scholarly journals Renal and Inflammation Markers—Renalase, Cystatin C, and NGAL Levels in Asymptomatic and Symptomatic SARS-CoV-2 Infection in a One-Month Follow-Up Study

Diagnostics ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 108
Author(s):  
Natalia Serwin ◽  
Elżbieta Cecerska-Heryć ◽  
Ewa Pius-Sadowska ◽  
Karol Serwin ◽  
Anna Niedźwiedź ◽  
...  
Keyword(s):  
Kidney Function ◽  
Cystatin C ◽  
Asymptomatic Infection ◽  
Time Points ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Immune Parameters ◽  
Group 3 ◽  
Group 2 ◽  
Neutrophil Gelatinase

The aim of our study was to evaluate the influence of asymptomatic infection and the occurrence of symptomatic COVID-19 on specific biochemical, renal, and immune parameters—renalase, neutrophil gelatinase-associated lipocalin (NGAL) cystatin C (CysC), and creatinine—and their weekly fluctuations during a one-month observation period in COVID-19 patients admitted to hospital. The study involved 86 individuals: 30 patients with diagnosed COVID-19, 28 people with asymptomatic infection confirmed with IgG antibodies—the IG(+) group—and 28 individuals without any (IgG, IgE) anti-SARS-CoV-2 antibodies—the IG(−) group. In the COVID-19 group, blood was drawn four times: (1) on day 0/1 after admission to hospital (C1 group), (2) 7 days later (C7 group), (3) 14 days later (C14 group), and (4) 28 days later (C28 group). In the IG(−) and IG(+) groups, blood was drawn once. There were no significant differences in creatinine, Cys C, and uric acid between any of the analyzed groups. NGAL levels were significantly higher in IG(+) and at all time-points in the COVID-19 groups than in controls. A similar observation was made for renalase at the C7, C14, and C28 time-points. Plasma renalase, NGAL, and CysC are unrelated to kidney function in non-critically ill COVID-19 patients and those with asymptomatic infection. Renalase and NGAL are most likely related to the activation of the immune system rather than kidney function. Asymptomatic SARS-CoV-2 infection causes a rise in plasma NGAL levels similar to those observed in symptomatic COVID-19 patients. Therefore, more attention should be paid to tracking and monitoring the health of these people.

STUDY OF SERUM NEUTROPHIL GELATINASE ASSOCIATED LIPOCALIN CONCENTERATIONS AS A MARKER OF RENAL FUNCTION IN CHRONIC KIDNEY DISEASE PATIENTS

2021 ◽  
pp. 189-190
Author(s):  
G.G. Kaushik ◽  
Shubham Maheshwari ◽  
Ankita Sharma
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Serum Creatinine ◽  
Kidney Function ◽  
Cystatin C ◽  
Kidney Injury ◽  
Lipocalin 2 ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Neutrophil Gelatinase ◽  
Sensitive Marker

Introduction: Serum lipocalin 2 serve as a marker for kidney function. Lipocalin 2 is found in both CKD and kidney injury and it rises in acute kidney injury (AKI) and in patients have faster decline in kidney function. Aims And Objectives: To nd out correlation and assess of serum Neutrophil gelatinase-associated lipocalin 2 (NGAL 2) in patients with stages 2 to 4 of Chronic Kidney disease. The aim of the study was NGAL could represent a novel, sensitive marker of kidney function in adult patients with CKD. Material And Methods: Study involved 120 patients divided in Case group (60 patients) attended medical/ urology OPD or admitted in medical/urology ward of CKD2 – CKD4 while control group – age and sex matched healthy individuals/ stage I CKD patients was taken as control. The plasma/ serum were used for serum urea, creatinine, Cystatin C and lipocalin 2 under all aseptic precaution on receiving consent. Result:The patients of CKD included in study were having glomerulonephritis (46.7%), pyelonephritis (21.7%), diabetic kidney disease (13.3%), polycystic kidney disease (1.7%) and other causes (16.7%). CKD patients demonstrated elevated serum NGAL 159.14 ± 48.73 ng/ml, together with a rise in urea 59.9 ± 17.6 mg/dL, serum creatinine 1.56 ± 0.97 mg/dL and Cystatin C 199 ± 113 ng/ml as compared to control have serum NGAL 76.31 ± 26.34 ng/ml, urea 22.3 ± 5.7 mg/dL, serum creatinine 0.75 ± 0.14 mg/dL and Cystatin C 76 ± 17 ng/ml (P value <0.05). Conclusion: Serum NGAL closely correlates with serum Cystatin C, creatinine, and eGFR, and serve as a potential early and sensitive marker of impaired kidney function/ kidney injury.

