scholarly journals Extra Virgin Olive Oil Protects the Testis and Blood from the Toxicity of Paracetamol (Overdose) in Adult Male Rats

Biology ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1042
Author(s):  
Latifa Ishaq Khayyat
Keyword(s):  
Olive Oil ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Paracetamol Overdose ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Ultrastructural Studies ◽  
Group 4 ◽  
Group 3

Extra virgin olive oil (EVOO) is important in people’s daily diets. Paracetamol is a widely used analgesic and antipyretic drug. The aim of this study is to investigate the protective effect of EVOO against hematotoxicity and testicular toxicity induced by paracetamol overdose in rats. Forty rats were divided into four groups. Group 1 rats were given water (control), Group 2 rats were given oral EVOO daily (2 mL/kg b.wt.), Group 3 rats were given oral paracetamol daily (650 mg/kg b.wt.), and Group 4 rats were given paracetamol and EVOO daily. After 15 days, blood and testis samples were collected for biochemical, histological, and ultrastructural studies. The results show that paracetamol decreased the PCV, Hb, and RBC counts relative to the control, and significantly increased the WBC counts and stab cells in Group 3. A significant decrease in blood testosterone was found in Group 3 compared to the control, while a significant increase in testosterone levels was observed in Group 4 compared to Group 3. Light and electron microscopy showed disorganized seminiferous tubules in Group 3. The testis in Group 4 appeared in normal shape. In conclusion, the results indicate that EVOO protects the testis and blood from paracetamol toxicity and may also increase fertility in male rats.

scholarly journals The Enhanced Beneficial Effect of Extra Virgin Olive Oil Over Evening Primrose Oil on Oxidative Stress, and Liver Function in Male Arthritic Rats

2021 ◽  
pp. 14-26
Author(s):  
Mohamed A. Kandeil ◽  
Sana’a O. Ebrahim ◽  
Basant M. Mahmoud
Keyword(s):  
Rheumatoid Arthritis ◽  
Oxidative Stress ◽  
Olive Oil ◽  
Liver Enzymes ◽  
Virgin Olive Oil ◽  
Antioxidant Properties ◽  
Extra Virgin Olive Oil ◽  
Male Rats ◽  
Evening Primrose Oil ◽  
Evening Primrose

Aims: Rheumatoid arthritis (RA) is characterized by the onset of oxidative stress. This study aimed to evaluate the enhancing of extra virgin olive (EVOO) and Evening primrose oil (EPO) on oxidative stress and liver enzymes in male Wistar rats and compare between them. Place and Duration: Faculty of Science biochemistry department, Between July 2018 and August 2018. Methodology: A Subcutaneous injection of 200 µl of Freund's complete adjuvant into a footpad of the right hind leg of Wistar male rats at two consecutive days induced RA. Rats received EVOO and EPO daily by oral gavage needle with gauge 18 at doses of 5 mg/kg b.wt./day. for 10 and 21 days. No loss was recorded in the experimental rats. Results: A significant depletion in serum Reduced glutathione content (GSH), glutathione peroxidase (GPX), and glutathione s transferase activities (GST) in arthritic rats compared to normal rats after 10 and 21 days of induction which improved significantly after 10 and 21 days of EPO and EVOO treatments. EPO and EVOO treatments for 21 days increased the GSH and GPX compared to 10 days treatments while no difference in GST activity. EVOO treatment improved GSH and GPX after 10 and 21 days than EPO treatment. The elevated uric acid levels in arthritic rats were markedly ameliorated as a result of EVOO and EPO treatment administration. Increased lipid peroxidation products (MDA), rheumatoid factor, and liver enzyme (Alanine transaminase ALT and Aspartate transaminase AST) were recorded in arthritic rats and they significantly progressed after EPO and EVOO treatments for 10 and 21 days but EVOO had the best effect at 21 days. Conclusion: EVOO and EPO showed significant antioxidant efficacies and improved affected liver enzymes due to rheumatoid arthritis onset. When comparing olive oil has more antioxidant properties than evening primrose oil, so we recommend more studies on olive oil combination with anti-arthritic medications to improve their efficacies with less toxicity.

scholarly journals Extra Virgin Olive Oil Modulates Vasodilator Enzyme Level by Repairing Angiogenesis Function in Rat Model of Preeclampsia

2020 ◽  
Author(s):  
Yulia Silvani ◽  
Agnestia Naning Dian Lovita ◽  
Afniari Maharani ◽  
I Wayan Arsana Wiyasa ◽  
Hidayat Sujuti ◽  
...  
Keyword(s):  
Olive Oil ◽  
Rat Model ◽  
Enzyme Level ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Pregnant Rats ◽  
Vegf Level

