Expression and clinical role of small glutamine-rich tetratricopeptide repeat (TPR)-containing protein alpha (SGTA) as a novel cell cycle protein in NSCLC

CD147 is expressed on the cell surface of most tumor cells, which results in cancer cells proliferation, invasion, metastasis and angiogenes. Our previous study indicated that CD147 could promote invasion andmetastasis of prostate cancer. However the role of CD147 on cell proliferation has not to be explored inprostate cancer. In this study, the effects of CD147 on cell proliferation of hormone-independent prostatecancer (LNCaP-AI) was investigated. In the present study, cell cycle distribution was investigated by flowcytometry and cell cycle protein were analysis by wester blot. The results demonstrated that knock-donwn CD147 expression induced G0/G1 phase arrest, and expression of cyclin D1 has potential suppressed with western blot analysis. The results suggest that CD147 could inhibit cell prolifearion and as potential therapeutic application in treatment of proste cancer.

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An updated review of the pre-clinical role of microRNAs and their contribution to colorectal cancer

Current Molecular Medicine ◽

10.2174/1566524021666211213122619 ◽

2021 ◽

Vol 21 ◽

Author(s):

Narges Dastmalchi ◽

Reza Safaralizadeh ◽

Shahram Teimourian

Keyword(s):

Colorectal Cancer ◽

Cell Cycle ◽

Molecular Targets ◽

Biological Pathways ◽

Biological Processes ◽

Therapeutic Approaches ◽

Clinical Role ◽

Related Mortality

: Colorectal cancer (CRC) is one of the main causes of malignancy-related mortality worldwide. It was well-identified that microRNAs (miRNAs) decisively participate in cellular biological pathways; in a way that their deregulated expression causes CRC progression. miRNAs can control the translation and degradation of mRNAs by binding to various molecular targets involved in different biological processes, including growth, apoptosis, cell cycle, autophagy, angiogenesis, metastasis, etc. The functions of these dysregulated miRNAs may be either oncogenic or tumor-suppressive. Therefore, these miRNAs can be contributed to prognostic, diagnostic, and therapeutic approaches in CRC. In this study, we reviewed the tumor-suppressive and oncogenic functions of miRNAs in CRC and assessed their molecular activities in CRC development. However, further investigation for the involvement of dysregulated miRNAs in CRC progression is required.

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LINE-1 and the cell cycle: protein localization and functional dynamics

10.1101/147587 ◽

2017 ◽

Cited By ~ 1

Author(s):

Paolo Mita ◽

Aleksandra Wudzinska ◽

Xiaoji Sun ◽

Joshua Andrade ◽

Shruti Nayak ◽

...

Keyword(s):

Cell Cycle ◽

Human Genome ◽

Cell Biology ◽

Protein Localization ◽

Clear Understanding ◽

Cell Cycle Protein ◽

Functional Dynamics ◽

Human Genomic ◽

The Many

AbstractLINE-1/L1 retrotransposon sequences comprise 17% of the human genome. Among the many classes of mobile genetic elements, L1 is the only autonomous retrotransposon that still drives human genomic plasticity today. Through its co-evolution with the human genome, L1 has intertwined itself with host cell biology to aid its proliferation. However, a clear understanding of L1’s lifecycle and the processes involved in restricting its insertion and its intragenomic spreading remains elusive. Here we identify modes of L1 proteins’ entrance into the nucleus, a necessary step for L1 proliferation. Using functional, biochemical, and imaging approaches, we also show a clear cell cycle bias for L1 retrotransposition that peaks during the S phase. Our observations provide a basis for novel interpretations about the nature of nuclear and cytoplasmic L1 ribonucleoproteins (RNPs) and the potential role of DNA replication in L1 retrotransposition.

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320. THE ROLE OF FIZZY RELATED 1 IN MALE MEIOSIS

Reproduction Fertility and Development ◽

10.1071/srb10abs320 ◽

2010 ◽

Vol 22 (9) ◽

pp. 120

Author(s):

L. Hopkins ◽

V. Pye ◽

B. Fraser ◽

J. Holt ◽

K. Jones ◽

...

