal Fluid ◽

Mature Male ◽

Sperm Maturation ◽

Wolffian Duct ◽

Testicular Spermatozoa ◽

Acipenser Ruthenus ◽

Sterlet Acipenser Ruthenus ◽

Motility Parameters

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.

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In vitro hormonal stimulation of [14C]acetate incorporation by Heliothis armigera pheromone glands

Insect Biochemistry ◽

10.1016/0020-1790(89)90002-4 ◽

1989 ◽

Vol 19 (1) ◽

pp. 1-5 ◽

Cited By ~ 73

Author(s):

V. Soroker ◽

A. Rafaeli

Keyword(s):

Heliothis Armigera ◽

Acetate Incorporation ◽

Hormonal Stimulation ◽

Pheromone Glands ◽

Stimulation Of

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P–442 Cancer does not adversely affect oocyte maturation in vitro with the exception of breast cancer

Human Reproduction ◽

10.1093/humrep/deab130.441 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

I Viran. . Klun ◽

J Bedenk ◽

N Jancar

Keyword(s):

Breast Cancer ◽

Cancer Patients ◽

Oocyte Maturation ◽

In Vitro Maturation ◽

Hormonal Stimulation ◽

Infertile Women ◽

Maturation In Vitro ◽

Infertile Patients

Abstract Study question Do different types of cancer affect the success of oocyte maturation in vitro compared to infertile women included in the in vitro fertilization (IVF) program? Summary answer Cancer does not adversely affect oocyte maturation in vitro, with the exception of breast cancer, compared to infertile women in the in vitro fertilization program. What is known already Vitrification and storage of oocytes in liquid nitrogen is one of the real options for maintaining reproductive function in cancer patients. Despite careful hormonal stimulation of the ovaries, however, the proportion of oocytes is immature and lost to the patient. In vitro maturation of oocytes can play an important role in resolving immature oocytes and increasing the chances of conception in cancer patients. Moreover, it can mean a safe way to store oocytes when ovarian hormonal stimulation could worsen the disease. Therefore, the aim of this study was to determine whether different types of cancer affect oocyte in vitro maturation. Study design, size, duration After ovarian stimulation in 18 cancer patients, the number and maturity of oocytes were compared to 21 infertile patients in the IVF program over a three-year period. In both groups, 119 germinal vesicle-GV oocytes were matured in vitro to compare the maturation rate. After IVF in a subset of 17 infertile patients, the fertilization of in vitro and in vivo matured oocytes was compared in the same cycles. The procedure was considered in cancer patients. Participants/materials, setting, methods In this prospective study, forty-five GV oocytes in cancer patients and 74 GV oocytes in infertile patients underwent in vitro maturation procedure. Each oocyte was matured in vitro in the MediCult IVM System by conditioning in LAG medium and maturation for up to 28 hours in IVM medium with added hormones FSH and hCG, in coculture with cumulus cells from mature oocytes in the same patients. Oocytes were fertilized by intracytoplasmic sperm injection (ICSI). Main results and the role of chance After controlled ovarian hormonal stimulation, 198 oocytes were retrieved in cancer patients and 259 oocytes in infertile women and there were no significant differences in the number of retrieved oocytes, proportion of degenerated oocytes and proportion of GV oocytes. In cancer patients, the proportion of oocytes that matured in vitro was lower than in infertile patients (66.0 vs. 80.0%), but the difference was not significant. Among cancer patients, the oocyte maturation rate tended to be lower in patients with breast cancer than in patients with other cancers (54.5% vs. 81.2%; difference not significant). However, in patients with breast cancer, significantly fewer oocytes matured in vitro than in infertile patients (54.5% vs. 80.0%; P < 0.05, Chi-Square test) even though they tended to be younger (29.3 ± 7.4 vs. 33.4 ± 5.0 years; non-significant difference). After in vitro maturation, there was a 13% increase in mature oocyte yield in cancer patients and a 20.1% increase in infertile women with no significant difference observed. After ICSI in a subset of infertile women, there was approximately the same fertilization rate between oocytes matured in vitro and in vivo (55.1% vs. 57.0%) in the same cycles. Limitations, reasons for caution For ICSI in oocytes matured in vitro, we had to use semen collected the day before, while oocytes matured in vivo were fertilized with fresh semen in the same cycle. Therefore, we could not compare the development of embryos in both groups. Wider implications of the findings: In vitro maturation of oocytes in connection with their vitrification or vitrification of embryos after their fertilization appears to be a valuable way to maintain the fertility of young cancer patients, but a worse outcome is expected in breast cancer patients. Trial registration number National Medical Ethical Committee Approval, No. 0120–222/2016–2; KME 115/04/16.