Predictive Value of Minimal Residual Disease: Analysis By Flow-Cytometry in Children with Relapsed Acute Lymphoblastic Leukemia

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 2494-2494
Author(s):  
Myriam Ruth Guitter ◽  
Jorge Gabriel Rossi ◽  
Elisa Sajaroff ◽  
Carolina Carrara ◽  
Pizzi Silvia ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Residual Disease ◽  
Lymphoblastic Leukemia ◽  
P Value ◽  
Time Points ◽  
Group 3 ◽  
Group 2 ◽  
To Receive ◽  
Group 1

Abstract Introduction: Despite the advances observed in the outcome of pediatric acute lymphoblastic leukemia (ALL) treatment during the last 20 years, relapse remains the most common cause of treatment failure in childhood ALL. Several factors have been associated to the prognosis of these patients; however, minimal residual disease (MRD) emerges as a relevant predictor of outcome. Objectives: The aims of this study were to assess MRD by flow-cytometry in relapsed ALL and to evaluate its prognostic impact as a predictor factor of outcome at the end of the induction therapy and prior to hematopoietic stem cell transplantation (HSCT). Patients and Methods: From Aug'10 to Jun'15, 123 ALL patients were treated at our center. MRD determination at least at two time-points during relapse treatment was a requirement for considering a patient eligible for the present study. Sixty-six cases were excluded due to the following causes: 10 patients died during induction, 2 died early in complete remission (CR), 29 did not respond to chemotherapy, in 13 patients MRD determination was not performed: 4 did not have clinical data available, 4 patients were Down Syndrome and 4 children received treatment for relapse in other centers. Thus, fifty-seven patients achieved CR and were evaluated for MRD at two time points. Of them, 56 patients belonged to S4 and S3 and 1 patient to S1 group as defined by the Berlin-Frankfurt-Münster stratification for relapsed ALL. MRD was analyzed by multiparametric flow-cytometry following ALL-IC 2009 guidelines. Negative MRD was defined as disclosing less than 0.1% of blasts. For this analysis, patients were stratified based on MRD levels at two different time points: after end of induction, before HSCT or at any other time point during the follow-up for patients who did not undergo HSCT. Three groups were defined: Group-1: negative at both time points (n= 23), Group-2: positive at 1 time point (n= 13) and Group-3: positive at both time points (n= 21). Patients who relapsed before receiving HSCT were considered Group-3. Twenty-five patients underwent HSCT: 13 of them from Group-1, 9 from Group-2 (2 had positive MRD previous to receive HSCT) and 3 patients from Group-3. HSCT was performed with matched familiar donor in 16 cases and matched unrelated donor in 9 cases. Results: The distribution of events according to receiving or not HSCT was: 5 died due to transplant related mortality (TRM), 9 relapsed after receiving HSCT and 16 during treatment with chemotherapy. With a median follow-up of 16 (range: 6-67) months, overall 3-year EFS probability (EFSp) (SE) was 32 (8)%. The 3-year EFSp was 75 (11)% for Group-1, 24 (14)% for Group-2 and 0% for Group-3 (p-value <0.00001). Comparing patients who did not receive HSCT vs. patients who did, EFSp (SE) was 32 (12)% and 29 (11)% respectively (p-value: non-significant). The EFSp (SE) according to MRD groups in patients who underwent HSCT was: Group-1: 53 (19)%, Group-2: 14 (13)% and 0% for Group-3 (p-value: 0.06). Conclusions: MRD quantification by flow-cytometry demonstrated to be a significant prognostic factor for relapsed ALL. Both, TRM and death in CR rates, were high and should be decreased by improving supportive measures. MRD determination by flow-cytometry in patients who underwent HSCT showed a trend to achieve a better EFSp, thus representing a relevant tool for stratifying relapsed ALL patients in order to achieve a better selection of patients to receive HSCT. Disclosures No relevant conflicts of interest to declare.

scholarly journals Cystatin C and α-1-Microglobulin Predict Severe Acute Kidney Injury in Patients with Hemorrhagic Fever with Renal Syndrome

Pathogens ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 666 ◽  
Author(s):  
Magnus Hansson ◽  
Rasmus Gustafsson ◽  
Chloé Jacquet ◽  
Nedia Chebaane ◽  
Simon Satchell ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Cystatin C ◽  
Area Under The Curve ◽  
Kidney Injury ◽  
Hemorrhagic Fever ◽  
Roc Curve Analysis ◽  
Abrupt Decrease ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Neutrophil Gelatinase

