Development of a sensitive sandwich enzyme-linked immunosorbent assay test kit for reliable detection of peanut residues in processed food

Author(s):  
Yu Ji ◽  
Hong Lin ◽  
Jinlong Zhao ◽  
Jiukai Zhang ◽  
Hongbing Liu ◽  
...  
2011 ◽  
Vol 94 (5) ◽  
pp. 1519-1530
Author(s):  
Arthur Trombley ◽  
Titan Fan ◽  
Robert LaBudde

Abstract The level of total aflatoxin contamination was analyzed in naturally contaminated and spiked samples of corn and peanut using the Aflatoxin Plate Kit. This kit is an enzyme-linked immunosorbent assay (ELISA) suitable for rapid testing of grains and peanuts. The assay was evaluated for ruggedness and linearity of the standard curve. The test kit results were then statistically evaluated for accuracy, precision, and correlation to a validated HPLC method (AOAC 994.08). The results were verified by an independent laboratory.


2011 ◽  
Vol 26 (6) ◽  
pp. 695-699 ◽  
Author(s):  
Charalampos Agakidis ◽  
Thomais Karagiozoglou-Lampoudi ◽  
Marina Kalaitsidou ◽  
Theodoros Papadopoulos ◽  
Afroditi Savvidou ◽  
...  

2006 ◽  
Vol 89 (6) ◽  
pp. 1600-1608 ◽  
Author(s):  
Rieko Matsuda ◽  
Yasuo Yoshioka ◽  
Hiroshi Akiyama ◽  
Kenichi Aburatani ◽  
Yumiko Watanabe ◽  
...  

Abstract The labeling of 5 major allergenic ingredients (egg, milk, wheat, buckwheat, and peanut) is mandatory in Japan, and 2 series of enzyme-linked immunosorbent assay (ELISA) kits have been established as official screening methods. However, these official methods have not provided the necessary sensitivity, due in part to poor extraction efficiency. To address this need, 2 novel ELISA kits have been developed: the FASTKIT ELISA Ver. II Series and the FASPEK Allergenic Substances Detection Kit. The new kit systems use an improved extraction buffer that can extract insoluble proteins produced by processing and feature new antibodies that bind to the denatured proteins extracted with the new extraction buffer. The analytical performances of the 2 new ELISA kit series were evaluated in an interlaboratory study. Ten laboratories participated in the study and determined the major allergenic ingredients contained in 5 types of model processed food. The 2 ELISAs displayed fairly good reproducibility and sufficient recovery.


1986 ◽  
Vol 49 (7) ◽  
pp. 523-525 ◽  
Author(s):  
STANLEY M. HARMON ◽  
DONALD A. KAUTTER

A reversed passive latex agglutination (RPLA) test kit for Clostridium perfringens enterotoxin (CPE) marketed by the Denka-Seiken Co., Tokyo, Japan, was evaluated by using culture supernatant fluids and extracts from feces of food poisoning patients. Nanograms of CPE were detectable with the assay and the reaction was specific, as shown by parallel activity in a double antibody enzyme-linked immunosorbent assay (ELISA). Although less sensitive, the RPLA method is easier to perform than the ELISA and counterimmunoelectrophoresis, both of which require special test reagents and equipment not generally available.


2020 ◽  
Vol 65 (11) ◽  
pp. 683-687
Author(s):  
S. G. Mardanly ◽  
A. S. Avdonina ◽  
S. G. Mamedova

A new original Russian test kit for the detection of IgG-antibodies to the causative agent of COVID-19 - coronavirus SARS-CoV-2 by the method of enzyme-linked immunosorbent assay (ELISA) on a solid-phase «ELISA-SARS-CoV-2-AT-G» has been developed. In comparative tests with similar test systems «Vitrotest® SARS-CoV-2 IgG» (Vitrotest, Ukraine) and «Anti-SARS-Cov-2 ELISA (IgG)» (EUROIMMUN AG, Germany) high diagnostic efficiency of the new test system was shown.


2008 ◽  
Vol 71 (3) ◽  
pp. 590-594 ◽  
Author(s):  
ERIC A. E. GARBER

Recent cases of adulteration with melamine have led to the need for rapid and reliable screening methods. To meet this need, commercial enzyme-linked immunosorbent assay (ELISA) test kits for the detection of triazines were evaluated. The recently released Melamine Plate kit (Abraxis, Warminster, Pa.) displayed a limit of detection of 9 ng/ml for melamine in phosphate-buffered saline (PBS) and approximately 1 μg/ml for melamine added to dog food. An atrazine ELISA test kit produced by Abraxis required 0.2 mg/ml to generate a response more than four times the standard deviation from background. In contrast, with the EnviroGard Triazine Plate kit (Strategic Diagnostics, Inc., Newark, Del.), 1.5 mg/ml melamine in PBS generated a signal only one standard deviation from background, which was insufficient to define a limit of detection. Extraction based on dilution with 105 mM sodium phosphate/75 mM NaCl/2.5% nonfat milk/0.05% Tween 20 (UD) enabled detection of fivefold less melamine in dog food than did use of the procedure recommended by the manufacturer, which entailed extraction into 60% methanol, sonication, centrifugation, filtration, and further dilution into 10% methanol/PBS. Using the Abraxis Melamine ELISA, both extraction protocols yielded identical results with a dog food sample adulterated with mela-mine. The recovery of melamine spiked into gravy from dog food using UD was 74% ± 4%. In conclusion, the recently released Abraxis ELISA for melamine proved to be a useful alternative to more cumbersome methods.


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