scholarly journals Generation and functional analysis of single chain variable fragments (scFvs) targeting the nucleocapsid protein of Porcine epidemic diarrhea virus

2022 ◽  
Author(s):  
Fengqing Wang ◽  
Man Wang ◽  
Lei Zhang ◽  
Manling Cheng ◽  
Mei Li ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Porcine Epidemic Diarrhea Virus ◽  
Nucleocapsid Protein ◽  
Single Chain ◽  
Diarrhea Virus ◽  
Single Chain Variable Fragments ◽  
Porcine Epidemic Diarrhea

scholarly journals Molecular Characterizations of Subcellular Localization Signals in the Nucleocapsid Protein of Porcine Epidemic Diarrhea Virus

Viruses ◽  
2014 ◽  
Vol 6 (3) ◽  
pp. 1253-1273 ◽  
Author(s):  
Da Shi ◽  
Maojie Lv ◽  
Jianfei Chen ◽  
Hongyan Shi ◽  
Sha Zhang ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Porcine Epidemic Diarrhea Virus ◽  
Nucleocapsid Protein ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals BST2 suppresses porcine epidemic diarrhea virus replication by targeting and degrading virus nucleocapsid protein with selective autophagy

Autophagy ◽  
2019 ◽  
Vol 16 (10) ◽  
pp. 1737-1752 ◽  
Author(s):  
Ning Kong ◽  
Tongling Shan ◽  
Hua Wang ◽  
Yajuan Jiao ◽  
Yewen Zuo ◽  
...  
Keyword(s):  
Virus Replication ◽  
Porcine Epidemic Diarrhea Virus ◽  
Nucleocapsid Protein ◽  
Diarrhea Virus ◽  
Selective Autophagy ◽  
Porcine Epidemic Diarrhea

scholarly journals Cellular hnRNP A1 Interacts with Nucleocapsid Protein of Porcine Epidemic Diarrhea Virus and Impairs Viral Replication

Viruses ◽  
2018 ◽  
Vol 10 (3) ◽  
pp. 127 ◽  
Author(s):  
Zhonghua Li ◽  
Wei Zeng ◽  
Shiyi Ye ◽  
Jian Lv ◽  
Axiu Nie ◽  
...  
Keyword(s):  
Viral Replication ◽  
Porcine Epidemic Diarrhea Virus ◽  
Nucleocapsid Protein ◽  
Hnrnp A1 ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

Escherichia coli expressing single-chain Fv on the cell surface as a potential prophylactic of porcine epidemic diarrhea virus

Vaccine ◽  
2009 ◽  
Vol 27 (14) ◽  
pp. 2030-2036 ◽  
Author(s):  
Hyun-Mi Pyo ◽  
In-Joong Kim ◽  
Seong-Hee Kim ◽  
Hyun-Soo Kim ◽  
Soo-Dong Cho ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Porcine Epidemic Diarrhea Virus ◽  
Single Chain ◽  
Diarrhea Virus ◽  
Single Chain Fv ◽  
Porcine Epidemic Diarrhea

scholarly journals Generation and Characterization of a Spike Glycoprotein Domain A-Specific Neutralizing Single-Chain Variable Fragment against Porcine Epidemic Diarrhea Virus

Vaccines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 833
Author(s):  
Chia-Yu Chang ◽  
Yong-Sheng Wang ◽  
Jou-Fei Wu ◽  
Tzu-Jing Yang ◽  
Yen-Chen Chang ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Expression System ◽  
Single Chain ◽  
Variable Regions ◽  
Diarrhea Virus ◽  
Single Chain Fragment Variable ◽  
Neutralizing Monoclonal Antibody ◽  
Porcine Epidemic Diarrhea ◽  
Lactogenic Immunity

The emergence of the genotype (G) 2 and re-emergence of the G1 porcine epidemic diarrhea virus (PEDV) has caused severe economic impacts in the past decade. Developments of efficient vaccines against new variants of PEDV have been challenging, not least because of the difficulties in eliciting mucosal and lactogenic immunity. A single-chain fragment variable (scFv) capable of efficient antigen recognition is an alternative to vaccination and treatment of a viral infection. In the present study, the variable regions of the light chain and the heavy chain of a G2b PEDV spike domain A (S1A)-specific neutralizing monoclonal antibody (mAb) were sequenced, constructed with a (G4S) x3 linker, and produced by a mammalian protein expression system. Our results demonstrated that the PEDV S1A domain scFv was able to bind to S proteins of both G1 and G2b PEDVs. Nevertheless, the scFv was only capable of neutralizing the homologous G2b PEDV but not the G1 PEDV. The binding ability of the G2b-specific neutralizing scFv was not able to predict the neutralizing ability toward heterologous PEDV. The anti-PEDV S1A scFv presented herein serves as a potential therapeutic candidate against the virulent G2b PEDV.

scholarly journals Single Chain Fragment Variable (scFv) Antibodies Targeting the Spike Protein of Porcine Epidemic Diarrhea Virus Provide Protection against Viral Infection in Piglets

Viruses ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 58 ◽  
Author(s):  
Fanqing Zhang ◽  
Yuxue Chen ◽  
Yong Ke ◽  
Lei Zhang ◽  
Bo Zhang ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Passive Immunization ◽  
Spike Protein ◽  
Single Chain ◽  
Effective Prevention ◽  
Chain Fragment ◽  
Diarrhea Virus ◽  
Single Chain Fragment Variable ◽  
Porcine Epidemic Diarrhea ◽  
Single Chain Fragment

Porcine epidemic diarrhea virus (PEDV) is a highly contagious coronavirus that causes severe diarrhea and death in neonatal piglets. Passive immunization with neutralizing antibodies against PEDV is an effective prevention measure. In this study, single chain fragment variable (scFv) antibodies against PEDV were screened from the porcine scFv phage display library. After four rounds of biopanning, scFvs that showed higher affinity to the PEDV antigen were selected for further study. The scFv genes were cloned into the expression plasmid for recombinant protein expression. These scFvs were shown to inhibit PEDV infectivity by the plaque reduction neutralization assay. Immunofluorescence assay (IFA) revealed that the epitopes recognized by these scFvs were in the S1 region of the spike protein. The potential of scFvs to provide prevention against PEDV infections in piglets was further investigated. Piglets orally administered scFvs showed no to mild clinical symptoms, significantly less viral shedding, no mortality and no intestinal lesions. The field application also revealed that the survival rate of piglets was significantly increased by oral administration of scFvs. Our data support the potential role of scFvs in the prevention and treatment of PEDV infection.

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