Identification of AFLP markers linked to resistance of cowpea (Vigna unguiculata L.) to parasitism by Striga gesnerioides

2001 ◽  
Vol 102 (6-7) ◽  
pp. 1029-1036 ◽  
Author(s):  
J. T. Ouédraogo ◽  
V. Maheshwari ◽  
D. K. Berner ◽  
C.-A. St-Pierre ◽  
F. Belzile ◽  
...  
Keyword(s):  
Vigna Unguiculata ◽  
Aflp Markers ◽  
Striga Gesnerioides

AFLP markers linked to resistance against Striga gesnerioides race 1 in cowpea (Vigna unguiculata)

Genome ◽  
2002 ◽  
Vol 45 (5) ◽  
pp. 787-793 ◽  
Author(s):  
Jeremy T Ouédraogo ◽  
Jean-Baptiste Tignegre ◽  
Michael P Timko ◽  
François J Belzile
Keyword(s):  
Molecular Markers ◽  
Vigna Unguiculata ◽  
Fragment Length Polymorphism ◽  
Bulked Segregant Analysis ◽  
Aflp Markers ◽  
Resistant Cultivar ◽  
Aflp Analysis ◽  
Striga Gesnerioides ◽  
Striga Resistance ◽  
Race 1

Amplified fragment length polymorphism (AFLP) analysis was used in combination with bulked segregant analysis (BSA) to identify molecular markers linked to two cowpea (Vigna unguiculata (L.) Walp.) genes conferring resistance to Striga gesnerioides race 1. After AFLP analysis of an F2 population derived from a cross between the resistant cultivar Gorom and the susceptible cultivar Tvx 3236, seven AFLP markers were identified that are linked to Rsg3, the gene conferring race 1 resistance in 'Gorom'. The distances between these markers and Rsg3 ranged from 9.9 to 2.5 cM, with two markers, E-AGA/M-CTA460 and E-AGA/M-CAG300, flanking Rsg3 at 2.5 and 2.6 cM, respectively. Analysis of a second F2 population derived from the cross between 'Tvx 3236' and the resistant cultivar IT81D-994 identified five AFLP markers linked to the race 1 resistance gene 994-Rsg present in 'IT81D-994'. The two markers showing the tightest linkage to the994-Rsg locus were E-AAG/M-AAC450 and E-AAG/M-AAC150 at 2.1 and 2.0 cM, respectively. Two of the markers linked to 994-Rsg, E-AGA/M-CAG300 and E-AGA/M-CAG450, were also linked to Rsg3. The identification of molecular markers in common between the two sources of race 1 resistance suggests that either Striga resistance genes are clustered in these plants or that these loci are allelic. Mapping of the resistance loci within the cowpea genome revealed that three markers linked to Rsg3 and (or) 994-Rsg are located on linkage group 6.Key words: cowpea, AFLP markers, Striga resistance, bulked segregant analysis.

Genetic diversity of cowpea (Vigna unguiculata L. Walp) cultivars in Burkina Faso resistant to Striga gesnerioides

2010 ◽  
Vol 9 (48) ◽  
pp. 8146-8153 ◽  
Author(s):  
Sawadogo Mahamadou ◽  
T Ouedraogo Jeremy ◽  
S Gowda Bhavani ◽  
P Timko Michael
Keyword(s):  
Genetic Diversity ◽  
Burkina Faso ◽  
Vigna Unguiculata ◽  
Striga Gesnerioides

scholarly journals The genome of cowpea (Vigna unguiculata [L.] Walp.)

2019 ◽  
Author(s):  
Stefano Lonardi ◽  
María Muñoz-Amatriaín ◽  
Qihua Liang ◽  
Shengqiang Shu ◽  
Steve I. Wanamaker ◽  
...  
Keyword(s):  
Vigna Unguiculata ◽  
Single Molecule ◽  
Warm Season ◽  
Multiple Organ ◽  
Sub Saharan Africa ◽  
Parasitic Weed ◽  
Nutritional Security ◽  
Striga Gesnerioides ◽  
Sub Saharan ◽  
Food And Nutritional Security

ABSTRACTCowpea (Vigna unguiculata [L.] Walp.) is a major crop for worldwide food and nutritional security, especially in sub-Saharan Africa, that is resilient to hot and drought-prone environments. A high-quality assembly of the single-haplotype inbred genome of cowpea IT97K-499-35 was developed by exploiting the synergies between single molecule real-time sequencing, optical and genetic mapping, and a novel assembly reconciliation algorithm. A total of 519 Mb is included in the assembled sequences. Nearly half of the assembled sequence is composed of repetitive elements, which are enriched within recombination-poor pericentromeric regions. A comparative analysis of these elements suggests that genome size differences between Vigna species are mainly attributable to changes in the amount of Gypsy retrotransposons. Conversely, genes are more abundant in more distal, high-recombination regions of the chromosomes; there appears to be more duplication of genes within the NBS-LRR and the SAUR-like auxin superfamilies compared to other warm-season legumes that have been sequenced. A surprising outcome of this study is the identification of a chromosomal inversion of 4.2 Mb among landraces and cultivars, which includes a gene that has been associated in other plants with interactions with the parasitic weed Striga gesnerioides. The genome sequence also facilitated the identification of a putative syntelog for multiple organ gigantism in legumes. A new numbering system has been adopted for cowpea chromosomes based on synteny with common bean (Phaseolus vulgaris).

Virulence characteristics of a new race of the parasitic angiosperm, Striga gesnerioides, from southern Benin on cowpea (Vigna unguiculata)

Euphytica ◽  
1994 ◽  
Vol 72 (3) ◽  
pp. 183-188 ◽  
Author(s):  
J. A. Lane ◽  
T. H. M. Moore ◽  
D. V. Child ◽  
K. F. Cardwell ◽  
B. B. Singh ◽  
...  
Keyword(s):  
Vigna Unguiculata ◽  
Striga Gesnerioides ◽  
Parasitic Angiosperm ◽  
New Race ◽  
Southern Benin

Marker assisted selection for resistance to Striga gesnerioides in Cowpea (Vigna unguiculata L. Walp)

2017 ◽  
Vol 2 (5) ◽  
pp. 97-103 ◽  
Author(s):  
Victoria Larweh ◽  
◽  
Richard Akromah ◽  
Stephen Amoah ◽  
James Y. Asibuo ◽  
...  
Keyword(s):  
Vigna Unguiculata ◽  
Marker Assisted Selection ◽  
Striga Gesnerioides ◽  
Selection For
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