scholarly journals UJI DAYA HAMBAT EKSTRAK ETANOL DAUN KACAPIRING (Gardenia augusta) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli DAN Salmonella thypi

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 268
Author(s):  
Serly D. S. Toding ◽  
Herny E. I. Simbala ◽  
Deby A. Mpila
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Inhibitory Power ◽  
Paper Disc

ABSTRACT One of the potential medicinal plants as traditional medicine is gardenia plant (Gardenia augusta). Gardenia leaf extract contains alkaloid compounds, flavonoids, tannins, saponins and triterpenoids, which are known to have antibacterial abilities..The aim of this research was to determine the antibacterial activity of gardenia leaf extract on the growth of Staphylococcus aureus, Escherichia coli and Salmonella thypi. The examination on the inhibitory power using the Kirby-Bauer method. The Kirby-Bauer method is referring to the diffusion method using paper disc with five treatments, namely the extract of gardenia leaves with a substance concentration of 20%, 40%, and 60% as well positive control (Ciprofloxacin) and negative control (aquades). Based on the research results obtained, it can be concluded that gardenia leaf extract can inhibit growth of Staphylococcus aureus, Escherichia coli and Salmonella thypi at a concentration of 20%, 40% and 60% and classified as strong and medium category. Keywords  :    Gardenia augusta, antibacterial, of Staphylococcus aureus, Escherichia coli, Salmonella thypiABSTRAK  Salah satu tanaman obat dengan potensi sebagai obat tradisional adalah tanaman kacapiring (Garddenia augusta). Daun kacapiring mengandung senyawa alkaloid, flavonoid, tanin, saponin dan triterpenoid, yang berpotensi sebagai antibakteri sehingga digunakan dalam penelitian ini.Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak daun kacapiring terhadap pertumbuhan Staphylococcus aureus, Escherichia coli dan Salmonella thypi. Pengujian daya hambat menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas dengan 5 perlakuan yaitu ekstrak daun kacapiring dengan konsentrasi 20%, 40%, dan 60% serta kontrol positif (ciprofloxacin) dan kontrol negatif (akuades). Berdasarkan hasil penelitian yang diperoleh, dapat disimpulkan bahwa ekstrak daun kacapiring dapat menghambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Salmonella thypi pada konsentrasi 20%, 40% dan 60% dan tergolong dalam kategori kuat dan sedang. Kata Kunci  : Kacapiring, Antibakteri, Staphylococcus aureus, Escherichia coli, Salmonella thypi

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI KARANG LUNAK Sinularia SP. DI TELUK MANADO

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 114
Author(s):  
Lyone Katiandagho ◽  
Defny S Wewengkang ◽  
Sri Sudewi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Soft Coral ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Chloroform Fraction ◽  
Water Fraction

ABSTRACT Soft Coral Sinularia sp.has been shown to have antibacterial activity. This research aims to determine the antibacterial activity of marine  organisms obtained from Manado bay. The method in this study is the diffusion method with positive control chloramphenicol and negative control of methanol in Escerichia coli and Sthaphylococcus aureus. The result showed that extracts, chloroform fraction and methanol-water fraction were obtained inhibiting category of Sthapylococcus aureus bacteria, but in the chloroform fraction and ethanol extract can inhibit Escerichia coli bacteria categorized as strong. With it can be conlueded that the extract and fraction of soft Sinularia sp. have bioactive compounds with a broth spectrum of antibacterial activity.Keywords: Sinularia sp., Chloramphenicol, Escerichia coli, Sthaphylococcus aureus, and antibacterial. ABSTRAK Karang lunak Sinularia sp. telah terbukti memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri pada organisme biota laut yang diperoleh dari teluk Manado. Metode dalam penelitian ini adalah metode difusi agar dengan kontrol positif kloramfenikol dan kontrol negatit metanol pada Escherichia coli dan Staphylococcus aureus. Hasil penelitian menunjukkan bahwa ekstrak, fraksi kloroform, dan fraksi metanol-air dapat menghambat bakteri Staphylococcus aereus dikategorikan sedang, namun pada fraksi kloroform dan ekstrak etanol dapat menghambat bakteri Escerichia coli dikategorikan kuat. Dengan demikian dapat disimpulkan bahwa ekstrak dan fraksi karang lunak Sinularia sp.memiliki senyawa bioaktif dengan spektrum yang luas terhadap aktivitas antibakteri.Kata kunci: Sinularia sp, Kloramfenikol, Escherichia coli, Staphylococcus aureus, dan antibakteri

