Identification of wheat-Agropyron cristatum 6P translocation lines and localization of 6P-specific EST markers

Euphytica ◽  
2015 ◽  
Vol 208 (2) ◽  
pp. 265-275 ◽  
Author(s):  
Cheng Dai ◽  
Ainong Gao
Keyword(s):  
Agropyron Cristatum ◽  
Translocation Lines

scholarly journals Development and Characterization of Wheat-Agropyron cristatum Introgression Lines Induced by Gametocidal Genes and Wheat ph1b Mutant

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 277
Author(s):  
Alejandro Copete-Parada ◽  
Carmen Palomino ◽  
Adoración Cabrera
Keyword(s):  
Genetic Resources ◽  
Agropyron Cristatum ◽  
Homologous Gene ◽  
Aegilops Cylindrica ◽  
Induction Frequency ◽  
Structural Aberrations ◽  
Translocation Lines ◽  
New Germplasm

The P genome of Agropyron cristatum Gaertn. contains many desirable genes that can be utilized as genetic resources to improve wheat. In this research, we used both the gametocidal chromosome 2Cc and the pairing homologous gene (Ph1b) mutant to induce structural aberrations and translocations between wheat and the 4P, 5P, and 6P genome chromosomes. By using the two approaches, a total of 19 wheat-A. cristatum translocations have been identified, in which 13 were induced by the Triticum aestivum cv. Chinese Spring (CS) ph1b mutant (CS ph1b) and six were induced by gametocidal chromosome 2Cc from Aegilops cylindrica Host. The wheat-4P, -5P and -6P A. cristatum translocations were characterized by in situ hybridization and by a set of conserved orthologous set (COS) molecular markers. The aberrations included centromeric translocations, terminal translocations, dicentric translocations, and deletions. The average induction frequency of chromosome structural aberrations was 10.9% using gametocidal 2Cc chromosome and 8.8% using ph1b mutant. The highest frequency obtained was for chromosome 4P using both approaches. All the wheat-A. cristatum translocation lines obtained were valuable for identifying A. cristatum chromosome 4P, 5P, and 6P related genes. In addition, these lines provided genetic resources and new germplasm accessions for the genetic improvement of wheat.

Production and identification of wheat-Agropyron cristatum 6P translocation lines

Planta ◽  
2010 ◽  
Vol 232 (2) ◽  
pp. 501-510 ◽  
Author(s):  
Yang Luan ◽  
Xiaoguang Wang ◽  
Weihua Liu ◽  
Chunye Li ◽  
Jinpeng Zhang ◽  
...  
Keyword(s):  
Agropyron Cristatum ◽  
Translocation Lines

scholarly journals Production and Identification of Wheat-Agropyron cristatum 2P Translocation Lines

PLoS ONE ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. e0145928 ◽  
Author(s):  
Huanhuan Li ◽  
Mingjie Lv ◽  
Liqiang Song ◽  
Jinpeng Zhang ◽  
Ainong Gao ◽  
...  
Keyword(s):  
Agropyron Cristatum ◽  
Translocation Lines

Cytological and molecular analysis of wheat – Agropyron cristatum translocation lines with 6P chromosome fragments conferring superior agronomic traits in common wheat

Genome ◽  
2016 ◽  
Vol 59 (10) ◽  
pp. 840-850 ◽  
Author(s):  
Liqiang Song ◽  
Yuqing Lu ◽  
Jinpeng Zhang ◽  
Cuili Pan ◽  
Xinming Yang ◽  
...  
Keyword(s):  
Common Wheat ◽  
Agronomic Traits ◽  
Physical Map ◽  
Wheat Breeding ◽  
Agropyron Cristatum ◽  
Grain Number ◽  
Specific Sequence ◽  
Sts Markers ◽  
Grain Number Per Spike ◽  
Translocation Lines

