Development and application of a heterologous internal standard for polymerase-chain-reaction-based detection of Campylobacter jejuni and Campylobacter coli in foods

1999 ◽  
Vol 209 (3-4) ◽  
pp. 180-184 ◽  
Author(s):  
J. A. Straub ◽  
C. Hertel ◽  
D. Mäde ◽  
W. P. Hammes
Keyword(s):  
Polymerase Chain Reaction ◽  
Campylobacter Jejuni ◽  
Internal Standard ◽  
Campylobacter Coli ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Survey of chicken abattoir for the presence of Campylobacter jejuni and Campylobacter coli

2006 ◽  
Vol 48 (6) ◽  
pp. 307-310 ◽  
Author(s):  
Ana L.L. Cortez ◽  
Angela C.F.B. Carvalho ◽  
Eliana Scarcelli ◽  
Simone Miyashiro ◽  
Ana M.C. Vidal-Martins ◽  
...  
Keyword(s):  
Public Health ◽  
Polymerase Chain Reaction ◽  
Campylobacter Jejuni ◽  
Intestinal Tract ◽  
Campylobacter Coli ◽  
Improve Quality ◽  
Chain Reaction ◽  
Paulo State ◽  
Polymerase Chain ◽  
Campylobacter Spp

The genus Campylobacter is of great importance to public health because it includes several species that may cause diarrhea. These species may be found in water, food and in the intestinal tract of chickens. This study investigated the presence of Campylobacter jejuni and Campylobacter coli in chicken abattoirs in São Paulo State, Brazil. A total of 288 samples of feces, feathers, scald water, evisceration water, chiller water, and the rinse water of eviscerated, not eviscerated and chilled carcasses were collected in six chicken abattoirs. Polymerase Chain Reaction (PCR) was performed in Campylobacter spp.-positive isolates using the gene HIP, specific for hippuricase enzyme from Campylobacter jejuni and aspartokinase gene, specific to detect Campylobacter coli. The percentage of positive isolates of Campylobacter jejuni was 4.9% (14/288). Isolation was greater in feces samples (22%, 8/36). One sample was positive for the species C. coli. In conclusion, the results indicate that it is necessary to improve quality control for Campylobacter spp. in chicken abattoirs.

Detection of Campylobacter jejuni and Campylobacter coli in Foods by Enrichment Culture and Polymerase Chain Reaction Enzyme-Linked Immunosorbent Assay

2002 ◽  
Vol 65 (5) ◽  
pp. 760-767 ◽  
Author(s):  
F. J. BOLTON ◽  
A. D. SAILS ◽  
A. J. FOX ◽  
D. R. A. WAREING ◽  
D. L. A. GREENWAY
Keyword(s):  
Polymerase Chain Reaction ◽  
Campylobacter Jejuni ◽  
Predictive Value ◽  
Enrichment Culture ◽  
Food Samples ◽  
Campylobacter Coli ◽  
Chain Reaction ◽  
Enrichment Cultures ◽  
Selective Agar ◽  
Polymerase Chain

A polymerase chain reaction (PCR) assay based on a solution hybridization format with colorimetric end-point detection (PCR ELISA) was investigated for the specific detection of Campylobacter jejuni and Campylobacter coli in food samples following enrichment culture. One hundred fifteen samples of raw meat and offal (poultry, porcine, ovine, and bovine), raw shellfish, and artificially contaminated milk were enriched in blood-free Campylobacter Enrichment Broth for 48 h. Enrichment cultures were subcultured to Campylobacter blood-free selective agar plates, and presumptive isolates were identified by phenotypic methods. DNA was extracted from 1-ml aliquots of the enrichment cultures using a rapid extraction method, and the DNA was used as the template in a PCR ELISA. A comparison of the PCR ELISA with the enrichment culture and subculture to selective agar method showed that the results of 112 of the 115 samples tested were in agreement by both methods. Seventy-one of the various food samples were positive in the PCR ELISA, and 70 samples were positive by culture. The PCR ELISA had a sensitivity of 99% and a specificity of 96%, with a positive predictive value of 97% and a negative predictive value of 98%. The PCR ELISA is a rapid, sensitive, and specific method for the detection of C. jejuni and C. coli in foods following enrichment culture and significantly reduces the time required for their detection.

scholarly journals Identification of campylobacter species isolates with phenotypic methods and polymerase chain reaction

2014 ◽  
Vol 142 (11-12) ◽  
pp. 708-712 ◽  
Author(s):  
Biljana Miljkovic-Selimovic ◽  
Tatjana Babic ◽  
Branislava Kocic ◽  
Aleksandra Matkic ◽  
Ljiljana Ristic
Keyword(s):  
Polymerase Chain Reaction ◽  
Campylobacter Jejuni ◽  
Species Level ◽  
Campylobacter Coli ◽  
Chain Reaction ◽  
Biochemical Tests ◽  
Polymerase Chain ◽  
Campylobacter Species ◽  
Phenotypic Methods

Introduction. Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) are the most common bacterial causes of enterocolitis in humans. However, identification of the species level is not always possible using standard biochemical tests. Objective. Therefore, the goal of this study was to identify these microorganisms by both phenotyping and polymerase chain reaction (PCR) technique. Methods. A total of 153 species of thermophilic campylobacters were examined with standard biochemical tests and PCR technique to prove hipO genes of C. jejuni and asp genes of C. coli. Results Standard biochemical tests enabled the speciation of 121 strains of C. jejuni, while application of PCR detected 126 C. jejuni strains. Conclusion. PCR technique allowed not only identification of hippurate-positive C. jejuni, but also hipuratnegative strains of C. jejuni which otherwise would be detected as C. coli if only biochemical tests were applied.

Specific detection of Campylobacter jejuni and Campylobacter coli by using polymerase chain reaction.

1992 ◽  
Vol 30 (10) ◽  
pp. 2613-2619 ◽  
Author(s):  
B A Oyofo ◽  
S A Thornton ◽  
D H Burr ◽  
T J Trust ◽  
O R Pavlovskis ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Campylobacter Jejuni ◽  
Campylobacter Coli ◽  
Specific Detection ◽  
Chain Reaction ◽  
Polymerase Chain
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