scholarly journals A Pilot Study for Metabolic Profiling of Obesity-Associated Microbial Gut Dysbiosis in Male Wistar Rats

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 303
Author(s):  
Julia Hernandez-Baixauli ◽  
Pere Puigbò ◽  
Helena Torrell ◽  
Hector Palacios-Jordan ◽  
Vicent J. Ribas Ripoll ◽  
...  
Keyword(s):  
Risk Factors ◽  
Gut Microbiota ◽  
Metabolic Profile ◽  
Wistar Rats ◽  
Gut Dysbiosis ◽  
Male Wistar Rats ◽  
Obesity Risk Factors ◽  
Phenylalanine Metabolism ◽  
Host Metabolism ◽  
Obese Male

Obesity is one of the most incident and concerning disease worldwide. Definite strategies to prevent obesity and related complications remain elusive. Among the risk factors of the onset of obesity, gut microbiota might play an important role in the pathogenesis of the disease, and it has received extensive attention because it affects the host metabolism. In this study, we aimed to define a metabolic profile of the segregated obesity-associated gut dysbiosis risk factor. The study of the metabolome, in an obesity-associated gut dysbiosis model, provides a relevant way for the discrimination on the different biomarkers in the obesity onset. Thus, we developed a model of this obesity risk factors through the transference of gut microbiota from obese to non-obese male Wistar rats and performed a subsequent metabolic analysis in the receptor rats. Our results showed alterations in the lipid metabolism in plasma and in the phenylalanine metabolism in urine. In consequence, we have identified metabolic changes characterized by: (1) an increase in DG:34:2 in plasma, a decrease in hippurate, (2) an increase in 3-HPPA, and (3) an increase in o-coumaric acid. Hereby, we propose these metabolites as a metabolic profile associated to a segregated dysbiosis state related to obesity disease.

The Consumption of Galactomannan Effervescent Drinks made from Coconut Pulp Waste and Colonic Microbiota in Wistar Rats

2020 ◽  
Vol 15 (1) ◽  
pp. 52-56
Author(s):  
Sri Winarti ◽  
Agung Pasetyo
Keyword(s):  
Fatty Acids ◽  
Gut Microbiota ◽  
Wistar Rats ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
E Coli ◽  
Colonic Microbiota ◽  
Male Wistar Rats ◽  
Lactobacillus Spp ◽  
Chain Fatty Acids

The consumption of prebiotics is known to affect the balance of gut microbiota. The purpose of this study was to explore how a galactomannan-rich effervescent drink can affect the population of Lactobacillus, Bifidobacterium, E. coli, and the concentration of short-chain fatty acids in the cecum of rats. Twenty-eight male Wistar rats (aged 2 months) were divided equally into 7 groups and treated orally each day for 15 days with 2 mL effervescent drinks with increasing levels of prebiotic galactomannan. The dosage of 500 mg galactomannan increased the growth of Lactobacillus spp. and Bifidobacterium spp. with inhibition of the growth of E.coli with increased formation of short-chain fatty acids such as acetate, propionate, and butyrate in the cecum of rats.

scholarly journals Sibutramine reduces feeding, body fat and improves insulin resistance in dietary-obese male Wistar rats independently of hypothalamic neuropeptide Y

2001 ◽  
Vol 132 (8) ◽  
pp. 1898-1904 ◽  
Author(s):  
Michael Brown ◽  
Chen Bing ◽  
Peter King ◽  
Lucy Pickavance ◽  
David Heal ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Neuropeptide Y ◽  
Body Fat ◽  
Wistar Rats ◽  
Male Wistar Rats ◽  
Obese Male

scholarly journals Effect of 4-h time restricted feeding on body weight, leptin concentration and lipid profile in healthy non-obese male Wistar rats

2021 ◽  
Author(s):  
Michael A. Olamoyegun ◽  
Folasade O. Ajao ◽  
Marcus O. Iyedupe
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Metabolic Diseases ◽  
Restricted Feeding ◽  
Mean Values ◽  
Male Wistar Rats ◽  
Ad Libitum ◽  
Obese Male