Puumala orthohantavirus causes hemorrhagic fever with renal syndrome (HFRS) characterized by acute kidney injury (AKI), an abrupt decrease in renal function. Creatinine is routinely used to detect and quantify AKI; however, early AKI may not be reflected in increased creatinine levels. Therefore, kidney injury markers that can predict AKI are needed. The potential of the kidney injury markers urea, cystatin C, α1-microglobulin (A1M) and neutrophil gelatinase-associated lipocalin (NGAL) to detect early AKI during HFRS was studied by quantifying the levels of these markers in consecutively obtained plasma (P) and urine samples (U) for 44 HFRS patients. P-cystatin C and U-A1M levels were significantly increased during early HFRS compared to follow-up. In a receiver operating characteristic (ROC) curve analysis, P-cystatin C, U-A1M and P-urea predicted severe AKI with area under the curve 0.72, 0.73 and 0.71, respectively, whereas the traditional kidney injury biomarkers creatinine and U-albumin did not predict AKI. Nearly half of the HFRS patients (41%) fulfilled the criteria for shrunken pore syndrome, which was associated with the level of inflammation as measured by P-CRP. P-cystatin C and U-A1M are more sensitive and earlier markers compared to creatinine in predicting kidney injury during HFRS.

scholarly journals Ureteric stenting versus non-stenting following uncomplicated ureteroscopic lithotripsy: A Prospective randomized trial.

2019 ◽  
Author(s):  
Saddam Al Demour ◽  
Adel Alrabadi ◽  
Abedallatif AlSharif ◽  
Mera Ababneh ◽  
Motaz Melhem ◽  
...  
Keyword(s):  
Operative Time ◽  
Flank Pain ◽  
Ureteroscopic Lithotripsy ◽  
Time Points ◽  
Ureteric Stenting ◽  
Significant Difference ◽  
Analgesia Requirement ◽  
Group 3 ◽  
Group 2

Abstract Background: There is no consensus about whether a double-J ureteric stent (DJ-US) should be placed following uncomplicated ureteroscopy for stone retrieval. This study aimed to compare three groups of patients who underwent uncomplicated ureteroscopic lithotripsy (URSL) and to evaluate whether stents could be eliminated after the procedure. Methods: A total of 105 patients underwent uncomplicated URSL for ureteric stones were prospectively randomized into three groups: group 1 (34 patients) with DJ-US, group 2 (35 patients) with DJ-US on extraction string, and group 3 (36 patients) with no DJ-US after the procedure. The outcomes measured were; postoperative Visual Analog Score (VAS) for flank pain and dysuria score, urgency, frequency, suprapubic pain, hematuria, analgesia requirement, operative time, re-hospitalization, and return to normal physical activity.Results: Mean operative time was significantly longer in groups 1 and 2 compared to group 3 [mean time ± SD, 22.2 ± 9.1 min, 20.2 ± 6 min, 15.1 ± 7.1 min respectively, p<0.0001]. The results of the VAS for flank pain and dysuria scores, urgency, frequency, hematuria, and suprapubic pain showed a significant difference at all time points of follow-up, with significantly higher in groups 1 and 2 compared to group 3. Further analysis showed that measured outcomes, and analgesia need for groups 1 and 2 were similar, at all time points except at week 1 and 1 month where group 2 patient’s had less symptoms.Conclusion: DJ-US placement appear to be unnecessary in procedures considered uncomplicated by operating urologists during surgery. The advantages of DJ-US with extraction string over DJ-US only include earlier and easy removal with earlier relief of symptoms, and less analgesia requirements.

scholarly journals Evaluation of Neutrophil Gelatinase-Associated Lipocalin and Cystatin C in Early Diagnosis of Chronic Kidney Disease in the Absence of the Gold Standard

2020 ◽  
Vol 9 ◽  
pp. 1698
Author(s):  
Fatemeh Masaebi ◽  
Mehdi Azizmohammad Looha ◽  
Zhuoyu Wang ◽  
Elaheh Zarean ◽  
Maliheh Nasiri ◽  
...  
Keyword(s):  
Early Detection ◽  
Kidney Function ◽  
Cystatin C ◽  
Roc Curve ◽  
Gold Standard ◽  
Predictive Power ◽  
Latent Class ◽  
Added Value ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Neutrophil Gelatinase

