scholarly journals An Emerging Anti-p16 Antibody-BC42 Clone as an Alternative to the Current E6H4 for Use in the Female Genital Tract Pathological Diagnosis: Our Experience and a Review on p16ink4a Functional Significance, Role in Daily-Practice Diagnosis, Prognostic Potential, and Technical Pitfalls

Diagnostics ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 713
Author(s):  
Giuseppe Angelico ◽  
Angela Santoro ◽  
Frediano Inzani ◽  
Patrizia Straccia ◽  
Saveria Spadola ◽  
...  
Keyword(s):  
Female Genital Tract ◽  
Daily Practice ◽  
Cervical Squamous Cell Carcinoma ◽  
High Grade ◽  
The Novel ◽  
Medical Systems ◽  
Ovarian Carcinomas ◽  
Study Results ◽  
Diagnostic Applications ◽  
Gynecologic Neoplasms

Background: To date, useful diagnostic applications of p16 IHC have been documented in gynecological pathology both for HPV-related and non-HPV-related lesions. In the present article, we reported our experience with the novel anti-p16 INK4a antibody (clone BC42), whose expression was tested across all different gynecologic neoplasms; we also compared it to the traditional E6H4 clone. Moreover, we discussed and explored all the diagnostic applications of p16 IHC in gynecologic pathology. Methods: Consultation cases covering a 5-year period (2016–2020) regarding gynecological neoplastic and non-neoplastic lesions in which immunohistochemistry for p16, clone E6H4 was originally performed, were retrospectively retrieved from the files of our institution. Immunohistochemical staining for p16ink4a (BC42) [Biocare Medical group-Paceco USA; Bioptica Milan] and p16ink4a (E6H4) [Ventana Medical Systems-Arizona USA; Roche] was performed by using the Ventana automated immunostainer (Ventana Medical Systems, Tucson, AZ, USA). The immunostaining pattern was defined as negative, focal/patchy, or diffuse. Results: A total of 196 cases, represented by 36 high-grade SIL/CIN3 of the uterine cervix, 30 cervical adenocarcinomas, 22 cervical squamous cell carcinoma, 70 endometrial carcinomas, 25 high grade serous ovarian carcinomas, 6 uterine adenomatoid tumors, and 10 uterine leiomyosarcomas were included in this study. Results showed concordant staining quality of both clones on all tested neoplastic tissues. Conclusions: The novel anti-p16 antibody (BC42 clone) appeared as an alternative to the current E6H4 for use in gynecological neoplasms, offering similar levels of positivity and equally reliable staining results.

Morphological and Immunohistochemical Biomarkers in Distinguishing Prostate Carcinoma and Urothelial Carcinoma: A Comprehensive Review

2018 ◽  
Vol 27 (2) ◽  
pp. 120-133 ◽  
Author(s):  
Francesca Sanguedolce ◽  
Davide Russo ◽  
Vito Mancini ◽  
Oscar Selvaggio ◽  
Beppe Calò ◽  
...  
Keyword(s):  
Urothelial Carcinoma ◽  
Prostate Carcinoma ◽  
Daily Practice ◽  
Comprehensive Analysis ◽  
High Grade ◽  
Clinical Context ◽  
Comprehensive Review ◽  
Immunohistochemical Markers ◽  
Diagnostic Applications ◽  
Accuracy Rates

The differential diagnosis between high-grade prostate carcinoma and infiltrating urothelial carcinoma (UC) in transurethral resection prostate specimens as well as cystoprostatectomy specimens may often be challenging due to morphologic and clinical overlap of the 2 entities. Such distinction has critical therapeutic and staging consequences, yet it is hampered by both issues in morphology and by the low accuracy rates of single immunohistochemical markers, as reported in literature. This review aims to provide a comprehensive analysis of the available morphological and immunohistochemical parameters, which may allow to discriminate between prostate carcinoma and urothelial carcinoma in the proper clinical context and to discuss their diagnostic applications in daily practice.

