Have men and women evolved sex-distinct mating preferences for short-term and long-term mating, as postulated by some evolutionary theorists? Direct tests of assumptions, consideration of confounds with gender, and examination of the same variables for both sexes suggest men and women are remarkably similar. Furthermore, cross-species comparisons indicate that humans do not evidence mating mechanisms indicative of short-term mating (e.g., large female sexual skins, large testicles). Understanding human variability in mating preferences is apt to involve more detailed knowledge of the links between these preferences and biological and chemical mechanisms associated with sexual motivation, sexual arousal, and sexual functioning.