Keyword(s):

Cell Cycle ◽

Mammalian Cells ◽

Binding Capacity ◽

Cyclin B1 ◽

Male Meiosis ◽

Conditional Knockout ◽

Wild Type ◽

Cell Cycle Protein ◽

Sperm Analysis

Accurate chromosome segregation during mitosis and meiosis is facilitated by a regulatory complex known as the Anaphase Promoting Cyclosome (APC), an ubiquitin ligase complex that tags proteins with ubiquitin. Subsequently targeted proteins are recognised by the 26S proteosome and degraded. In mammalian cells, two temporally regulated co-activators are required for the APC to function; fizzy and fzr1. In studies of female oocyte development fzr1 has been demonstrated to play an important role in maintaining G2 arrest during meiosis by controlling spatial levels of the cell cycle protein Cyclin B1 but the role of Fzr1 in spermatogenesis remains unknown. Germ cell specific conditional knockout fzr1mice were generated using the DDX4-Cre and flox/flox fzr1 mouse lines and initial gross morphological analysis indicated that at 7 weeks of age null mice possessed significantly smaller testes (21.81mg ± 0.23mg) when compared to heterozygote (99.86mg ± 1.58mg) and wildtype littermates (93.06mg ± 1.16mg) n = 3 P < 0.0001. Quantitative gene expression analysis confirmed almost complete absence of fzr1 transcript in testes (20-fold decrease) in comparison to wild-type. Immunoblotting and immunohistochemistry revealed no expression of Fzr1 protein in meiotic and post meiotic germ cells when compared to heterozygote and wild type littermates. Histomorphological analysis of testes tissue sections revealed Fzr1 null males exhibited spermatogenic arrest and a complete absence of round spermatids with concomitant apoptosis in the residual spermatocytes. Epididymal examination confirmed a complete lack of mature spermatozoa in the cauda epididymis of null males. In contrast, both wild type and heterozygote mice displayed normal spermatogenesis and epididymal sperm analysis indicated no distinguishable differences in seminal characteristics with normal motility, morphology and sperm-zona binding capacity. Based on these observations we hypothesise that Fzr1 plays a significant role in the establishment and maintenance of meiosis possibly through regulation of key cell cycle proteins.

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Expression and prognostic role of Spy1 as a novel cell cycle protein in hepatocellular carcinoma

Experimental and Molecular Pathology ◽

10.1016/j.yexmp.2009.07.011 ◽

2009 ◽

Vol 87 (3) ◽

pp. 167-172 ◽

Cited By ~ 29

Author(s):

Qing Ke ◽

Juling Ji ◽

Chun Cheng ◽

Yixin Zhang ◽

Mudan Lu ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Cell Cycle ◽

Prognostic Role ◽

Cell Cycle Protein

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PTPRT Could Be a Treatment Predictive and Prognostic Biomarker for Breast Cancer

BioMed Research International ◽

10.1155/2021/3301402 ◽

2021 ◽

Vol 2021 ◽

pp. 1-23

Author(s):

Lun Li ◽

Feng Xu ◽

Pingfang Xie ◽

Liqin Yuan ◽

Meirong Zhou

Keyword(s):

Breast Cancer ◽

Cell Cycle ◽

Acquired Resistance ◽

Age Groups ◽

Breast Cancer Patients ◽

Clinical Role ◽

Primary Resistance ◽

Free Survival ◽

Predictive And Prognostic Biomarker

The role of PTPRT in breast cancer was not comprehensively explored and well analyzed. Our study comprehensively searched available databases to analyze the clinical role of PTPRT in breast cancer. We found PTPRT was an antioncogene and could be used to distinguish different stages, age groups, molecular types, and grades for breast cancer. PTPRT might be primary resistance biomarkers for taxane, anthracycline, and ixabepilone but not be acquired resistance biomarkers. Higher PTPRT expression levels were associated with longer overall survival and recurrence-free survival. PTPRT was negatively associated with Ki67 and CDK4/6 but positively associated with BCL-2. PTPRT might be associated with cell cycle and microtubule, and tumor infiltration in B cell and macrophage cell. PTPRT could predict chemotherapy effectiveness and prognosis for breast cancer patients. PTPRT might inhibit tumor growth via disrupting the microtubule dynamics and cell cycle in breast cancer.

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