In vitro gastrointestinal digestion of the major peach allergen Pru p 3, a lipid transfer protein: Molecular characterization of the products and assessment of their IgE binding abilities

2010 ◽  
Vol 54 (10) ◽  
pp. 1452-1457 ◽  
Author(s):  
Valeria Cavatorta ◽  
Stefano Sforza ◽  
Giancarlo Aquino ◽  
Gianni Galaverna ◽  
Arnaldo Dossena ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Lipid Transfer Protein ◽  
Lipid Transfer ◽  
Gastrointestinal Digestion ◽  
Transfer Protein ◽  
Ige Binding ◽  
In Vitro Gastrointestinal Digestion ◽  
Pru P 3

scholarly journals Molecular characterization of pine embryogenesis: pursuing the role of a putative non-specific lipid-transfer protein

2011 ◽  
Vol 5 (Suppl 7) ◽  
pp. P71 ◽  
Author(s):  
Marta Simões ◽  
Andreia Rodrigues ◽  
José de Vega Bartol ◽  
Raissa Santos ◽  
Célia Miguel
Keyword(s):  
Molecular Characterization ◽  
Lipid Transfer Protein ◽  
Lipid Transfer ◽  
Transfer Protein ◽  
Specific Lipid

Characterization of the allergic T-cell response to Pru p 3, the nonspecific lipid transfer protein in peach

2009 ◽  
Vol 124 (1) ◽  
pp. 100-107 ◽  
Author(s):  
Véronique Schulten ◽  
Astrid Radakovics ◽  
Christina Hartz ◽  
Adriano Mari ◽  
Sonia Vazquez-Cortes ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Response ◽  
Lipid Transfer Protein ◽  
T Cell Response ◽  
Lipid Transfer ◽  
Transfer Protein ◽  
Nonspecific Lipid Transfer Protein ◽  
Pru P 3

Molecular characterization of Api g 2, a novel allergenic member of the lipid-transfer protein 1 family from celery stalks

2010 ◽  
Vol 55 (4) ◽  
pp. 568-577 ◽  
Author(s):  
Gabriele Gadermaier ◽  
Matthias Egger ◽  
Tamara Girbl ◽  
Anja Erler ◽  
Andrea Harrer ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Lipid Transfer Protein ◽  
Lipid Transfer ◽  
Transfer Protein

scholarly journals Identification and molecular characterization of allergenic non-specific lipid-transfer protein from durum wheat (Triticum turgidum )

2018 ◽  
Vol 49 (1) ◽  
pp. 120-129 ◽  
Author(s):  
Hela Safi ◽  
Andrea Wangorsch ◽  
Jonas Lidholm ◽  
Faiçal Brini ◽  
Jelena Spiric ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Molecular Characterization ◽  
Lipid Transfer Protein ◽  
Triticum Turgidum ◽  
Lipid Transfer ◽  
Transfer Protein ◽  
Specific Lipid

Purification and characterization of a small (7.3 kDa) putative lipid transfer protein from maize seeds

2003 ◽  
Vol 794 (1) ◽  
pp. 109-114 ◽  
Author(s):  
Mariana S. Castro ◽  
Isabel R. Gerhardt ◽  
Stefania Orrù ◽  
Piero Pucci ◽  
Carlos Bloch
Keyword(s):  
Lipid Transfer Protein ◽  
Lipid Transfer ◽  
Purification And Characterization ◽  
Transfer Protein ◽  
Maize Seeds

scholarly journals The Medicago truncatula N5 Gene Encoding a Root-Specific Lipid Transfer Protein Is Required for the Symbiotic Interaction with Sinorhizobium meliloti

2009 ◽  
Vol 22 (12) ◽  
pp. 1577-1587 ◽  
Author(s):  
Youry Pii ◽  
Alessandra Astegno ◽  
Elisa Peroni ◽  
Massimo Zaccardelli ◽  
Tiziana Pandolfini ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Sinorhizobium Meliloti ◽  
Lipid Transfer Protein ◽  
Symbiotic Association ◽  
Lipid Transfer ◽  
Symbiotic Interaction ◽  
Transgenic Roots ◽  
Transfer Protein ◽  
Small Proteins

The Medicago truncatula N5 gene is induced in roots after Sinorhizobium meliloti infection and it codes for a putative lipid transfer protein (LTP), a family of plant small proteins capable of binding and transferring lipids between membranes in vitro. Various biological roles for plant LTP in vivo have been proposed, including defense against pathogens and modulation of plant development. The aim of this study was to shed light on the role of MtN5 in the symbiotic interaction between M. truncatula and S. meliloti. MtN5 cDNA was cloned and the mature MtN5 protein expressed in Escherichia coli. The lipid binding capacity and antimicrobial activity of the recombinant MtN5 protein were tested in vitro. MtN5 showed the capacity to bind lysophospholipids and to inhibit M. truncatula pathogens and symbiont growth in vitro. Furthermore, MtN5 was upregulated in roots after infection with either the fungal pathogen Fusarium semitectum or the symbiont S. meliloti. Upon S. meliloti infection, MtN5 was induced starting from 1 day after inoculation (dpi). It reached the highest concentration at 3 dpi and it was localized in the mature nodules. MtN5-silenced roots were impaired in nodulation, showing a 50% of reduction in the number of nodules compared with control roots. On the other hand, transgenic roots overexpressing MtN5 developed threefold more nodules with respect to control roots. Here, we demonstrate that MtN5 possesses biochemical features typical of LTP and that it is required for the successful symbiotic association between M. truncatula and S. meliloti.

Molecular cloning and characterization of a lipid transfer protein gene (PsLTP1) from Pinus sylvestris (L.)

2018 ◽  
Vol 30 (3) ◽  
pp. 1149-1158
Author(s):  
Nataliya Hrunyk ◽  
Valentina Kovaleva ◽  
Hryhoriy Krynytskyy ◽  
Ivan Gout ◽  
Francisco Amil-Ruiz ◽  
...  
Keyword(s):  
Pinus Sylvestris ◽  
Molecular Cloning ◽  
Protein Gene ◽  
Lipid Transfer Protein ◽  
Lipid Transfer ◽  
Transfer Protein
Sign in / Sign up

Export Citation Format

Share Document