scholarly journals Using HPLC–DAD and GC–MS Analysis Isolation and Identification of Anticandida Compounds from Gui Zhen Cao Herbs (Genus Bidens): An Important Chinese Medicinal Formulation

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5820
Author(s):  
Kulsoom Zahara ◽  
Yamin Bibi ◽  
Saadia Masood ◽  
Sobia Nisa ◽  
Abdul Qayyum ◽  
...  
Keyword(s):  
High Performance ◽  
Gel Chromatography ◽  
Bidens Pilosa ◽  
Isolation And Identification ◽  
Herbal Formulation ◽  
Ms Analysis ◽  
Array Detection ◽  
Medicinal Formulation ◽  
Silica Gel Chromatography

Gui Zhen Cao is an herbal formulation that has been documented in Chinese traditional medicine as a remedy for diarrhea, dysentery, inflammation, and toxicity. The sources of this formulation (Bidens pilosa L., Bidens biternata (Lour.) Merr. & Sherff, Bidens bipinnata L.) are also listed in ethnomedicinal reports all over the world. In this study, all these plants are tested for in vitro anticandida activity. A quantitative evaluation of the phytochemicals in all these plants indicated that their vegetative parts are rich in tannins, saponins, oxalates, cyanogenic glycoside and lipids; moreover, the roots have high percentages of alkaloids, flavonoids, and phenols. The results indicated significant anticandida activity, especially for the hexane extract of B. bipinnata leaves which inhibited C. albicans (42.54%), C. glabrata (46.98%), C. tropicalis (50.89%), C. krusei (40.56%), and C. orthopsilosis (50.24%). The extract was subjected to silica gel chromatography and 220 fractions were obtained. Purification by High Performance Liquid Chromatography with Diode-Array Detection (HPLC–DAD) and Gas Chromatography tandem Mass Spectrometry (GC-MS/MS) analysis led to the identification of two anticandida compounds: dehydroabietic and linoleic acid having an inhibition of 85 and 92%, respectively.

scholarly journals Inhibitory effect of the glucosinolate–myrosinase system on Phytophthora cinnamomi and Pythium spiculum

2019 ◽  
Vol 55 (No. 2) ◽  
pp. 93-101 ◽  
Author(s):  
Francisco Teodoro Arroyo Cordero ◽  
Rocío Rodríguez-Arcos ◽  
Ana Jiménez-Araujo ◽  
Rafael Guillén-Bejarano ◽  
María José Basallote ◽  
...  
Keyword(s):  
Mycelial Growth ◽  
High Performance ◽  
Phytophthora Cinnamomi ◽  
Inhibitory Effect ◽  
Reversed Phase ◽  
Field Application ◽  
Additional Information ◽  
Array Detection ◽  
Important Additional Information

Glucosinolate extracts from sprouts of common Brassica nigra, B. juncea cv. Scala, B. carinata cv. Eleven, and Sinapis alba cv. Ludique were analysed by reversed phase high-performance liquid chromatography-diode array detection-mass spectrometry. The effect of the glucosinolate–myrosinase system on in vitro mycelial growth of Phytophthora cinnamomi Rands and Pythium spiculum B. Paul was assessed. Likewise, sinigrin and sinalbin monohydrate commercial standards were also tested. The extracts from B. carinata, which contained 159 mmol/g plant DW equivalent (85% sinigrin, 5% gluconapin, and 3% glucotropaeolin), were the most effective against Phytophthora and Pythium isolates used in this study. However, the extract from S. alba, which contained 1 180 mmol/g (100% sinalbin), did not inhibit the mycelial growth of the isolates tested. The use of the glucosinolate-myrosinase system provides important additional information to advance in the implementation of field application of brassicaceous amendments for the control of soil-borne pathogens.

scholarly journals Isolation and characterization of ardicrenin from Ardisia crenata Sims

2009 ◽  
Vol 55 (No. 7) ◽  
pp. 305-310 ◽  
Author(s):  
Ma Yuan ◽  
Pu Shangrao ◽  
Cheng Qingsu ◽  
Ma Mingdong
Keyword(s):  
High Performance ◽  
Amorphous Powder ◽  
Gel Chromatography ◽  
Macroporous Resin ◽  
Resin Column ◽  
Isolation And Characterization ◽  
Natural Reserve ◽  
Silica Gel Chromatography ◽  
Counter Current

A new, effective and economical method to extract ardicrenin from <I>Ardisia crenata</I> Sims collected in the Wolong natural reserve, Sichuan, China, is established. <I>Ardisia crenata</I> Sims powder is counter-current extracted with 80% methanol reflux, decompressively enriched and centrifuged to defat. Supernatant is applied to macroporous resin column (AB-8) with 80% methanol, ardicrenin is isolated by silica gel chromatography with dichlormethane-acetoacetate-methanol (4:1.5:1) washing, and recrystallized in methanol. The final product which proved to be ardicrenin by analytic procedure including Furier transform infrared (FTIR) and ultraviolet spectrum (UV), mass spectroscopy (MS), nuclear magnetic resonance (NMR) and high performance liquid chromatography (HPLC) is white amorphous powder with yield of 1.59 ± 0.02%.