Abstract Background: Obesity greatly increases the risk of metabolic diseases and preventive approaches for obesity are often inadequate to effectively prevent and manage the diseases. Altering feeding time strategy intervention decreases caloric intake without calorie counting and may be an effective therapy. Therefore, this study investigates the effect of 4-h time restricted feeding on body weight, leptin concentration and lipid profile in healthy non-obese male Wistar rats. Methods: Rats placed on time-restricted feeding (TRF) regimen had freely access to food for 4 hour per day at designated periods. Twenty four rats divided into four groups (n=6) were used. Group I animals were placed on a 4 hour per day TRF between 8am-12noon. Group II rats were also placed on a 4 hour per day TRF between 12noon-4pm. Group III rats also placed on a 4 hour per day TRF between 8pm-12 midnight while Group IV rats had access food and water ad libitum. This diet strategy resembles taking only breakfast, lunch or dinner once a day. The study lasted for a period of 4 weeks with daily food intake and weekly body weight determined throughout the period. At the end of the experimental period, blood glucose, lipid profile and leptin concentration were assessed. SPSS 21.0 package was used for data analysis, one-way analysis of variance (ANOVA) was used to compare the mean values of variables among the groups and bonferroni’s posthoc test was used for significance of pair wise comparisons of mean values among the groups. Significance was set at p < 0.05.Results: In this study, the body weights and leptin concentrations of 8pm – 12am and ad libitum groups significantly increased compared with the 8am - 12noon and 12noon -4pm groups. Dyslipidemia was observed in the ad libitum group when compared with the 8am - 12noon and 12noon - 4pm groups. Conclusion: From this study, 4-hr time restricted feeding has beneficial effects on body weight, blood glucose, lipid profile and leptin concentration. This feeding restriction patterns may be helpful in obesity management and in preventing metabolic diseases development in non obese.

Beneficial effects of lipidic extracts of saladette tomato pomace andSerenoa repenson prostate and bladder health in obese male Wistar rats

2017 ◽  
Vol 97 (13) ◽  
pp. 4451-4458 ◽  
Author(s):  
Josué V Espinosa-Juárez ◽  
Juventino III Colado-Velázquez ◽  
Patrick Mailloux-Salinas ◽  
JML Medina-Contreras ◽  
P Valentín Correa-López ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Tomato Pomace ◽  
Beneficial Effects ◽  
Male Wistar Rats ◽  
Obese Male

scholarly journals Experimental hypogonadism: insulin resistance, biochemical changes and effect of testosterone substitution

2019 ◽  
Vol 30 (3) ◽  
Author(s):  
Ilias P. Doulamis ◽  
Aspasia Tzani ◽  
Panagiotis Konstantopoulos ◽  
Afroditi Daskalopoulou ◽  
Theodoros Spinos ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Lipid Profile ◽  
Metabolic Profile ◽  
Wistar Rats ◽  
Sham Operation ◽  
Blood Samples ◽  
Testosterone Substitution ◽  
Negative Effect ◽  
Male Wistar Rats

Abstract Background We sought to clarify the role of testosterone substitution in terms of insulin resistance and metabolic profile dysregulation in hypogonadism. Methods Twenty-nine male Wistar rats aged 11–12 weeks were divided in three groups: control (C, n = 10), sham operation; orchiectomy (ORX, n = 9); and orchiectomy + testosterone substitution (ORX+T, n = 10). Blood samples were obtained at day 1 (operation), after 10 days (intramuscular T injection 100 μg/100 g b.w.), 25 days (second T injection) and 40 days (sacrifice). Results Hormonal replacement significantly attenuated the negative effect of orchiectomy on insulin resistance as indicated by the successive changes in both insulin levels (1.44 ± 2.94 vs. 4.10 ± 2.47 vs. 1.78 ± 0.68 ng/mL, for D1, D10 and D40, respectively; p = 0.028 and p = 0.022, respectively) and HOMA-IR index (1.36 ± 2.75 vs. 3.68 ± 1.87 vs. 1.74 ± 0.69 ng/mL, for D1, D10 and D40, respectively; p = 0.024 and p = 0.026, respectively) in the ORX+T group. Irisin levels peaked at the 10th postoperative day and were decreased at the end of the experiment (0.27 ± 0.11 vs. 0.85 ± 0.54 vs. 0.02 ± 0.07 ng/mL for D1, D10 and D40, respectively; p = 0.028 in both cases), whereas resistin levels did not differ. Experimental hypogonadism results in an unfavorable lipid profile and insulin resistance, which is not observed when the ORX animals are substituted for T.

