Pilot Production of Bacterial Cellulose by Gluconacetobacter hansenii TL-2C

2007 ◽  
Vol 36 (10) ◽  
pp. 1341-1350
Author(s):  
Ji-Suk Jeong ◽  
Jong-Sun Kim ◽  
Kyoung-Ho Choi
Keyword(s):  
Bacterial Cellulose ◽  
Gluconacetobacter Hansenii

scholarly journals Evaluation of Different Methods for Cultivating Gluconacetobacter hansenii for Bacterial Cellulose and Montmorillonite Biocomposite Production: Wound-Dressing Applications

Polymers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 267 ◽  
Author(s):  
Katharine Valéria Saraiva Hodel ◽  
Larissa Moraes dos Santos Fonseca ◽  
Isa Moreira da Silva Santos ◽  
Jamile Costa Cerqueira ◽  
Raimundo Evangelista dos Santos-Júnior ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Wound Dressing ◽  
Industrial Applications ◽  
Medical Product ◽  
Water Holding Capacity ◽  
Mechanical And Thermal Properties ◽  
Water Release ◽  
Thermal Stabilities ◽  
Gluconacetobacter Hansenii ◽  
Water Holding

Bacterial cellulose (BC) has received considerable attention due to its unique properties, including an ultrafine network structure with high purity, mechanical strength, inherent biodegradability, biocompatibility, high water-holding capacity and high crystallinity. These properties allow BC to be used in biomedical and industrial applications, such as medical product. This research investigated the production of BC by Gluconacetobacter hansenii ATCC 23769 using different carbon sources (glucose, mannitol, sucrose and xylose) at two different concentrations (25 and 50 g∙L−1). The BC produced was used to develop a biocomposite with montmorillonite (MMT), a clay mineral that possesses interesting characteristics for enhancing BC physical-chemical properties, at 0.5, 1, 2 and 3% concentrations. The resulting biocomposites were characterized in terms of their physical and barrier properties, morphologies, water-uptake capacities, and thermal stabilities. Our results show that bacteria presented higher BC yields in media with higher glucose concentrations (50 g∙L−1) after a 14-day incubation period. Additionally, the incorporation of MMT significantly improved the mechanical and thermal properties of the BC membranes. The degradation temperature of the composites was extended, and a decrease in the water holding capacity (WHC) and an improvement in the water release rate (WRR) were noted. Determining a cost-effective medium for the production of BC and the characterization of the produced composites are extremely important for the biomedical applications of BC, such as in wound dressing materials.

scholarly journals Complete Genome Sequence of aGluconacetobacter hanseniiATCC 23769 Isolate, AY201, Producer of Bacterial Cellulose and Important Model Organism for the Study of Cellulose Biosynthesis

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Sarah Pfeffer ◽  
Kalpa Mehta ◽  
R. Malcolm Brown
Keyword(s):  
Nucleotide Sequence ◽  
Bacterial Cellulose ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Nucleotide Sequence ◽  
Complete Genome ◽  
Model Organism ◽  
Cellulose Biosynthesis ◽  
Gluconacetobacter Hansenii

The cellulose producer and model organism used for the study of cellulose biosynthesis,Gluconacetobacter hanseniiAY201, is a variant ofG. hanseniiATCC 23769. We report here the complete nucleotide sequence ofG. hanseniiAY201, information which may be utilized to further the research into understanding the genes necessary for cellulose biosynthesis.

scholarly journals Physical Characteristics and Cell-Adhesive Properties of In Vivo Fabricated Hyaluronan/Bacterial Cellulose Nanocomposites

2021 ◽  
Author(s):  
Ryo Takahama ◽  
Honami Kato ◽  
Go Takayama ◽  
Kenji Tajima ◽  
Tetsuo Kondo
Keyword(s):  
Bacterial Cellulose ◽  
Physical Characteristics ◽  
Genetically Engineered ◽  
Basic Material ◽  
Adhesive Properties ◽  
Gluconacetobacter Hansenii ◽  
Modification Technique ◽  
Cellulose Nanocomposites

Abstract This study attempts to clarify the basic material properties of in-vivo-fabricated hyaluronan (HA)/bacterial cellulose (BC) nanocomposites prepared previously. BC membranes (pellicles) generated by Gluconacetobacter hansenii (G. hansenii) are promising biomaterials owing to their outstanding biocompatible properties. Recently, specific demands for biomedical applications of BC have increased owing to its excellent mechanical properties. Although many techniques have been developed to improve the biofunctional properties of BC pellicles, such modifications remain limited owing to technical difficulties in the modulation of complex biosynthetic processes. Therefore, we previously developed an in vivo modification technique to produce nanocomposite pellicles composed of BC and HA (in vivo HA/BC), which are directly secreted from genetically engineered G. hansenii. In the present study, the HA extractability and content rate, physical characteristics, and cytocompatibility of in vivo HA/BC have been investigated in comparison to conventional in situ HA/BC and native BC pellicle. The results suggested that HA more strongly adsorbed to the solid BC surface of in vivo HA/BC than that of in situ HA/BC, which possibly affected the dynamic viscoelastic characteristics. Furthermore, in vivo HA/BC showed remarkably high human epidermal cell adhesion. These results indicate the great potential of in vivo modification to expand the usefulness of BC-based biomaterials.

Sign in / Sign up

Export Citation Format

Share Document