scholarly journals Antibody capture process based on magnetic beads from very high cell density suspension

2021 ◽  
Author(s):  
Nils A. Brechmann ◽  
Hubert Schwarz ◽  
Per‐Olov Eriksson ◽  
Kristofer Eriksson ◽  
Atefeh Shokri ◽  
...  
Keyword(s):  
Cell Density ◽  
Magnetic Beads ◽  
High Cell Density ◽  
High Cell ◽  
Capture Process ◽  
Antibody Capture ◽  
Very High

Preparation of Very-High-Yield Recombinant Proteins Using Novel High-Cell-Density Bacterial Expression Methods

2010 ◽  
Vol 2010 (8) ◽  
pp. pdb.prot5475 ◽  
Author(s):  
Victoria Murray ◽  
Jianglei Chen ◽  
Yuefei Huang ◽  
Qianqian Li ◽  
Jianjun Wang
Keyword(s):  
Cell Density ◽  
Recombinant Proteins ◽  
High Cell Density ◽  
Bacterial Expression ◽  
High Yield ◽  
High Cell ◽  
Very High

scholarly journals Very high cell density perfusion of CHO cells anchored in a non-woven matrix-based bioreactor

2015 ◽  
Vol 213 ◽  
pp. 28-41 ◽  
Author(s):  
Ye Zhang ◽  
Per Stobbe ◽  
Christian Orrego Silvander ◽  
Véronique Chotteau
Keyword(s):  
Cell Density ◽  
Cho Cells ◽  
High Cell Density ◽  
High Cell ◽  
Very High

scholarly journals pH AND POPULATION DENSITY IN THE REGULATION OF ANIMAL CELL MULTIPLICATION

1971 ◽  
Vol 51 (3) ◽  
pp. 686-702 ◽  
Author(s):  
H. Rubin
Keyword(s):  
Cell Migration ◽  
Cell Density ◽  
Plasma Membranes ◽  
High Cell Density ◽  
Low Ph ◽  
Cell Multiplication ◽  
High Cell ◽  
High Ph ◽  
Cell Densities ◽  
Very High

Sparse and dense cultures of chick embryo cells were affected differently by pH. The rates of cell multiplication and of thymidine-3H incorporation into DNA of dense cultures were increased as the pH was increased from 6.6 to 7.6. At pH higher than 7.6 the rate of multiplication decreased slightly in the dense cultures, but the rate of thymidine-3H incorporation continued to increase. The discrepancy was due in part to cell death and detachment at very high pH, and in part to a more rapid uptake of thymidine-3H at very high pH. Sparse cultures were much less sensitive to pH reduction and, when a suitably conditioned medium was used to minimize cell damage, very sparse cultures grew almost as well at pH 6.7 as at higher pH. The rates of cell multiplication and thymidine-3H incorporation at low pH decreased in the initially sparse cultures before they reached confluent cell densities. There was no microscope evidence of direct contact between plasma membranes of cells at these densities although the parallel orientation indicated that the cells were influencing locally each other's behavior. Even at much higher cell densities, electron microscopy revealed large intercellular gaps partly filled with a fragmentary electron-opaque material suspected to be glycoprotein. Wounding experiments showed that pH affected cell migration in a manner similar to its effects on cell multiplication. Low pH inhibited cell migration, but those cells which migrated into the denuded region multiplied as rapidly at low pH as at high pH. The effects of pH on growth were correlated with effects on the uptake of 2-deoxyglucose-3H. Dense populations of cells inhibited by low pH were stimulated to incorporate thymidine-3H by the addition of small amounts of diethylaminoethyl-dextran. Rous sarcoma cells at high cell density were less sensitive to pH than were normal cells at the same density, but were more sensitive than sparse normal cultures. The results suggest that cell growth is inhibited through the combined effects of both lowered pH and high cell density on cell surface permeability.

scholarly journals Explore the activation efficiency of different ligand carriers on synNotch-based contact-dependent activation system

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Zhiyuan Niu ◽  
Guanxu Chen ◽  
Han Chen ◽  
Meichen Yin ◽  
Lingtong Zhi ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Density ◽  
Magnetic Beads ◽  
High Cell Density ◽  
Receptor Activation ◽  
High Cell ◽  
Receptor Ligands ◽  
Red Fluorescence ◽  
Activation Efficiency ◽  
Activation System

AbstractObjectivessynNotch receptors can get different inputs about the outside environment into cells; however, synNotch system doesn’t work for soluble ligands. This study aims to explore the activation efficiency of different ligand carriers on synNotch system.MethodsSynNotch-based contact-dependent activation system was constructed in 293T cells (293T-synNotch), then the synNotch receptor ligands eGFP proteins were provided by three different carriers: 400 nm Ni magnetic beads (Mag Ni-eGFP), 300 nm carboxyl modified Fe3O4 magnetic beads (Mag COOH-deGFP), and 4T1 cell (4T1-mGFP). Three eGFP carriers were incubated with 293T-synNotch, the activation efficiencies of these three eGFP carriers were evaluated by fluorescence microscopy.ResultsThe 293T-synNotch cell activated by Mag Ni-eGFP shows no observable red fluorescence; when Mag COOH-deGFP are incubated with 293T-synNotch, about 50‰ cells with red fluorescence appeared, also eGFP proteins have not dropped off from the Mag COOH-deGFP beads; 4T1-mGFP cells show the highest activation efficiency, about 21% synNotch cells are activated, when 4T1-mGFP and 293T-synNotch are incubated at high cell density.ConclusionThe 4T1-mGFP cells are the most effective ligands for synNotch receptor activation.

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