scholarly journals Monosodium Glutamate Induces Changes in Hepatic and Renal Metabolic Profiles and Gut Microbiome of Wistar Rats

Nutrients ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1865
Author(s):  
Kanokwan Nahok ◽  
Jutarop Phetcharaburanin ◽  
Jia V. Li ◽  
Atit Silsirivanit ◽  
Raynoo Thanan ◽  
...  
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
16S Rrna Gene ◽  
Gut Microbiome ◽  
Wistar Rats ◽  
Monosodium Glutamate ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Male Wistar Rats ◽  
The Impact

The short- and long-term consumption of monosodium glutamate (MSG) increases urinary pH but the effects on the metabolic pathways in the liver, kidney and the gut microbiota remain unknown. To address this issue, we investigated adult male Wistar rats allocated to receive drinking water with or without 1 g% MSG for 2 weeks (n = 10, each). We performed a Nuclear Magnetic Resonance (NMR) spectroscopy-based metabolomic study of the jejunum, liver, and kidneys, while faecal samples were collected for bacterial DNA extraction to investigate the gut microbiota using 16S rRNA gene sequencing. We observed significant changes in the liver of MSG-treated rats compared to controls in the levels of glucose, pyridoxine, leucine, isoleucine, valine, alanine, kynurenate, and nicotinamide. Among kidney metabolites, the level of trimethylamine (TMA) was increased, and pyridoxine was decreased after MSG-treatment. Sequencing of the 16S rRNA gene revealed that MSG-treated rats had increased Firmicutes, the gut bacteria associated with TMA metabolism, along with decreased Bifidobacterium species. Our data support the impact of MSG consumption on liver and kidney metabolism. Based on the gut microbiome changes, we speculate that TMA and its metabolites such as trimethylamine-N-oxide (TMAO) may be mediators of the effects of MSG on the kidney health.

scholarly journals Pituitary growth hormone release and gene expression in cafeteria-diet-induced obese rats

1998 ◽  
Vol 159 (1) ◽  
pp. 165-172 ◽  
Author(s):  
X Zhou ◽  
J De Schepper ◽  
D De Craemer ◽  
M Delhase ◽  
G Gys ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wistar Rats ◽  
Transcript Level ◽  
Cafeteria Diet ◽  
Male Rats ◽  
Pituitary Cells ◽  
Obese Rats ◽  
Igf I ◽  
Male Wistar Rats ◽  
Obese Male

In human obesity as well as in rat obesity models a decrease in spontaneous and stimulated GH secretion has been a constant finding. The presence of a decreased pituitary GH synthesis in diet-induced obese male rats was investigated and its possible relationship with obesity-related changes in peripheral hormones was analyzed. Cafeteria-diet-overfed obese male Wistar rats with body fat percentage above 30% had a significantly decreased pituitary GH mRNA transcript level assessed by both Northern blot and in situ hybridization, and a lower pituitary GH protein level as demonstrated by immunocytochemistry. The GH transcript level correlated negatively with the serum leptin and positively with the IGF-I concentration. No differences in circulating tri-iodothyronine, non-fasting insulin and corticosterone levels were found between overfed and control rats. GH release by cultured pituitary cells from overfed rats was comparable to that by cells prepared from control rats. In contrast, incubation of normal pituitary cells with serum from overfed rats for 3 days gave a significantly lower GH release than after incubation with serum from non-obese rats. In conclusion, cafeteria-diet-induced obese male Wistar rats have a decreased pituitary GH gene expression and a modifiable GH release in in vitro experiments. A possible role for peripheral circulating factors, like leptin and IGF-I, in decreasing the pituitary GH synthesis and release in obese rats is discussed.

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