Objective: This study aimed to determine the effect of Extra Virgin Olive Oil (EVOO) on vasodilator enzyme by repairing angiogenic function in rat model of preeclampsia. Materials and methods: This research consisted of five groups; negative control (normal pregnant rats) group, positive control (preeclampsia rat model) group, preeclampsia rat model groups given EVOO in 3 different doses (0.5 ml/day, 1 ml/day, and 2 ml/day, respectively). Blood pressure measurements were carried out on day 12, 15, and 19 of pregnancy. After the rats were sacrificed, the placentas were collected to determine endothelial Nitric Oxide Synthase (eNOS) level of maternal plasma to determine soluble Fms-like Tyrosine Kinase 1 (sFlt-1) and Vascular Endothelial Growth Factor (VEGF) level. Results: There were significant higher sFlt-1 level (p < 0.001), lower VEGF level (p = 0.009), and lower eNOS level (p = 0.034) between negative and positive control groups. After EVOO administration, sFlt-1 level was lower in dose 1 and 2 groups but higher in dose 3 group in accordance with VEGF and eNOS levels that were increasing both in dose 1 and dose 2 groups but decreasing in dose 3. There were significant differences between positive control and dose 1 (p = 0.015) and dose 2 (p = 0.001) in sFlt-1 level. None of all dose groups were statistically different with positive control group in VEGF level (dose 1 p = 0.601; dose 2 p = 0.297; dose 3 p = 0.805). eNOS levels of all dose groups were statistically different from that of the positive control group (dose 1 p = 0.014; dose 2 p = 0.001; dose 3 p = 0.024). Conclusion: Administration of EVOO modulates eNOS as vasodilator enzyme by repairing the angiogenic function indicated by decreased sFlt-1 level and increased VEGF in rat model of preeclampsia.

scholarly journals Extra Virgin Olive Oil meningkatkan ekpresi reseptor estrogen α dan ketebalan Endometrium Rattus Norvegicus yang dipapar Rhodamin B

2018 ◽  
Vol 5 (3) ◽  
pp. 201-206
Author(s):  
Ita Noviasari ◽  
I Wayan Arsana Wiyasa ◽  
Hidayat Sujuti ◽  
Eviana Norahmawati
Keyword(s):  
Oxidative Stress ◽  
Estrogen Receptors ◽  
Olive Oil ◽  
Rattus Norvegicus ◽  
Endometrial Thickness ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
The Body ◽  
Control Group ◽  
Rhodamin B

Abstract: Rhodamin B is harmful to the body because it is proven can increase the oxidative stress on various reproductive chain. Rhodamin B contains compounds of chlorine (Cl), is dangerous and halogen compounds are reactive. Damage to the hypothalamus will interfere with the secretion of FSH and LH cause the follicle ripening so distracted that result in a decrease in the hormone estrogen. The hormone estrogen plays an important role in the menstrual cycle and proliferation of the endometrium. This research aimed to prove the effect of Extra Virgin Olive Oil (EVOO) with the expression of estrogen receptors α and endometrial thickness of Rattus norvegicus exposed by rhodamin B. The design used true experimental with an approach of post test only control group design. The research used white rat (Rattus norvegicus wistar strain) as the sample. The results showed that EVOO could become an alternative to prevent oxidative stress due to exposure of rhodamin B.Keywords: Rhodamin B, Extra Virgin Olive Oil (EVOO), Estrogen receptors α, the thickness of the Endometrium

scholarly journals Histological and Weight Changes in the Testes of Plasmodium Berghei- Infected Swiss Mice Treated with Chloroquine

