scholarly journals Fibroblasts as the subject of proliferative activity research in vitro

2020 ◽  
Vol 5 (3) ◽  
pp. 210-215
Author(s):  
Valeriya I. Kuzmicheva ◽  
Larisa T. Volova ◽  
Frida N. Gilmiyarova ◽  
Ilya M. Bykov ◽  
Elena V. Avdeeva ◽  
...  
Keyword(s):  
Free Radical ◽  
Proliferative Activity ◽  
Fibroblast Cell ◽  
Biologically Active ◽  
Proliferative Potential ◽  
Metabolic Processes ◽  
Fibroblast Cell Culture ◽  
The Stability ◽  
Radical Processes

This review presents the data devoted to anatomical and functional diversity of fibroblasts, peculiarities of metabolic processes and energy exchange in these cells. In particular, the changes in fibroblast proliferative activity depending on various factors are discussed. The review shows the influence of the malate dehydrogenase shuttle system on the activity of metabolic processes and the life span of fibroblastsin vitro. The increase of cell cultivation timein vitrois associated with the cytosolic isoform of this enzyme. The stability of fibroblast cell culture to the activation of free-radical processes and peroxidation with addition of biologically active compounds is described and followed by a discussion of the role of separate metabolites in providing free-radical protection and maintenance of the proliferative potential of cells.

scholarly journals Ozonized Water in Microbial Control: Analysis of the Stability, In Vitro Biocidal Potential, and Cytotoxicity

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 525
Author(s):  
Laerte Marlon Conceição dos Santos ◽  
Eduardo Santos da Silva ◽  
Fabricia Oliveira Oliveira ◽  
Leticia de Alencar Pereira Rodrigues ◽  
Paulo Roberto Freitas Neves ◽  
...  
Keyword(s):  
Microbial Control ◽  
Fibroblast Cell ◽  
In Vitro Assays ◽  
Control Analysis ◽  
Cytotoxic Effects ◽  
Cell Wall Lysis ◽  
Osmotic Stability ◽  
Human Ear ◽  
The Stability

O3 dissolved in water (or ozonized water) has been considered a potent antimicrobial agent, and this study aimed to test this through microbiological and in vitro assays. The stability of O3 was accessed following modifications of the physicochemical parameters of water, such as the temperature and pH, with or without buffering. Three concentrations of O3 (0.4, 0.6, and 0.8 ppm) dissolved in water were tested against different microorganisms, and an analysis of the cytotoxic effects was also conducted using the human ear fibroblast cell line (Hfib). Under the physicochemical conditions of 4 °C and pH 5, O3 remained the most stable and concentrated compared to pH 7 and water at 25 °C. Exposure to ozonized water resulted in high mortality rates for Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, and Candida albicans. Scanning electron micrograph images indicate that the effects on osmotic stability due to cell wall lysis might be one of the killing mechanisms of ozonized water. The biocidal agent was biocompatible and presented no cytotoxic effect against Hfib cells. Therefore, due to its cytocompatibility and biocidal action, ozonized water can be considered a viable alternative for microbial control, being possible, for example, its use in disinfection processes.

scholarly journals Regulation of erythropoietin production in a human hepatoblastoma cell line

Blood ◽  
1987 ◽  
Vol 70 (6) ◽  
pp. 1904-1909
Author(s):  
OJ Nielsen ◽  
SJ Schuster ◽  
R Kaufman ◽  
AJ Erslev ◽  
J Caro
Keyword(s):  
Cell Line ◽  
Northern Blot ◽  
Proliferative Activity ◽  
Northern Blot Analysis ◽  
Cobalt Chloride ◽  
Biologically Active ◽  
Erythropoietin Production ◽  
Rna Probe ◽  
Hepatoblastoma Cell Line

Production of immuno and biologically active erythropoietin was documented to occur in the human hepatoblastoma cell line HepG-2. The expression of the erythropoietin gene was further verified by Northern blot analysis using a single stranded RNA probe. In vitro studies showed that erythropoietin production by these cells was not stimulated by hypoxia or cobalt chloride, but was related to the proliferative activity of the cells in culture. In addition it was found that the secretion of erythropoietin was almost completely abrogated by tunicamycin, an inhibitor of N-linked glycosylation. This effect of tunicamycin was also observed in a permanently transfected cell line that secretes erythropoietin in large quantities.

scholarly journals Development of Bionanocomposites Based on PLA, Collagen and AgNPs and Characterization of Their Stability and In Vitro Biocompatibility

2020 ◽  
Vol 10 (7) ◽  
pp. 2265
Author(s):  
Maria Râpă ◽  
Laura Mihaela Stefan ◽  
Traian Zaharescu ◽  
Ana-Maria Seciu ◽  
Anca Andreea Țurcanu ◽  
...  
Keyword(s):  
Normal Growth ◽  
Fibroblast Cell ◽  
Stability Study ◽  
Mouse Fibroblast ◽  
Poly Lactic Acid ◽  
In Vitro Biocompatibility ◽  
Diphenyltetrazolium Bromide ◽  
Mouse Fibroblast Cell Line ◽  
The Stability

