ANTIMICROBIAL EFFICACY OF CARABAO GRASS LEAVES ON STAPHYLOCOCCUS AUREUS

Antimicrobial Efficacy of Carabao Grass (Paspalum conjugatum) leaves on Staphylococcus aureus Dani Aiko Garduque1, Kyrie Radiance Mateo1, Shalom Moyinoluwa Oyinloye1, and Jo Anne Kristine Lalas-Lucero2 1Student, Adventist University of the Philippines 2Faculty, Adventist University of the Philippines [email protected] Abstract Introduction: In the Philippines, the Paspalum conjugatum or known by its common name as “carabao grass” is usually consumed for livestock as well as for landscaping. Paspalum conjugatum has been known to have phytochemicals that kill microorganisms. This experimental research determined the antimicrobial efficacy of Paspalum conjugatum against Staphylococcus aureus. Methods: Samples of the carabao grass was purchased, washed and then dried. An extract was obtained from the sample. The extract was then sent to an accredited testing center for phytochemical test to identify the different components of the plant extract. The antimicrobial activity of the paspalum conjugatum extract was then determined through the use of the disc diffusion method. The zone of inhibition of Paspalum Conjugatum was also compared to a control standard such as oxacillin. Results: The results of the study revealed to have abundant constituents of sterols, flavonoids and tannins; moderate levels of triterpenes, saponins and glycosides; and traces of alkaloids. The antimicrobial testing showed results that Paspalum Conjugatum had 10mm zone of inhibition which means that it has a complete inhibitory activity with severe reactivity against the tested organism, staphylococcus aureus. Discussion: The study concludes that Paspalum conjugatum can be used to eliminate staphylococcus aureus. It is recommended that further studies be conducted for the use of Paspalum conjugatum in the treatment and prevention of diseases caused by staphylococcus aureus. Keywords: paspalum conjugatum, carabao grass, staphylococcus aureus

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Screening of the antimicrobial activity of marine sponge extracts from Martinique

10.1101/2021.02.16.431398 ◽

2021 ◽

Author(s):

Julie Piron ◽

Jessica Pastour ◽

Niklas Tysklind ◽

Juliette Smith-Ravin ◽

Fabienne Priam

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Marine Sponges ◽

Diffusion Method ◽

Disc Diffusion ◽

Bacterial Strains ◽

Disc Diffusion Method ◽

Staphylococcus Saprophyticus

AbstractMarine sponges are known for their antimicrobial, antifungal, and cytotoxic activity. In this study, the activity of aqueous and ethanoic extracts of 3 sponges from Martinique were tested on 5 bacterial strains: Bascillus cereus (CIP 783), Echerichia coli (CIP 54127), Pseudomonas aeruginosa (CIP A22), Staphylococcus aureus (CIP 67.8) and Staphylococcus saprophyticus (CIP 76125). The antimicrobial activity of Agelas clathrodes, Desmapsamma anchorata, and Verongula rigida, was demonstrated using the disc diffusion method and by determining the minimum inhibitory concentration and the minimum bactericidal concentration. The ethanoic extract of Agelas clathrodes had an inhibitory activity specifically on Staphylococcus aureus and Staphylococcus saprophyticus. No activity was observed for the other extracts. Further chemical analyses will be carried out in order to identify the active molecules of these sponges.

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Antimicrobial Activity of some Honey Samples against Pathogenic Bacteria

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.34.15 ◽

2015 ◽

Vol 34 ◽

pp. 15-20 ◽

Cited By ~ 4

Author(s):

S. Mahendran ◽

D. Kumarasamy

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Antibacterial Activity ◽

Streptococcus Pyogenes ◽

Western Ghats ◽

Pathogenic Bacteria ◽

Research Work ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method

The aim of the present research work to investigate antimicrobial activity of some honey samples six winter honeys six summer honeys collected from different regions of Western Ghats. The microbes used in this study are Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis. Antibacterial activity of the honeys was assayed using the Disc diffusion method. Noticeable variations in the antibacterial activity of the different honey samples were observed. Among the microbes Staphylococcus aureus is the most sensitive against all honey samples shows the maximum inhibitor zone compare to summer honeys.

