YAC analysis and minimal tiling path construction for chromosome 21q

1995 ◽  
Vol 21 (6) ◽  
pp. 399-414 ◽  
Author(s):  
Katheleen Gardiner ◽  
Sharon Graw ◽  
Hitoshi Ichikawa ◽  
Misao Ohki ◽  
Anthony Joetham ◽  
...  

BioTechniques ◽  
2004 ◽  
Vol 37 (2) ◽  
pp. 282-284 ◽  
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Volker Hollich ◽  
Eric Johnson ◽  
Eileen E. Furlong ◽  
Boris Beckmann ◽  
Joseph Carlson ◽  
...  


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pp. 2152-2163 ◽  
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F. W. Engler


2000 ◽  
Vol 5 (4) ◽  
pp. 189-203 ◽  
Author(s):  
BIXING HUANG ◽  
CYNTHIA B. WHITCHURCH ◽  
LARRY CROFT ◽  
SCOTT A. BEATSON ◽  
JOHN S. MATTICK


2000 ◽  
pp. 169-197
Author(s):  
Jingling Xue
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2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Heiner Kuhl ◽  
Elena Sarropoulou ◽  
Mbaye Tine ◽  
Georgios Kotoulas ◽  
Antonios Magoulas ◽  
...  

This study presents the first comparative BAC map of the gilthead sea bream (Sparus aurata), a highly valuated marine aquaculture fish species in the Mediterranean. High-throughput end sequencing of a BAC library yielded 92,468 reads (60.6 Mbp). Comparative mapping was achieved by anchoring BAC end sequences to the three-spined stickleback (Gasterosteus aculeatus) genome. BACs that were consistently ordered along the stickleback chromosomes accounted for 14,265 clones. A fraction of 5,249 BACs constituted a minimal tiling path that covers 73.5% of the stickleback chromosomes and 70.2% of the genes that have been annotated. The N50 size of 1,485 “BACtigs” consisting of redundant BACs is 337,253 bp. The largest BACtig covers 2.15 Mbp in the stickleback genome. According to the insert size distribution of mapped BACs the sea bream genome is 1.71-fold larger than the stickleback genome. These results represent a valuable tool to researchers in the field and may support future projects to elucidate the whole sea bream genome.



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