scholarly journals THE EFFECTS OF PARAFFIN AND PARAFILM APPLICATIONS AND DIFFERENT ROOTSTOCKS ON YIELD OF GRAFTED VINE IN ‘BLACK MAGIC’ GRAPE CULTIVAR

AGROFOR ◽  
2021 ◽  
Vol 4 (2) ◽  
Author(s):  
Önder KAMİLOĞLU ◽  
Özge DEMİRKESER ◽  
Nihat SAKAROĞLU
Keyword(s):  
Callus Formation ◽  
Formation Rate ◽  
Shoot Length ◽  
The Other ◽  
Bud Burst ◽  
Grape Cultivar ◽  
Black Magic

The scions belonging to 'Black Magic' grape cultivar were grafted on ‘41B’, ‘1103 P’, ‘Fercal’ and ‘SO4’ American rootstock cuttings using omega grafting method in the experiment. Paraffin was applied to one half of the grafted cuttings. The other half was wrapped with parafilm and then paraffin was applied. Grafted cuttings were planted in perlite medium at 3.0 x 3.5 cm intervals inside plastic buckets and kept in the stratification room for 4 weeks. Grafted cuttings, which had been maintained under room conditions for one week, were then grown for two months under unheated greenhouse conditions. In order to determine rootstock and application effects, bud burst ratio (%), callus formation rate (%), callus formation degree (0-4), rooting ratio (%), rooting degree (0-4), shoot length (cm), shoot diameter (mm) and grafted vine ratio (%) were examined. According to the results of the study, it was determined that paraffin+parafilm application yielded better results than paraffin application in terms of bud burst ratio (respectively 70.83%, 53.33%), callus formation ratio (respectively 83.75%, 69.58%) and yield of grafted-vines (respectively 68.33%, 51.67%). In terms of the rootstocks, bud burst ratio was determined to be the highest in SO4 rootstock (71.67%) and the lowest in Fercal (54.17%). 1103 P rootstock yielded a highest value than other rootstocks in terms of shoot length (26.17 cm), callus rate at grafting surface (88.33%), and rooting ratio (97.50%). The effect of rootstocks on shoot diameter, rooting degree, and grafted vine ratio was found to be similar.

Temperature Effects during Endodormancy Induction on Subsequent Anthesis and Growth of Peach Trees

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 452c-452 ◽  
Author(s):  
Schuyler D. Seeley ◽  
Raymundo Rojas-Martinez ◽  
James Frisby
Keyword(s):  
Temperature Effects ◽  
Shoot Length ◽  
The Other ◽  
Nighttime Temperatures ◽  
Peach Trees ◽  
Better Than

Mature peach trees in pots were treated with nighttime temperatures of –3, 6, 12, and 18 °C for 16 h and a daytime temperature of 20 °C for 8 h until the leaves abscised in the colder treatments. The trees were then chilled at 6 °C for 40 to 70 days. Trees were removed from chilling at 40, 50, 60, and 70 days and placed in a 20 °C greenhouse under increasing daylength, spring conditions. Anthesis was faster and shoot length increased with longer chilling treatments. Trees exposed to –3 °C pretreatment flowered and grew best with 40 days of chilling. However, they did not flower faster or grow better than the other treatments with longer chilling times. There was no difference in flowering or growth between the 6 and 12 °C pretreatments. The 18 °C pretreatment resulted in slower flowering and very little growth after 40 and 50 days of chilling, but growth was comparable to other treatments after 70 days of chilling.

scholarly journals التفسير العلمي لأحاديث التمر في ضوء جمع روايات الحديث والاكتشافات العلمية الجديدة

2018 ◽  
Vol 10 (11) ◽  
Author(s):  
Yusuf Ismail
Keyword(s):  
Scientific Information ◽  
Arabic Language ◽  
The Other ◽  
Other Hand ◽  
Black Magic