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L-OPU in Goat and Sheep—Different Variants of the Oocyte Recovery Method

Animals ◽

10.3390/ani10040658 ◽

2020 ◽

Vol 10 (4) ◽

pp. 658

Author(s):

Jarosław Wieczorek ◽

Jurij Koseniuk ◽

Maria Skrzyszowska ◽

Mirosław Cegła

Keyword(s):

In Vitro Maturation ◽

Hormonal Stimulation ◽

Recovery Method ◽

Vitro Fertilization ◽

Minimal Invasiveness ◽

Animal Reproduction ◽

Oocyte Recovery ◽

Maturation In Vitro ◽

Invasive Techniques

The laparoscopic method of recovering oocytes in goats and sheep is one of the minimally invasive methods used in the biotechnology of animal reproduction. It allows for good quality oocytes that are suitable for in vitro maturation and fertilization to be recovered. The limitation of using the laparoscopic ovum pick-up (L-OPU) method in goat and sheep is its changing effectiveness and the lack of repeatability of results, as well as the varying effectiveness of different variants of the method. Therefore, it is necessary to develop effective non-invasive techniques allowing for multiple good quality oocyte recovery that would be suitable for in vitro maturation and fertilization. In this study, four different L-OPU variants were described in goats and sheep. Various techniques of recovering oocytes were discussed, including the techniques of conducting the operation, various tools for recovering oocytes, and different plans of hormonal stimulation. Recovery rates were 35% (Variant I), 57% (Variant II), 72% (Variant III), and 67% (Variant IV). After evaluation, 94% (both Variant I and II), 93% (Variant III), and 84% (Variant IV) of the oocytes were qualified for in vitro maturation. The results of the study show that the proposed technique of laparoscopic recovery of oocytes allows a sufficient number of ovarian cells suitable for in vitro culture to be obtained and as a consequence it makes them useful in in vitro maturation/in vitro fertilization (IVM/IVF) programs or cloning. The method allows for a fast and effective conduct of the operation in a living donor with minimal invasiveness while preserving the excellent condition of animals.

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In vitro effects of bisphenol A on the quality parameters, oxidative stress, DNA integrity and adenosine triphosphate content in sterlet (Acipenser ruthenus) spermatozoa

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology ◽

10.1016/j.cbpc.2013.05.002 ◽

2013 ◽

Vol 158 (2) ◽

pp. 64-71 ◽

Cited By ~ 47

Author(s):

Martin Hulak ◽

Ievgeniia Gazo ◽

Anna Shaliutina ◽

Pavla Linhartova

Keyword(s):

Oxidative Stress ◽

Bisphenol A ◽

Adenosine Triphosphate ◽

Quality Parameters ◽

Dna Integrity ◽

Acipenser Ruthenus ◽

In Vitro Effects ◽

Sterlet Acipenser Ruthenus

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PRACTICE OF ARTIFICIAL REPRODUCTION OF STERLAD (ACIPENSER RUTHENUS LINNAEUS) IN RECIRCULATING AQUACULTURE SYSTEM (IN MANGISTAU REGION)

VESTNIK OF ASTRAKHAN STATE TECHNICAL UNIVERSITY SERIES FISHING INDUSTRY ◽

10.24143/2073-5529-2018-3-111-124 ◽

2020 ◽

Vol 2020 (3) ◽

pp. 111-124

Author(s):

Yefim Fedorovich Bulavin ◽

Guldana Maratkizi Maratova ◽

Saule Zhangirovna Asylbekova ◽

Kuanysh Baibulatovich Isbekov ◽

Saya Kashkinbayevna Koyshibaeva ◽

...

Keyword(s):

Fish Farming ◽

Artificial Reproduction ◽

Recirculating Aquaculture System ◽

Hormonal Stimulation ◽

Commercial Fish ◽

Acipenser Ruthenus ◽

Recirculating Aquaculture ◽

Aquaculture System ◽

Sturgeon Species ◽

Sterlet Acipenser Ruthenus

The paper presents the results of artificial reproduction of sterlet (Acipenser ruthenus Linnaeus) carried out at the Scientific Production Center KazakhOsseter, LLP (the Mangistau region) in conditions of a recirculating aquatic system (RAS). The conditions for carrying out the main reproduction measures are described: wintering, qualifying, hormonal stimulation, obtaining reproductive products and rearing larvae. As a result, the sterlet spawning campaign was carried out in conditions of RAS in the Mangistau region, the native grown viable sterlet larvae being obtained. In the course of the work carried out in conditions of RAS in the Mangistau region there have been developed the scientific foundations and technologies for the formation and using production stocks of sturgeon, which are involved in the process of artificial reproduction. The results of the work performed could help to effectively organize the production at the commercial fish farming enterprises in the Mangistau region. The entrepreneurs of the region will have the opportunity to get acquainted with the technology of fish growing in conditions of RAS and to apply the technology for obtaining and reproducing sturgeon species in the ir local hatcheries in the future.

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