scholarly journals ASSOCIATION OF NOVEL POLYMORPHISM OF ESR2 GENE (G40100A) WITH PRODUCTION TRAITS OF BROWN LOCAL IRAQI CHICKEN

2021 ◽  
Vol 52 (6) ◽  
pp. 1408-1416
Author(s):  
R.. I. A. Abu-Rekaiba ◽  
W. M. Razuki ◽  
E. H. AL-Anbari
Keyword(s):  
Estrogen Receptor ◽  
Egg Production ◽  
Production Performance ◽  
The Novel ◽  
Production Traits ◽  
Chi Square ◽  
Hardy Weinberg Equilibrium ◽  
Chi Square Analysis ◽  
Esr2 Gene ◽  
Selection Of

The estrogen receptor 2 (ESR2) plays a crucial role in the growth and development of follicles and ovulation in chickens. The aim of this study was detect the association of estrogen receptor 2(ESR2) gene polymorphisms with the production performance of brown local Iraqi chicken. A total of 104 hens from the F4th generation of local chicken selected for high egg yield were used. The novel SNP(G40100A) transition within third prime untranslated region (3 prime UTR) of ESR2 gene. Three genotypes were found: Wild(GG), Heterozygous(GA), and Homozygous(AA) through (PCR-DAN sequencing). Chi-square analysis showed no significant  in genotypic and allelic frequencies for SNP which were agreement with Hardy-Weinberg equilibrium. The genotypes GG and GA were significantly superior  with age at sexual maturity compared to AA genotype. The genotype GA was significantly associated with hen day egg production (HDEP) at 30 weeks compared to genotype AA, as well as the genotype GA  highest significant variance was reported comparing with the GG genotype in HDEP at 45 and 60 weeks, thus, the study recommends to exploit this ESR2 gene polymorphism (G40100A) as promising marker in breeding program via, the selection of individuals carrying the genotype GA, characterized by the high egg production.

scholarly journals Polimorphic Explore of Esr1, Esr2 and Foxl2 Genes and Interaction Effect of Esri and Foxl2 with Productive Traits of Brown Local Iraqi Chickens

2021 ◽  
Vol 910 (1) ◽  
pp. 012004
Author(s):  
Raed Ibraheem A. Abu-Rekaiba ◽  
W. M. Razuki ◽  
E. H. Al-Anbari
Keyword(s):  
Estrogen Receptor ◽  
Production Performance ◽  
Production Traits ◽  
Chi Square ◽  
Beneficial Effects ◽  
Egg Formation ◽  
Hardy Weinberg Equilibrium ◽  
Productive Traits ◽  
Chi Square Analysis ◽  
And Function

Abstract The estrogen receptor 1(ESR1) and estrogen receptor 2 (ESR2) plays a crucial role in the growth and development of follicles and ovulation in chickens. Fork-head box L2 (FOX L2) gene and its protein is active in several tissues, such as the ovaries, it has a pivotal role in ovarian development and function, like regulation of egg formation in poultry. This study aimed to detect the polymorphism of ESR1, ESR2 and, FOXL2 genes and investigate interaction between ESR1 and FOXL2 in the productive performance of brown local Iraqi chickens (BLIC). A total of 104 BLIC, at 17 weeks of age. Four novel single nucleotide polymorphism (SNPs). Every polymorphism a number had been given according to its location within gene, one of them in ESR1 (T57198C) intron 3 with three genotypes: Wild(TT), Heterozygous(TC),and Homozygous(CC), and two SNPs within ESR2 were assessed, one of the identified SNPsT39565C transition located within intron 7 with two alleles(T and C)and three genotypes were found: Wild(TT), Heterozygous(TC),and Homozygous(CC), another SNPG40100A transition within (3 prime UTR), with two alleles (G and A)and three genotypes were found: Wild(GG), Heterozygous(GA)and Homozygous(AA) and the SNP in FOXL2 (C919T) gene with two genotypes: Wild(CC) and Heterozygous(CT) within encoding region were identified through PCR-DNA sequencing. An interaction between the ESR1 and FOXL2 genes has been proposed, and six haplotypes (H1=TTCC, H2=TTCT, H3=TCCC, H4=TCCT, H5=CCCC, H6=CCCT) were obtained due to interaction between these two SNPs. Chi-square analysis showed no significant in genotypic and allelic frequencies for each SNP which revealed those genes were agreement with Hardy-Weinberg equilibrium. Association analysis of haplotypes with production traits revealed that individuals have H4 genotype achieved higher body weight at sexual maturity, at 60 weeks of age and egg weight at 45 and 60 weeks of age, whereas, the higher number of eggs were exhibited to individual with H6 from onset egg till 60 weeks of age. The two genotypes; TCCT and CCCT showed better combination than others with respect to production performance. In conclusion, our findings provided new evidence that the two SNPs (ESR1 and FOXLl2) with special interaction may have potential effects on productive traits of chickens and beneficial effects in laying breeding programs.