The Hadiths of the Prophet regarding benefits of dates or scientifically known as Phoenix dactilyfera has gain so much attention from the scientist of Islamic knowledge and the modern sciences. Most researches presented in the topic focus on the benefit of dates in dealing with poisoning as it were clearly stated in the Hadith, furthermore some new scientific findings prove that the benefits of dates are far beyond that. Besides, the benefits of dates in dealing with black magic is still undiscovered by the modern sciences, even though some people claim to explain it scientifically, it is no more than an example of pseudoscience. It is also important to mention that most of the books published in the field of al-‘Ijaz al-‘ilmi related to the dates issue, especially those written in Arabic language has been observed to repeat the same scientific information regarding the benefits of dates despite the presence of new scientific researches and findings. On the other hand, some researches in this subject also has been notably neglect the importance of collecting all related texts of the Hadith before starting to interpret it, which is a vital step in understanding the Hadith. Therefore this research aims at understanding the Hadith regarding the benefits of dates through collecting all related Hadith in the topic and further interpreting it scientifically by referring to new scientific researches available.

scholarly journals Comparative analyses of stevioside between fresh leaves and in-vitro derived callus tissue from Stevia rebaudiana Bert. using HPLC

2015 ◽  
Vol 49 (4) ◽  
pp. 199-204 ◽  
Author(s):  
S Mahmud ◽  
S Akter ◽  
IA Jahan ◽  
S Khan ◽  
A Khaleque ◽  
...  
Keyword(s):  
Retention Time ◽  
Callus Tissue ◽  
Callus Formation ◽  
Leaf Explants ◽  
Stevia Rebaudiana ◽  
Poor Performance ◽  
The Other ◽  
Ms Medium ◽  
Green Callus

A protocol was developed to produce large amount of callus in short a period of time from leaf explants of Stevia rebaudiana Bert. The highest amount of white callus was obtained on MS medium supplemented with 2.5 mg/l 2, 4-D and 0.5 mg/l BAP after 3 weeks of inoculating leaf segments. On the other hand, 0.5 mg/l BAP and 1.0 mg/l Kn exhibits poor performance towards callus formation while after using 1.0 mg/l Kn alone did not develop any callus. In this experiment, highest amount of green callus was obtained when MS medium supplemented with 2.5 mg/l NAA and 10% coconut water was used. An improved analytical method HPLC was applied to analyze stevioside extracted from the leaf and callus of Stevia rebaudiana. The stevioside in each sample were analyzed by comparing their retention times with those of the standards. The retention time (RT) of stevioside for leaves were found 14.96 and for callus 13.81 mins. The percentage of stevioside content from leaves and callus was 12.19% and 12.62% respectively DOI: http://dx.doi.org/10.3329/bjsir.v49i4.22621 Bangladesh J. Sci. Ind. Res. 49(4), 199-204, 2014

67 Effects of phytohemagglutinin on the culture of isolated bovine blastomeres derived from the 8-cell stage invitro-produced embryos

2020 ◽  
Vol 32 (2) ◽  
pp. 159
Author(s):  
Y. Hashiyada ◽  
Y. Aikawa ◽  
H. Matsuda ◽  
T. Yamanouchi
Keyword(s):  
Tissue Culture ◽  
Culture Medium ◽  
Flat Surface ◽  
Formation Rate ◽  
Cell Number ◽  
The Other ◽  
Blastocyst Formation ◽  
Culture Dish ◽  
Cell Stage ◽  
Tissue Culture Dish

