Haematology and Serum Biochemical Indices of Lambs Supplemented with Moringa oleifera, Jatropha curcas and Aloe vera Leaf Extract as Anti-Methanogenic Additives

Medicinal plants have been found to be effective in a wide range of applications in ruminant animals. However, some plant extracts may be toxic to animals, depending on their seconday metabolite composition and dose, and therefore, animal trials are needed to validate their safety when used as anti-methanogenic additives. This study investigated the effect of three plant extracts used as anti-methanogenic dietary additives, on the haematology and serum biochemical parameters in sheep. Methanolic extracts of Moringa oleifera (MO), Jatropha curcas (JC) and Aloe vera (AV) were orally dosed as experimental treatments for 75 days to sheep, and their effect on the haematology and serum biochemical parameters of SA Mutton Merino (SAMM) lambs were compared with sheep on a control treatment without any additive treatment. Extracts of MO, JC and AV were extracted in 100% methanol, freeze-dried, and reconstituted in distilled water. A total of 40 lambs were ranked according to their body weight into a group of four and one sheep at a time was randomly allocated into four dietary treatments which include a control treatment, and treatment with either MO, JC or AV extract. Lambs were drenched twice daily with doses equivalent to 50 mg/kg dry matter intake (DMI) based on previous week feed consumption. Blood samples were collected via jugular vein puncture and analysed for haematology and serum biochemistry parameters, using standard procedures. The results of the haematological analysis showed that most haematological parameters were not affected by plant extract used as anti-methanogenic additives (p > 0.05), except for higher white blood cell (WBC) and lymphocytes counts recorded in control lambs and lambs in the AV treatment. All serum biochemical properties (except alkaline phosphatase) were not different (p > 0.05) between the control and lambs treated with plant extracts. Alkaline phosphatase was influenced by the plant extract (p < 0.05), with lambs receiving MO, JC and AV having lower alkaline phosphatase concentrations compared to lambs on the control diet without any additive. The result of the study showed that extracts of MO, JC and AV were not toxic to sheep when used as antimethanogenic additives at the recommended dose of 50 mg/kg dry matter feed which had proved previously to be effective in reducing enteric methane emission. Therefore, these plant extracts could be used safely as alternative dietary additives to reduce enteric methane emission and boost the productivity of SA Mutton Merino sheep.

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Effect of methanol extract of Moringa oleifera Lam (Moringaceceae) Young Pods on some Serum biochemical parameters in wistar rats

Advance Pharmaceutical Journal ◽

10.31024/apj.2018.3.1.3 ◽

2018 ◽

Vol 3 (1) ◽

Keyword(s):

Biochemical Parameters ◽

Wistar Rats ◽

Moringa Oleifera ◽

Methanol Extract ◽

Serum Biochemical Parameters ◽

Serum Biochemical ◽

Moringa Oleifera Lam

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Effects of Probiotics as Antibiotics Substitutes on Growth Performance, Serum Biochemical Parameters, Intestinal Morphology, and Barrier Function of Broilers

Animals ◽

10.3390/ani9110985 ◽

2019 ◽

Vol 9 (11) ◽

pp. 985 ◽

Cited By ~ 4

Author(s):

Tengfei He ◽

Shenfei Long ◽

Shad Mahfuz ◽

Di Wu ◽

Xi Wang ◽

...

Keyword(s):

Organic Matter ◽

Growth Performance ◽

Biochemical Parameters ◽

Dry Matter ◽

Phase 1 ◽

Basal Diet ◽

Intestinal Morphology ◽

Serum Biochemical Parameters ◽

Phase 2 ◽

Serum Biochemical

The aim of this study was to investigate the effects of the combination of probiotics replacing antibiotics on growth performance, serum biochemical parameters, intestinal morphology, and expression of tight junction proteins in intestinal mucosa of broilers. A total of 168 Arbor Acres broilers (45.04 ± 0.92 g) were randomly divided into three treatments, with seven replicates per treatment, and eight broilers per replicate. The experiment included phases 1 (d 0 to 21) and 2 (d 21 to 42). The dietary treatments contained a corn soybean meal-based diet (control group; CON); an antibiotic group (basal diet + 75 mg/kg chlortetracycline; CTC), and a probiotics group (basal diet + probiotics (500 mg/kg in phase 1 and 300 mg/kg in phase 2; Bacillus subtilis 5 × 109 CFU/g, Bacillus licheniformis 2.5 × 1010 CFU/g and Saccharomyces cerevisiae 1 × 109 CFU/g; PB). The results showed broilers fed PB had improved (p < 0.05) feed conversion ratio (FCR) in phase 1 and increased (p < 0.05) average daily gain (ADG) in phase 2, as well as improved (p < 0.05) ADG and FCR overall (d 0 to 42). The apparent total tract digestibility (ATTD) of dry matter, organic matter, gross energy, and crude protein was increased (p < 0.05) in broilers fed PB, while the ATTD of dry matter and organic matter was enhanced in broilers fed CTC compared with CON. Broilers fed PB showed increased (p < 0.05) serum total antioxidant capacity concentrations and tended to have higher (p = 0.06) level of serum immunoglobulin M in phase 1 compared with CON. These broilers also had increased (p < 0.05) level of serum immunoglobulin A in phase 2 in comparison with CON and CTC. Moreover, broilers fed CTC and PB showed increased (p = 0.05) villus height to crypt depth ratio in duodenum, as well as higher (p < 0.05) mRNA expression of zonula occludens-1 in jejunum compared with CON. In conclusion, dietary supplementation with PB as chlortetracycline substitute could improve the growth performance, nutrient digestibility, serum antioxidant capacity, jejunal mucosal barrier function, and intestinal morphology of broilers.