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Antimicrobial Properties of 6-Bromoeugenol and Eugenol

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.53.57 ◽

2016 ◽

Vol 53 ◽

pp. 57-64

Author(s):

Radia Mahboub ◽

Faiza Memmou

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Inhibitory Concentration ◽

Antimicrobial Properties ◽

Diffusion Method ◽

Disc Diffusion ◽

Bacterial Strains ◽

Disc Diffusion Method ◽

Modest Activity

We have studied the antimicrobial properties of 6-bromoeugenol and eugenol by three strains:Pseudomonas aeruginosa(S1),Escherichia coli(S2) andStaphylococcus aureus(S3). We have determined the minimum inhibitory concentration (MIC) for a range of concentrations using the disc diffusion method. We note that all samples present an antimicrobial activity toward the tested bacterial strains at different concentrations (1, 0.5 and 0.25 mg/ml). The 6-bromoeugenol gives modest activity with (S1) and (S3). Eugenol reacts positively with thePseudomonas aeruginosa(S1) at all concentrations and with theEscherichiacoli(S2) at 0.5 mg/ml. We remark that thePseudomonas aeruginosa(S1) is the more sensitive strain thanEscherichiacoli(S2) andStaphylococcus aureus(S3). We have estimated the activity coefficient which has confirmed the antimicrobial activity of the different samples. So, 6-bromoeugenol has shown his efficiency as antimicrobial agent.

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Antioxidant and antimicrobial effect of alkaloid bulbs extract of Polianthes tuberosa L. (Amaryllidaceae) cultivated in Algeria

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i4.4134 ◽

2020 ◽

Vol 10 (4) ◽

pp. 44-48

Author(s):

Nihed BARGHOUT ◽

Nada CHEBATA ◽

Saida MOUMENE ◽

Seddik KHENNOUF ◽

Abdelaziz GHARBI ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Antimicrobial Effect ◽

Antimicrobial Activities ◽

Inhibitory Effect ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Scavenging Effect ◽

Alkaloid Extracts

Polianthes tuberosa L. (Amaryllidaceae) is an ornamental and medicinal plant. Its flowers and bulbs are used traditionally as a diuretic, emetic, against rashes and gonorrhea. The aim of this work was, to evaluate the antioxidant and antimicrobial activities of bulbs and bulbils alkaloid extracts of P. tuberosa. Antiradical effect was assessed against DPPH radical. However, antimicrobial activity was measured through the disc diffusion method against Escherichae coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Staphylococcus aureus resistant to Methicillin (MRSA) and Candida albicans ATCC 90028. The scavenging effect against DPPH showed that the bulbs and bulbils alkaloids extracts exhibited an antiradical effect with IC50 = 0.231±0.017 mg/mL and 0.233±0.093 mg/mL respectively, less than the effect of vitamin C with IC50 = 0.0194±0.0002 mg/mL. Antimicrobial activity results reveal that both alkaloid bulbs extracts at 50 mg/mL did not have any inhibitory effect against the studied strains using the disc diffusion method. According to this work, bulbs and bulbils alkaloid extracts show a moderate antioxidant effect; that could be recommended as a natural antioxidant. Although tuberose bulbs were used traditionally as a soap substitute; bulbs alkaloid extract has no antimicrobial effect. Keywords: Polianthes tuberosa L., bulbs, bulbils, alkaloids, antiradical activity, antimicrobial activity.

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Antimicrobial Activity of Cymbopogon (Lemongrass) on Enterococci

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i47b33170 ◽

2021 ◽

pp. 684-690

Author(s):

A. Sai Sanjith ◽

N. P. Muralidharan

Keyword(s):

Antimicrobial Activity ◽

Diffusion Method ◽

Dilution Method ◽

Disc Diffusion ◽

Zone Of Inhibition ◽

Disc Diffusion Method ◽

Antibiotic Susceptibility Test ◽

Lemongrass Oil ◽

Test Organisms ◽

Broth Dilution

Aim: The aim of this study was to investigate antimicrobial activity against Enterococcus bacteria. Materials and Methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by the Broth Dilution Method. The antibiotic susceptibility test against the test organisms was performed by the Disc Diffusion Method. Results and Discussion: Antimicrobial activity of Cymbopogon oil was examined against common Enterococcus bacteria using broth microdilution assay and the disc diffusion method. Zone of inhibition was seen when Cymbopogon oil was kept with enterococcus bacteria, this proves to be bacteriostatic. Conclusion: Lemongrass oil proved to be bacteriostatic against Enterococcus, further research to be done to implement Lemongrass oil as an antibacterial agent.