scholarly journals The Antibacterial Activity of Thermoactinomyces sp. (H24) Extract Against Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 3 (1) ◽  
pp. 56-63
Author(s):  
Julia Nanda Puspita ◽  
Rikhsan Kurniatuhadi ◽  
Rahmawati Rahmawati
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Bioactive Compound ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
And Staphylococcus Aureus

Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extract from Thermoactinomyces sp. (H24) against Escherichia coli and Staphylococcus aureus. Methanol was used as a solvent for the extraction of bacterial bioactive compounds. Antibacterial activity was analyzed by the diffusion method with several extract concentrations (0.75 mL, 1.5 mL, 2.25 mL, and 3 mL), 10% DMSO as the negative control, and ciprofloxacin as the positive control. Our result shows that Termoactinomyces sp. (H24) extract has an inhibitory effect on the growth of  E. coli and S. aureus with an effective concentration of 2.25 mL (inhibition strength: very strong).

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK BIJI BUAH DUKU Lansium domesticum TERHADAP BAKTERI STAPHYLOCOCUS AUREUS DAN ESCHERICHIA COLI

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 818
Author(s):  
Albrita Pehino ◽  
Fatimawali Fatimawali ◽  
Elly J. Suoth
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Nutritional Value ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Activity Test ◽  
Solution Studies ◽  
Lansium Domesticum

ABSTRACT Duku (Lansium domesticum) provides many benefits for the community. Apart from the fruit which has high nutritional value, duku is believed by the community to have benefits. The study aims to learn about the antibacterial extract of my seed against the staphylococus aureus and escherichia coli. This study used the meseration method with ethanol as a solvent. The antibacterial activity test used the well diffusion method with a concentration of 10%, 20%, 30%, 40%. The positive control used was Ciprofloxacin and the negative control used was the CMC solution. Studies show that the suppression of extract is 10%, 20%, 30%, and 40% staphylococus aureus, the average drip zone is 11.3mm, 13.3mm, 13.6mm, 11,3mm, and escherichia coli, on average, 10 mm,  9,3mm, 10,3mm, 12,6 mm. The results suggest that the extract of the duku fruit has antibacterial activity against the growth of the staphylococcus aureus and escherichia coli. Keywords: Duku seed, Antibacterial, Antibacterial activity ABSTRAK Duku (Lansium domesticum) banyak memberikan manfaat bagi masyarakat. Selain buahnya yang mempunyai nilai gizi tinggi, duku dipercaya masyarakat memiliki manfaat untuk kesehatan. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak biji duku terhadap bakteri staphylococus aureus  dan Escherichia coli. Penelitian ini menggunakan metode maserasi dengan pelarut etanol. Uji aktivitas antibakteri menggunakan metode difusi sumuran  dengan konsentrasi 10%, 20%, 30%, 40%. Kontrol positif yang digunakan adalah  Ciprofloxacin dan kontrol negatif yang digunakan adalah larutan CMC.  Hasil penelitian menunjukan bahwa daya hambat ekstrak dengan konsentrasi 10%, 20%, 30%, dan 40% Staphylococcus aureus rata-rata  zona beningnya 11,3mm, 13,3mm, 13,6mm, 11,3mm, dan Escherichia coli rata-rata  zona beningnya 10 mm, 9,3mm, 10,3mm, 12,6 mm. Dari hasil yang didapatkan  disimpulkan bahwa ekstrak biji buah duku memiliki aktivitas antibakteri terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Kata Kunci:Biji buah duku, Antibakteri, Aktivitas antibakteri

UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

scholarly journals Pengujian Aktivitas Antibakteri Hidrogel Ekstrak Etanol Daun Tagetes erecta L

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 96
Author(s):  
Hosea Jaya Edy ◽  
Marchaban Marchaban ◽  
Subagus Wahyuono ◽  
Agung Endro Nugroho
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Tagetes Erecta ◽  
Tagetes Erecta L

Kandungan kimia flavonoid dan fenolik dalam ekstrak daun T.erecta diketahui memiliki aktivitas antibakteri yang baik. Pengunaan ekstrak daun T.erecta sebagai zat aktif sediaan hidrogel akan sangat mempermudah pemanfaatan dalam proses terapi oleh masyarakat. Tujuan dalam penelitian ini adalah melihat kemampuan aktivitas antibakteri sediaan hidrogel dengan zat aktif ekstrak daun T.erecta. Dalam penelitian ini digunakan 6 variasi konsentrasi ekstrak yang terkandung pada sediaan hidrogel yaitu 1.5; 2.0; 2.5; 3.0; 3.5; dan 4.0 %. Pengujian aktivitas antibakteri menggunakan metode difusi sumuran terhadap bakteri Staphylococcus aureus dan Escherichia coli. Aktivitas antibakteri terbesar ditunjukkan oleh sediaan hidrogel dengan konsentrasi ekstrak daun T.erecta 2.5%. Diameter zona hambat yang terbentuk terhadap pertumbuhan E.coli adalah 15.33 mm sedangkan diameter zona hambat terhadap S.aureus adalah 17.87 mm. Untuk kontrol positif digunakan bioplacenton® dan untuk kontrol negatif digunakan basis hidrogel tanpa kandungan ekstrak T.erectaFlavonoid and phenolic on ethanolic extract of T.erecta L has been antibacterial activity. The aim of this research was to determinate of antibacterial from hydrogel with ethanolic extract of T.erecta L. This research was begun making hydrogels with six variations of ethanolic extract of T.erecta L were 1.5; ; 2.0; 2.5; 3.0; 3.5; and 4.0 %.  Antibacterial activity used hole diffusion method against Staphylococcus aureus and Escherichia coli. Hydrogel containing 2.5 % ethanolic extract of T.erecta L was shown the highest antibacterial activity. The diameters of the inhibition zone against E.coli was 15.33 mm and against S.aureus was 17.87 mm. Positive control used bioplacenton® and negative control was hydrogel without ethanolic extract of T.erecta content

scholarly journals Antibacterial Activity Test of the C-4-methoxyphenylcalix[4]resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria

2018 ◽  
Vol 3 (3) ◽  
pp. 201 ◽  
Author(s):  
Suryadi Budi Utomo ◽  
Mita Fujiyanti ◽  
Warih Puji Lestari ◽  
Sri Mulyani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Hexadecyltrimethylammonium Bromide ◽  
E Coli ◽  
Escherichia Coli Bacteria

<p>This study aims to determine the antibacterial activity of C-4-methoxyphenylcalix-[4]resorcinarene modified by Hexadecyltrimethylammonium-Bromide (Resorcinarene-HDTMA-Br) against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> bacteria. The study was conducted by testing the inhibition zone diameter with paper disc diffusion method. Variations in the concentration of the sample used were 10%; 15%; 20%; 25%; and 30% b/v. In this study, Dimethyl sulfoxide (DMSO) (sampel solvent) was used as a negative control and Chloramphenicol as a positive control. It was found that Resorsinarene-HDTMA-Br had higher antibacterial activity than the unmodified C-4-methoxyphenylcalix[4]resorcinarene (CMFKR) against the <em>S.</em><em> </em><em>aureus</em> bacteria. Both CMFKR and Resorcinarene-HDTMA-Br are not active against <em>E. coli</em>.</p>

scholarly journals ANTIBACTERIAL ACTIVITY OF KARAMUNTING (RHODOMYRTUS TOMENTOSA (AITON) HASSK) LEAF EXTRACT AND FRACTIONS