scholarly journals POLIMORPHIC CHARACTERIZATION OF ESR1 AND FOXL2 GENES AND THE ASSOCIATION OF THEIR INTERACTION WITH PRODUCTIVE TRAITS OF BROWN LOCAL IRAQI CHICKENS

2021 ◽  
Vol 52 (6) ◽  
pp. 1391-1400
Author(s):  
R. I. A. Abu-Rekaiba ◽  
E. H. AL-Anbari ◽  
W. M. Razuki
Keyword(s):  
Production Performance ◽  
Production Traits ◽  
Chi Square ◽  
Beneficial Effects ◽  
Egg Formation ◽  
Hardy Weinberg Equilibrium ◽  
Productive Traits ◽  
Chi Square Analysis ◽  
New Evidence

The estrogen receptor 1(ESR1) and fork-head box L2 (FOX L2) genes play a pivotal role in regulation of egg formation in poultry. this study aimed to investigate interaction between ESR1 and FOXL2 in the productive performance of brown local Iraqi chickens (BLIC). A total of 104 BLIC represented from the F4th generation of local chicken selected for high egg productions were used. Two novel single nucleotide polymorphism (SNPs), one of them in ESR1 (T57198C) intron 3, and another of FOX L2 (C919T) gene within encoding region were identified through PCR-DNA sequencing. Six haplotypes (H1=TTCC, H2=TTCT, H3=TCCC, H4=TCCT, H5=CCCC, H6=CCCT) were obtained due to interaction between these two SNPs. Chi-square analysis showed no significant  in genotypic and allelic frequencies for each SNP which revealed that both genes were agreement with Hardy-Weinberg equilibrium. Association analysis of haplotypes with production traits revealed that individuals have H4 genotype achieved higher body weight at sexual maturity, at 60 weeks of age and egg weight at 45 and 60 weeks of age, whereas, the higher number of eggs were exhibited in individual with H6 from onset egg till 60 weeks of age. The two haplotypes; H4(TCCT) and H6(CCCT) showed better combination than others with respect to production performance. In conclusion, our findings provided new evidence that the two genes (ESR1 and FOXLl2) with special interaction may have potential effects on productive traits of chickens and beneficial effects in laying breeding programs.

Femoral head to lower lumbar neural foramen distance as a novel radiographic parameter to predict postoperative stretch neuropraxia

2021 ◽  
pp. 1-9
Author(s):  
Alex S. Ha ◽  
Meghan Cerpa ◽  
Justin Mathew ◽  
Paul Park ◽  
Joseph M. Lombardi ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Adult Spinal Deformity ◽  
The Novel ◽  
Continuous Variables ◽  
Curve Correction ◽  
Neural Foramen ◽  
Chi Square ◽  
Chi Square Analysis ◽  
Lower Lumbar ◽  
The Relationship

