scholarly journals Formulation of New Media from Dairy and Brewery Wastes for a Sustainable Production of DHA-Rich Oil by Aurantiochytrium mangrovei

Marine Drugs ◽  
2021 ◽  
Vol 20 (1) ◽  
pp. 39
Author(s):  
Giovanni L. Russo ◽  
Antonio L. Langellotti ◽  
Vito Verardo ◽  
Beatriz Martín-García ◽  
Prospero Di Pierro ◽  
...  
Keyword(s):  
New Media ◽  
Sustainable Growth ◽  
Growth Media ◽  
Mozzarella Cheese ◽  
Omega 3 ◽  
Bioactive Lipids ◽  
Biotechnological Processes ◽  
Brewery Yeast ◽  
Disposal Cost ◽  
Cheese Production

Mozzarella stretching water (MSW) is a dairy effluent generated from mozzarella cheese production that does not have a real use and is destined to disposal, causing environmental problems and representing a high disposal cost for dairy producers. Spent brewery yeast (SBY) is another promising food waste produced after brewery manufacturing that could be recycled in new biotechnological processes. Aurantiochytrium mangrovei is an aquatic protist known as producer of bioactive lipids such as omega 3 long chain polyunsaturated fatty acids (ω3 LC-PUFA), in particular docosahexaenoic acid (DHA). In this work MSW and SBY have been used to formulate new sustainable growth media for A. mangrovei cultivation and production of DHA in an attempt to valorize these effluents. MSW required an enzymatic hydrolysis to enhance the biomass production. The new media obtained from hydrolysed MSW was also optimized using response surface methodologies, obtaining 10.14 g L−1 of biomass in optimized medium, with a DHA content of 1.21 g L−1.

scholarly journals Microalgae as Sustainable Bio-Factories of Healthy Lipids: Evaluating Fatty Acid Content and Antioxidant Activity

Marine Drugs ◽  
2021 ◽  
Vol 19 (7) ◽  
pp. 357
Author(s):  
Tiago A. Conde ◽  
Bruna F. Neves ◽  
Daniela Couto ◽  
Tânia Melo ◽  
Bruno Neves ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Fatty Acid Content ◽  
Scenedesmus Obliquus ◽  
Food Sources ◽  
Gas Chromatography Mass Spectrometry ◽  
Nannochloropsis Oceanica ◽  
Omega 3 ◽  
Bioactive Lipids ◽  
Food Ingredients

The demand for sustainable and environmentally friendly food sources and food ingredients is increasing, and microalgae are promoted as a sustainable source of essential and bioactive lipids, with high levels of omega-3 fatty acids (ω-3 FA), comparable to those of fish. However, most FA screening studies on algae are scattered or use different methodologies, preventing a true comparison of its content between microalgae. In this work, we used gas-chromatography mass-spectrometry (GC-MS) to characterize the FA profile of seven different commercial microalgae with biotechnological applications (Chlorella vulgaris, Chlorococcum amblystomatis, Scenedesmus obliquus, Tetraselmis chui, Phaeodactylum tricornutum, Spirulina sp., and Nannochloropsis oceanica). Screening for antioxidant activity was also performed to understand the relationship between FA profile and bioactivity. Microalgae exhibited specific FA profiles with a different composition, namely in the ω-3 FA profile, but with species of the same phylum showing similar tendencies. The different lipid extracts showed similar antioxidant activities, but with a low activity of the extracts of Nannochloropsis oceanica. Overall, this study provides a direct comparison of FA profiles between microalgae species, supporting the role of these species as alternative, sustainable, and healthy sources of essential lipids.

scholarly journals The Cheese Production Facility Microbiome Exhibits Temporal and Spatial Variability

2021 ◽  
Vol 12 ◽  
Author(s):  
Jared Johnson ◽  
Chris Curtin ◽  
Joy Waite-Cusic
Keyword(s):  
Product Quality ◽  
Cheddar Cheese ◽  
Starter Cultures ◽  
Growth Media ◽  
Food Contact ◽  
Food Contact Surfaces ◽  
Repeated Sampling ◽  
Conveyor Belts ◽  
Contact Surfaces ◽  
Cheese Production

