Human Milk Oligosaccharide 2′-Fucosyllactose Induces Neuroprotection from Intracerebral Hemorrhage Stroke

Intracerebral hemorrhage (ICH) occurs when brain blood vessels rupture, causing inflammation and cell death. 2-Fucosyllactose (2FL), a human milk oligosaccharide, has potent antiapoptotic and anti-inflammatory effects. The purpose of this study was to examine the protective effect of 2FL in cellular and rodent models of ICH. Hemin was added to a primary rat cortical neuronal and BV2 microglia coculture to simulate ICH in vitro. IBA1 and MAP2 immunoreactivities were used to determine inflammation and neuronal survival. Hemin significantly increased IBA1, while it reduced MAP2 immunoreactivity. 2FL significantly antagonized both responses. The protective effect of 2FL was next examined in a rat ICH model. Intracerebral administration of type VII collagenase reduced open-field locomotor activity. Early post-treatment with 2FL significantly improved locomotor activity. Brain tissues were collected for immunohistochemistry and qRT-PCR analysis. 2FL reduced IBA1 and CD4 immunoreactivity in the lesioned striatum. 2FL downregulated the expression of ER stress markers (PERK and CHOP), while it upregulated M2 macrophage markers (CD206 and TGFβ) in the lesioned brain. Taken together, our data support that 2FL has a neuroprotective effect against ICH through the inhibition of neuroinflammation and ER stress. 2FL may have clinical implications for the treatment of ICH.

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Correction to: Gold standard for nutrition: a review of human milk oligosaccharide and its effects on infant gut microbiota

Microbial Cell Factories ◽

10.1186/s12934-021-01612-4 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Shunhao Zhang ◽

Tianle Li ◽

Jing Xie ◽

Demao Zhang ◽

Caixia Pi ◽

...

Keyword(s):

Gut Microbiota ◽

Human Milk ◽

Gold Standard ◽

Milk Oligosaccharide ◽

Human Milk Oligosaccharide

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Human Milk Oligosaccharide Profile Variation Throughout Postpartum in Healthy Women in a Brazilian Cohort

Nutrients ◽

10.3390/nu12030790 ◽

2020 ◽

Vol 12 (3) ◽

pp. 790 ◽

Cited By ~ 3

Author(s):

Ana Lorena Ferreira ◽

Ronaldo Alves ◽

Amanda Figueiredo ◽

Nadya Alves-Santos ◽

Nathalia Freitas-Costa ◽

...

Keyword(s):

Human Milk ◽

Rank Correlation ◽

Postpartum Visit ◽

Maternal Characteristics ◽

Milk Oligosaccharide ◽

Healthy Women ◽

Human Milk Oligosaccharide ◽

Post Hoc ◽

Brazilian Cohort ◽

Non Negative Matrix Factorization

Human milk oligosaccharide (HMO) composition varies throughout lactation and can be influenced by maternal characteristics. This study describes HMO variation up to three months postpartum and explores the influences of maternal sociodemographic and anthropometric characteristics in a Brazilian prospective cohort. We followed 101 subjects from 28–35 gestational weeks (baseline) and throughout lactation at 2–8 (visit 1), 28–50 (visit 2) and 88–119 days postpartum (visit 3). Milk samples were collected at visits 1, 2 and 3, and 19 HMOs were quantified usinghigh-performance liquid chromatography with fluorescence detection (HPLC-FL). Friedman post-hoc test, Spearman rank correlation for maternal characteristics and HMOs and non-negative matrix factorization (NMF) were used to define the HMO profile. Most women were secretors (89.1%) and presented high proportion of 2′-fucosyllactose (2′FL) at all three sample times, while lacto-N-tetraose (LNT, 2–8 days) and lacto-N-fucopentaose II (LNFPII, 28–50 and 88–119 days) were the most abundant HMOs in non-secretor women. Over the course of lactation, total HMO weight concentrations (g/L) decreased, but total HMO molar concentrations (mmol/L) increased, highlighting differential changes in HMO composition over time. In addition, maternal pre-pregnancy body mass index (BMI) and parity influence the HMO composition in healthy women in this Brazilian cohort.

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