Background: Glomerular filtration rate (GFR) is considered as a gold standard of kidney function. However, using GFR as the gold standard is not common in clinical practice, because its direct measurement is usually expensive, cumbersome, and invasive. In the present study, we assessed the predictive power of two other biomarkers, Cystatin-C (Cys-C) and Neutrophil Gelatinase-Associated Lipocalin (NGAL) for early detection of chronic kidney diseases (CKD) in the absence of a gold standard. Materials and Methods: In this study, 72 patients who referred to the Shohadaye Tajrish Hospital of Tehran, Iran, for measuring their kidney function were studied. The ELISA method was utilized for measuring plasma NGAL (PNGAL) and serum Cys-C (SCys-C). The Bayesian latent class modeling approach was applied to asses the predictive power of these biomarkers. Results: While both the biomarkers had rather high sensitivities (PNGAL=91%, SCys-C= 89%), the specificity of SCys-C biomarker was very lower than the one of PNGAL (SCys-C=56%, PNGAL=94%). The estimated area under the receiver operating characteristic (ROC) curve for SCys-C as the single biomarker for the diagnosis of CKD was about 0.76, while a similar estimate for PNGAL was 0.93. The added value of PNGAL to SCys-C for the diagnosis of CKD in terms of the ROC curve was about 0.19, while the added value of SCys-C to PNGAL was less than 0.02. Conclusion: In general, our findings suggest that PNGAL can be utilized as a single reliable biomarker for early detection of CKD. In addition, results showed that when a perfect gold standard is not available, Bayesian approaches to latent class models could lead to more precise sensitivity and specificity estimates of imperfect tests. [GMJ.2020;9:e1698] 

scholarly journals Alkalmas-e a vizelet neutrofil gelatináz asszociálta lipokalin meghatározása a rejekció előrejelzésére veseátültetés után?

2015 ◽  
Vol 156 (48) ◽  
pp. 1956-1959
Author(s):  
Gábor Telkes ◽  
Gábor Dallos ◽  
Marina Varga
Keyword(s):  
Negative Impact ◽  
Graft Function ◽  
Kidney Injury ◽  
Delayed Graft Function ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Kidney Recipients ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Neutrophil Gelatinase

Introduction: Delayed graft function and acute rejection have negative impact on graft survival. Aim: To asses the predictive value of urinary neutrophil gelatinase-associated lipocalin, which has been found to be a promising biomarker for the diagnosis of acute kidney injury. Method: In this prospective study urinary neutrophil gelatinase-associated lipocalin levels of 27 kidney recipients were measured. Results: Patients were grouped as follows: group 1, no complication; group 2, rejection; group 3, delayed graft function requiring dialysis; group 4, rejection plus delayed graft function. There were no significant differences between groups 1 and 2, and between groups 3 and 4. Patients in groups 3 and 4 had significantly higher urinary neutrophil gelatinase-associated lipocalin levels as compared to those in groups 3 and 4. There was a paralIel change in urinary neutrophil gelatinase-associated lipocalin levels in groups 1 and 2. Conclusions: In these patients urinary neutrophil gelatinase-associated lipocalin levels failed to provide useful information in both cases of normal and impaired function. Orv. Hetil., 2015, 156(48), 1956–1959.

Neutrophil Gelatinase-Associated Lipocaline (NGAL) an early predictive biomarker than Urea in contrast induced Nephropathy

2016 ◽  
Vol 5 (03) ◽  
pp. 4851
Author(s):  
Shobha M.* ◽  
Shanthi Naidu ◽  
Vijayaraghavan R. ◽  
Mujahid M. ◽  
Senthil Kumar S.
Keyword(s):  
Kidney Injury ◽  
Predictive Biomarker ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Iodinated Contrast ◽  
Before And After ◽  
Group 3 ◽  
Group 2 ◽  
Radiological Procedures ◽  
Neutrophil Gelatinase ◽  
Hospital Acquired

Coronary angiography and percutaneous coronary interventions depend on iodinated and non- iodinated contrast media and consequently pose the risk of contrast induced acute kidney injury (AKI). This is an important complication that accounts for significant number of cases of hospital acquired renal failure, with adverse effects on prognosis and health care. Aim of this study is to evaluate Neutrophil Gelatinase- Associated Lipocalin (NGAL) as an early biomarker in AKI than that of urea. 30 animals were randomly divided into 10 different cages having 3 animals in each cage. Further cages were randomly divided into 5 groups (6 animals in a group). Dose of 0.5ml of iohexol was intraperitonealy injected into animals. Blood samples were collected by bleeding retroorbital plexus before and after inducing contrast and centrifuged serum was stored at -200c for further analysis. Increased levels of NGAL was indicated in group 2 (6 hours) and remained elevated in group 3 (12 hours) when compared to that of baseline levels before contrast. The levels of urea were increased in group 1 (3 hours) and group 3 (12 hours) after contrast when compared to level of baseline before contrast. Elevated levels of NGAL among the groups was statistically significant p<0.05. The increased level of urea at the end of 3 hours is probably due to dehydration after contrast induction. Estimation of serum NGAL after contrast induced radiological procedures especially after coronary angiogram may help to detect early kidney injury, so that preventive measures can be adopted to decrease the damage caused by the contrast induction.

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