OBJECTIVE Lumbosacral fractional curves in adult spinal deformity (ASD) patients often have sharp coronal curves resulting in significant pain and imbalance. Postoperative stretch neuropraxia after fractional curve correction can lead to discomfort and unsatisfactory outcomes. The goal of this study was to use radiographic measures to increase understanding of the relationship between postoperative stretch neuropraxia and fractional curve correction. METHODS In 62 ASD patients treated from 2015 to 2018, radiographic review was performed, including measurement of the distance between the lower lumbar neural foramen (L4 and L5) in the concavity and convexity of the lumbosacral fractional curve and the ipsilateral femoral heads (FHs; L4–FH and L5–FH) in pre- and postoperative anteroposterior spine radiographs. The largest absolute preoperative to postoperative change in distance between the lower lumbar neural foramen and the ipsilateral FH (ΔL4/L5–FH) was used for analysis. Chi-square analyses, independent and paired t-tests, and logistic regression were performed to study the relationship between L4/L5–FH and stretch neuropraxia for categorical and continuous variables, respectively. RESULTS Of the 62 patients, 13 (21.0%) had postoperative stretch neuropraxia. Patients without postoperative stretch neuropraxia had an average ΔL4–FH distance of 16.2 mm compared to patients with stretch neuropraxia, who had an average ΔL4–FH distance of 31.5 mm (p < 0.01). Patients without postoperative neuropraxia had an average ΔL5–FH distance of 11.1 mm compared to those with stretch neuropraxia, who had an average ΔL5–FH distance of 23.0 mm (p < 0.01). Chi-square analysis showed that patients had a 4.78-fold risk of developing stretch neuropraxia with ΔL4–FH > 20 mm (95% CI 1.3–17.3) and a 5.17-fold risk of developing stretch neuropraxia with ΔL5–FH > 15 mm (95% CI 1.4–18.7). Logistic regression analysis indicated that the odds of developing stretch neuropraxia were 15:1 with a ΔL4–FH > 20 mm (95% CI 3–78) and 21:1 with a ΔL5–FH > 15 mm (95% CI 4–113). CONCLUSIONS The novel ΔL4/L5–FH distances are strongly associated with postoperative stretch neuropraxia in ASD patients. A ΔL4–FH > 20 mm and ΔL5–FH > 15 mm significantly increase the odds for patients to develop postoperative stretch neuropraxia.

scholarly journals ANALYSIS OF PRODUCTION PERFORMANCE AND FINANCIAL FEASIBILITY OF LAYING DUCK FARM WITH INTENSIVE SYSTEM

2021 ◽  
Vol 24 (1) ◽  
pp. 13
Author(s):  
ANNISA H. N. ◽  
I W. SUKANATA ◽  
B. R. T. PUTRI
Keyword(s):  
Sensitivity Analysis ◽  
Egg Production ◽  
Financial Analysis ◽  
Production Performance ◽  
Primary Data ◽  
Financial Feasibility ◽  
Hatching Eggs ◽  
Break Even Point ◽  
Selection Of ◽  
Intensive System

This study aims to determine the management, production performance, financial feasibility, break-even point and sensitivity of business. The study began from December 2019 until January 2020 at UD. Sari Utama, located in Cepaka Village, Kediri District, Tabanan Regency. This research uses primary data. The variables observed in this study are livestock management, production performance, cost, financial feasibility, and sensitivity. The fe- asibility of laying duck business is analyzed using investment criteria analysis, PBP, BEP and sensitivity analysis. The results of this study indicate that the UD Sari Utama laying duck business has paid attention to several aspects of management including the selection of pra-layer ducks, cages, feed, labor, prevention and treatment of disease. The average egg production is 65%/day and the average number of eggs produced is consumption eggs 62,4% and 37,6% hatching eggs with a maintenance ratio of male and female ducks 1:10. The results of financial analysis show that this farm produces NPV 246.749.932, IRR 26,16%, Net B / C 2,2, PBP in 1,17 years, BEP in 5,96 years, BEP egg production 217.935 / year, BEP egg price Rp 1.649,84 / item. The results of the sensitivity analysis show that the business is sensitive to decreasing egg prices and increasing feed prices, with sensitivity values of 10,52% and 16,87%, respectively. Based on these results it can be concluded that the business of laying duck UD. Sari Utama is financially feasible.

scholarly journals Genetic parameters for egg production traits in crosses between local and exotic chickens estimated by Bayesian inference

2021 ◽  
Vol 44 (3) ◽  
pp. 1-8
Author(s):  
I. Udeh ◽  
S. I. Omeje
Keyword(s):  
Egg Production ◽  
Genetic Parameters ◽  
Genetic Correlations ◽  
Breeding Value ◽  
Appreciable Amount ◽  
Production Traits ◽  
Base Population ◽  
Egg Weight ◽  
Highest Posterior Density ◽  
Selection Of

