Characterization of a novel Vibrio parahaemolyticus host-phage pair and antibacterial effect against the host

2022 ◽  
Author(s):  
Chao Gao ◽  
Xiaobo Yang ◽  
Chen Zhao ◽  
Chenyu Li ◽  
Shang Wang ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Antibacterial Effect

scholarly journals Characterization of a novel endolysin from bacteriophage infecting Vibrio parahaemolyticus, vB_VpaP_KF2

2020 ◽  
Vol 63 (1) ◽  
Author(s):  
Jeong-A Lim ◽  
Nari Lee ◽  
Hyang-Sook Chun ◽  
Hyun-Joo Chang
Keyword(s):  
Vibrio Parahaemolyticus

scholarly journals Characterization of Antimicrobial Resistance and Virulence Profile of Vibrio Parahaemolyticus in Water and Bivalve Mollusck Samples

2014 ◽  
Author(s):  
Norma Suely Evangelista-Barreto ◽  
Irana Paim Silva ◽  
Carla da Silva Silveira ◽  
Leopoldo Melo Barreto ◽  
Oscarina Viana de Sousa
Keyword(s):  
Antimicrobial Resistance ◽  
Vibrio Parahaemolyticus

scholarly journals Enhancement of UV Light Sensitivity of a Vibrio parahaemolyticus O3:K6 Pandemic Strain Due to Natural Lysogenization by a Telomeric Phage

2009 ◽  
Vol 75 (6) ◽  
pp. 1697-1702 ◽  
Author(s):  
Beatriz Zabala ◽  
Katherine García ◽  
Romilio T. Espejo
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Uv Light ◽  
Bacterial Pathogen ◽  
Light Sensitivity ◽  
Alkaline Extraction ◽  
Clonal Population ◽  
Pandemic Strain ◽  
Clonal Strain ◽  
Similar Restriction

ABSTRACT The Vibrio parahaemolyticus O3:K6 pandemic clonal strain was first observed in southern Chile in 2004 and has since caused approximately 8,000 seafood-related diarrhea cases in this region. The massive proliferation of the original clonal population offers a unique opportunity to study the evolution of a bacterial pathogen in its natural environment by detection and characterization of emerging bacterial variants. Here, we describe a group of pandemic variants characterized by the presence of a 42-kb extrachromosomal DNA that can be recovered by alkaline extraction. Upon treatment with mitomycin C, these variants lyse with production of a myovirus containing DNA of equal size to the plasmid but which cannot be recovered by alkaline extraction. Plasmid and phage DNAs show similar restriction patterns corresponding to enzyme sites in a circular permutation. Sequenced regions showed 81 to 99% nucleotide similarity to bacteriophage VHML of Vibrio harveyi. Altogether these observations indicate that the 42-kb plasmid corresponds to a prophage, consisting of a linear DNA with terminal hairpins of a telomeric temperate phage with a linear genome. Bacteria containing the prophage were 7 to 15 times more sensitive to UV radiation, likely due to phage induction by UV irradiation as plasmid curing restored the original sensitivity. The enhanced UV sensitivity could have a significant role in reducing the survival and propagation capability of the V. parahaemolyticus pandemic strain in the ocean.

scholarly journals Characterization of Mixed-Species Biofilm Formed by Vibrio parahaemolyticus and Listeria monocytogenes

2019 ◽  
Vol 10 ◽  
Author(s):  
Ping Chen ◽  
Jing Jing Wang ◽  
Bin Hong ◽  
Ling Tan ◽  
Jun Yan ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Vibrio Parahaemolyticus ◽  
Mixed Species

scholarly journals Genomic characterization of Vibrio parahaemolyticus from Pacific white shrimp and rearing water in Malaysia reveals novel sequence types and structural variation in genomic regions containing the Photorhabdus insect-related (Pir) toxin-like genes

2019 ◽  
Vol 366 (17) ◽  
Author(s):  
Chrystine Zou Yi Yan ◽  
Christopher M Austin ◽  
Qasim Ayub ◽  
Sadequr Rahman ◽  
Han Ming Gan
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Illumina Miseq ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Phylogenomic Analysis ◽  
Genomic Characterization ◽  
Genomic Resource ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Genomic Regions

ABSTRACT The Malaysian and global shrimp aquaculture production has been significantly impacted by acute hepatopancreatic necrosis disease (AHPND) typically caused by Vibrio parahaemolyticus harboring the pVA plasmid containing the pirAVp and pirBVp genes, which code for Photorhabdus insect-related (Pir) toxin. The limited genomic resource for V. parahaemolyticus strains from Malaysian aquaculture farms precludes an in-depth understanding of their diversity and evolutionary relationships. In this study, we isolated shrimp-associated and environmental (rearing water) V. parahaemolyticus from three aquaculture farms located in Northern and Central Malaysia followed by whole-genome sequencing of 40 randomly selected isolates on the Illumina MiSeq. Phylogenomic analysis and multilocus sequence typing (MLST) reveal distinct lineages of V. parahaemolyticus that harbor the pirABVp genes. The recovery of pVA plasmid backbone devoid of pirAVp or pirABVp in some V. parahaemolyticus isolates suggests that the toxin genes are prone to deletion. The new insight gained from phylogenomic analysis of Asian V. parahaemolyticus, in addition to the observed genomic instability of pVa plasmid, will have implications for improvements in aquaculture practices to diagnose, treat or limit the impacts of this disease.

scholarly journals Prevalence, antibiotic susceptibility and characterization of Vibrio parahaemolyticus isolates in China

2020 ◽  
Vol 367 (16) ◽  
Author(s):  
Tengfei Xie ◽  
Quan Yu ◽  
Xiong Tang ◽  
Jing Zhao ◽  
Xujun He
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Antimicrobial Agents ◽  
Consensus Sequence ◽  
Multidrug Resistant ◽  
Food Samples ◽  
Retail Markets ◽  
Aquatic Product ◽  
Resistance Patterns ◽  
Maximum Proportion

Abstract Vibrio parahaemolyticus is a marine and estuarine bacterium that poses a major threat to human health worldwide. In this study, from 2017 to 2019, we evaluated 900 food samples collected from China in 2017, with the aim of determining the incidence and features of V. parahaemolyticus in ready-to-eat (RTE) foods, shrimp and fish in China. The contamination rates in these were 3.67, 19.33 and 10.67%, respectively, and the prevalence of V. parahaemolyticus was higher in summer than in winter. In addition, 101 V. parahaemolyticus strains were isolated. Our results suggested that most of the isolates were resistant to aminoglycosides based on the antimicrobial resistance patterns of these aquatic product isolates against 14 antimicrobial agents. Furthermore, most of the isolates were multidrug-resistant. Serotyping showed that the isolates of the O2 serotype comprised the maximum proportion. Enterobacterial repetitive intergenic consensus sequence (ERIC)-PCR results indicated that the isolates (n = 101) could be classified into 12 clusters. There were 82 STs suggesting genetic variation and relatedness among these isolates. Our findings demonstrated the presence of V. parahaemolyticus in foods from Chinese retail markets and show that this methodology can be used for microbiological risk assessment in China.

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