scholarly journals Glial cell line-derived neurotrophic factor and chronic electrical stimulation prevent VIII cranial nerve degeneration following denervation

2002 ◽  
Vol 454 (3) ◽  
pp. 350-360 ◽  
Author(s):  
Sho Kanzaki ◽  
Timo Stöver ◽  
Kohei Kawamoto ◽  
Diane M. Prieskorn ◽  
Richard A. Altschuler ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Cell Line ◽  
Glial Cell ◽  
Neurotrophic Factor ◽  
Cranial Nerve ◽  
Nerve Degeneration ◽  
Chronic Electrical Stimulation

scholarly journals Can Early Electrical Stimulation Accelerates the Neural Regeneration by Increasing the Expression of BDNF and GDNF in Distal Part of Injured Peripheral Nerve? An Animal Experimental Study

2021 ◽  
Vol 9 (A) ◽  
pp. 1006-1010
Author(s):  
Agus Roy Rusly Hariantana Hamid ◽  
Sri Maliawan ◽  
DPG Purwa Samatra ◽  
I Nyoman Mantik Astawa ◽  
I Made Bakta ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Sciatic Nerve ◽  
Cell Line ◽  
Glial Cell ◽  
Neurotrophic Factors ◽  
Neurotrophic Factor ◽  
Distal Part ◽  
Neural Regeneration ◽  
Control Group ◽  
Distal Nerve

BACKGROUND: The role of neurotrophic factors (brain-derived neurotrophic factors and glial cell line-derived neurotrophic factors) and early electrical stimulation (EES) in the injured nerve has found promising in several studies. However, there is still limited knowledge about the effect of EES in the distal part of the nerve to sustain this level of expression of growth factors. AIM: We aim to evaluate the effects of EES in in neural regeneration by measuring the expression of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) in animal model. METHODS: The research was conducted starting from April to May 2021 using male Wistar rats. Using general anesthesia, the sciatic nerve was cut. The intervention group was treated with EES in the distal stump, right after nerve resection (20 Hz, 1–2 mA, 2–5 s), while the control group received no treatment after nerve resection. A reoperation on day 3 was performed in both groups to measure BDNF and GDNF expression level of the distal nerve tissue by ELISA as well as histopathological examination of sprouting axons of the injured proximal nerve. RESULTS: A total of 32 samples were included in the study. A statistically significant levels of GDNF is found higher in the EES group (n = 16) than the control group (n = 16) (35. 71 pg/100 mg, confidence interval (CI) 95% 23.93, 47.48, p < 0.05). The number of sprouting axons is found lower in the EES group (p < 0.05). The BDNF level is similar between the two groups, however not significant. After a subgroup analysis, it was found that the greater the level of GDNF, the fewer the axon sprouts in both groups (fewer axon group 58.35 [n = 22, CI 95% 45.14, 71.55] vs. more axon group 47.14 [n = 10, CI 95% 35.33, 58.95]), p < 0.05. CONCLUSION: The EES proves its benefit in accelerating the axonal regeneration by increasing the expression GDNF in the distal nerve stumps in the electrical excited degenerated sciatic nerve in the rat model.

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