Estimates of genetic parameters for economic traits are important to enable the breeder estimate the breeding value of individuals available for selection. Thus, this study was carried out to estimate genetic parameters namely heritability and genetic correlations among egg production traits in two strain crosses using bivariate animal model in Bayesian (MCMC) method. The egg production data were obtained from four generations which comprised the base population of two commercial egg strains and the local chicken, the inbred population derived from the base population, the F1 crossbred population obtained from the crossing of the local and exotic inbred chickens and the backcross population obtained from the backcrossing of the crossbred to their parents. A total number of 1,138 daughters of 62 sires and 620 dams were used for the study. The autocorrelations among samples in the MCMC chain were less than 0.1 for all lag values indicating that all samples of the posterior distribution were independent. The estimated heritability for age at first egg, body weight at first egg, hen day egg number, weight of first egg, egg weight at thirty week and egg weight at forty week were 0.62, 0.48, 0.47, 0.53, 0.54 and 0.56 for strain 1 crosses and 0.43, 0.48, 0.49, 0.52, 0.52 and 0.53 for strain 2 crosses. The corresponding highest posterior density interval ranged from 0.22 to 0.91 for strain 1 crosses and 0.07 to 0.83 for strain 2 crosses. The genetic correlations among egg production traits ranged from 0.06 to 1.97 in strain 1 and 0.06 to 2.59 in strain 2 crosses. The estimates were within the range reported in literature for egg production traits in chicken and imply that appreciable amount of additive genes exist in the strain crosses which could be used for the selection of superior birds. The magnitude of genetic correlations implies that selection of one trait could lead to correlated response to the other traits.

Correlated response to selection for some egg performance traits in egg line of Japanese quail

2016 ◽  
Vol 56 (5) ◽  
pp. 913 ◽  
Author(s):  
B. Y. F. Mahmoud ◽  
G. Abou Khadiga ◽  
E. A. El-Full
Keyword(s):  
Japanese Quail ◽  
Egg Production ◽  
Genetic Correlations ◽  
Production Performance ◽  
Egg Mass ◽  
Correlated Response ◽  
Selection Experiment ◽  
Production Traits ◽  
Response To Selection ◽  
Female Line

Estimation of correlated response to selection in female line of Japanese quail after a short-term selection experiment was conducted. The total number of 547 female (275 selected and 272 control) Japanese quail included in the selection experiment of selection depended on aggregate breeding values based on animal model Best Linear Unbiased Prediction. Correlated selection response estimates were favourable for all the studied traits and significant (–0.38, –9.89, –12.86, –16.64, –5.08 and –8.24) for first egg weight (FEW), age at the first 10 eggs (AGE10), age at first 30 eggs (AGE30), age at first 60 eggs (AGE60), days needed to produce the first 30 eggs (DN30) and the first 60 eggs (DN60), respectively. Estimates of heritability were moderate for egg mass for the first 10 eggs (EM10), the first 30 eggs (EM30), the first 60 eggs (EM60), AGE10 and AGE30 (0.17–0.30), but low for AGE60, DN30 and DN60 (0.01–0.10). Genetic correlations between the selection criteria traits (AFE, BWSM and DN10) and the studied egg production traits were positive and ranged from 0.25 to 0.97, 0.24 to 0.95 and 0.06 to 0.98 for AFE, BWSM and DN10, respectively, whereas phenotypic correlations ranged from 0.12 to 0.89, 0.01 to 0.34 and 0.06 to 0.87 for the same traits. The favourable genetic response obtained for egg traits could be an indicator for improving egg production performance in Japanese quail.

scholarly journals Factors Associated with the Selection of Contraceptive Implants for Family Planning Acceptors in Jambi City

2021 ◽  
Vol 7 (2) ◽  
pp. 189-195
Author(s):  
Enny Susilawati ◽  
Neny Heryani ◽  
Lia Artikasari ◽  
Erni Pransiska
Keyword(s):  
Family Planning ◽  
Health Center ◽  
Hormonal Contraceptive ◽  
P Value ◽  
Chi Square ◽  
Cross Sectional ◽  
Significance Level ◽  
Contraceptive Implants ◽  
Chi Square Analysis ◽  
Selection Of

