scholarly journals Selectivity of Metarhizium anisopliae and Beauveria bassiana to adults of Telenomus podisi (Hymenoptera: Scelionidae)

2022 ◽  
Vol 43 (2) ◽  
pp. 727-738
Author(s):  
Lucas Battisti ◽  
◽  
Jheniffer Valmira Warmling ◽  
Claudinei de Freitas Vieira ◽  
Darlin Henrique Ramos de Oliveira ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Metarhizium Anisopliae ◽  
Tube Surface ◽  
Biological Control Agents ◽  
Adult Females ◽  
Inner Surface ◽  
Glass Tubes ◽  
Telenomus Podisi ◽  
And Control ◽  
Beneficial Organisms

The selectivity of entomopathogenic fungi to non-target organisms needs to be considered in Integrated Pest Management (IPM), because even though they are biological control agents, if used incorrectly, they can negatively alter the functioning of agroecosystems. Therefore, studies that assess the selectivity of these fungi to beneficial organisms are extremely important. The objective of this work was to evaluate the selectivity of Metarhizium anisopliae (Metarril®) and Beauveria bassiana (Boveril®) to adults of Telenomus podisi Ashmead (Hymenoptera: Scelionidae), under laboratory conditions. The products were evaluated on adult females of T. podisi, at the concentrations recommended by the manufacturer. To this, 0.2 mL of suspensions of each product and control (treatments) were applied to the inner surface of glass tubes, and then a female T. podisi was placed in it (≤ 48 h of emergence). After 24 h of contact, cards with 25 eggs of Euschistus heros Fabricius (Hemiptera: Pentatomidae) were offered for 24 h (COF24). After this period, the COF24 were withdrawn. After 72 h of contact of the female with the tube surface, new E. heros egg cards were made available (COF72) for 24 h for T. podisi ovipositioning. The mortality of T. podisi females was evaluated daily to determine longevity, percentage of parasitism and emergence, sex ratio, and egg-adult period of the T. podisi offspring. Metarril® and Boveril®, considered selective for adult females of T. podisi, did not negatively affect most of the parameters evaluated.

scholarly journals Comparative Virulence of Metarhizium anisopliae and Four Strains of Beauveria bassiana Against House Fly (Diptera: Muscidae) Adults With Attempted Selection for Faster Mortality

2021 ◽  
Author(s):  
Roxie L White ◽  
Christopher J Geden ◽  
Phillip E Kaufman ◽  
Dana Johnson
Keyword(s):  
Beauveria Bassiana ◽  
Metarhizium Anisopliae ◽  
House Fly ◽  
The Other ◽  
Biological Control Agents ◽  
High Concentration ◽  
House Flies ◽  
Lethal Time ◽  
Selection For ◽  
Time Required

Abstract Entomopathogenic fungi such as Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae/brunneum (Metchnikoff)/Petch have shown promising results for managing the house fly, Musca domestica L. A primary challenge of using these biological control agents (BCAs) in field situations is the time required to induce high adult house fly mortality, typically 6–7 d post-exposure. In this study, virulence of M. anisopliae (strain F52) and four B. bassiana strains were compared. The B. bassiana strains GHA and HF23 are used in commercial products and those were compared with two strains that were isolated from house flies on dairy farms (NFH10 and L90). Assays were conducted by exposing adult house flies to fungal-treated filter paper disks for 2 h. The lethal time to 50% mortality (LT50) at the high concentration of 1 × 109 conidia ranged from 3.8 to 5.2 d for all five strains. GHA, NFH10, and L90 killed flies faster than M. anisopliae strain F52; HF23 did not differ from either the M. anisopliae or the other B. bassiana strains. Attempts with the NFH10 strain to induce faster fly mortality through selection across 10 fungal to fly passages did not result in shorter time to fly death of the selected strain compared with the unselected strain.

scholarly journals Pathogenicity of commercial entomopathogenic fungal strains on the avocado seed borer (ASB), Heilipus lauri (Coleoptera: Curculionidae) under laboratory conditions

2020 ◽  
Vol 40 (4) ◽  
pp. 1059-1067 ◽  
Author(s):  
Andrea P. Clavijo ◽  
Claudia M. Holguin
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Metarhizium Anisopliae ◽  
Fold Increase ◽  
Fruit Intake ◽  
Initiation Time ◽  
Biological Control Agents ◽  
Direct Inoculation ◽  
Potential Use ◽  
Avocado Seed

Abstract The avocado seed borer, (ASB) Heilipus lauri Boheman 1845 (Coleoptera: Curculionidae) is an official quarantine pest limiting avocado production and fruit exports from Colombia to pest-free countries. To provide alternatives to manage ASB, the objective of this study was to determine the potential use of commercial strains of Beauveria bassiana and Metarhizium anisopliae as biological control agents of this pest. Pathogenicity of the strains was evaluated by complete immersion of the insect in a spore solution (direct inoculation) or by insect contact with inoculated fruit (indirect inoculation). The B. bassiana strain caused higher mortality (DF = 3, F = 25.7, P = 0.0002), showing an almost eight-fold increase, 55% and 79% mortality, for both concentrations used, 2.5 × 108 spores/mL or 2.5 × 109 spores/mL, respectively. Additionally, the B. bassiana strain showed shorter median survivorship using direct inoculation (15.8 to 8.7 days), a longer delay in the feeding initiation time (50 h), and a decrease in the fruit intake (Anti-feeding index above 50) after indirect inoculation. To our knowledge, this is the first report about the pathogenicity of a commercial B. bassiana strain against ASB in Colombia.