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Gayatri Mantra Chanting Helps Generate Higher Antimicrobial Activity of Yagya’s Smoke

Interdisciplinary Journal of Yagya Research ◽

10.36018/ijyr.v1i1.6 ◽

2018 ◽

Vol 1 (1) ◽

pp. 09-14 ◽

Cited By ~ 4

Author(s):

Ruchi Singh ◽

Sunil Kumar Singh

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Inhalation Therapy ◽

Diffusion Method ◽

Disc Diffusion ◽

Human Pathogens ◽

Zone Of Inhibition ◽

Disc Diffusion Method ◽

Plant Materials

Yagya (known as a fire-ritual) is an ancient Vedic Indian method of herbal inhalation therapy. It is also described as an excellent mode of environmental purification. Here, a comparative study of antimicrobial activity of smoke extract of Yagya and non-Yagya has been performed. ‘Yagya’ in this text, refers to the burning of wood, specific types of dried medicinal plant materials (hawan samagri) along with ghee with the citation of mantras, whereas the term Non-Yagya implies burning of wood, hawan samagri, along with ghee. In non-Yagya the mantra citation has not been done. An apparatus was designed to simulate the burning process, and the smoke fraction was captured for antimicrobial activity on human pathogens i.e Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, and Salmonella typh. Antimicrobial activity was performed using disc diffusion method. Antimicrobial data revealed that the ‘smoke-extract’ obtained from Yagya experiments with mantra chanting had higher zone of inhibition values compared to that of same experiment conditions except presence of mantra chanting suggesting an important role of the mantra chanting in Yagya for medicinal applications

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Perbandingan Aktivitas Antibakteri Ekstrak Bunga dan Daun Beluntas (Pluchea indica (L.) less.) Terhadap Bakteri Staphylococcus aureus

Prosiding Seminar Nasional Kesehatan ◽

10.48144/prosiding.v1i.711 ◽

2021 ◽

Vol 1 ◽

pp. 547-553

Author(s):

Hida Ilyana ◽

Dwi Bagus Pambudi

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Extraction Method ◽

Research Method ◽

Ethanol Extract ◽

Diffusion Method ◽

Disc Diffusion ◽

Zone Of Inhibition ◽

Clear Zone ◽

Disc Diffusion Method

AbstractChemical constituents contained in the beluntas plant, including alkaloids, essential oils, flavonoids, phenols and tannins have the ability to inhibit the growth of Staphylococcus aureus bacteria. This research uses maceration extraction method. Thick extracts of flowers and leaves were used with a series of extract concentrations. The purpose of this study was to determine differences in the effectiveness of the ethanol extract of the leaves and flowers of beluntas against the growth of Staphylococcus aureus bacteria. The research method used in this study is the disc diffusion method. Data was collected by measuring the diameter of the clear zone formed in each treatment. Extract concentrations used 5%, 7% and 9%. The extracts of flowers and leaves of beluntas (Pluchea indica (L.) Less) have antibacterial activity with a larger zone of inhibition in extracts of leaves of beluntas compared to extracts of leaves of beluntas.Keywords: beluntas : flowers; leaves; extract; antibacterial AbstrakKandungan kimia yang terdapat di dalam tanaman beluntas, diantaranya alkaloid, minyak atsiri, flavonoid, fenol dan tanin memiliki kemampuan untuk menghambat pertumbuhan bakteri Staphylococcus aureus.Penelitian ini menggunakan metode ekstraksi maserasi.Ekstrak kental bunga dan daundigunakan dengan seri konsentrasi ekstrak. Tujuan dari penelitian ini yaitu mengetahui perbedaan efektivitas aktivitas ekstrak etanol daun dan bunga beluntas terhadap pertumbuhan bakteri Staphylococcus aureus. Metode penelitian yang digunakan dalam penelitian ini yaitu metode difusi cakram disk. Pengumpulan data dilakukan dengan cara mengukur diameter zona bening yang terbentuk pada setiap perlakuan. Konsentrasi ekstrak digunakan 5%, 7% dan 9%.Ekstrak bunga dan daun beluntas (Pluchea indica (L.)Less) memiliki aktivitas sebagai anti bakteri dengan zona hambat yang lebih besar pada ekstrak daun beluntas dibandingkan dengan ekstrak daun beluntas.Kata kunci: Beluntas; bunga; daun; ekstrak; antibakteri