Representasi Sikap Kebangsaan Anak Muda dalam Hegemoni Negara pada Novel Populer

Metahumaniora ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 100
Author(s):  
Muhamad Adji
Keyword(s):  
New Order ◽  
The Novel ◽  
The Third ◽  
Study Results ◽  
Popular Novel

AbstrakPenelitian ini bertujuan untuk memperoleh gambaran tentang sikap kebangsaananak muda Indonesia pada tiga novel populer yaitu Ali Topan Anak Jalanan karya TeguhEsha, Lupus: Makhluk Manis dalam Bis karya Hilman Hariwijaya, dan Balada Si Roy karya GolaGong. Alasan pemilihan objek penelitian di atas adalah karena ketiga novel tersebut memilikitingkat popularitas yang tinggi pada tiap zamannya sehingga membuat tokoh utamanyamenjadi representasi anak muda Indonesia pada zamannya masing-masing. Pertanyaanpertanyaanyang memandu tulisan ini adalah 1) Bagaimana keindonesiaan digambarkanpada ketiga novel tersebut dan 2) Bagaimana sikap kebangsaan direpresentasikan tokohanak muda pada ketiga novel tersebut. Dari hasil kajian didapatkan simpulan bahwasikap kebangsaan yang ditampilkan pada ketiga novel tersebut memiliki tingkatan yangberbeda-beda. Novel Ali Topan Anak Jalanan menunjukkan sikap kebangsaan dengan lebihkritis. Hegemoni orde Baru pada masa itu belum berhasil karena novel populer masihmenampilkan tokoh anak muda yang kritis dan cenderung memberontak terhadap nilainilaiideologi Orde Baru. Periode ’80-an menampilkan novel populer dengan tokoh anakmuda yang memiliki sikap kebangsaan yang terbelah. Pertama, anak muda yang dapatmenerima realitas yang dikontruksi Orde Baru, dengan menjalani sistem tersebut danmemposisikan dirinya sebagai bagian dari sistem tersebut. Hal itu direpresentasikan olehtokoh Lupus dalam novel Lupus: Makhluk Manis dalam Bis. Kedua, anak muda yang tidakdapat menerima realitas yang dikonstruksi Orde Baru, dengan cara keluar dari bagiansistem tersebut. Hal itu direpresentasikan oleh tokoh Roy dalam Balada Si Roy.Kata kunci: kebangsaan, anak muda, novel populer, Orde BaruAbstractThis study aims to obtain a picture of Indonesian nationalism in the three popularnovels that were Ali Topan Anak Jalanan by Teguh Esha, Lupus: Makhluk Manis dalam Bisby Hilman Hariwijaya, dan Balada Si Roy by Gola Gong. The reason for choosing the object ofresearch above is that all three novels are recognized to have high levels of popularity in eachera so that made the main characters became a representation of Indonesian youth at that time.The questions that guide the writing are 1) How Indonesianness described in the third novel?2) How does nationalism represented by youth in these three novels. From the study results isobtained the conclusion that nationalism is displayed on the third novel has a level different.Ali Topan Anak Jalanan show a nationalism more critically. The New Order’s hegemony atthat time has not been successful because the novel still showing a critical youth and tends torevolt against the ideological values of the New Order. The ‘80s period featured a popular novelwith a youth character who had a split nationalism. First, the youth who can accept the realitythat the New Order has constructed, with the system and position itself as part of the system.It was represented by the Lupus’s character in the Lupus: Makhluk Manis dalam Bis. Second,the youth who can not accept the facts constructed by the New Order, by going out from thatpart of the system. It was represented by Roy’s character in the Balada Si Roy.Keywords: nationalism, youth, popular novel, New Order

MOLECULAR AND GENETIC SUBTYPES OF OVARIAN CANCER: PROSPECTS FOR FURTHER STUDIES

2019 ◽  
Vol 65 (1) ◽  
pp. 56-62
Author(s):  
Alisa Villert ◽  
Larisa Kolomiets ◽  
Natalya Yunusova ◽  
Yevgeniya Fesik
Keyword(s):  
Ovarian Cancer ◽  
Ovarian Carcinoma ◽  
Molecular Subtypes ◽  
Survival Rates ◽  
Consensus Algorithm ◽  
Histopathological Diagnosis ◽  
Low Grade ◽  
High Grade ◽  
Ovarian Carcinomas ◽  
Genetic Subtypes