A primary goal of modern cheese manufacturing is consistent product quality. One aspect of product quality that remains poorly understood is the variability of microbial subpopulations due to temporal or facility changes within cheese production environments. Therefore, our aim was to quantify this variability by measuring day-day and facility-facility changes in the cheese facility microbiome. In-process product (i.e., milk and cheese) and food-contact surfaces were sampled over the course of three production days at three cheese manufacturing facilities. Microbial communities were characterized using 16S rRNA metabarcoding and by plating on selective growth media. Each facility produced near-identical Cheddar cheese recipes on near-identical processing equipment during the time of sampling. Each facility also used a common pool of Lactococcus starter cultures which were rotated daily as groups of 4–5 strains and selected independently at each facility. Diversity analysis revealed significant facility-facility and day-day differences at each sample location. Facility differences were greatest on the food contact surfaces (i.e., draining-matting conveyor belts), explaining between 25 and 41% of the variance. Conversely, daily differences within each facility explained a greater proportion of the variance in the milk (20% vs. 12%) and cheese (29% vs. 20%). Further investigation into the sources of these differences revealed the involvement of several industrially relevant bacteria, including lactobacilli, which play a central role in flavor and texture development during Cheddar cheese ripening. Additionally, Streptococcus was found to contribute notably to differences observed in milk samples, whereas Acinetobacter, Streptococcus, Lactococcus, Exiguobacterium, and Enterobacteriaceae contributed notably to differences on the food contact surfaces. Facility differences in the cheese were overwhelmingly attributed to the rotation of Lactococcus starter cultures, thus highlighting circumstances where daily microbial shifts could be misinterpreted and emphasizing the importance of repeated sampling over time. The outcomes of this work highlight the complexity of the cheese facility microbiome and demonstrate daily and facility-facility microbial variations which might impact cheese product quality.

scholarly journals Comparison of Three Deoxidation Agents for Ozonated Broths Used in Anaerobic Biotechnological Processes

Processes ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 65
Author(s):  
Ewelina Pawlikowska ◽  
Jaroslaw Domanski ◽  
Piotr Dziugan ◽  
Joanna Berlowska ◽  
Weronika Cieciura-Wloch ◽  
...  
Keyword(s):  
Anaerobic Fermentation ◽  
Process Conditions ◽  
Growth Media ◽  
Nutrient Supplementation ◽  
Yeast Biomass ◽  
Residual Oxygen ◽  
The Media ◽  
Biotechnological Processes ◽  
The Subject ◽  
High Concentrations

Anaerobic fermentation of organic compounds is used in many biotechnological processes and has been the subject of much research. A variety of process conditions and different growth media can be used to obtain microbial metabolites. The media must be free from contamination before fermentation. Sterilization is most often achieved by applying heat or other treatments, such as ozonation. Sterilization of liquid media using ozone can be very beneficial, but this method introduces high concentrations of residual oxygen, which inhibit anaerobic processes. Deoxidation is therefore necessary to remove the oxygen from ozonated broths. This study evaluates the effectiveness of three deoxidation agents for two kinds of fermentation media based on malt or molasses: ultrasound, iron(II) sulfate, and Metschnikowia sp. yeast. The time needed for deoxidation varied, depending on the kind of broth and the deoxidation agent. In general, the dynamics of oxygen removal were faster in malt broth. A comparative analysis showed that yeast biomass was the most effective agent, achieving deoxidation in the shortest time. Moreover, the fully deoxidated broth was supplemented with yeast biomass, which is rich in biogenic substrates, expressed as a protein content of 0.13–0.73 g/L. Application of Metschnikowia sp. may therefore be considered as an effective strategy for simultaneous deoxidation and nutrient supplementation of broths used in anaerobic biotechnological processes.

scholarly journals Imbalance Between Omega-6- and Omega-3-Derived Bioactive Lipids in Arthritis in Older Adults

2020 ◽  
Author(s):  
Roxana Coras ◽  
Brian Pedersen ◽  
Rekha Narasimhan ◽  
Anahy Brandy ◽  
Lourdes Mateo ◽  
...  
Keyword(s):  
Older Adults ◽  
Disease Activity Score ◽  
Eicosatrienoic Acid ◽  
Mass Spectrometry Data ◽  
Omega 3 ◽  
Bioactive Lipids ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Omega 6 ◽  
Elderly Onset

