Factors influencing transient gene expression in bean (Phaseolus vulgaris L.) using an electrical particle acceleration device

1993 ◽  
Vol 12 (9) ◽  
pp. 483-490 ◽  
Author(s):  
F. J. L. Aragao ◽  
M. -F. Grossi de Sa ◽  
M. R. Davey ◽  
A. C. M. Brasileiro ◽  
J. C. Faria ◽  
...  
Keyword(s):  
Gene Expression ◽  
Particle Acceleration ◽  
Phaseolus Vulgaris ◽  
Transient Gene Expression ◽  
Phaseolus Vulgaris L ◽  
Factors Influencing ◽  
Electrical Particle

scholarly journals Factors influencing efficiency of transient gene expression in the red macrophyte Porphyra yezoensis

Plant Science ◽  
2008 ◽  
Vol 174 (3) ◽  
pp. 329-339 ◽  
Author(s):  
Satoru Fukuda ◽  
Koji Mikami ◽  
Toshiki Uji ◽  
Eun-Jeong Park ◽  
Toshiharu Ohba ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Gene Expression ◽  
Porphyra Yezoensis ◽  
Factors Influencing

Variation in the enzyme activity and gene expression of myo-inositol-3-phosphate synthase and phytate accumulation during seed development in common bean (Phaseolus vulgaris L.)

2007 ◽  
Vol 29 (3) ◽  
pp. 265-271 ◽  
Author(s):  
Cileide Maria Medeiros Coelho ◽  
Vagner Augusto Benedito ◽  
Antonio Figueira ◽  
Victor Alexandre Vitorello ◽  
Ricardo Antunes Azevedo
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Seed Development ◽  
Phaseolus Vulgaris L ◽  
Phosphate Synthase

Factors influencing regeneration and Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris L.)

2012 ◽  
Vol 7 (1) ◽  
pp. 59-70 ◽  
Author(s):  
Gerardine Mukeshimana ◽  
Yumin Ma ◽  
Aaron E. Walworth ◽  
Guo-qing Song ◽  
James D. Kelly
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Phaseolus Vulgaris L ◽  
Factors Influencing

Biological nitrogen fixation and nifH gene expression in dry beans (Phaseolus vulgaris L.)

2014 ◽  
Vol 94 (2) ◽  
pp. 203-212 ◽  
Author(s):  
Zafrin Akter ◽  
Binod B. Pageni ◽  
Newton Z. Lupwayi ◽  
Parthiba M. Balasubramanian
Keyword(s):  
Gene Expression ◽  
Nitrogen Fixation ◽  
Phaseolus Vulgaris ◽  
Biological Nitrogen Fixation ◽  
Nifh Gene ◽  
Rapid Test ◽  
Dry Beans ◽  
Phaseolus Vulgaris L ◽  
Dry Bean ◽  
Biological Nitrogen

Akter, Z., Pageni, B. B., Lupwayi, N. Z. and Balasubramanian, P. M. 2014. Biological nitrogen fixation and nif H gene expression in dry beans ( Phaseolus vulgaris L.). Can. J. Plant Sci. 94: 203–212. Dry bean (Phaseolus vulgaris L.) is one of the least efficient nitrogen (N2) fixers among legumes (approximately 30 to 50 kg N ha−1). To identify efficient N2 fixers, 22 dry bean genotypes were screened for symbiotic N2-fixation in potted low-N soil in the greenhouse under four treatments: (1) uninoculated, (2) inoculated with commercial Rhizobium leguminosarum bv. phaseoli inoculant Nitrastik-D®, (3) inoculated with commercial Rhizobium inoculant Nodulator®, and (4) fertilizer N added. Expression of nitrogenase reductase (nifH) gene in selected uninoculated genotypes (nodulated by indigenous rhizobia) was quantified by reverse transcription polymerase chain reaction (RT-PCR) to know if expression of this gene was an indicator of N2-fixation. Nitrogen fixation potential varied among the genotypes and between the rhizobia inoculants. Genotypes differed in shoot (5.17 to 8.39 g plant−1) and root (1.71 to 6.44 g plant−1) dry weights, nodule number (0 to 155 nodules plant−1) and mass (0.055 to 0.250 mg plant−1), and N2-fixed (0 to 129 mg N plant−1). Genotypes GH–196, FR 266 and AC Redbond fixed the most N2 (60 to 112 mg N plant−1) with either rhizobial inoculant, and Viva fixed the highest amount (129 mg N plant−1) when inoculated with Nitrastik-D. In contrast, AC Black Diamond, Island, Winchester, AC Polaris, LEF 2RB and SEA 5 had low N2-fixing potential (3 to 23 mg N plant−1) in inoculated treatments. The qRT-PCR analysis indicated that nifH gene expression was 5 fold higher in GH-196 compared to the non-nodulating mutant R99, which further confirmed the high N2-fixation capacity of GH-196. After confirmation in the field, dry bean genotypes with improved N2-fixation potential identified in this study may be used as parents in breeding programs in the development of future cultivars. The nifH gene expression may be used as a rapid test to select dry beans with high N2-fixation potential.

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