Monozygotic twin embryos which can efficiently be produced by blastomere separation and aggregation of early cleavage stages of embryos using commercially provided well-of-the-well (WOW) culture dish. Phytohaemagglutinin (PHA) is a plant lectin that binds to and aggregates on the surface of animal cells, but also contains toxicity that causes food poisoning. The present study was conducted to evaluate the toxicity to embryos and the effect to development of isolated blastomeres on PHA-supplemented WOW culture. Embryos were produced using oocytes from ovaries collected at an abattoir by IVM, IVF, and invitro culture (IVC). The tissue culture medium 199 supplemented with 5% calf serum (CS), Brackett-Oliphant solution supplemented with 10mgmL−1 bovine serum albumin, and CR1aa medium containing 5% CS were used for each culture step. For the evaluation of PHA toxicity, 89 embryos that developed to the 5-8-cell stage were obtained at Day 2 after insemination. Each embryo was cultured in a droplet of 5 µL/embryo IVC culture medium supplemented with or without PHA. For the evaluation of PHA to development of isolated blastomeres, 111 of 8-cell stage embryos were obtained 48-54h post-insemination. Zonae pellucidae were removed by exposure to 0.25% pronase. Then, embryos were separated into single blastomeres by gentle pipetting in IVC medium. Each four blastomeres were formed in the shape of a bunch inside the thin cylinder at the tip of the Pasteur pipette by gentle pipetting. Then, each mass of blastomeres in each 60 masses was cultured individually in 5-µL droplets of IVC medium supplemented with or without PHA on the flat surface of a tissue culture dish. On the other hand, each four blastomeres were introduced into a single conical micro-well each having a diameter and depth of ~287µm and 168µm (Dai Nippon Printing). This culture of blastomeres was performed covered with a droplet of 2.5µL well−1 IVC medium supplemented with or without PHA in each 50 or 52 wells. In all of investigations, PHA was used at 50µgmL−1 (Akagi et al. 2011 J. Reprod. Dev. 57). Statistical analysis was performed using Student's t-test and analysis of variance. The blastocyst formation rate (71.1±2.3% vs. 72.7±1.7%), total cell number (120 vs. 122), and inner cell mass cell number (47 vs. 51) at Day 7 after IVF did not differ between PHA-supplemented and PHA-free group in the toxicity test, respectively. In the blastomere culture, the blastocyst formation rate was very low (10.0±5.9% vs. 5.0±2.9%) regardless of the PHA supplementation in drops on the flat surface of a tissue culture dish. On the other hand, blastocyst formation was improved using the WOW culture dish (24.0±3.6% vs. 40.4±7.6%) but there was no difference with or without PHA supplementation. Although nontoxicity of PHA and efficacy of WOW culture for isolated-aggregated blastomeres were confirmed, no improvement of PHA supplementation on development was observed in this study. Subsequently, experiments on the optimum concentration of PHA for aggregation and development of blastomeres in WOW culture are required.

scholarly journals Response of growth and antioxidant enzymes to osmotic stress in two different wheat (Triticum aestivum L.) cultivars seedlings

2012 ◽  
Vol 58 (No. 12) ◽  
pp. 534-539 ◽  
Author(s):  
G.Q. Wu ◽  
L.N. Zhang ◽  
Y.Y. Wang
Keyword(s):  
Triticum Aestivum ◽  
Antioxidant Enzymes ◽  
Osmotic Stress ◽  
Wheat Cultivar ◽  
Triticum Aestivum L ◽  
Shoot Length ◽  
The Other ◽  
Wheat Cultivars ◽  
Wheat Seedlings ◽  
Drought Tolerant

 To investigate the responses of growth and antioxidant enzymes to osmotic stress in two different wheat cultivars, one drought tolerant (Heshangtou, HST) and the other drought sensitive (Longchun 15, LC15), 15-day-old wheat seedlings were exposed to osmotic stress of –0.25, –0.50, and –0.75 MPa for 2 days. It is found that osmotic stress decreased shoot length in both wheat cultivars, whereas to a lesser degree in HST than in LC15. The contents of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) of shoot in both wheat cultivars were increased by osmotic stress. It is clear that MDA contents increased less in the more drought tolerant cultivar HST than in drought sensitive one LC15. On the contrary, POD and CAT activities increased more in HST than LC15 under osmotic stress. As the activity of SOD, however, no significant differences were found between HST and LC15. These results suggest that wheat cultivar HST has higher activities of antioxidant enzymes such as POD and CAT to cope with oxidative damage caused by osmotic stress compared to sensitive LC15.  