2021 ◽  
Vol 4 (2) ◽  
pp. 39-43
Author(s):  
N.S. Etukudoh ◽  
A.B. Deko ◽  
Uchejeso Obeta ◽  
S.K. Oyero ◽  
O.R. Ejinaka ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Weight Changes ◽  
Swiss Mice ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Chloroquine has gained great emphasis in the treatment of malaria. This study sought experimentally to determine the histomorphological and weight changes in the testes of male mice infected with Plasmodium berghei and treated with chloroquine. The study used 30 Swiss mice divided into five groups. Group 1 is Control that was not infected with Plasmodium berghei and not treated with chloroquine as control, Group 2 is Plasmodium (Plasmodiul berghei) Infected animals but not treated, Group 3 is Plasmodium Infected animals + Chloroquine (5mg/kg), Group 4 is Plasmodium Infected animals + Chloroquine (10mg/kg) and Group 5 is Plasmodium Infected animals + Chloroquine (15mg/kg). The mice were treated for 7 days after parasitaemia was confirmed and the Group 2-5 testes studied with reference to Group 1. The results showed that the Group 2 showed a little distortion, difference in spermatogenic activities and increased cellular activities; Group 3 showed large, convoluted tubules, moderate number of spermatids and large interstitial spaces, Group 4 showed Large seminiferous tubules, large spermatids, increased distortion and group 5 showed shrinking of seminiferous tubules, degeneration of interstitial cells of Leydig cells and Sertolic cells with spermatids. Groups 1-5 showed no significant effect in in body weights and testes weights of Swiss mice. Plasmodium berghei (malaria) and chloroquine have effects on histomorphological structures of Swiss mice testes but not on their teste’s weights. The testicular section from Swiss mice infected with malaria and treated at various doses when compared with the Control (Group 1) showed some moderate distortion in some structures like seminiferous tubules, connective tissues between the tubules, lumen and interstitial spaces. It can be deducted that Plasmodium berghei which caused parasitaemia in mice could cause a little tissue effect on mice if not treated.

scholarly journals Extra-Virgin Olive Oil Modifies the Changes Induced in Non-Nervous Organs and Tissues by Experimental Autoimmune Encephalomyelitis Models

Nutrients ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 2448 ◽  
Author(s):  
Cristina Conde ◽  
Begoña M. Escribano ◽  
Evelio Luque ◽  
Montserrat Feijóo ◽  
Javier Caballero-Villarraso ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Experimental Autoimmune Encephalomyelitis ◽  
Oxidative Damage ◽  
Olive Oil ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Male Rats ◽  
Dark Agouti ◽  
Autoimmune Encephalomyelitis ◽  
Experimental Autoimmune

This study reveals the existence of oxidative stress (reactive oxygen species (ROS)) in non-nervous organs and tissues in multiple sclerosis (MS) by means of a model of experimental autoimmune encephalomyelitis (EAE) in rats. This model reproduces a similar situation to MS, as well as its relationship with intestinal microbiota starting from the changes in bacterial lipopolysaccharide levels (LPS) in the outer wall of the gram-negative bacteria. Finally, the administration of extra-virgin olive oil (EVOO), hydroxytirosol (HT), and oleic acid (OA) exert beneficial effects. Twenty-five Dark Agouti two-month-old male rats, weighing around 190 g, were distributed into the following groups: Control, EAE (experimental autoimmune encephalomyelitis group), EAE + EVOO, EAE + HT, and EAE + OA. The glutathione redox system with the EAE was measured in heart, kidney, liver, and small and large intestines. The LPS and the correlation with oxidative stress in the small and large intestines were also investigated. The results showed that (1) the oxidative damage in the EAE model affects non-nervous organs and tissues; (2) The LPS is related to inflammatory phenomena and oxidative stress in the intestinal tissue and in other organs; (3) The administration of EVOO, HT, and OA reduces the LPS levels at the same time as minimizing the oxidative damage; (4) EVOO, HT, and OA improve the disease’s clinical score; and (5) on balance, EVOO offers a better neuroprotective effect.

A Randomized Clinical Trial of Greek High Phenolic Early Harvest Extra Virgin Olive Oil in Mild Cognitive Impairment: The MICOIL Pilot Study

2020 ◽  
Vol 78 (2) ◽  
pp. 801-817 ◽  
Author(s):  
Magda Tsolaki ◽  
Eftychia Lazarou ◽  
Mahi Kozori ◽  
Niki Petridou ◽  
Irene Tabakis ◽  
...  
Keyword(s):  
Cognitive Impairment ◽  
Mild Cognitive Impairment ◽  
Olive Oil ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Group 3 ◽  
Group 2 ◽  
Almost All ◽  
Early Harvest ◽  
Group 1