Abstract Elderly-onset rheumatoid arthritis (EORA) and polymyalgia rheumatica (PMR) are common rheumatic diseases in older adults. Oxylipins are bioactive lipids derived from omega-6 (n-6) and omega-3 (n-3) polyunsaturated fatty acids (PUFAs) that serve as activators or suppressors of systemic inflammation. We hypothesized that arthritis symptoms in older adults were related to oxylipin-related perturbations. Arthritis in older adults (ARTIEL) is an observational prospective cohort with 64 patients older than 60 years of age with newly diagnosed arthritis. Patients’ blood samples at baseline and 3 months posttreatment were compared with 18 controls. A thorough clinical examination was conducted. Serum oxylipins were determined by mass spectrometry. Data processing and statistical analysis were performed in R. Forty-four patients were diagnosed with EORA and 20 with PMR. At diagnosis, EORA patients had a mean DAS28CRP (Disease Activity Score 28 using C-reactive protein) of 5.77 (SD 1.02). One hundred percent of PMR patients reported shoulder pain and 90% reported pelvic pain. Several n-6- and n-3-derived oxylipin species were significantly different between controls and arthritis patients. The ratio of n-3/n-6 PUFA was significantly downregulated in EORA but not in PMR patients as compared to controls. The top two candidates as biomarkers for differentiating PMR from EORA were 4-HDoHE, a hydroxydocosahexaenoic acid, and 8,15-dihydroxy-eicosatrienoic acid (8,15-diHETE). The levels of n-3-derived anti-inflammatory species increased in EORA after treatment. These results suggest that certain oxylipins may be key effectors in arthrtis in older adults and that the imbalance between n-6- and n-3-derived oxylipins might be related to pathobiology in this population.

Consumers’ willingness to pay and drivers of motivation to consume omega-3 enriched mozzarella cheese

2016 ◽  
Vol 118 (10) ◽  
pp. 2404-2419 ◽  
Author(s):  
Riccardo Vecchio ◽  
Alessia Lombardi ◽  
Luigi Cembalo ◽  
Francesco Caracciolo ◽  
Gianni Cicia ◽  
...  
Keyword(s):  
Willingness To Pay ◽  
Self Efficacy ◽  
Structural Equation ◽  
Outcome Expectations ◽  
Outcome Expectation ◽  
Specific Information ◽  
Mozzarella Cheese ◽  
Omega 3 ◽  
Content Type ◽  
Self Identity

Purpose Consumer interest and willingness to pay (WTP) for omega-3 enriched water buffalo mozzarella cheese are evaluated through an in-store experimental auction. The purpose of this paper is to estimate individual WTP for enriched mozzarella cheese and related it to self-efficacy beliefs and outcome expectations of omega-3 consumption, following regulatory focus theory. Design/methodology/approach Data were collected in May-June 2015 from a sample of 150 consumers in Southern Italy. A structural equation modelling procedure was implemented. Findings The results show a significant role is played by prevention outcome expectations on consumer behaviour. While promotion outcome expectation constructs proved non-significant, self-identity correlates with prevention outcome expectations. Research limitations/implications There are several limitations that the authors are aware of regarding this study. First of all, since the authors rely on self-reported measures, optimistic bias might have affected participants’ responses (Weinstein, 1980). Second, results may be influenced by the choice of the specific information provided to consumers for the analysis; different claims and different information framings should be tested (LeBoeuf and Shafir, 2003). Practical implications Implications stemming from the results encourage the promotion of omega-3 enriched mozzarella cheese based on stimulating outcome expectations, bearing in mind that individual motivations should be enhanced by self-identity beliefs. Originality/value Although the combined role of self-efficacy and outcome expectations on personal intention to adopt healthy behaviour has already been demonstrated (Keller, 2006; Tudoran et al., 2012), to the best of the knowledge no previous study relates individual behaviour to an intention measured as a WTP for an actual product. In addition, current study has applied a non-hypothetical BDM (from Becker et al., 1964) auction in-store experiment.

scholarly journals Mechanization of fibrous-structured cheese production process I. Rheological properities of Mozzarella cheese curds.