In several decades, based on the results of the census, Indonesia has experienced significant population growth. In the last ten years, the population has increased by 32.56 million. The KB I program was initiated to reduce the birth rate by using several methods, one of which is the hormonal contraceptive implant method. However, data from the Jambi health office shows that there are still quite a few family planning acceptors who use implant contraceptives, even though it is known that this type of contraception is very effective, with a long duration of use, and an affordable price. This study aims to analyze what factors are related to the choice of implant type contraceptives for family planning acceptors in the work area of ​​Putri Ayu Health Center, Jambi City. This type of research is analytic Observational with a cross sectional design. The population of this study was 3,029 people. Samples were taken by quota sampling as many as 96 respondents. Data collection was carried out using a questionnaire. Data analysis was carried out bivariately using the chi square test with a significance level of 0.05. The data collected is based on research variables, namely knowledge, attitudes, perceptions, and motivations of family planning acceptors. The results of Chi Square analysis showed that knowledge with p-value 0.002, attitude p-value 0.224, perception p-value 0.173, and motivation with p-value 0.005. Factors related to the selection of contraceptive implants for mothers of family planning acceptors in the working area of ​​Putri Ayu Health Center are knowledge and motivation  

scholarly journals Confirmation of Alleles Inheritance in F1 Progenies Derived from a Cross of Calcutta-4 [Musa acuminata ssp. burmannicoides] and Musa acuminata ssp. microcarpa Based on SSR Markers

2020 ◽  
Vol 16 (1) ◽  
pp. 17
Author(s):  
Dea Rosalia ◽  
Puji Lestari ◽  
Andy Soegianto ◽  
Darmawan Saptadi ◽  
Agus Sutanto ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Disease Resistance ◽  
Ssr Markers ◽  
Selection Process ◽  
Musa Acuminata ◽  
Chi Square ◽  
Genetic Studies ◽  
Ssr Primers ◽  
Chi Square Analysis ◽  
Selection Of

<p>Banana breeding to produce improved varieties with disease resistance haracters and other desired traits could sustain its yield. Alleles harbored by parents could be passed on to the offsprings through hybridization, but need to be confirmed using molecular markers. This study aimed to confirm allele inheritance in F1 progenies derived from a cross of Calcutta-4 (Musa acuminata ssp.  burmannicoides) and M. acuminata ssp. microcarpa based on SSR markers. Eleven pairs of SSR primers were used to amplify DNA of 44 progenies using the PCR technique. The results showed that six SSR markers (MaSSR 1.1, MaSSR<br />5.1, MaSSR 6.1, MaSSR 7.1, MaSSR 8.1, and MaSSR 11.1) were polymorphic in both parents. Four markers (MaSSR 1.1, MaSSR 5.1, MaSSR 6.1, and MaSSR 8.1) had PIC &gt;0.7, indicating their informativeness to distinguish these progenies and other genetic studies of banana germplasms. A total of 44 F1 individuals were confirmed to harbor alleles inherited from their parents,<br />suggesting as true progenies from the cross of Calcutta-4 and M. acuminata ssp. microcarpa. This population demonstrated 100% success of hybridization performed. Chi-Square analysis revealed that segregation of all markers did not match to Mendelian ratio 1:2:1, except for MaSSR 1.1 (x2 = 5,62) and MaSSR 6.1 (x2 = 3,77) markers. The genetic traceability of banana F1 progenies demonstrating the usefulness and feasibility of SSR markers in this study provided information on selection of true progenies which may be valuable for breeders to assist selection process in future banana breeding program in Indonesia.</p>

scholarly journals Heterosis for egg production in native by exotic by inbred chicken crosses

2021 ◽  
Vol 32 (1) ◽  
pp. 7-20
Author(s):  
I. Udeh ◽  
S. I. Omeje
Keyword(s):  
Egg Production ◽  
Production Performance ◽  
Egg Mass ◽  
Production Traits ◽  
Short Term ◽  
Gene Effects ◽  
Egg Weight ◽  
Native Chicken ◽  
Backcross Populations ◽  
Dominant Genes

Inbred lines generated from the native and exotic chickens were compared with their F1 and backcross populations for a range of short term egg production traits. The experimental birds were brooded for 8 weeks and raised on a deep litter pens from 8 to 40 weeks of age. Age at first eggs were longer in the crossbred groups and the delay was mostly caused by the dominant genes from the exotic parents. Highly significant (P>0.01) heterosis were obtained by the crossbred groups in hen day rate and total egg mass respectively. The heterosis observed in the crossbred groups for egg weight was low and mostly non significant. the backcross groups recorded residual heterosis in egg production traits ( hen day rate, total egg mass and egg weight) and the magnitude was higher in the exotic than the native backcrosses. The results further indicate that the egg production performance of the crossbred groups were mostly influenced by maternal, sex linked, dominance and epistatic gene effects. It was suggested that the egg production potential of the native chicken could be improved further by cross mating-selected native and exotic backcross groups. This should be followed by crisscrossing.

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