Background: Extra virgin olive oil (EVOO) constitutes a natural compound with high protection over cognitive function. Objective: To investigate for the first time the effect of Greek High Phenolic Early Harvest Extra Virgin Olive Oil (HP-EH-EVOO) versus Moderate Phenolic (MP-EVOO) and Mediterranean Diet (MeDi) in people with mild cognitive impairment (MCI). Methods: We conducted a randomized prospective study so as to examine the HP-EH-EVOO and MP-EVOO versus MeDi in MCI. Genetic predisposition (APOE ɛ4) to Alzheimer’s disease (AD) was tested and an extensive neuropsychological battery was administered at baseline and after 12 months. Each participant was randomized and assigned one of three groups: 1) Group 1 received the HP-EH-EVOO (50 mL/day); 2) Group 2 received the MP-EVOO (50 mL/day), and 3) Group 3 received only the MeDi instructions. Results: Better follow-up performance was found in Group 1 compared to Group 2 and Group 3 in the almost all cognitive domains. Moreover, Group 2 showed also significant improvement compared to Group 3 in ADAS-cog (p = 0.001) and MMSE (p = 0.05), whereas Group 3 exhibited worse or similar to baseline performance in almost all domains. In particular, Group 1 and Group 2 had better outcomes with regards to ADAS-cog (p = 0.003), Digit Span (p = 0.006), and Letter fluency (p = 0.003). Moreover, there was a significant difference (p = 0.001) in the presence of APOE ɛ4 between the Groups 1 and 2 versus Group 3. Conclusion: Long-term intervention with HP-EH-EVOO or MP-EVOO was associated with significant improvement in cognitive function compared to MeDi, independent of the presence of APOE ɛ4.

scholarly journals Effects of dietary extra-virgin olive oil on behaviour and brain biochemical parameters in ageing rats

2010 ◽  
Vol 103 (11) ◽  
pp. 1674-1683 ◽  
Author(s):  
Vanessa Pitozzi ◽  
Michela Jacomelli ◽  
Mohamed Zaid ◽  
Cristina Luceri ◽  
Elisabetta Bigagli ◽  
...  
Keyword(s):  
Olive Oil ◽  
Biochemical Parameters ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
High Energy ◽  
Male Rats ◽  
Phenolic Antioxidants ◽  
Total Phenols ◽  
Emotional Behaviour ◽  
The Brain

The aim of the present study was to verify whether extra-virgin olive oil, a dietary component naturally containing phenolic antioxidants, has the potential to protect the brain from the deleterious effects of ageing. To accomplish this goal, we used male rats fed a high-energy diet containing either maize oil, or extra-virgin olive oil with high or low phenol content (720 or 10 mg total phenols/kg oil, corresponding to a daily dose of 4 or 0·05 mg total phenols/kg body weight, respectively) from age 12 months to senescence. The measured endpoints were biochemical parameters related to oxidative stress and functional tests to evaluate motor, cognitive and emotional behaviour. Olive oil phenols did not exert major protective actions on motor and cognitive function, as we observed only a tendency to improved motor coordination on the rotarod in the old animals treated with the oil rich in phenols (40 % average increase in the time to first fall; P = 0·18). However, an interesting finding of the present study was a reduced step-through latency in the light–dark box test, found in the older animals upon treatment with the oil rich in antioxidant phenols, possibly indicating an anxiety-lowering effect. This effect was associated with decreased glutathione reductase activity and expression in the brain, a phenomenon previously associated with decreased anxiety in rodents. These results indicate a previously undetected effect of a diet containing an olive oil rich in phenols. Further studies are warranted to verify whether specific food antioxidants might also have an effect on emotional behaviour.

scholarly journals Efektifitas Pemberian Ekstra Virgin Minyak Zaitun Dan Ekstrak Kulit Manggis (Mastin) Terhadap Kadar Kolesterol Tikus Putih Starin Wistar Jantan Yang Diinduksi Hiperlipedemia

2017 ◽  
Vol 6 (2) ◽  
Author(s):  
Yeni Tutu Rohimah ◽  
Sri Lestari Dwi Astuti
Keyword(s):  
Olive Oil ◽  
Total Cholesterol ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Positive Control ◽  
Control Group ◽  
High Fat ◽  
Mangosteen Peel ◽  
Male Wistar Rats ◽  
Peel Extract