Plant growth and fruit yield attributes of cape gooseberry cv. Aligarh as affected by the use of different growth regulators

2016 ◽  
Vol 36 (2) ◽  
Author(s):  
Gurpinder Kaur ◽  
Amarjeet Kaur
Keyword(s):  
Growth Regulators ◽  
Fruit Set ◽  
Shoot Length ◽  
The Other ◽  
Maturation Time ◽  
Internodal Length ◽  
Yield Attributes ◽  
And Control ◽  
Cape Gooseberry ◽  
Growth Characters

The present investigation was carried out in the Departmental nursery of Khalsa College, Amritsar during year 2013-14. It is comprised of seven treatments as NAA (5, 10 and 15 ppm), GA<sub>3</sub> (10, 15 and 20 ppm) and control which were replicated four times. A unit of eight plants per plot considered as one replication. The growth regulators were applied immediately after fruit set. Both the growth regulators were found to be effective in improving the growth characters as plant height, stem thickness, average shoot length, shoot thickness, internodal length and average leaf area than control. GA<sub>3</sub> 20 ppm proved to be most effective in improving the growth characters than all the other treatments. Fruit set per cent (71.40%) found to be maximum in plants which were treated with NAA 15 ppm whereas the plants sprayed with GA<sub>3</sub> 10 ppm yielded maximum fruits (1.58 kg/plant). Fruits showed rich colour and the maturation time (175.30 days) was delayed in those plants which were sprayed with GA<sub>3</sub> 20 ppm.

Study on Tissue Culture of Sweet Broad Pea

2014 ◽  
Vol 644-650 ◽  
pp. 5407-5410
Author(s):  
Hui Fang Chi
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Culture Medium ◽  
Callus Formation ◽  
Formation Rate ◽  
Adventitious Bud ◽  
Experimental Basis ◽  
Research Results

s. The cotyledons, Internodes, leaves and stems of sweet broad pea were studied on tissue culture. Research results show that: The ability of different explants for callus formation and adventitious bud differentiation in different culture medium is different. The callus formation rate and sprouting rate of Internodes is significantly higher than other explants, which is a ideal material for tissue culture. The callus formation rate of Internodes was 100% in MS +BA1.0 mg/L+NAA 1.0 mg/L and MS+ 2, 4-D 0.5 mg/L; The bud differentiation is best at the medium of MS+ 6-BA 2 mg/L, which reached 86.7%; the rooting rate was 83.3% at the medium of MS+ NAA 3mg/L. The study provides a experimental basis for further study on the plant regeneration in the sweet broad pea.

scholarly journals Response of in vitro propagated fig (Ficus carica L.) shoots to the concentrations of benzyl amino purine and coconut water

2021 ◽  
Vol 922 (1) ◽  
pp. 012067
Author(s):  
Sophia ◽  
M Hayati ◽  
E Kesumawati
Keyword(s):  
Callus Formation ◽  
Formation Rate ◽  
Water Concentration ◽  
Shoot Formation ◽  
Coconut Water ◽  
Contamination Rate ◽  
Two Factors ◽  
Number Of Leaves ◽  
Benzyl Amino Purine

Abstract In this study, several concentrations of benzyl amino purine (BAP) and coconut water (CW) were investigated along with the interaction between two factors to the growth of in vitro propagated fig shoots. The investigated factors consisted of BAP concentration: 0, 1, 3, 5 mg L−1 and coconut water concentration: 0, 100, 200, 300 ml L−1. A total of 16 treatment combinations with 6 replications resulting in 96 experimental units consisting of a single fig shoot explant per culture medium. The observed parameters including living explant rate, contamination rate, browning rate, day of first shoot emergence, shoot formation rate, explant height addition, number of leaves, callus formation rate, and number of roots were conducted every week from 1 to 8 weeks after proliferation (WAP). The result indicated that in 8 WAP, the living explant rate reached 23.95%. The combination of concentration 200 ml L−1 CW and 3 mg L−1 BAP + 200 ml L−1 CW-induced early emergence of new shoots at 7 days after proliferation (DAP). The highest shoot formation rate (100%) was observed at a concentration of 300 mL L−1CW. The highest explant height addition (7.10 cm) was observed at a concentration of 200 mL L−1 CW. The highest number of leaves (5.80) was observed at a concentration of 1 mg L−1 BAP + 200 mL L−1 CW. The highest callus formation rate (50%) was observed at a concentration of 100 ml L−1CW and 300 ml L−1 CW. The highest number of roots (17) was observed in the control.

scholarly journals 606 In Vitro Propagation of Some Strawberry Cultivars in Response to Kind and Concentration of Some Cytokinin Compounds