High-grade ovarian carcinoma is a histopathological diagnosis, however, at the molecular level, ovarian cancer represents a heterogeneous group of diseases. Studies aimed at identifying molecular genetic subtypes of ovarian cancer are conducted in order to find the answer to the question: can different molecular subgroups influence the choice of treatment? One of the achievements in this trend is the recognition of the dualistic model that categorizes various types of ovarian cancer into two groups designated high-grade (HG) and low-grade (LG) tumors. However, the tumor genome sequencing data suggest the existence of 6 ovarian carcinoma subtypes, including two LG and four HG subtypes. Subtype C1 exhibits a high stromal response and the lowest survival. Subtypes C2 and C4 demonstrate higher number of intratumoral CD3 + cells, lower stromal gene expression and better survival than sybtype C1. Subtype C5 (mesenchymal) is characterized by mesenchymal cells, over-expression of N-cadherin and P-cadherin, low expression of differentiation markers, and lower survival rates than C2 and C4. The use of a consensus algorithm to determine the subtype allows identification of only a minority of ovarian carcinomas (approximately 25%) therefore, the practical importance of this classification requires additional research. There is evidence that it makes sense to randomize tumors into groups with altered expression of angiogenic genes and groups with overexpression of the immune response genes, as in the angiogenic group there is a comparative superiority in terms of survival. The administration of bevacizumab in the angiogenic group improves survival, while the administration of bevacizumab in the immune group even worsens the outcome. Molecular subtypes with worse survival rates (proliferative and mesenchymal) also benefit most from bevacizumab treatment. This review focuses on some of the advances in understanding molecular, cellular, and genetic changes in ovarian carcinomas with the results achieved so far regarding the formulation of molecular subtypes of ovarian cancer, however further studies are needed.

scholarly journals Epstein-Barr Virus Versus Novel Coronavirus-Induced Hemophagocytic Lymphohistocytosis: The Uncharted Waters

2020 ◽  
Vol 8 ◽  
pp. 232470962095010 ◽  
Author(s):  
Rawan Amir ◽  
Asim Kichloo ◽  
Jagmeet Singh ◽  
Ravinder Bhanot ◽  
Michael Aljadah ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Epstein Barr Virus ◽  
Genetic Mutations ◽  
High Grade ◽  
The Novel ◽  
Barr Virus ◽  
First Case ◽  
Epstein Barr ◽  
Virus Versus ◽  
Novel Coronavirus

Hemophagocytic lymphohistocytosis (HLH) is a hyperinflammatory syndrome characterized by fever, hepatosplenomegaly, and pancytopenia. It may be associated with genetic mutations or viral/bacterial infections, most commonly Epstein-Barr virus (EBV) and cytomegalovirus. As for the novel coronavirus, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), also known as COVID-19 (coronavirus disease-2019), the cytokine storm it triggers can theoretically lead to syndromes similar to HLH. In this article, we report a case of a 28-year-old female who presented with high-grade fevers, found to have both SARS-CoV-2 and EBV infections, and eventually began to show signs of early HLH. To our knowledge, this is the first case reported in literature that raises the possibility of SARS-CoV-2–related HLH development.

scholarly journals A Study On Financial Self-Efficacy During Covid-19

2021 ◽  
Vol 23 (08) ◽  
pp. 472-483
Author(s):  
Sitangshu Khatua ◽  
◽  
Debdulal Dutta Roy ◽  
Keyword(s):  
Self Efficacy ◽  
Principal Component ◽  
The Novel ◽  
The Great Depression ◽  
Online Mode ◽  
Personal Finances ◽  
Study Results ◽  
Financial Goal ◽  
Novel Coronavirus ◽  
And Control

Financial Self-efficacy is defined as a person’s observed capability to control his/her personal finances (Lapp, 2010; Postmus, 2011). It refers to one’s beliefs in the abilities to accomplish a financial goal or task. It is the “knowledge and ability to influence and control one’s financial matters” by Fox and Bartholomae (2008). Financial efficacy pattern of people during very critical moment is unknown. The world is experiencing one of the deepest recessions since the Great Depression in the 1930s owing to the novel coronavirus, World Bank President David Malpass has said, terming the COVID-19 pandemic a “catastrophic event” for many developing and the poorest countries. Aim of the study is to examine financial efficacy pattern of people during lockdown period for COVID-19. Data were collected through online mode using financial efficacy scale developed by authors for the study. Results of principal component analysis revealed that during lockdown, financial efficacy is more concerned with financial planning, planned payment and financial coping.

scholarly journals The Expression of Chromogranin A, Syanptophysin and Ki67 in Detecting Neuroendocrine Neoplasma at High Grade Colorectal Adenocarcinoma

2021 ◽  
Vol 9 (A) ◽  
pp. 1142-1147
Author(s):  
W. A. Gusti Deasy ◽  
M. Husni Cangara ◽  
Andi Alfian Zainuddin ◽  
Djumadi Achmad ◽  
Syarifuddin Wahid ◽  
...  
Keyword(s):  
Chromogranin A ◽  
Colorectal Adenocarcinoma ◽  
Final Diagnosis ◽  
Neuroendocrine Neoplasm ◽  
Neuroendocrine Neoplasms ◽  
High Grade ◽  
Neuroendocrine Markers ◽  
Study Results ◽  
Cell Neoplasm ◽  
Sample Characteristics