scholarly journals Selectivity of pesticides used in rice crop on Telenomus podisi and Trichogramma pretiosum

2016 ◽  
Vol 46 (3) ◽  
pp. 327-335 ◽  
Author(s):  
Juliano de Bastos Pazini ◽  
Anderson Dionei Grützmacher ◽  
José Francisco da Silva Martins ◽  
Rafael Antônio Pasini ◽  
Matheus Rakes
Keyword(s):  
Biological Control ◽  
Metarhizium Anisopliae ◽  
Rice Crop ◽  
Trichogramma Pretiosum ◽  
Biological Control Agents ◽  
Irrigated Rice ◽  
Flooded Rice ◽  
Telenomus Podisi ◽  
Selective Pesticides ◽  
Main Strategy

ABSTRACT Telenomus and Trichogramma species stand out as agents for the biological control in rice crops, and the main strategy for preserving them is the use of selective pesticides. This study aimed at evaluating the toxicity of pesticides used in irrigated rice crop on Telenomus podisi Ashmead (Hymenoptera: Platygastridae) and Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae). Adults of these parasitoids were exposed to dry residues of pesticides, in a completely randomized experiment, with 25 treatments (24 pesticides + control) and four replications. The insecticides clorantraniliprole, flubendiamide and diflubenzuron and the biological insecticides based on Beauveria bassiana and Metarhizium anisopliae were harmless to T. podisi and T. pretiosum. The harmless herbicides were: 2.4-D amine, profoxydim, quinclorac, ethoxysulfuron and saflufenacil. The fungicide epoxiconazole + kresoxim-methyl was also harmless to these two biological control agents. Therefore, these pesticides are indicated for the integrated pest management, in flooded rice areas.

scholarly journals Pathogenicity of indigenous strains of three entomopathogenic fungi to the sisal weevil, Scyphophorus acupunctatus (Gyllenhal) (Coleoptera: Curculionidae)

2015 ◽  
Vol 8 (2) ◽  
pp. 46-54
Author(s):  
V.T. Gkounti ◽  
D. Markoyiannaki ◽  
D.Ch. Kontodimas
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Fungal Species ◽  
Life Stages ◽  
Biological Control Agents ◽  
Isaria Fumosorosea ◽  
Mean Survival Time ◽  
High Pathogenicity

SummaryThe pathogenicity of indigenous isolates of Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea was evaluated in the laboratory against larvae and adults of the sisal weevil, Scyphophorus acupunctatus. Inoculation was achieved via immersion of individuals into conidia suspensions of different concentrations. All three fungal species proved high pathogenicity against larvae of the weevil, causing 100% mortality in most of the treatments. Beauveria bassiana caused the highest mortality of the adults (86.67±12%), followed by M. anisopliae (46.67±17.8%) and I. fumosorosea (40±17.5%). Mean survival time also differed significantly among treatments and life stages of the weevil. In total, larvae survived significantly fewer days than adults post infection. Results of the present study indicate the potential of indigenous strains of entomopathogenic fungi as biological control agents against the invasive weevil.

Evaluación de hongos entomopatógenos para el control biológico del mosquito Culex quinquefasciatus

2009 ◽  
Vol 5 (9) ◽  
pp. 25-38
Author(s):  
Martha Giselle Rivera Pineda ◽  
Sandra Patricia Garzón Lozano ◽  
Luz Inés Villarreal Salazar
Keyword(s):  
Beauveria Bassiana ◽  
Culex Quinquefasciatus ◽  
Metarhizium Anisopliae

En busca de una alternativa de control del mosquito Culex quinquefasciatus en áreas urbanas se desarrolló el proyecto: “Evaluación de microorganismos biocontroladores sobre el mosquito Culex quinquefasciatus” cuyo objetivo fue evaluar la capacidad biocontroladora de diferentes hongos entomopatógenos sobre larvas del mosquito a nivel de laboratorio. Se evaluaron 16 aislamientos de hongos en diferentes concentraciones. Se constataron diferencias en el porcentaje de mortalidad producido en larvas de Cx. quinquefasciatus dependientes del tipo de aislamiento, de la concentración utilizada y del tiempo de exposición. Los aislamientos que presentaron el nivel de mortalidad más alto pertenecen a la especie Metarhizium anisopliae, seguidos por aislamientos de la especie Beauveria bassiana. El aislamiento que presentó el mayor nivel de mortalidad (100%) en menor tiempo de exposición (24 horas) fue Metarhizium anisopliae 99068 en una concentración de 1x106 e/ml. Por lo tanto este aislamiento es recomendado para su evaluación en campo y desarrollo de un producto comercial.

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