Regulation of Treg Functions by the Ubiquitin Pathway

2021 ◽  
pp. 47-62
Author(s):  
Elena Montauti ◽  
Deyu Fang
Keyword(s):  
Ubiquitin Pathway

scholarly journals Investigation of energy metabolic dynamism in hyperthermia-resistant ovarian and uterine cancer cells under heat stress

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Taisei Kanamori ◽  
Natumi Miyazaki ◽  
Shigeki Aoki ◽  
Kousei Ito ◽  
Akihiro Hisaka ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Uterine Cancer ◽  
Mitochondrial Oxidative Phosphorylation ◽  
Ubiquitin Pathway ◽  
Atp Production ◽  
Skov3 Cells ◽  
Pathway Analyses ◽  
Rate Limiting ◽  
Glycolytic Rate ◽  
The Warburg Effect

AbstractDespite progress in the use of hyperthermia in clinical practice, the thermosensitivity of cancer cells is poorly understood. In a previous study, we found that sensitivity to hyperthermia varied between ovarian and uterine cancer cell lines. Upon hyperthermia, glycolytic enzymes decreased in hyperthermia-resistant SKOV3 cells. However, the mechanisms of glycolysis inhibition and their relationship with thermoresistance remain to be explored. In this study, metabolomic analysis indicated the downregulation of glycolytic metabolites in SKOV3 cells after hyperthermia. Proteomic and pathway analyses predicted that the ubiquitin pathway was explicitly activated in resistant SKOV3 cells, compared with hyperthermia-sensitive A2780 cells, and STUB1, a ubiquitin ligase, potentially targeted PKM, a glycolytic rate-limiting enzyme. PKM is degraded via ubiquitination upon hyperthermia. Although glycolysis is inactivated by hyperthermia, ATP production is maintained. We observed that oxygen consumption and mitochondrial membrane potential were activated in SKOV3 cells but suppressed in A2780 cells. The activation of mitochondria could compensate for the loss of ATP production due to the suppression of glycolysis by hyperthermia. Although the physiological significance has not yet been elucidated, our results demonstrated that metabolomic adaptation from the Warburg effect to mitochondrial oxidative phosphorylation could contribute to thermoresistance in ovarian and uterine cancer cells.

scholarly journals Circadian Regulation of mTOR by the Ubiquitin Pathway in Renal Cell Carcinoma

2013 ◽  
Vol 74 (2) ◽  
pp. 543-551 ◽  
Author(s):  
Hiroyuki Okazaki ◽  
Naoya Matsunaga ◽  
Takashi Fujioka ◽  
Fumiyasu Okazaki ◽  
Yui Akagawa ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Circadian Regulation ◽  
Ubiquitin Pathway

scholarly journals Ubiquitin pathway and ovarian cancer

2012 ◽  
Vol 19 (6) ◽  
Author(s):  
Z. Rao ◽  
Y. Ding
Keyword(s):  
Ovarian Cancer ◽  
Ubiquitin Pathway

DNA damage inhibits proteolysis of the B-type cyclin Clb5 in S. cerevisiae

1997 ◽  
Vol 110 (15) ◽  
pp. 1813-1820
Author(s):  
D. Germain ◽  
J. Hendley ◽  
B. Futcher
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Tyrosine Phosphorylation ◽  
Cell Cycle Progression ◽  
Cycle Phase ◽  
Cycle Progression ◽  
Ubiquitin Pathway ◽  
Cyclin Dependent Kinases ◽  
Transition Points ◽  
Checkpoint Genes

Cell cycle progression is mediated by waves of specific cyclin dependent kinases (CDKs) in all eukaryotes. Cyclins are degraded by the ubiquitin pathway of proteolysis. The recent identification of several components of the cyclin proteolysis machinery has highlighted both the importance of proteolysis at multiple transition points in the cell cycle and the involvement of other substrates degraded by the same machinery. In this study, we have investigated the effects of DNA damage on the cyclin proteolytic machinery in Saccharomyces cerevisiae. We find that the half-life of the B-type cyclin Clb5 is markedly increased following DNA damage while that of G1 cyclins is not. This effect is independent of cell cycle phase. Clb5 turnover requires p34CDC28 activity. Stabilisation of Clb5 correlates with an increase in tyrosine phosphorylation of p34CDC28, but stabilisation does not require this tyrosine phosphorylation. The stabilisation is independent of the checkpoint genes Mec1 and Rad53. These observations establish a new link between the regulation of proteolysis and DNA damage.

A novel, arsenite-sensitive E2 of the ubiquitin pathway: purification and properties

Biochemistry ◽  
1989 ◽  
Vol 28 (14) ◽  
pp. 6035-6041 ◽  
Author(s):  
Nancy S. Klemperer ◽  
Erica S. Berleth ◽  
Cecile M. Pickart
Keyword(s):  
Ubiquitin Pathway ◽  
Purification And Properties
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