1990 ◽  
pp. 621-627 ◽  
Author(s):  
Tadashi IZUTSU ◽  
Masayuki AZUMA ◽  
Toshiaki KIMURA ◽  
Masayoshi FUKUSHIMA
Keyword(s):  
Production Process ◽  
Mozzarella Cheese ◽  
Cheese Production

Potential role of bioactive lipids in rheumatoid arthritis

2021 ◽  
Vol 27 ◽  
Author(s):  
Wheeler Torres ◽  
Mervin Chávez-Castillo ◽  
José L. Peréz-Vicuña ◽  
Rubén Carrasquero ◽  
María P. Díaz ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Potential Role ◽  
The Body ◽  
Omega 3 ◽  
Bioactive Lipids ◽  
Dietary Interventions ◽  
Complex Disorder ◽  
Inflammatory Autoimmune Disease

: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease, which involves a pathological inflammatory response against articular cartilage in multiple joints throughout the body. It is a complex disorder associated with comorbidities such as depression, lymphoma, osteoporosis and cardiovascular disease (CVD), which significantly deteriorate patients’ quality of life and prognosis. This has ignited a large initiative to elucidate the physiopathology of RA, aiming to identify new therapeutic targets and approaches in its multidisciplinary management. Recently, various lipid bioactive products have been proposed to have an essential role in this process; including eicosanoids, specialized pro-resolving mediators, phospholipids/sphingolipids, and endocannabinoids. Dietary interventions using omega-3 polyunsaturated fatty acids or treatment with synthetic endocannabinoids agonists have been shown to significantly ameliorate RA symptoms. Indeed, the modulation of lipid metabolism may be crucial in the pathophysiology and treatment of autoimmune diseases.

Quantitative profiling of inflammatory and pro-resolving lipid mediators in human adolescents and mouse plasma using UHPLC-MS/MS

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ivan Hartling ◽  
Alessio Cremonesi ◽  
Ester Osuna ◽  
Phing-How Lou ◽  
Eliana Lucchinetti ◽  
...  
Keyword(s):  
Human Plasma ◽  
Lipid Mediators ◽  
Lipid Mediator ◽  
Prostaglandin D2 ◽  
Reference Ranges ◽  
Good Precision ◽  
Plasma Samples ◽  
Omega 3 ◽  
Bioactive Lipids ◽  
Blood Draw

Abstract Objectives Lipid mediators are bioactive lipids which help regulate inflammation. We aimed to develop an ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to quantify 58 pro-inflammatory and pro-resolving lipid mediators in plasma, determine preliminary reference ranges for adolescents, and investigate how total parenteral nutrition (TPN) containing omega-3 polyunsaturated fatty acid (n-3 PUFA) or n-6 PUFA based lipid emulsions influence lipid mediator concentrations in plasma. Methods Lipid mediators were extracted from plasma using SPE and measured using UHPLC-MS/MS. EDTA plasma was collected from healthy adolescents between 13 and 17 years of age to determine preliminary reference ranges and from mice given intravenous TPN for seven days containing either an n-3 PUFA or n-6 PUFA based lipid emulsion. Results We successfully quantified 43 lipid mediators in human plasma with good precision and recovery including several leukotrienes, prostaglandins, resolvins, protectins, maresins, and lipoxins. We found that the addition of methanol to human plasma after blood separation reduces post blood draw increases in 12-hydroxyeicosatetraenoic acid (12-HETE), 12-hydroxyeicosapentaenoic acid (12-HEPE), 12S-hydroxyeicosatrienoic acid (12S-HETrE), 14-hydroxydocosahexaenoic acid (14-HDHA) and thromboxane B2 (TXB2). Compared to the n-6 PUFA based TPN, the n-3 PUFA based TPN increased specialized pro-resolving mediators such as maresin 1 (MaR1), MaR2, protectin D1 (PD1), PDX, and resolvin D5 (RvD5), and decreased inflammatory lipid mediators such as leukotriene B4 (LTB4) and prostaglandin D2 (PGD2). Conclusions Our method provides an accurate and sensitive quantification of 58 lipid mediators from plasma samples, which we used to establish a preliminary reference range for lipid mediators in plasma samples of adolescents; and to show that n-3 PUFA, compared to n-6 PUFA rich TPN, leads to a less inflammatory lipid mediator profile in mice.

Sign in / Sign up

Export Citation Format

Share Document