Latent Collagenase Production by Cells Derived from Nasal Polyps in Culture

1994 ◽  
Vol 8 (5) ◽  
pp. 241-246 ◽  
Author(s):  
Tsutomu Matsuzaki ◽  
Kazunori Itoh ◽  
Shoko Katahira ◽  
Masaru Ohyama ◽  
Katsunori Fukuda
Keyword(s):  
High Performance ◽  
Chronic Sinusitis ◽  
Interleukin 1 ◽  
Gel Chromatography ◽  
Ammonium Sulfate Precipitation ◽  
Inflammatory Lesions ◽  
Nasal Secretions ◽  
Connective Tissue Metabolism

We investigated the interactions between nasal polyp-derived fibroblast-like cells (NPFCs) and nasal secretions to clarify the role of connective tissue metabolism in nasal and paranasal pathology, especially in chronic sinusitis. In response to stimulation by human interleukin 1 (IL-1), NPFCs produced latent collagenase. Collagenase production was also stimulated by nasal secretions from patients with chronic sinusitis in vitro, but not by nasal secretions from nasal allergic patients. A soluble factor was isolated from the nasal secretions of patients with chronic sinusitis by ammonium sulfate precipitation and Sephadex G-75 gel chromatography. High performance liquid chromatography indicated that the molecular weight of the active fraction was approximately 20,000 daltons. This factor affected NPFCs, but did not affect the endothelial cells derived from nasal mucosa in culture. Collagenase production by NPFCs was considerably inhibited by anti-IL-1 antibody. These results indicate that IL-1 is one major factor to prolong the nasal and paranasal inflammatory lesions.

scholarly journals Assessment of environmental condition and drying process of the plants on the concentration of alkaloids and cytotoxicity of traditional Ayahuasca Tea

2021 ◽  
Vol 10 (2) ◽  
pp. 075-089
Author(s):  
Miranda Ordilena Ferreira de ◽  
Almeida Marcílio de ◽  
Sousa Ilza Maria de Oliveira ◽  
Ruiz Ana Lúcia Tasca Gois ◽  
Costa José Luiz da ◽  
...  
Keyword(s):  
High Performance ◽  
Colorimetric Assay ◽  
Human Keratinocytes ◽  
In Vitro Cytotoxicity ◽  
Drying Method ◽  
Drying Process ◽  
Forced Circulation ◽  
Different Temperatures ◽  
Array Detection

Introduction: Ayahuasca is a traditional psychoactive tea of Amazonian indigenous, used medicinal and spiritual purposes. Wide variation in the concentration of N,N-dimethyltryptamine (DMT), Harmaline (HRL), Harmine (HRM) and Tetrahydroharmine (THH) alkaloids in Ayahuasca has been reported worldwide. Objective: To evaluate the causes of variations in alkaloids concentrations of Ayahuasca prepared with fresh and dehydrated plants from different environments and determine the best drying method to plants according to alkaloids content and cytotoxicity of Ayahuasca tea. Material and methods: The environment interference on the alkaloids of the two species was evaluated in samples of Ayahuasca tea prepared with fresh plants. The most suitable drying process to the two species was evaluated in sample Ayahuasca tea prepared with plants submitted to drying under the sun conditions and five different temperatures in forced circulation oven. The concentration of the alkaloids determined by high performance liquid chromatography with UV-vis detector with diode array detection (HPLC-DAD). The in vitro cytotoxicity of Ayahuasca was evaluated in human keratinocytes cells (HaCaT) by colorimetric assay. Results: Environmental characteristics, preparation process and temperature of plants drying interfered on DMT, HRL, HRM and THH concentrations of Ayahuasca. No effect cytotoxicity was detected with relationship to psychoactive alkaloids in samples of Ayahuasca tea prepared with fresh or dried plants. Conclusion: Concentration of DMT, HRL, HRM and THH alkaloids in Ayahuasca are influenced by plants environmental. The most suitable drying process was obtained in forced circulation oven at 43 and 45°C to P. viridis leaves and B. caapi stems respectively. The Ayahuasca prepared with fresh or dry plants no showed cytotoxicity in human keratinocytes cells.

scholarly journals Phenolic Extract fromMoringa oleiferaLeaves Inhibits Key Enzymes Linked to Erectile Dysfunction and Oxidative Stress in Rats’ Penile Tissues

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Ganiyu Oboh ◽  
Adedayo O. Ademiluyi ◽  
Ayokunle O. Ademosun ◽  
Tosin A. Olasehinde ◽  
Sunday I. Oyeleye ◽  
...  
Keyword(s):  
Erectile Dysfunction ◽  
High Performance ◽  
Antioxidant Properties ◽  
Tissue Homogenate ◽  
Phenolic Contents ◽  
Phenolic Constituents ◽  
Array Detection ◽  
Phenolic Extract ◽  
Dose Dependent

