Effects of high-frequency near-infrared diode laser irradiation on the proliferation and migration of mouse calvarial osteoblasts

2018 ◽  
Vol 33 (5) ◽  
pp. 959-966 ◽  
Author(s):  
Ryo Kunimatsu ◽  
Hidemi Gunji ◽  
Yuji Tsuka ◽  
Yuki Yoshimi ◽  
Tetsuya Awada ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Diode Laser ◽  
High Frequency ◽  
Near Infrared ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals High-Frequency Near-Infrared Diode Laser Irradiation Attenuates IL-1β-Induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes

2020 ◽  
Vol 9 (3) ◽  
pp. 881 ◽  
Author(s):  
Shuzo Sakata ◽  
Ryo Kunimatsu ◽  
Yuji Tsuka ◽  
Ayaka Nakatani ◽  
Tomoka Hiraki ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Protein Expression ◽  
Laser Irradiation ◽  
Diode Laser ◽  
High Frequency ◽  
Near Infrared ◽  
Mrna Levels ◽  
Induced Expression ◽  
Tnf Α

High-frequency near-infrared diode laser provides a high-peak output, low-heat accumulation, and efficient biostimulation. Although these characteristics are considered suitable for osteoarthritis (OA) treatment, the effect of high-frequency near-infrared diode laser irradiation in in vitro or in vivo OA models has not yet been reported. Therefore, we aimed to assess the biological effects of high-frequency near-infrared diode laser irradiation on IL-1β-induced chondrocyte inflammation in an in vitro OA model. Normal Human Articular Chondrocyte-Knee (NHAC-Kn) cells were stimulated with human recombinant IL-1β and irradiated with a high-frequency near-infrared diode laser (910 nm, 4 or 8 J/cm2). The mRNA and protein expression of relevant inflammation- and cartilage destruction-related proteins was analyzed. Interleukin (IL) -1β treatment significantly increased the mRNA levels of IL-1β, IL-6, tumor necrosis factor (TNF) -α, matrix metalloproteinases (MMP) -1, MMP-3, and MMP-13. High-frequency near-infrared diode laser irradiation significantly reduced the IL-1β-induced expression of IL-1β, IL-6, TNF-α, MMP-1, and MMP-3. Similarly, high-frequency near-infrared diode laser irradiation decreased the IL-1β-induced increase in protein expression and secreted levels of MMP-1 and MMP-3. These results highlight the therapeutic potential of high-frequency near-infrared diode laser irradiation in OA.

Effect of high-frequency near-infrared diode laser irradiation on periodontal tissues during experimental tooth movement in rats

2018 ◽  
Vol 50 (7) ◽  
pp. 772-780 ◽  
Author(s):  
Hidemi Gunji ◽  
Ryo Kunimatsu ◽  
Yuji Tsuka ◽  
Yuki Yoshimi ◽  
Keisuke Sumi ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Diode Laser ◽  
High Frequency ◽  
Near Infrared ◽  
Tooth Movement ◽  
Periodontal Tissues ◽  
Experimental Tooth Movement

scholarly journals Loss of alanine-glyoxylate and serine-pyruvate aminotransferase expression accelerated the progression of hepatocellular carcinoma and predicted poor prognosis

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Yufeng Sun ◽  
Wenchao Li ◽  
Shiqi Shen ◽  
Xuejing Yang ◽  
Bing Lu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Systematic Review ◽  
Cell Line ◽  
Cdna Microarray ◽  
High Frequency ◽  
Critical Role ◽  
Differentially Expressed ◽  
And Migration ◽  
Proliferation And Migration

Abstract Background Accumulated studies reported abnormal gene expression profiles of hepatocellular carcinoma (HCC) by cDNA microarray. We tried to merge cDNA microarray data from different studies to search for stably changed genes, and to find out better diagnostic and prognostic markers for HCC. Methods A systematic review was performed by searching publications indexed in Pubmed from March 1, 2001 to July 1, 2016. Studies that reporting cDNA microarray profiles in HCC, containing both tumor and nontumor data and published in English-language were retrieved. The differentially expressed genes from eligible studies were summarized and ranked according to the frequency. High frequency genes were subjected to survival analyses. The expression and prognostic value of alanine-glyoxylate and serine-pyruvate aminotransferase (AGXT) was further evaluated in HCC datasets in Oncomine and an independent HCC tissue array cohort. The role of AGXT in HCC progression was evaluated by proliferation and migration assays in a human HCC cell line. Results A total of 43 eligible studies that containing 1917 HCC patients were included, a list of 2022 non redundant abnormally expressed genes in HCC were extracted. The frequencies of reported genes were ranked. We finally obtained a list of only five genes (AGXT; ALDOB; CYP2E1; IGFBP3; TOP2A) that were differentially expressed in tumor and nontumor tissues across studies and were significantly correlated to HCC prognosis. Only AGXT had not been reported in HCC. Reduced expression of AGXT reflected poor differentiation of HCC and predicts poor survival. Knocking down of AGXT enhanced cell proliferation and migration of HCC cell line. Conclusions The present study supported the feasibility and necessity of systematic review on discovering new and reliable biomarkers for HCC. We also identified a list of high frequency prognostic genes and emphasized a critical role of AGXT deletion during HCC progression.

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