Bionanocomposites including poly(lactic acid) (PLA), collagen, and silver nanoparticles (AgNPs) were prepared as biocompatible and stable films. Thermal properties of the PLA-based bionanocomposites indicated an increase in the crystallinity of PLA plasticized due to a small quantity of AgNPs. The results on the stability study indicate the promising contribution of the AgNPs on the durability of PLA-based bionanocomposites. In vitro biocompatibility conducted on the mouse fibroblast cell line NCTC, clone 929, using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed high values of cell viability (>80%) after cell cultivation in the presence of bionanocomposite formulations for 48 h, while the percentages of lactate dehydrogenase (LDH) released in the culture medium were reduced (<15%), indicating no damages of the cell membranes. In addition, cell cycle analysis assessed by flow cytometry indicated that all tested bionanocomposites did not affect cell proliferation and maintained the normal growth rate of cells. The obtained results recommend the potential use of PLA-based bionanocomposites for biomedical coatings.

scholarly journals The effect of 5-fluorouracil on erythropoiesis

Blood ◽  
1991 ◽  
Vol 77 (6) ◽  
pp. 1164-1170 ◽  
Author(s):  
IN Rich
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Normal Values ◽  
Biologically Active ◽  
Stem Cell Population ◽  
Enzyme Linked Immunosorbent Assay ◽  
Proliferative Potential ◽  
Colony Forming Unit ◽  
Kinetics Of

Abstract The effects of a single dose (150 mg/kg) of 5-fluorouracil on mature erythroid and erythropoietic and multipotential in vitro precursor populations in the bone marrow and spleen and circulating biologically (erythroid colony forming unit [CFU-E] assay) and immunologically active (enzyme-linked immunosorbent assay) erythropoietin (Epo) are described. All mature erythroid (reticulocytes, erythrocytes) and in vitro erythropoietic precursors (CFU-E, erythroid burst-forming unit [BFU-E]) are severely reduced, if not eradicated. Transient repopulation of the pure BFU-E and CFU-E populations on days 6 and 7, respectively, produces a marked reticulocytosis after day 9. Circulating Epo increases to above normal values by day 2. However, whereas biologically active Epo remains constant at this level until day 9, immunologically active Epo continually increases; by day 12, however, both assays detect circulating Epo levels of about 400 mU/mL. In vitro multipotential stem cells (BFU-E mix) are reduced to 32% on day 1, 7.6% on day 2, and return to normal values between days 4 and 5. The survival and repopulation kinetics of the BFU-E mix imply a stem cell population more mature than the high proliferative potential colony-forming cells. However, the BFU-E mix may be responsible for erythropoiesis repopulating ability.

The Behavior of SiC Films Fabricated by Hybrid Laser-Magnetron Deposition after Immersion

2007 ◽  
Vol 330-332 ◽  
pp. 537-540 ◽  
Author(s):  
Nicoleta Maria Badea ◽  
M. Jelinek ◽  
T. Tite ◽  
Mariana Prodana ◽  
A. Campean ◽  
...  
Keyword(s):  
Adsorption Process ◽  
Biological Response ◽  
Fibroblast Cell ◽  
Human Blood Plasma ◽  
Ftir Analysis ◽  
In Vitro Bioactivity ◽  
Magnetron Deposition ◽  
Fibroblast Cell Culture ◽  
Sic Films

The in-vitro bioactivity of SiC growing using the innovative hybrid laser-magnetron deposition technique was investigated. The biological response has been analysed after being immersed in a human blood plasma solution for 10 days. Surface analysis by SEM microscopy shows clearly an adsorption process after immersion. The products formed are mainly attributed to a rich-calcium phosphate and silica-complexes layers. Citotoxicity test shows a normal phenotype of fibroblast cell culture on SiC and MTT assay indicates an increased viability of cells around 15% after only one day of immersion. Results obtained are supported by FTIR analysis. Strong changes in the absorbance of bands after immersion are observed, which indicates a strong bioactivity of SiC.

scholarly journals The effect of 5-fluorouracil on erythropoiesis

Blood ◽  
1991 ◽  
Vol 77 (6) ◽  
pp. 1164-1170
Author(s):  
IN Rich
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Normal Values ◽  
Biologically Active ◽  
Stem Cell Population ◽  
Enzyme Linked Immunosorbent Assay ◽  
Proliferative Potential ◽  
Colony Forming Unit ◽  
Kinetics Of