This study was designed to determine the antioxidant properties and inhibitory effects of extract fromMoringa oleiferaleaves on angiotensin-I-converting enzyme (ACE) and arginase activitiesin vitro. The extract was prepared and phenolic (total phenols and flavonoid) contents, radical (nitric oxide (NO), hydroxyl (OH)) scavenging abilities, and Fe2+-chelating ability were assessed. Characterization of the phenolic constituents was done via high performance liquid chromatography-diode array detection (HPLC-DAD) analysis. Furthermore, the effects of the extract on Fe2+-induced MDA production in rats’ penile tissue homogenate as well as its action on ACE and arginase activities were also determined. The extract scavengedNO∗,OH∗, chelated Fe2+, and inhibited MDA production in a dose-dependent pattern with IC50values of 1.36, 0.52, and 0.38 mg/mL and 194.23 µg/mL, respectively. Gallic acid, chlorogenic acid, quercetin, and kaempferol were the most abundant phenolic compounds identified in the leaf extract. The extract also inhibited ACE and arginase activities in a dose-dependent pattern and their IC50values were 303.03 and 159.59 µg/mL, respectively. The phenolic contents, inhibition of ACE, arginase, and Fe2+-induced MDA production, and radical (OH∗,NO∗) scavenging and Fe2+-chelating abilities could be some of the possible mechanisms by whichM. oleiferaleaves could be used in the treatment and/or management of erectile dysfunction.

scholarly journals Dalbergia ecastaphyllum leaf extracts: in vitro inhibitory potential against enzymes related to metabolic syndrome, inflammation and neurodegenerative diseases

2019 ◽  
Vol 41 ◽  
pp. e46622
Author(s):  
Daniel Vieira de Morais ◽  
Manuela Maria Moreira ◽  
Fabiane de Lima Silva ◽  
Maria Angélica Pereira de Carvalho Costa ◽  
Cristina Delerue-Mato ◽  
...  
Keyword(s):  
Hemolytic Activity ◽  
Inhibitory Activity ◽  
High Performance ◽  
Leaf Extracts ◽  
Photodiode Array Detection ◽  
Photodiode Array ◽  
Array Detection ◽  
Inhibitory Activities ◽  
Inhibitory Potential

For the first time, the anti-hemolytic activity and the enzyme inhibitory activities of Dalbergia ecastaphyllum leaves extracts were tested against α-amylase, α-glucosidase, lipase, acetylcholinesterase, butyrylcholinesterase, tyrosinase and hyaluronidase. The phenolic profile of the obtained extracts was also investigated by high-performance liquid chromatography with photodiode array detection (HPLC-PAD). The extracts showed inhibitory activity against all enzymes evaluated, with the highest inhibitory activity reported for the enzyme hyaluronidase (28.28 ± 2.43 to 72.19 ± 1.40 μg mL-1). The obtained extracts also demonstrate anti-hemolytic activity (52.22 ± 1.62 to 71.17 ± 1.82%). Among the phenolic compounds identified, protocatechuic, vanillic and β-resorcylic acids were the most abundant (1.13 ± 0.06 to 2.53 ± 0.06, 0.90 ± 0.06 to 2.19 ± 0.06 and 1.03 ± 1.62 to 22.11 ± 1.62 mg L-1, respectively). In the statistical analysis, a significant correlation was found between the flavonoids content and all enzymes inhibitory activities. The present study showed that D. ecastaphyllum leaves extracts may have the potential to be used in the therapeutic treatment of several diseases such as Alzheimer, Parkinson, type 2 diabetes mellitus, hyperglycemia, and pigmentation, as well as those associated with oxidative stress.

scholarly journals Isolation and identification of 3-acetylecdysone 2-phosphate, a metabolite of ecdysone, from developing eggs of Schistocerca gregaria

1984 ◽  
Vol 217 (1) ◽  
pp. 239-243 ◽  
Author(s):  
R E Isaac ◽  
H P Desmond ◽  
H H Rees
Keyword(s):  
High Performance ◽  
Schistocerca Gregaria ◽  
Helix Pomatia ◽  
Reversed Phase ◽  
Ionization Mass ◽  
Isolation And Identification ◽  
Ecdysteroid Conjugate ◽  
Hydrolysis Of

A major ecdysteroid conjugate, which accumulates in the eggs of the desert locust, Schistocerca gregaria, during the later stages of embryogenesis, has been isolated by reversed-phase and anion-exchange high-performance liquid chromatography. Hydrolysis of the conjugate with a crude arylsulphatase preparation from Helix pomatia liberates mainly ecdysone 3-acetate. The compound was identified as 3-acetylecdysone 2-phosphate by phosphate analysis of an acid-hydrolysed sample, fast atom bombardment, electron impact and chemical ionization mass spectrometry and 1H and 13Cn.m.r. spectroscopy. The instability of 3-acetylecdysone 2-phosphate on storage results in the formation of ecdysone 2-phosphate, which was identified by physicochemical techniques. 3-Acetylecdysone 2-phosphate and ecdysone 2-phosphate are less susceptible than ecdysone 22-phosphate to hydrolysis in vitro by an enzyme preparation from S. gregaria embryos. The possible role of 3-acetylecdysone 2-phosphate as an inactive end product of ecdysteroid metabolism is discussed.

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