Agropyron cristatum (2n = 4x = 28, PPPP) is a wild relative of common wheat and confers several desirable agronomic traits to wheat, such as high grain number per spike and enhanced resistance to certain diseases. Development of wheat – A. cristatum 6P translocation lines facilitates its utilization in wheat improvement. In this study, 26 wheat – A. cristatum 6P translocation lines were characterized by in situ hybridization (ISH) and 6P-specific sequence-tagged-site (STS) markers. These translocation lines carried different 6P chromosomal segments, which covered the whole 6P chromosome. FISH results showed that 15, 5, and 6 lines were translocated onto wheat A, B, and D genomes, respectively. Compared with the previous reports, a fine physical map of 6P chromosome was constructed, consisting of 31 chromosomal bins with 255 STS markers. Twelve translocation lines containing 6PS13∼14 chromosomal bins were highly resistant to leaf rust. Two lines showed high grain number per spike, and three lines displayed both enhanced grain number per spike and thousand-grain weight. Development of wheat – A. cristatum 6P translocation lines will not only provide novel wheat germplasm for wheat breeding but also be helpful to broaden the genetic basis of common wheat.

scholarly journals Efficient Induction of Wheat-Agropyron cristatum 6P Translocation Lines and GISH Detection

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e69501 ◽  
Author(s):  
Liqiang Song ◽  
Lili Jiang ◽  
Haiming Han ◽  
Ainong Gao ◽  
Xinming Yang ◽  
...  
Keyword(s):  
Agropyron Cristatum ◽  
Efficient Induction ◽  
Translocation Lines

Development and characterization of novel Triticum aestivum-Agropyron cristatum 6P Robertsonian translocation lines

2021 ◽  
Vol 41 (10) ◽  
Author(s):  
Kai Qi ◽  
Haiming Han ◽  
Jinpeng Zhang ◽  
Shenghui Zhou ◽  
Xiuquan Li ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Robertsonian Translocation ◽  
Agropyron Cristatum ◽  
Translocation Lines

Production and identification of wheat – Agropyron cristatum (1·4P) alien translocation lines

Genome ◽  
2010 ◽  
Vol 53 (6) ◽  
pp. 472-481 ◽  
Author(s):  
Wei-Hua Liu ◽  
Yang Luan ◽  
Jing-Chang Wang ◽  
Xiao-Guang Wang ◽  
Jun-Ji Su ◽  
...  
Keyword(s):  
Molecular Markers ◽  
In Situ Hybridization ◽  
Genetic Resources ◽  
Agropyron Cristatum ◽  
Chromosome 1 ◽  
Addition Line ◽  
Deletion Lines ◽  
Chromosome Fragments ◽  
Translocation Lines

The P genome of Agropyron Gaertn., a wild relative of wheat, contains an abundance of desirable genes that can be utilized as genetic resources to improve wheat. In this study, wheat – Aegilops cylindrica Host gametocidal chromosome 2C addition lines were crossed with wheat – Agropyron cristatum (L.) Gaertn. disomic addition line accession II-21 with alien recombinant chromosome (1·4)P. We successfully induced wheat – A. cristatum alien chromosomal translocations for the first time. The frequency of translocation in the progeny was 3.75%, which was detected by molecular markers and genomic in situ hybridization (GISH). The translocation chromosomes were identified by dual-color GISH /fluorescence in situ hybridization (FISH). The P genomic DNA was used as probe to detect the (1·4)P chromosome fragment, and pHvG39, pAs1, or pSc119.2 repeated sequences were used as probes to identify wheat translocated chromosomes. The results showed that six types of translocations were identified in the three wheat – A. cristatum alien translocation lines, including the whole arm or terminal portion of a (1·4)P chromosome. The (1·4)P chromosome fragments were translocated to wheat chromosomes 1B, 2B, 5B, and 3D. The breakpoints were located at the centromeres of 1B and 2B, the pericentric locations of 5BS, and the terminals of 5BL and 3DS. In addition, we obtained 12 addition–deletion lines that contained alien A. cristatum chromosome (1·4)P in wheat background. All of these wheat – A. cristatum alien translocation lines and addition–deletion lines would be valuable for identifying A. cristatum chromosome (1·4)P-related genes and providing genetic resources and new germplasm accessions for the genetic improvement of wheat. The specific molecular markers of A. cristatum (1·4)P chromosome have been developed and used to track the (1·4)P chromatin.