2018 ◽  
Vol 11 (3) ◽  
pp. 163
Author(s):  
Siska Esperanza Sinulingga ◽  
Poppy Anjelisa Zaitun Hasibuan ◽  
Dwi Suryanto
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Rhodomyrtus Tomentosa ◽  
Agar Well Diffusion Method

 Objective: This study was to analyze the karamunting leaf extract and fractions effectiveness against Staphylococcus aureus and Pseudomonas aeruginosa infection.Methods: Phytochemical screening is done in the extract and fractions of karamunting leaf continue with Standard agar well diffusion method for S. aureus and P. aeruginosa. Dimethyl sulfoxide was used as negative control, and standard antibiotic Kalmicetine (chloramphenicol) was used as positive control. Result: The ethyl acetate fraction showed the highest antibacterial activity against S. aureus and P. aeruginosa. Conclusion: Karamunting leaf can be used to treat the bacterial infection diseases especially infection from S. aureus and P. aeruginosa.

scholarly journals Phytocimia Test And Antibacterial Bioactivity Of Extracts from Stem Bark of Artocarpus integer (Thunb.) Merr

2019 ◽  
Vol 12 (2) ◽  
pp. 84
Author(s):  
Liska Hamzah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Stem Bark ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Chloroform Fraction ◽  
Agar Media ◽  
And Staphylococcus Aureus

This research aims to identify the compound group and determine the antibacterial activity of n-hexane, chloroform, and methanol fractions of Artocarpus integer (Thunb.) Merr stem bark. All fractions contain groups of terpenoid and steroid compounds. Antibacterial activity was detemined using agar diffusion method with MHA (Muller Hinton Agar) media on Escherichia coli (gram negative) and Staphylococcus aureus (gram positive). Ciprofloxacin was used as a positive control and methanol as a negative control. The three fractions were able to inhibit the growth of Escherichia coli and Staphylococcus aureus. The chloroform fraction showed the highest activity after 48 hours incubation with inhibition zones of 10.8 and 10.4 mm respectively.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 19-23
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK PERASAN DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP Escherichia coli SECARA IN VITRO

2020 ◽  
Vol 2 (1) ◽  
pp. 26-34
Author(s):  
Margareta Retno Priamsari ◽  
Agastia Cicilia Wibowo
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Drying Shrinkage ◽  
Positive Control ◽  
Negative Control ◽  
Morinda Citrifolia ◽  
Diffusion Method ◽  
Juice Extraction ◽  
Water Inhibition

Noni juice can inhibit the growth of Escherichia coli bacteria. Noni juice extraction needs concentration to extract so that the preparation is more stable in the storage process. The purpose of this study was to determine the antibacterial activity and the amount of the minimum inhibitory concentration of noni juice extract from E. coli bacteria in vitro. This type of experimental research with a completely randomized one-way design. The extract was obtained by concentrating the Noni leaf extract. Extract quality control parameters include organoleptic, yield, drying shrinkage, and qualitative tests of flavonoid and anthraquinone compounds. Antibacterial activity test using the disc diffusion method with an extract concentration of 1.56%; 3.12%; 6.25%; 12.5%; and 25% with 3 replications. Positive control of amoxicillin and negative control of distilled water. Inhibition is known from the zone formed around the paper disc. The data obtained were statistically analyzed using Kruskall Wallis followed by Mann Whitney with a 95% confidence level. The results showed that the variation in the concentration of the noni juice extract had a significant effect (p <0.05). The biggest inhibitory zone was seen at 25% concentration of 10.16 mm and included in the strong category. The minimum inhibitory power was produced at a concentration of 3.12% at 2.50 mm with a weak treatment category.

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