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Effects of Moringa oleifera and Garcinia kola with or without Grits on Haematological and Serum Biochemical Parameters of Broiler Chickens.

Journal of World's Poultry Research ◽

10.36380/jwpr.2019.22 ◽

2019 ◽

Vol 9 (4) ◽

pp. 180-186

Author(s):

YA Adejola ◽

R.A Sobayo ◽

SB Muhammad ◽

AA Ayoola ◽

KB Jinadu

Keyword(s):

Biochemical Parameters ◽

Broiler Chickens ◽

Moringa Oleifera ◽

Serum Biochemical Parameters ◽

Garcinia Kola ◽

Serum Biochemical

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Dietary Supplementation of Lysophospholipids Affects Feed Digestion in Lambs

Animals ◽

10.3390/ani9100805 ◽

2019 ◽

Vol 9 (10) ◽

pp. 805 ◽

Cited By ~ 1

Author(s):

Qin Huo ◽

Bo Li ◽

Long Cheng ◽

Tingting Wu ◽

Peihua You ◽

...

Keyword(s):

Bacterial Community ◽

Biochemical Parameters ◽

Dry Matter ◽

Control Diet ◽

Average Daily Gain ◽

Live Weight ◽

Nutrient Digestibility ◽

Neutral Detergent Fiber ◽

Serum Biochemical Parameters ◽

Serum Biochemical

Five experiments were conducted to examine effects of lysophospholipids (LPL) on live weight gain, nutrient digestibility, ruminal fermentation parameters, serum biochemical parameters and rumen bacterial community profile in fattening lambs. Two dietary treatments (pelleted complete feed supplemented without (control diet; CON) or with 0.05% LPL on dry matter basis) were tested in these experiments. Feed and water were provided ad libitum to lambs. The results showed that average daily gain (ADG) tended to increase or was not affected by LPL supplementation. Compared with CON, the supplementation of LPL resulted in an increase in dry matter, crude protein and organic matter digestibilities, and a decrease in neutral detergent fiber and acid detergent fiber digestibilities. Ruminal pH values did not change with LPL supplementation, but the concentrations of ammonia and total short chain fatty acids (SCFAs) were increased. The molar proportion of major individual SCFAs and the ratio of acetate to propionate were not affected by LPL supplementation. While the activity of lipase was decreased with LPL supplementation, all other serum biochemical parameters did not change. Rumen bacterial community was altered by LPL supplementation with the relative abundance of fibrolytic bacteria in the total bacterial population, such as Prevotella, decreased. In conclusion, LPL supplementation can alter feed digestion, but may not result in consistent positive responses in animal growth performance.

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Influence of Irradiated Moringa oleifera Leaf Meal as Dietary Supplement on some Serum Biochemical Parameters of Growing Rabbits

Arab Journal of Nuclear Sciences and Applications ◽

10.21608/ajnsa.2021.88375.1495 ◽

2021 ◽

Vol 0 (0) ◽

pp. 1-11

Author(s):

Aly Eissa ◽

Safaa AbdElWahab ◽

Amal Mansour ◽

Usama Abdel Monem

Keyword(s):

Dietary Supplement ◽

Biochemical Parameters ◽

Moringa Oleifera ◽

Serum Biochemical Parameters ◽

Leaf Meal ◽

Serum Biochemical

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Efficacy of Ginger and Nutritox® in counteracting aflatoxin effects on white Pekin ducklings

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2019.20.404 ◽

2019 ◽

Vol 20 (4) ◽

pp. 21-28

Author(s):

Walaa Abu El-Ela ◽

Kamel IAbou.Elazm ◽

Sanaa Awad

Keyword(s):

Experimental Study ◽

Alkaline Phosphatase ◽

Adverse Effects ◽

Biochemical Parameters ◽

Basal Diet ◽

Control Group ◽

Serum Biochemical Parameters ◽

Commercial Broiler ◽

Serum Biochemical ◽

Liver Changes

Objective: To evaluate the anti-aflatoxin effects of Ginger and Nutritox® in white Pekin ducklings. Design: Randomized experimental study Animals: Ninty- one-day-old white Pekin ducklings. Procedure: Ducklings were alienated randomly into six equal groups, each group in 3 replicates (each replicate, n=5). Control group G1) was fed on commercial broiler basal diet without any medications, while other experimental groups, namely 2, 3, 4, 5 and 6 were received diet containing: G2) basal diet (BD) + 300 ppb AF (aflatoxin); G3) BD + 300 ppb AF + 250 mg kg-1 Gr (Ginger); G4) BD +300 ppb AF +1gm/kg feed Nutritox®; G5) BD + 250 mg kg-1 mg/kg of B.W Gr; G6) BD + 1gm/kg feed Nutritox® during study (1-14) days respectively. Results: Results showed that, addition of Ginger and Nutritox® ameliorated in different degrees the adverse effects of aflatoxin and improved growth performance and some serum biochemical parameters [Aspartate aminotransferase (AST), alanine aminotransferase (ALT) and Alkaline phosphatase (ALP)]. They hasten the recovery of antioxidant enzymes (MDA and GSH) in tissue to their normal levels during certain periods of our experiments. Histopathological liver changes run in the same track with biochemical results. Results revealed that administration of Ginger or Nutritox® in diet prevents and/or reduce some adverse effects of aflatoxin in ducks. Conclusion and clinical relevance: Our study indicates that ginger, an alternative natural product can provide protection against aflatoxin as Nutritox®.

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