This paper reports on field studies and model development aimed at understanding coliform fate and transport in the Quabbin Reservoir, an oligotrophic drinking water supply reservoir. An investigation of reservoir currents suggested the importance of wind driven phenomena, and that both lateral and vertical circulation patterns exist. In-situ experiments of coliform decay suggested dependence on light intensity and yielded an appropriate decay coefficient to be used in CE-QUAL-W2, a two-dimensional hydrodynamic and water quality model. Modeling confirmed the sensitivity of reservoir outlet concentration to vertical variability within the reservoir, meteorological conditions, and location of coliform source.