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 501D-501
Author(s):  
M.H. Aboul-Nasr ◽  
S.Z. Elagamy ◽  
A.M. Kassim
Keyword(s):  
In Vitro Propagation ◽  
Callus Formation ◽  
Shoot Length ◽  
Control Treatment ◽  
Fresh Weight ◽  
Shoot Height ◽  
Murashige And Skoog Medium ◽  
Number Of Leaves ◽  
Strawberry Cultivars

Three strawberry cultivars were used in this study. Runners from the three cultivars were grown on Murashige and Skoog medium supplemented with benzylaminpurine or kinetin at four concentrations (2, 1, 0.5, and 0.25 mg·L-1 in addition to the control treatment). The use of cytokinin, regardless of type and concentration, promoted the explant proliferation, shoot height, number of leaves, and plantlet fresh weight. Callus formation was enhanced by BA application, especially with `Pajaro'. However, `Chandler' did not form at all using cytokinin. Produced plantlets had lower nutrient constituents (N, P, K, Ca, and Na) when grown on media containing cytokinin compared to the control. Based on these results, cytokinin is recommended to get more shoots per explant. Furthermore, using the lowest concentration (0.25 mg·L-1) will produce height proliferation, greater shoot length, and more leaves.

scholarly journals Pair lines of sight observations of multiphase gas bearing O vi in a galaxy environment

2021 ◽  
Vol 503 (3) ◽  
pp. 3243-3261
Author(s):  
Pratyush Anshul ◽  
Anand Narayanan ◽  
Sowgat Muzahid ◽  
Alexander Beckett ◽  
Simon L Morris
Keyword(s):  
Column Density ◽  
Dwarf Galaxy ◽  
Formation Rate ◽  
Angular Separation ◽  
The Other ◽  
Chemical Abundances ◽  
Lower Column ◽  
Solar Metallicity ◽  
Galaxy Environment ◽  
Collisional Ionization

ABSTRACT Using HST/COS spectra of the twin quasar lines of sight Q 0107–025A & Q 0107–025B, we report on the physical properties, chemical abundances, and transverse sizes of a multiphase medium in a galaxy field at z = 0.399. The angular separation between the quasars corresponds to a physical separation of 520 kpc at the absorber redshift. The absorber towards Q 0107–025B is a partial Lyman limit system (pLLS) with $\log N({\mathrm{H}}{\small I})/\hbox{cm$^{-2}$}\approx 16.8$. The H i column density in the absorber along the other sightline is ≈ 2 orders of magnitude lower. The O vi along both sightlines have comparable column densities and broad b-values (b &gt; 30 km s−1) whereas the low ionization lines are considerably narrower. The low ionization gas is inconsistent with the O vi when modelled assuming photoionization from the same phase. In both lines of sight, O vi and the broad H i coinciding, are best explained through collisional ionization in a cooling plasma with solar metallicity. Ionization models infer 1/10th solar metallicity for the pLLS and solar metallicity for the lower column density absorber along the other sightline. Within ± 250 km s−1 and 2 Mpc of projected distance from the sightlines 12 galaxies are identified, of which five are within 500 kpc. The twin sightlines are at normalized impact parameters of ρ ∼ 1.1Rvir, and ρ ∼ 0.8Rvir from a M* ∼ 1010.7 M⊙, L ∼ 0.07L*, and star formation rate (SFR) &lt; 0.1 M⊙ yr−1 galaxy, potentially probing its CGM (circumgalactic medium). The next closest in normalized separation are a dwarf galaxy with M* ∼ 108.7 M⊙, and SFR ∼ 0.06 M⊙ yr−1, and an intermediate mass galaxy with M* ∼ 1010.0 M⊙, and SFR ∼ 3 M⊙ yr−1. Along both sightlines, O vi could be either tracing narrow transition temperature zones at the interface of low ionization gas and the hot halo of nearest galaxy, or a more spread-out warm component that could be gas bound to the circumgalactic halo or the intragroup medium. The latter scenarios lead to a warm gas mass limit of M ≳ 4.5 × 109 M⊙.

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