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ISOLASI DAN AKTIVITAS ANTI MIKROBA BAKTERI ASAM LAKTAT DARI FERMENTASI NIRA KELAPA SAWIT

BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) ◽

10.31289/biolink.v5i1.1707 ◽

2018 ◽

Vol 5 (1) ◽

pp. 51 ◽

Cited By ~ 1

Author(s):

Edy Fachrial ◽

Harmileni Harmileni

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Palm Oil ◽

Pathogenic Bacteria ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method

<p><em>The aim of the study was to isolate lactic acid bacteria (LAB) from palm oil sap and to investigate the anti microbial activity of LAB against pathogen bacteria, Staphylococcus aureus and Escherichia coli using disc diffusion method. Palm oil sap were enriched using spesific medium MRS (Man Rogosa Sharpe) broth overnight, serial dilution were conducted and the culture were spread on the surface of MRS agar and then incubated for 48 hours. The result showed the total colonies of LAB were 1,4 x 107 CFU/mL. 6 colonies were randomly selected namely EHN1, EHN2, EHN3, EHN4, EHN5 and EHN6. The selected colonies are classified as Gram positive and bacilli in morphology. Antimicrobial activity against pathogenic bacteria was investigated using disc diffusion method. Antimicrobial activity of isolates against E.coli and S.aureus were 12,2 mm; 9mm; 11mm; 11mm; 10,5mm; 12 mm dan 13,5mm; 11mm; 12mm; 11,5mm; 12mm; 12,5mm respectively. from these result it can be concluded that LAB isolated from oil palm sap has potential as probiotic.</em></p>

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EVALUATION OF ANTIBACTERIAL ACTIVITY OF A CYANOBACTERIUM SCYTONEMA SCHMIDTII GOM

10.36106/paripex/2805137 ◽

2021 ◽

pp. 77-79

Author(s):

Janardhan Namdeo Nehul

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Methanol Extract ◽

Axenic Culture ◽

Soil Samples ◽

Diffusion Method ◽

Disc Diffusion ◽

Zone Of Inhibition ◽

Disc Diffusion Method ◽

Air Blower

Scytonema schmidtii,a cyanobacterium was isolated from the collected soil samples from different locations of Ahmednagar district of Maharashtra state (India). Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959) and Prescott (1962) .The axenic culture of Scytonema schmidtii was obtained by using the method recommended by Bolch and Blackburn (1996). The isolated Scytonema schmidtii was grown autotropically in BG-11 medium as described by Rippka et al.,(1979) and incubated at 30±2°c.After 25 days, biomass was harvested by filtration through double layered muslin cloth and dried using air blower. The biomass of this Scytonema schmidtii species was used for the assessment of antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus flavus and Proteus mirabilis. The antibacterial activity was studied by disc diffusion method (Anonymous,1996).Methanol extract of Scytonema schmidtii showed the activity against all the tested bacterial strains.Maximum zone of inhibition (23±1.9 mm) was recorded with methanol extract of Scytonema schmidtii.

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Synthesis and Antimicrobial Activity of Piperine Analogues Containing 1,2,4-Triazole Ring

Asian Journal of Chemistry ◽

10.14233/ajchem.2019.21876 ◽

2019 ◽

Vol 31 (5) ◽

pp. 1077-1080

Author(s):

Kottakki Naveen Kumar ◽

Karteek Rao Amperayani ◽

V. Ravi Sankar Ummdi ◽

Uma Devi Parimi

Keyword(s):

Antimicrobial Activity ◽

Triazole Ring ◽

Diffusion Method ◽

Moderate Activity ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Antibacterial Study ◽

Gram Negative ◽

E Coli ◽

Micro Organisms

A series 1,2,4-triazole piperine analogues (TP1-TP6) were designed and synthesized. The structures were confirmed using 1H NMR and 13C NMR. Antibacterial study was done using Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative micro-organisms (E. coli and Pseudomonas aeruginosa) by disc diffusion method. Compound containing chloro substitution (TP6) showed the highest effect, while compound TP1, TP3, TP4, TP5 showed the moderate activity.

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