scholarly journals Enhancement of vincristine under in vitro culture of Catharanthus roseus supplemented with Alternaria sesami endophytic fungal extract as a biotic elicitor

2021 ◽  
Author(s):  
Kanchan Birat ◽  
Tariq Omar Siddiqi ◽  
Showkat Rasool Mir ◽  
Junaid Aslan ◽  
Rakhi Bansal ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Catharanthus Roseus ◽  
Fungal Extract ◽  
Biotic Elicitor

scholarly journals “Enhancement of Vincristine Under In Vitro Culture of Catharanthus Roseus Supplemented With Alternaria Sesami Endophytic Fungal Extract As Biotic Elicitor”

2021 ◽  
Author(s):  
Kanchan Birat ◽  
Tariq Omar Siddiqi ◽  
Showkat Rasool Mir ◽  
Junaid Aslan ◽  
Rakhi Bansal ◽  
...  
Keyword(s):  
Catharanthus Roseus ◽  
High Performance ◽  
Vinca Alkaloid ◽  
Fungal Endophyte ◽  
Fungal Culture ◽  
Fungal Extract ◽  
Biotic Elicitor ◽  
Culture Extract ◽  
First Time

Abstract Vincristine, one of the major vinca alkaloid of Catharanthus roseus(L.) G. Don. (Apocynaceae) was enhanced under in vitro culture of C.roseus using fungal extract of an endophyte Alternaria sesami isolated from the surface-sterilized root cuttings of C.roseus. Vindoline, a precursor molecule of Vincristine was detected for the first time from the fungal endophyte A.sesami which was used as biotic elicitor to enhance Vincristine content in the C.roseus callus.It was identified using high performance liquid chromatography and mass spectroscopy techniques by matching retention time and mass data with reference molecule. Supplementing heat sterilized A.sesami endophytic fungal culture extract into callus culture medium of C. roseus enhanced the Vincristine content in C. roseus callus by 21.717% after 105 day culture.

scholarly journals The induction and development of somatic embryos from the in vitro culture of Catharanthus roseus (L.) G. Don

2021 ◽  
Vol 35 (1) ◽  
Author(s):  
H. Farhadi ◽  
M.B. Hassanpouraghdam ◽  
Mohammad Ali Aazami
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Culture ◽  
Catharanthus Roseus ◽  
Somatic Embryos ◽  
Leaf Explants ◽  
Fresh Weight ◽  
Efficient Treatment ◽  
Hypocotyl Explants ◽  
Important Medicinal Plant

Catharanthus roseus is containing anticancer alkaloids vincristine and vinblastine as is an important medicinal plant. Several studies have conducted on in-vitro culture of this plant. To optimize the somatic embryogenesis, a factorial based on CRD experiment with 10 replications was conducted. Root, hypocotyl and leaf explants grown in-vitro were transferred and cultured on MS media containing different combinations of 2,4-D, NAA and 2,4-D×BAP. The results revealed that in callogenesis, the interaction effects of root and hypocotyl explants×2,4-D and NAA as well as hypocotyl×(1 mg l-1 NAA+1 mg l-1 BAP) was superior than other treatments (p≤0.01). For calli fresh weight, hypocotyl×NAA and hypocotyl×(1 mg l-1 NAA+1 mg l-1 BAP) was the treatment of choice (p≤0.01). The calli produced were sub-cultured to attain the pre-embryos and somatic embryos. For the number of pre-embryos and somatic embryos; the interaction of hypocotyl×2,4-D was the best and most efficient treatment. Seemingly, the production of somatic embryos is accessible in this plant by the logical management of growth regulator combinations. Furthermore, the production and genetic engineering of the somatic embryos could be a promising trend in the subsequent production of high-valued metabolites from this plant.

Isohexenylnaphthazarins from Lithospermum canescens (Michx.) Lehm. and Arnebia euchroma (Royle) Jonst. in vitro culture

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
K Graikou ◽  
H Damianakos ◽  
K Syklowska-Baranek ◽  
A Pietrosiuk ◽  
M Jeziorek ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Arnebia Euchroma ◽  
Lithospermum Canescens

Effect of antioxidants on in vitro culture of pomegranate cv. Sindhuri

2018 ◽  
Vol 34 (2) ◽  
pp. 311-318
Author(s):  
Ravi Kumar ◽  
◽  
M.L. Jakhar ◽  
Komal Sekhawat ◽  
Swarnlata Kumawat ◽  
...  
Keyword(s):  
In Vitro Culture

Leaf Color of Plants Regenerated through In Vitro Culture from Variegated Leaf Segments of Caladium.

1993 ◽  
Vol 62 (3) ◽  
pp. 619-624 ◽  
Author(s):  
Yu Zhu ◽  
Tetsuyuki Takemoto ◽  
Susumu Yazawa
Keyword(s):  
In Vitro Culture ◽  
Leaf Color ◽  
Leaf Segments ◽  
Variegated Leaf
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