Simultaneous identification and DNA barcoding of six Eimeria species infecting turkeys using PCR primers targeting the mitochondrial cytochrome c oxidase subunit I (mtCOI) locus

2015 ◽  
Vol 114 (5) ◽  
pp. 1761-1768 ◽  
Author(s):  
Mian A. Hafeez ◽  
Srichaitanya Shivaramaiah ◽  
Kristi Moore Dorsey ◽  
Mosun E. Ogedengbe ◽  
Shiem El-Sherry ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Eimeria Species ◽  
Pcr Primers ◽  
Mitochondrial Cytochrome ◽  
Oxidase Subunit ◽  
Simultaneous Identification ◽  
Mitochondrial Cytochrome C

Can DNA barcoding be used to identify closely related Clunio Haliday, 1855 species (Diptera: Chironomidae, Orthocladiinae)?

Zootaxa ◽  
2021 ◽  
Vol 4927 (1) ◽  
pp. 1-8
Author(s):  
PARASKEVA MICHALOVA ◽  
VALERIA LENCIONI ◽  
MOMCHIL NENOV ◽  
SVETOSLAV NIKOLOV
Keyword(s):  
Cytochrome C ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Methodological Approach ◽  
Dna Barcode ◽  
Mitochondrial Cytochrome ◽  
Base Pairs ◽  
Oxidase Subunit ◽  
Gene Coi ◽  
Mitochondrial Cytochrome C

DNA barcoding based on a fragment of mitochondrial Cytochrome C Oxidase subunit 1 gene (COI) was applied to the two chironomids Clunio balticus Heimbach (690 base pairs) and C. ponticus Michailova (691 base pairs). The two species differed by one deletion in the nucleotide sequence Adenine. However, the 658-nucleotide long sequences of DNA from the mitochondrial Cytochrome C Oxidase subunit 1 gene (COI) of C. balticus and C. ponticus were identical upon comparison. Further, they compared with homologous sequences for C. marinus Holiday and C. tsushimensis Tokunaga from the Barcode of Life (BOLD) database and the results plotted as a weighted graph, where C. tsushimensis, C. marinus and C. balticus C. ponticus formed three almost equidistant groups. From this, we established that the genetic distance between the respective COI sequences of C. balticus and C. ponticus is minimal, indicating a close relationship between the species indicative of recent common origin. However, the comparative analysis between C. tsushimensis, C. marinus, C. balticus and C. ponticus showed a wider divergence in their respective nucleotide sequences. Overall, our results emphasized that the COI region does not work well as a DNA barcode to identify species within the Clunio genus. Either longer sequences or a multifaceted methodological approach, including morphology, cytogenetic and ecology is needed to distinguish some members of Clunio genus.  

scholarly journals Taxonomy and the DNA Barcoding Enterprise

Zootaxa ◽  
2011 ◽  
Vol 2742 (1) ◽  
pp. 67 ◽  
Author(s):  
MALTE C. EBACH
Keyword(s):  
Cytochrome C ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Type Specimen ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Oxidase Subunit ◽  
Mitochondrial Cytochrome C

DNA Barcoding is elusive to many taxonomists. Like the numbers in a barcode, barcoding attempts to link a type specimen with a part of its DNA, most commonly from the mitochondrial Cytochrome c Oxidase subunit I (COI) gene.

Redescription of Agistemus lobatus Ehara, 1964 and A. terminalis (Quayle, 1912) (Acari: Trombidiformes: Stigmaeidae) with DNA barcoding

2019 ◽  
Vol 24 (1) ◽  
pp. 33
Author(s):  
Mohamed W. Negm ◽  
Tetsuo Gotoh
Keyword(s):  
Cytochrome C ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Dna Barcode ◽  
Predatory Mites ◽  
Mitochondrial Cytochrome ◽  
Morphological Variations ◽  
Oxidase Subunit ◽  
Mitochondrial Cytochrome C

Agistemus lobatus Ehara, 1964 and A. terminalis (Quayle, 1912) (Stigmaeidae) are cosmopolitan predatory mites and appear on various plants in Japan. They are so close morphologically and can be mainly separated based on the lengths of dorsal setae sci and c2. The present study aimed to redescribe these two species for detailed morphological variations and generate sequences of partial mitochondrial cytochrome c oxidase subunit I (COI) as DNA barcode data. Our results with DNA barcoding and morphology well separated A. lobatus from the close species A. terminalis.

scholarly journals Platelet mitochondrial cytochrome c oxidase subunit I variants with benzene poisoning

2018 ◽  
Vol 10 (12) ◽  
pp. 6811-6818 ◽  
Author(s):  
Dianpeng Wang ◽  
Xiangli Yang ◽  
Yanfang Zhang ◽  
Dafeng Lin ◽  
Paimao Li ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Mitochondrial Cytochrome ◽  
Oxidase Subunit ◽  
Mitochondrial Cytochrome C
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