Age determination of hybrid catfish Ictalurus punctatus ♀ x Ictalurus furcatus ♂ and Channel Catfish Ictalurus punctatus from commercial catfish farms

Author(s):  
James D. Creel ◽  
Terrill R. Hanson ◽  
Luke A. Roy ◽  
Steven M. Sammons
2018 ◽  
Vol 50 (1) ◽  
pp. 78-86 ◽  
Author(s):  
Menghe H. Li ◽  
David J. Wise ◽  
Charles C. Mischke ◽  
Suja Aarattuthodiyil ◽  
Ambika Tiwari ◽  
...  

1999 ◽  
Vol 82 (3) ◽  
pp. 614-619 ◽  
Author(s):  
Steven M Plakas ◽  
Kathleen R El Said ◽  
F Aladar Bencsath ◽  
Steven M Musser ◽  
Calvin C Walker

Abstract Rapid methods are described for determination of flumequine (FLU) residues in muscle and plasma of farm-raised channel catfish (Ictalurus punctatus). FLU residues were extracted from tissues with an acidified methanol solution, and extracts were cleaned up on C18 solid-phase extraction cartridges. FLU concentrations were determined by liquid chromatography (LC)using a C18 analytical column and fluorescence detection (excitation, 325 nm; emission, 360 nm). Mean recoveries of FLU from fortified muscle were 87–94% at 5 levels ranging from 10 to 160 ppb (5 replicates per level). FLU recoveries from fortified plasma were 92–97% at 5 levels ranging from 20 to 320 ppb. Limits of detection (signal-to-noise ratio, 3:1)for the method as described were 3 and 6 ppb for muscle and plasma, respectively. Relative standard deviations (RSDs) for recoveries were ≤12%. Live catfish were dosed with 14C-labeled or unlabeled FLU to generate incurred residues. Recoveries of 14C residues throughout extraction and cleanup were 90 and 94% for muscle and plasma, respectively. RSDs for incurred FLU at 2 levels in muscle and plasma ranged from 2 to 6%. The identity of FLU in incurred tissues was confirmed by LC/mass spectrometry.


2017 ◽  
Vol 49 (5) ◽  
pp. 912-918 ◽  
Author(s):  
Menghe H. Li ◽  
David J. Wise ◽  
Charles C. Mischke ◽  
Ganesh Kumar ◽  
Penelope M. Lucas

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