Differential expression of osteogenic factors associated with osteoinductivity of human osteosarcoma cell lines

2004 ◽  
Vol 70A (1) ◽  
pp. 122-128 ◽  
Yan Yu ◽  
Richard I. Harris ◽  
Jia-Lin Yang ◽  
H. Clarke Anderson ◽  
William R. Walsh
2008 ◽  
Vol 103 (3) ◽  
pp. 994-1004 ◽  
Alenka Kovacevic ◽  
Astrid Hammer ◽  
Elke Stadelmeyer ◽  
Werner Windischhofer ◽  
Monika Sundl ◽  

2001 ◽  
Vol 383 ◽  
pp. 259-267 ◽  
Bennett S. Burns ◽  
Matthew L. Edin ◽  
Gayle E. Lester ◽  
Harrison G. Tuttle ◽  
Monroe E. Wall ◽  

1989 ◽  
Vol 35 (2) ◽  
pp. 223-229 ◽  
J R Farley ◽  
E Kyeyune-Nyombi ◽  
N M Tarbaux ◽  
S L Hall ◽  
D D Strong

Abstract Earlier we described a kinetic assay for quantifying skeletal alkaline phosphatase (ALP) isoenzyme activity in serum. The precision of the assay depends on including ALP standards for the skeletal, hepatic, intestinal, and placental isoenzymes. We wondered whether human osteosarcoma cells could provide an efficient alternative to human bone or Pagetic serum as a source of the skeletal ALP standard. ALP activities prepared from five human osteosarcoma cell lines were compared with a bone-derived ALP standard with respect to heat stability and sensitivity to chemical effectors. Two of the cell lines (SaOS-2 and TE-85) contained ALP activities that resembled the bone-derived standard. We selected SaOS-2 cells for additional evaluation (as a potential source of isoenzyme standard), because they contained 40-50 times more ALP activity than did the TE-85 cells. To include the SaOS-2 cell-derived ALP activity in the quantitative isoenzyme assay, we diluted the enzyme in a solution containing heat-inactivated (i.e., ALP-negative) human serum. Surprisingly, this dilution caused a 60-125% increase in maximum enzyme activity. In the quantitative assay of ALP isoenzyme in serum, the SaOS-2 derived ALP was indistinguishable from the serum skeletal ALP standard, with respect to the above criteria and assay variations. Evidently ALP from SaOS-2 cells is suited as a standard for measuring skeletal ALP activity in this assay.

1996 ◽  
Vol 5 ◽  
pp. 103-104

2020 ◽  
Vol 35 (8) ◽  
pp. 879-887 ◽  
Yung‐Chi Lin ◽  
Hsuan‐Ying Chen ◽  
Cheng‐Pu Hsieh ◽  
Yi‐Fu Huang ◽  
Ing‐Lin Chang

Sarcoma ◽  
2007 ◽  
Vol 2007 ◽  
pp. 1-5 ◽  
Jendrik Hardes ◽  
Arne Streitburger ◽  
Helmut Ahrens ◽  
Thomas Nusselt ◽  
Carsten Gebert ◽  

Purpose. The antimicrobial effect of a silver-coated tumor endoprosthesis has been proven in clinical and experimental trials. However, in the literature there are no reports concerning the effect of elementary silver on osteoblast behaviour. Therefore, the prosthetic stem was not silver-coated because of concerns regarding a possible inhibition of the osseointegration. The aim of the present study was to investigate the effect of 5–25 mg of elementary silver in comparison to Ti-6Al-4V on human osteosarcoma cell lines (HOS- 58, SAOS).Methods. Cell viability was determined by measuring the MTT proliferation rate. Cell function was studied by measuring alkaline phosphatase (AP) activity and osteocalcine production.Results. In the HOS-58 cells, the AP activity was statistically significant(P<0.05)higher at a supplement of 5–10 mg of silver than of Ti-6 Al-4V at the same doses. For both cell lines, a supplement above 10 mg of silver resulted in a reduced AP activity in comparision to the Ti-6 Al-4V group, but a statistically significant difference(P<0.05)was observed at a dose of 25 mg for the SAOS cells only. At doses of 20–25 mg in the HOS-58 cells and 10–25 mg in the SAOS cells, the reduction of the proliferation rate by silver was statistically significant(P<0.05)compared to the Ti-6 Al-4V supplement.Discussion. In conclusion, elementary silver exhibits no cytotoxicity at low concentrations. In contrast, it seems to be superior to Ti-6 Al-4V concerning the stimulation of osteogenic maturation at these concentrations, whereas at higher doses it causes the known cytotoxic properties.

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