Abstract : Extra Virgin Olive Oil, Mangosteen Peel Extract, Serum Lipid Levels. Cardiovascular disease was the number one cause of death, risk factors of cardiovascular disease were divided into the risk factors that can’t be modified and the factors that can be modified. Some of the plants that have therapeutic benefit are olive oil and mangosteen peel extract. Olive oil is a useful food because of its content is rich in monounsaturated fatty acids (MUFA). Mangosteen peel extract has properties as antilipemic that can increase the activity of Peroxisome proliferator-Activated Receptors (PPAR). This research had a purpose to examine the effectiveness between Extra Virgin Olive Oil and the mangosteen peel extract against cholesterol levels. The methode of this research was laboratory experimental with post test only control group design. Thirty eight male wistar rats were divided into four groups. The negative control (K1) was fed with standard food for 38 days. The positive control (K2) was fed with high fat food for 38 days. The first treatment group (P1) was fed with high fat food for 38 days then it was fed with standard food and Extra Virgin Olive Oil 0,5 gr/day for 28 days. The second treatment group (P2) was fed with high fat food for 38 days then it was fed with standard food and mangosteen peel extract 0,0125 gr/days for 28 days. Afterwards, all of the groups total cholesterol and trigliserida were examined. The data analysis used One Way Anova then it tested by post-hok (p > 0,005). The result for K2 were 336,9 ± 43,76 trigliserida and 263,3 ± 12,9 total cholesterol, K1 were 138,2 ±12,7 trigliserida and 76,3 ± 8,2 total cholesterol, P1 were 325,02 ± 38,2 trigliserida and 73,6 ± 17,8 total cholesterol, P2 were 328 ± 48,14 trigliserida and 66, 5 ± 13,7 total cholesterol. The analysis result between positive control group with Extra Virgin Olive Oil and the mangosteen peel extract for total cholesterol was p < 0,05 while for trigliserida was p > 0,05. The ratio Extra Virgin Olive Oil and the mangosteen peel extract was p > 0,05. Extra Virgin Olive Oil and the mangosteen peel extract has the same effectiveness to reduce cholesterol on a male wistar rats that were induced by hyperlipidemia.

scholarly journals Cannabis sativa L. may reduce aggressive behaviour towards humans in shelter dogs

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sara Corsetti ◽  
Simona Borruso ◽  
Livia Malandrucco ◽  
Valentina Spallucci ◽  
Laura Maragliano ◽  
...  
Keyword(s):  
Olive Oil ◽  
Aggressive Behaviour ◽  
Cannabis Sativa ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Treated Group ◽  
Control Group ◽  
Focal Animal ◽  
Shelter Dogs ◽  
The Difference

AbstractAmong the phytocomplex components of Cannabis sativa L., cannabidiol (CBD) has a recognised therapeutic effect on chronic pain. Little is known about the veterinary use of CBD in dogs. Even less is known on the effects of CBD on dog behaviour, especially in shelters. The purpose of this study was to determine if CBD affects stress related behaviour in shelter dogs. The sample consisted of 24 dogs divided into two groups that were created by assigning the dogs alternately: 12 dogs were assigned to the treatment group and 12 to the control group. Extra virgin olive oil, titrated to 5% in CBD was given to treated group; the placebo consisted of olive oil only, dispensed daily for 45 days. Behavioural data were collected using the ‘focal animal’ sampling method with ‘all occurrences’ and ‘1/0’ methods for 3 h: before (T0), after 15 days (T1), after 45 days of treatment (T2) and after 15 days from the end of the treatment (T3). Treated dogs showed reduced aggressive behaviour toward humans following the treatment (Friedman Test: χ2 = 13.300; df = 3; N = 12; p = .004; adj. sig. p = 0.027), but the difference in the decrease of aggressive behaviour between the two groups was not significant (Mann–Whitney U test, T2–T0: Z = − 1.81; N = 24; p = 0.078). Other behaviours indicative of stress, such as displacing activities and stereotypes, did not decrease. Despite some non-significant results, our findings suggest that it is worth doing more research to further investigate the effect of CBD on dog behaviour; this would be certainly valuable because the potential for improving the welfare of dogs in shelters is priceless.

Toxicity of iron oxide nanoparticles, silver nanoparticles and their mixture on antioxidant enzymes and free radicals in male rats

2021 ◽  
Vol 1 (2) ◽  
pp. 009-020
Author(s):  
Ahmed Ibrahim Younus ◽  
Mokhtar Ibrahim Yousef ◽  
Maher Abdel-NabiKamel ◽  
Rakhad Alrawi ◽  
Jubran Mohammed Abdulrahman
Keyword(s):  
Antioxidant Enzymes ◽  
Thiobarbituric Acid ◽  
Male Rats ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Group 4 ◽  
Total Antioxidant ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

In the present study Wistar male rats were used. Rats were divided into 4 equal groups, 10 rats each. Group 1 served as control, group 2 was administered orally with Fe2O3NPs (5 mg/kg BW; >50 nm), group 3 was treated intraperitoneally with AgNPs (50 mg/kg BW; >100 nm) and group 4 was administered with the mixture of Fe2O3NPs with AgNPs. Animals were treated with the doses every day for 79 days. Results showed significant (P < 0.05) decrease in the antioxidant enzymes (GPX, GST, CAT and SOD) and reduced glutathione (GSH) and total antioxidant capacity (TAC), while significant (P < 0.05) increase in thiobarbituric acid-reactive substances (TBARS) and nitric oxide (NO) in plasma and testes of rats treated with Fe2O3NPs, AgNPs and their combination compared to control group.

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