Evaluation of mineral fertilizer effects in a long-term experiment on a planted sward

2010 ◽  
Vol 59 (2) ◽  
pp. 295-314 ◽  
Author(s):  
Imre Kádár
Keyword(s):  
Festuca Arundinacea ◽  
Dactylis Glomerata ◽  
Mineral Fertilizer ◽  
Phleum Pratense ◽  
Agropyron Cristatum ◽  
Festuca Rubra ◽  
Festuca Pratensis ◽  
Term Experiment ◽  
Long Term Experiment

Egy műtrágyázási tartamkísérlet 32. évében, 2005-ben vizsgáltuk az eltérő N-, P- és K-ellátottsági szintek és kombinációik hatását a réti csenkesz (Festuca pratensis) vezérnövényű, nyolckomponensű, pillangós nélküli gyepkeverék termésére, fejlődésére és elemtartalmára. A termőhely mészlepedékes csernozjom talaja a szántott rétegben mintegy 3% humuszt, 3–5% CaCO3-ot és 20–22% agyagot tartalmazott, N és K elemekben közepesen, P és Zn elemekben gyengén ellátottnak minősült. A kísérlet 4N×4P×4K = 64 kezelést×2 ismétlést = 128 parcellát foglalt magában. A talajvíz 13–15 m mélyen helyezkedik el, a terület aszályérzékeny. A vizsgált 2005. évben azonban kielégítő mennyiségű (649 mm) csapadék hullott és annak eloszlása is kedvező volt. A gyep telepítése spenót elővetemény után 2000. szeptember 20-án történt gabona sortávra 60 kg·ha–1 vetőmaggal, amelynek 25%-át (15 kg) a réti csenkesz (Festuca pratensis); 21–21%-át (12,6 kg) a nádképű csenkesz (Festuca arundinacea) és az angol perje (Lolium perenne); 9%-át (5,4 kg) a taréjos búzafű (Agropyron cristatum), valamint 6–6%-át (3,6 kg) a vörös csenkesz (Festuca rubra), a réti komócsin (Phleum pratense), a zöld pántlikafű (Phalaris arundinacea) és a csomós ebír (Dactylis glomerata) tette ki. Főbb eredményeink: – A meghatározó N-trágyázás nyomán a szénatermés 5-szörösére emelkedett a két kaszálással a N-kontrollhoz viszonyítva. A maximális 10 t·ha–1 körüli légszáraz szénahozamokat a 300 kg N·ha–1·év–1 N-adag, valamint a 150 mg·kg–1 körüli AL-P2O5-, illetve 150 mg·kg–1 feletti AL-K2O-tartalom biztosította. Növénydiagnosztikai szempontból a nagy terméshez kötődő optimális elemtartalom 2% körüli N- és K-, illetve 0,2–0,3% P-koncentráció volt a szénában. – A két kaszálással felvett minimum (a 2 t·ha–1 körüli szénatermést adó N-kontroll) és maximum (a 10 t·ha–1 körüli szénahozamú, nitrogénnel és PK-vel jól ellátott talajok) elemmennyiségek a következőképpen alakultak: N 21–196 kg, K 39–188 kg, Ca 9–48 kg, Mg 4–22 kg, P 6–21 kg. – Az N×P és N×K kölcsönhatások kifejezettebbé váltak a 2. kaszálás idején. A P 0,18–0,55%, a NO3-N 86–1582 mg·kg–1, a Cu 4,7–7,4 mg·kg–1, a Mo 0,7–4,1 mg·kg–1 extrém értékeket jelzett az N×P kezelések függvényében. Az N×K kezelésekben a K 1,44–2,73%, a Mg 0,26–0,39%, a Na 71–2178 mg·kg–1, a Ba 4,1–9,6 mg·kg–1, a Cd 15–44 µg·kg–1 szélsőértékekkel volt jellemezhető. A Sr a 10–26 mg·kg–1 koncentrációtartományban módosult a P×K-ellátottság nyomán. Élettani, takarmányozástani szempontból az indukált kölcsönhatások nyomon követése elengedhetetlen, amennyiben olyan mérvű tápelemhiányok, illetve aránytalanságok jöhetnek létre, melyek anyagcserezavarokat okozhatnak a növényt fogyasztó állatban.

Development of EST Markers Specific to Agropyron cristatum Chromosome 6P in Common Wheat Background

2013 ◽  
Vol 38 (10) ◽  
pp. 1791-1801 ◽  
Author(s):  
Cheng DAI ◽  
Jin-Peng ZHANG ◽  
Xiao-Yang WU ◽  
Xin-Ming YANG ◽  
Xiu-Quan LI ◽  
...  
Keyword(s):  
Common Wheat ◽  
Agropyron Cristatum ◽  
Chromosome 6P
Sign in / Sign up

Export Citation Format

Share Document