BACKGROUND: Neuroendocrine neoplasm (NEN) is an epithelial cell neoplasm that can give a histopathological appearance resembling high-grade colorectal adenocarcinoma. Immunohistochemical assays with specific neuroendocrine markers of chromogranin A and synaptophysin are required to establish a definite diagnosis of NEN. AIM: This study aimed to determine whether there was an expression of chromogranin A, synaptophysin and Ki67 which indicated the presence of neuroendocrine neoplasms in samples that have been diagnosed as high-grade colorectal adenocarcinoma. MATERIALS AND METHODS: A study of the expression of chromogranin A, synaptophysin and Ki67 in paraffin blocks was carried out as a result of biopsy and tissue surgery of 70 samples of colorectal tumor specimens diagnosed with colorectal adenocarcinoma. Descriptive analyses were used to assess the study results of the amount of chromogranin A, synaptophysin, and sample characteristics. RESULTS: We discovered that eight (8) samples (11.4%) were NEN from 70 previously diagnosed samples as high-grade colorectal adenocarcinoma using immunohistochemical assay with neuroendocrine markers, namely chromogranin A and synaptophysin. CONCLUSION: The final diagnosis obtained from 8 samples diagnosed as NEN were Neuroendocrine tumor (NET) G1, G2, and G3, respectively 1.4% and LCNEC 7.1% based on the specific neuroendocrine markers of chromogranin A, synaptophysin and Ki67.

scholarly journals Targetable Immune Regulatory Molecule Expression in High-Grade Serous Ovarian Carcinomas in African American Women

2019 ◽  
Vol 38 (2) ◽  
pp. 157-170 ◽  
Author(s):  
Anne M. Mills ◽  
Lauren C. Peres ◽  
Alice Meiss ◽  
Kari L. Ring ◽  
Susan C. Modesitt ◽  
...  
Keyword(s):  
African American ◽  
African American Women ◽  
High Grade ◽  
Ovarian Carcinomas ◽  
American Women ◽  
Regulatory Molecule

SP6.1.2 Colorectal Liver Metastases - Novel Assessment Tools for Resectability (The CoNoR Study): Results from an International Questionnaire of Hepatopancreatobiliary Surgeons

2021 ◽  
Vol 108 (Supplement_7) ◽  
Author(s):  
Kat Parmar ◽  
Derek O'Reilly ◽  
Rob Jones ◽  
Fady Balaa ◽  
Fenella Welsh ◽  
...  
Keyword(s):  
Decision Making ◽  
Liver Metastases ◽  
Colorectal Liver Metastases ◽  
Community Support ◽  
Assessment Tools ◽  
Response Analysis ◽  
Free Text ◽  
The Novel ◽  
Liver Remnant ◽  
Study Results

Abstract Aims Hepatic resection offers the only chance of cure for colorectal liver metastases (CLM), yet wide variation in resectability decision-making has been demonstrated. This study aims to evaluate the potential value of two novel assessment tools in aiding resectability decision-making: the LiMAx test (hepatic functional capacity) and HepaT1ca interactive pre-operative MR scan (MR-based volumetry and functional assessment). Methods This study utilises four workstreams: WS1 systematic review, WS2 international HPB interviews, WS3 international HPB online questionnaire, and WS4 online scenario-based survey to assess change in decision-making resulting from the novel tools. The WS3 questionnaire closed in January 2021; participation was increased by professional association endorsement (AUGIS, GBIHPBA, E-AHPBA, AHPBA, CHPBA, IHPBA). Results 197 complete responses were received from 37 countries in 6 continents. The clinical scenario in which HPB surgeons found resectability decisions most challenging was post-chemotherapy downsizing, with >90% of respondents agreeing that the following scenarios also present a challenge: recurrent disease post-liver resection, post-portal vein embolisation, and close proximity to major ducts/vessels. Substantial variation was demonstrated in the percentage future liver remnant at which surgeons preferred further investigation in all scenarios. >90% of respondents felt the novel tools would be potentially useful in decision-making; wide-ranging free-text feedback was also provided. Conclusions The questionnaire lends support to the previously documented variation in resectability decision-making and confirms international HPB community support for investigation of these novel tools. Response analysis has facilitated the appropriate case selection to best assess their potential utility in the WS4 survey, due to launch this spring.

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