Superparamagnetic iron oxide nanoparticles change endothelial cell morphology and mechanics via reactive oxygen species formation

2010 ◽  
Vol 96A (1) ◽  
pp. 186-195 ◽  
Author(s):  
Kivilcim Buyukhatipoglu ◽  
Alisa Morss Clyne
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cell ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cell Morphology ◽  
Superparamagnetic Iron Oxide ◽  
Oxide Nanoparticles ◽  
Oxygen Species ◽  
Reactive Oxygen Species Formation ◽  
Species Formation

scholarly journals Activatable Superparamagnetic Iron Oxide Nanoparticles Scavenge Reactive Oxygen Species in Macrophages and Endothelial Cells

2020 ◽  
Author(s):  
Chukwuazam Nwasike ◽  
Eunsoo Yoo ◽  
Erin Purr ◽  
Amber L. Doiron
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Iron Oxide ◽  
Contrast Agent ◽  
Iron Oxide Nanoparticles ◽  
Reactive Oxygen ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Oxygen Species

<p>This study centers around diagnostic medicine, and severity staging of inflammatory diseases. Previously, we showed that complexation of PEG and Poly(gallol) on superparamagnetic iron oxide nanoparticles turn OFF the MRI contrasting ability of the nanoparticle. However, in the presence of reactive oxygen species, the contrast agent will turn ON. In this article, for the first time, we provide evidence that our MRI contrast agent is sensitive to physiologically relevant ROS and induces antioxidant activity on immune and endothelial cells. This study provides initial evidence of IPC-SPIOs cellular ROS sensitivity and potential activatable properties in biological conditions.</p>

scholarly journals Activatable Superparamagnetic Iron Oxide Nanoparticles Scavenge Reactive Oxygen Species in Macrophages and Endothelial Cells

2020 ◽  
Author(s):  
Chukwuazam Nwasike ◽  
Eunsoo Yoo ◽  
Erin Purr ◽  
Amber L. Doiron
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Iron Oxide ◽  
Contrast Agent ◽  
Iron Oxide Nanoparticles ◽  
Reactive Oxygen ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Oxygen Species

<p>This study centers around diagnostic medicine, and severity staging of inflammatory diseases. Previously, we showed that complexation of PEG and Poly(gallol) on superparamagnetic iron oxide nanoparticles turn OFF the MRI contrasting ability of the nanoparticle. However, in the presence of reactive oxygen species, the contrast agent will turn ON. In this article, for the first time, we provide evidence that our MRI contrast agent is sensitive to physiologically relevant ROS and induces antioxidant activity on immune and endothelial cells. This study provides initial evidence of IPC-SPIOs cellular ROS sensitivity and potential activatable properties in biological conditions.</p>

scholarly journals Activatable superparamagnetic iron oxide nanoparticles scavenge reactive oxygen species in macrophages and endothelial cells

RSC Advances ◽  
2020 ◽  
Vol 10 (68) ◽  
pp. 41305-41314
Author(s):  
Chukwuazam Nwasike ◽  
Eunsoo Yoo ◽  
Erin Purr ◽  
Amber L. Doiron
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Reactive Oxygen ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Oxygen Species ◽  
Intracellular Ros

Complexed IPC-SPIOs scavenge intracellular ROS after internalization.

scholarly journals Kallistatin attenuates endothelial apoptosis through inhibition of oxidative stress and activation of Akt-eNOS signaling

2010 ◽  
Vol 299 (5) ◽  
pp. H1419-H1427 ◽  
Author(s):  
Bo Shen ◽  
Lin Gao ◽  
Yi-Te Hsu ◽  
Grant Bledsoe ◽  
Makato Hagiwara ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Endothelial Cell ◽  
Oxygen Species ◽  
Phosphatidylinositol 3 Kinase ◽  
Reactive Oxygen Species Formation ◽  
Endothelial Apoptosis ◽  
Species Formation ◽  
Tnf Α

Kallistatin is a regulator of vascular homeostasis capable of controlling a wide spectrum of biological actions in the cardiovascular and renal systems. We previously reported that kallistatin inhibited intracellular reactive oxygen species formation in cultured cardiac and renal cells. The present study was aimed to investigate the role and mechanisms of kallistatin in protection against oxidative stress-induced vascular injury and endothelial cell apoptosis. We found that kallistatin gene delivery significantly attenuated aortic superoxide formation and glomerular capillary loss in hypertensive DOCA-salt rats. In cultured endothelial cells, kallistatin suppressed TNF-α-induced cellular apoptosis, and the effect was blocked by the pharmacological inhibition of phosphatidylinositol 3-kinase and nitric oxide synthase (NOS) and by the knockdown of endothelial NOS (eNOS) expression. The transduction of endothelial cells with adenovirus expressing dominant-negative Akt abolished the protective effect of kallistatin on endothelial apoptosis and caspase activity. In addition, kallistatin inhibited TNF-α-induced reactive oxygen species formation and NADPH oxidase activity, and these effects were attenuated by phosphatidylinositol 3-kinase and NOS inhibition. Kallistatin also prevented the induction of Bim protein and mRNA expression by oxidative stress. Moreover, the downregulation of forkhead box O 1 (FOXO1) and Bim expression suppressed TNF-α-mediated endothelial cell death. Furthermore, the antiapoptotic actions of kallistatin were accompanied by Akt-mediated FOXO1 and eNOS phosphorylation, as well as increased NOS activity. These findings indicate a novel role of kallistatin in the protection against vascular injury and oxidative stress-induced endothelial apoptosis via the activation of Akt-dependent eNOS signaling.

scholarly journals The effect of size and surface ligands of iron oxide nanoparticles on blood compatibility

RSC Advances ◽  
2020 ◽  
Vol 10 (13) ◽  
pp. 7559-7569 ◽  
Author(s):  
Tao Liu ◽  
Ru Bai ◽  
Huige Zhou ◽  
Rongqi Wang ◽  
Jing Liu ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Iron Oxide ◽  
Blood Cell ◽  
Iron Oxide Nanoparticles ◽  
Blood Compatibility ◽  
Vascular Endothelial Cell ◽  
Superparamagnetic Iron Oxide ◽  
Oxide Nanoparticles ◽  
Vascular Endothelial ◽  
Cell Plasma

Due to the unique physicochemical properties, superparamagnetic iron oxide nanoparticles (SPIONs) have attracted increased attention, which show different effects on red blood cell, plasma, platelet, C3 complement and vascular endothelial cell.

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