scholarly journals Developmental rescue of an embryonic-lethal mutation in the retinoblastoma gene in chimeric mice.

1994 ◽  
Vol 13 (18) ◽  
pp. 4260-4268 ◽  
Author(s):  
E.C. Maandag ◽  
M. van der Valk ◽  
M. Vlaar ◽  
C. Feltkamp ◽  
J. O'Brien ◽  
...  
Keyword(s):  
Lethal Mutation ◽  
Retinoblastoma Gene ◽  
Chimeric Mice ◽  
Embryonic Lethal

scholarly journals Three ENU-induced alleles of the murine quaking locus are recessive embryonic lethal mutations

1988 ◽  
Vol 51 (2) ◽  
pp. 95-102 ◽  
Author(s):  
Monica J. Justice ◽  
Vernon C. Bode
Keyword(s):  
Mouse Chromosome ◽  
Lethal Mutation ◽  
Chromosome 17 ◽  
Wild Type ◽  
Embryonic Survival ◽  
Lethal Mutations ◽  
Embryonic Lethal ◽  
Embryonic Lethal Mutations ◽  
Precise Time ◽  
New Alleles

SummaryThe quaking (qk) locus on mouse chromosome 17 has been defined by a single viable quaking allele. Three new alleles of quaking were selected after ENU mutagenesis by their failure to complement the quaking phenotype. The qkk2 allele was induced on wild-type chromatin and the qkkt1 and qkkt4 alleles were induced on t-chromatin. Each is a recessive embryonic lethal mutation. They fail to complement each other and are not complemented by the deletion, TtOrl. Homozygotes and hemizygotes die at 8–9·5 days gestation, but not at a single precise time. Because the classical quaking mutation complements the lethality of these new alleles, but they fail to complement its quaking phenotype (myelination defect), we conclude that the quaking+ function is required for embryonic survival as well as for myelination.

scholarly journals Generation of chimeric mice with spermatozoa fully derived from embryonic stem cells using a triple-target CRISPR method for Nanos3†

2020 ◽  
Author(s):  
Kento Miura ◽  
Shogo Matoba ◽  
Michiko Hirose ◽  
Atsuo Ogura
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Embryonic Stem ◽  
Conditional Knockout ◽  
Mouse Strains ◽  
Chimeric Mice ◽  
Cell Functions ◽  
Embryonic Lethal ◽  
Lethal Genes

Abstract Conditional knockout (cKO) mice have contributed greatly to understanding the tissue- or stage-specific functions of genes in vivo. However, the current cKO method requires considerable time and effort because of the need to generate two gene-modified mouse strains (Cre transgenic and loxP knockin) for crossing. Here, we examined whether we could analyze the germ cell-related functions of embryonic lethal genes in F0 chimeric mice by restricting the origin of germ cells to mutant embryonic stem cells (ESCs). We confirmed that the full ESC origin of spermatozoa in fertile chimeric mice was achieved by the CRISPR/Cas9 system using three guide RNAs targeting Nanos3, which induced germ cell depletion in the host blastocyst-derived tissues. Among these fertile chimeric mice, those from male ESCs with a Dnmt3b mutation, which normally causes embryo death, also produced F1 mice derived exclusively from the mutant ESCs. Thus, our new chimeric strategy readily revealed that Dnmt3b is dispensable for male germ cell development, in agreement with a previous cKO study. Our new approach enables us to analyze the germ cell functions of embryonic lethal genes in the F0 generation without using the current cKO method.

Inserted retroviruses cause embryonic lethal mutation

Nature ◽  
1982 ◽  
Vol 300 (5891) ◽  
pp. 401-402 ◽  
Author(s):  
Keith R. Willison
Keyword(s):  
Lethal Mutation ◽  
Embryonic Lethal

Ectrodactyly: A New Embryonic Lethal Mutation in the Chicken

1966 ◽  
Vol 57 (5) ◽  
pp. 207-211 ◽  
Author(s):  
U. K. ABBOTT ◽  
J. A. MACCABE
Keyword(s):  
Lethal Mutation ◽  
Embryonic Lethal

Recessive lethal mutation induced in the mouse by chronic γ -irradiation

1957 ◽  
Vol 147 (928) ◽  
pp. 402-411 ◽  
Keyword(s):  
Lethal Mutation ◽  
Desoxyribonucleic Acid ◽  
Γ Irradiation ◽  
Recessive Lethal ◽  
Mouse Sperm ◽  
Recessive Lethal Mutation ◽  
Embryonic Lethal ◽  
Radiation Induced ◽  
Average Size ◽  
Lethal Genes

When the sons of irradiated mice are mated to their own daughters, the average size of the ensuing litters will depend on the number of recessive embryonic lethal genes segregating; it can therefore be used to estimate the rate of induction of recessive lethal mutation by the radiation, provided that one can show that any change is not due to dominant sublethals or semisteriles. Data from an experiment in which mouse sperm were exposed to low accumu­lated doses of radium γ -radiation lead to an estimate of one recessive lethal per 300 roentgen. This is 23 times the corresponding rate for Drosophila melanogaster . It may be compared with the results reported by Russell (1952), who found that the rate of induction of recessive visibles per locus in mouse spermatogonia is 15 times the Drosophila rate; and with those of Vendrely (1955), who found that mouse nuclei have 29 times as much desoxyribonucleic acid as Drosophila nuclei. It is suggested that the mouse may have only slightly more genes than Drosophila , but that each may be about 19 times as large and, for this reason, about 19 times as sensitive to radiation-induced mutation. Implications for human radiogenetic studies are discussed.

scholarly journals Stumpy Limb—An Embryonic Lethal Mutation in Japanese Quail (Coturnix coturnix japonica)

1991 ◽  
Vol 40 (4) ◽  
pp. 529-536 ◽  
Author(s):  
Masaoki TSUDZUKI ◽  
Noboru WAKASUGI ◽  
Hiroshi MORIOKA ◽  
Kozaburo ESAKI
Keyword(s):  
Japanese Quail ◽  
Lethal Mutation ◽  
Coturnix Japonica ◽  
Coturnix Coturnix Japonica ◽  
Coturnix Coturnix ◽  
Embryonic Lethal
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