The effects of a single dose (150 mg/kg) of 5-fluorouracil on mature erythroid and erythropoietic and multipotential in vitro precursor populations in the bone marrow and spleen and circulating biologically (erythroid colony forming unit [CFU-E] assay) and immunologically active (enzyme-linked immunosorbent assay) erythropoietin (Epo) are described. All mature erythroid (reticulocytes, erythrocytes) and in vitro erythropoietic precursors (CFU-E, erythroid burst-forming unit [BFU-E]) are severely reduced, if not eradicated. Transient repopulation of the pure BFU-E and CFU-E populations on days 6 and 7, respectively, produces a marked reticulocytosis after day 9. Circulating Epo increases to above normal values by day 2. However, whereas biologically active Epo remains constant at this level until day 9, immunologically active Epo continually increases; by day 12, however, both assays detect circulating Epo levels of about 400 mU/mL. In vitro multipotential stem cells (BFU-E mix) are reduced to 32% on day 1, 7.6% on day 2, and return to normal values between days 4 and 5. The survival and repopulation kinetics of the BFU-E mix imply a stem cell population more mature than the high proliferative potential colony-forming cells. However, the BFU-E mix may be responsible for erythropoiesis repopulating ability.

scholarly journals In vitro Study Anti-proliferative Potential of Algae Extract against Cancer Cell Line

2019 ◽  
pp. 1-10
Author(s):  
Morog R. Maddah ◽  
Etimad A. Huwait ◽  
Abeer Ali Al-balawi ◽  
Said S. Moselhy ◽  
Maryam A. ALghamdi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Marine Organism ◽  
Biological Activities ◽  
Cancer Cell Line ◽  
In Vitro Study ◽  
Positive Control ◽  
Biologically Active ◽  
Sulfated Polysaccharides ◽  
Proliferative Potential

Background: Oxidative stress is defined as imbalance between oxidant and antioxidant ratio, that lead to oxidative damage of biologically active molecules, finally lead to different disease and initiate carcinogenesis. The drug discovery using natural products as medicinal plants or marine organism still an important target for recent research. This study investigated anticancer activity of algae extract obtained from Red sea at Jeddah. Materials and Methods: Aqueousand methanol extracts ofDictyotaciliolata(DC) were tested on HCT-116 and HepG2 cell lines using WST-1. Aqueous extract (AEDC) and methanol extract (MEDC) at doses 0.05, 0.1, 0.5, 1 mg/ml and positive control 0.3% H2O2 at doses 0.5 mg/ml for 24,48 and 72 h (for two cell lines). Results: AEDC showed the mosteffective antitumor activity against HCT116 and HePG2, the IC50 dose for HCT116 cells was 0.05 mg/ml at 72 h, while for the HePG2 it was 0.01 mg/ml at 72 h. These results showed that HePG2cells was more sensitive to the AEDC. However, IC50 for MEDC were 0.01 mg/ml for HCT116 and 0.05 for HepG2 at 48 Hrs. The algae extracts contain sulfated polysaccharides and different pigments as chlorophylls, carotenoids and phycobiliproteins. These pigments were approved as biological active biomolecules that exert different biological activities as antioxidants, antitumor and rich source of micronutrients. In addition, AEDC or MEDC exert apoptotic activity by increase activity of caspase 3 and 9 in HepG2. Conclusıon: The antitumor effect of AEDC or MEDC is promising for development of chemotherapeutic agent as effective with no side effects.

Identification of metabolites of peptide-derived drugs using an isotope-labeled reporter ion screening strategy

2020 ◽  
Vol 58 (5) ◽  
pp. 690-700 ◽  
Author(s):  
Andreas Thomas ◽  
Mario Thevis
Keyword(s):  
Stable Isotope ◽  
High Resolution Mass Spectrometry ◽  
Degradation Products ◽  
Screening Strategy ◽  
Biologically Active ◽  
Recombinant Human Insulin ◽  
Metabolic Processes ◽  
Metabolic Products

AbstractBackgroundPeptide-derived drugs represent an emerging class of prohibited substances in professional sports and, thus, in modern doping controls. After parental administration (e.g. subcutaneous, intravenous), these drugs undergo various metabolic processes, which degrade them to biologically active or inactive peptides. Knowledge about these metabolic processes and the hereby produced metabolites plays a key role in successful doping controls due to the effective design of analytical assays under consideration of optimal analytical targets. Unfortunately, the complexity of biological matrix (e.g. blood or urine) complicates the immediate identification of relevant metabolites due to the enormous excess of naturally occurring peptides and their degradation products.MethodsIn this study, a strategy employing in-vitro metabolism of stable isotope-labeled peptides producing characteristic reporter ions derived from labeled immonium ions is shown. The in-vitro experiments were performed with human skin tissue microsomes (S9), and model drugs representing prohibited peptide hormones were synacthen, insulin, and corticorelin (respectively, their stable isotope-labeled analogs). After generic sample preparation, the metabolites were identified by means of liquid chromatography (LC) coupled to high-resolution mass spectrometry (MS) in an untargeted approach.Results and conclusionsFor all three model peptides, several metabolic products were readily identified. While insulin and corticorelin were found to be comparably stable, synacthen was fully degraded, yielding a plethora of metabolic products. A proof of concept concerning the transferability of the obtained data was accomplished by analyzing plasma samples collected post-administration of recombinant human insulin, corroborating the presence of a skin protease-indicative insulin metabolite in vivo.

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