Treatment of Synthetic Ammonium Sulfate Wastewater by Mixed Culture of Chlorella pyrenoidosa and Enriched Nitrobacteria

Author(s):  
Lei Qin ◽  
Siran Feng ◽  
Pinzhong Feng ◽  
Zhongming Wang ◽  
Shunni Zhu
2001 ◽  
Vol 7 (3) ◽  
pp. 177-182 ◽  
Author(s):  
Deniz Tanyolaç ◽  
Bekir Salih ◽  
Abdurrahman Tanyolaç

2020 ◽  
Vol 13 (1) ◽  
pp. 138
Author(s):  
Fatin Natasha Amira Muliadi ◽  
Mohd Izuan Effendi Halmi ◽  
Samsuri Bin Abdul Wahid ◽  
Siti Salwa Abd Gani ◽  
Uswatun Hasanah Zaidan ◽  
...  

In the present study, a mixed culture from a local agricultural soil sample was isolated for Metanil Yellow (MY) dye decolorization. The metagenomic analysis confirmed that 42.6% has been dominated by genus Bacillus, while Acinetobacter (14.0%) is present in the microbial communities of the mixed culture. For fungi diversity analysis, around 97.0% was “unclassified” fungi and 3% was Candida. The preliminary investigation in minimal salt media (MSM) showed that 100% decolorization was achieved after 24 h of incubation. Response surface methodology (RSM) was successfully applied using Box-Behnken design (BBD) to study the effect of four independent parameters—MY dye concentration, glucose concentration, ammonium sulfate concentration, and pH—on MY dye decolorization by the mixed bacterial culture. The optimal conditions predicted by the desirability function were 73 mg/L of MY, 1.934% glucose, 0.433 g/L of ammonium sulfate, and a pH of 7.097, with 97.551% decolorization The correlation coefficients (R2 and R2 adj) of 0.913 and 0.825 indicate that the established model is suitable to predict the effectiveness of dye decolorization under the investigated condition. The MY decolorization of the mixed bacterial culture was not affected by the addition of heavy metals in the growth media. Among the 10 heavy metals tested, only copper gave 56.19% MY decolorization, whereas the others gave almost 100% decolorization. The decolorization potential of the mixed bacterial culture indicates that it could be effective for future bioremediation of soil-contaminated sites and treatment solutions of water bodies polluted with the MY dye.


2019 ◽  
Vol 20 (12) ◽  
Author(s):  
Dia Septiani ◽  
HERMAN SURYADI ◽  
Abdul Mun'im ◽  
WIBOWO MANGUNWARDOYO

Abstract. Septiani D, Suryadi H, Mun’im A, Mangunwardoyo W. 2019. Production of cellulase from Aspergillus niger and Trichoderma reesei mixed culture in carboxymethylcellulose medium as sole carbon. Biodiversitas 20: 3539-3544. Cellulase is one of hydrolytic enzymes that breakdown cellulose into glucose. Cellulases are promising to be applied in natural products which may improve the yield of bioactive in plant extract through cellulose depolymerization. Cellulases from mixed culture of Aspergillus niger and Trichoderma reesei can produce a high cellulase activity because of the synergism activity among endoglucanase, exoglucanase, and also β-glucoside. Cellulase production and partial purification of monoculture and mixed culture (1:1) of these fungi on carboxymethylcellulose media were investigated in this study. Total cellulase activity was measured by filter paper assay followed by protein estimation with Bradford method. The crude extract of Aspergillus niger monoculture has the highest cellulase activity (0.131 U/mL, P<005) followed by mixed culture (0.109 U/mL) and Trichoderma reesei (0.106 U/mL). The cellulase activity of partially purified cellulase from mixed culture significantly increased (0.335, 0.348, 0.374 U/mL, P<0.05) compared to crude extract along with stepwise addition of ammonium sulfate. Cellulase activity of mixed culture at 80% ammonium sulfate increase up to 2.238-fold and showed highest value (P<0.05) compared to monocultures. In conclusion, combination of Aspergillus niger and Trichoderma reesei fungi in carboxymethyl cellulose media followed by 80% ammonium sulfate precipitation can be a promising cellulase production with high cellulase activity.


1968 ◽  
Vol 20 (03/04) ◽  
pp. 457-464 ◽  
Author(s):  
L Gonyea ◽  
R Herdman ◽  
R. A Bridges

SummaryAn anticoagulant occurring in 4 of 6 patients with SLE has been demonstrated by a sensitive assay utilizing an ammonium sulfate fraction of serum. The anticoagulant functions as an inhibitor of the activation of prothrombin. No species specificity was demonstrable. The inhibitor behaves clinically and chromatographically as an immunoglobulin, although an attempt to demonstrate directly the antibody nature of the inhibitor was not successful.A severe, apparently independent, decrease in the level of prothrombin was observed in the patient with hemorrhagic symptoms. In contrast to the anticoagulant activity, the low prothrombin has persisted during treatment.


2013 ◽  
Vol 10 (2) ◽  
pp. 29
Author(s):  
Normah Ismail ◽  
Nur' Ain Mohamad Kharoe

Unripe and ripe bilimbi (Averrhoa bilimbi L.) were ground and the extracted juices were partially purified by ammonium sulfate precipitation at the concentrations of 40 and 60% (w/v). The collected proteases were analysed for pH, temperature stability, storage stability, molecular weight distribution, protein concentration and protein content. Protein content of bilimbi fruit was 0.89 g. Protease activity of both the unripe and ripe fruit were optimum at pH 4 and 40°C when the juice were purified at 40 and 60% ammonium sulfate precipitation. A decreased in protease activity was observed during the seven days of storage at 4°C. Molecular weight distribution indicated that the proteases protein bands fall between IO to 220 kDa. Protein bands were observed at 25, 50 and 160 kDa in both the unripe and ripe bilimbi proteases purified with 40% ammonium sulfate, however, the bands were more intense in those from unripe bilimbi. No protein bands were seen in proteases purified with 60% ammonium sulfate. Protein concentration was higher for proteases extracted with 40% ammonium sulfate at both ripening stages. Thus, purification using 40% ammonium sulfate precipitation could be a successful method to partially purify proteases from bilimbi especially from the unripe stage. 


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