Genetic variation within and among populations of Orychophragmus violaceus (Cruciferae) in China as detected by ISSR analysis

2009 ◽  
Vol 57 (1) ◽  
pp. 55-64 ◽  
Author(s):  
Li-Jun Zhang ◽  
Si-Lan Dai
Keyword(s):  
Genetic Variation ◽  
Orychophragmus Violaceus ◽  
Issr Analysis

ISSR Analysis Reveals High Genetic Variation in Strawberry Three-Way Hybrids Developed for Tropical Regions

2021 ◽  
Author(s):  
Jessica Vanessa Wosniak Corrêa ◽  
Gabriela Gomes Weber ◽  
André Ricardo Zeist ◽  
Juliano Tadeu Vilela de Resende ◽  
Paulo Roberto Da-Silva
Keyword(s):  
Genetic Variation ◽  
Tropical Regions ◽  
Issr Analysis

Genetic variation within the endangered quillwort Isoëtes hypsophila (Isoetaceae) in China as evidenced by ISSR analysis

2005 ◽  
Vol 82 (2) ◽  
pp. 89-98 ◽  
Author(s):  
Jin-Ming Chen ◽  
Xing Liu ◽  
Jing-Yuan Wang ◽  
Gituru Wahiti Robert ◽  
Qing-Feng Wang
Keyword(s):  
Genetic Variation ◽  
Issr Analysis

Inter-simple sequence repeat (ISSR) analysis of genetic variation of Chondrus crispus populations from North Atlantic

2008 ◽  
Vol 88 (2) ◽  
pp. 154-159 ◽  
Author(s):  
Xiuliang Wang ◽  
Fengjuan Zhao ◽  
Zimin Hu ◽  
Alan T. Critchley ◽  
Steve L. Morrell ◽  
...  
Keyword(s):  
Genetic Variation ◽  
North Atlantic ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Chondrus Crispus ◽  
Sequence Repeat ◽  
Issr Analysis ◽  
Simple Sequence

scholarly journals Use of Intersimple Sequence Repeat Assay for Detection of DNA Polymorphism Induced by Gamma Rays in Curcuma alismatifolia

HortScience ◽  
2013 ◽  
Vol 48 (11) ◽  
pp. 1346-1351 ◽  
Author(s):  
Sima Taheri ◽  
Thohirah Lee Abdullah ◽  
Nur Ashikin Psyquay Abdullah ◽  
Zaiton Ahmad
Keyword(s):  
Genetic Variation ◽  
Genetic Similarity ◽  
Dna Polymorphism ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
The Other ◽  
Sequence Repeat ◽  
Induced Mutations ◽  
Similarity Coefficients ◽  
Issr Analysis

In plant breeding programs with vegetatively propagated plants, induced mutations with low physiological effects and strong genetic effects are important approaches for broadening the genetic variation. In the present study, intersimple sequence repeat (ISSR) assays were used to identify DNA polymorphism among the mutant varieties of Curcuma alismatifolia (Chiang Mai Red, Sweet Pink, Kimono Pink) and one hybrid (Doi Tung 554) exposed to 0-, 10-, and 20-Gy optimum doses of acute gamma irradiation. To identify DNA polymorphism among the mutants (10 and 20 Gy) and non-treated (0 Gy) samples through ISSR assay, a total of 25 random primers were screened and 14 gave reproducible polymorphic bands. The number of presumed alleles revealed by the ISSR analysis ranged from three to 19 alleles with mean values of 6.5, 7.8, and 9.2 alleles per locus for doses of 0 (control), 10, and 20 Gy, respectively. The average effective number of alleles, Nei’s gene diversity, Shannon’s information index, and polymorphic information content (PIC) were 1.41 to 1.48, 0.24 to 0.33, 0.35 to 0.45, and 0.21 to 0.25 for the three doses, respectively. Percentages of polymorphic loci for non-irradiated (0 Gy), 10 Gy, and 20 Gy individual plants were 76.4%, 83.7%, and 85.8%, respectively, with an average of 81.9%. Jaccard’s genetic similarity coefficient varied from 0.19 to 1.0 indicating the level of genetic variation among the mutants studied. In terms of variety, the range of genetic variation (0.27 to 0.4) among individuals of the Doi Tung population was more than that observed in the other three varieties. In a dendrogram constructed based on genetic similarity coefficients, the 44 C. alismatifolia individual plants fell into seven major clusters at a coefficient level of 0.35. The study revealed that DNA polymorphism detected by ISSR analysis offered a useful molecular marker for the identification of mutants in gamma radiation-treated plants. Additionally, this research demonstrated that the DNA of the hybrid (Doi Tung 554) showed a greater response in induced mutation compared with the other varieties.

scholarly journals Molecular Distinction Among Mulberry (Morus Spp.) Species and Varieties Cultivated in DPR Korea

2021 ◽  
Author(s):  
Un-Hyang Ho ◽  
Jung Sam Kye ◽  
Song Im Chae ◽  
Jong Ho Kim ◽  
Myong Ho Kim
Keyword(s):  
Genetic Variation ◽  
Internal Transcribed Spacer ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Breeding Strategy ◽  
Issr Analysis ◽  
Morus Spp ◽  
Simple Sequence ◽  
Issr Primers

Abstract Mulberry (Morus spp.) is a cross-pollinating and highly hybridized plant of which productivity are greatly varied in different varieties. We analysed molecular distinction among four mulberry species and varieties cultivated in DPR Korea by using nuclear ribosomal internal transcribed spacer (ITS) sequences and inter simple sequence repeat (ISSR) markers. ITS sequences didn’t represent a remarkable interspecific distinction among four mulberry species used in our study, suggesting that it could not be employed to identify them. ISSR analysis using 16 random primers generated 158 different markers ranging from 100 to 4000 bp in size. The results showed the inter-specific genetic variation (55.34%) was slightly higher than intra-specific genetic variation(44.66%), with comparatively low average number of migrants per generation (Nm) among populations (0.3886). Using ISSR primers selected in this study, in the future, the suitable breeding strategy might be established in raising of elite mulberry varieties on the basis of interspecific hybridization.

Genetic variation in the endemic plant Cycas debaoensis on the basis of ISSR analysis

2005 ◽  
Vol 53 (2) ◽  
pp. 141 ◽  
Author(s):  
Xie Jianguang ◽  
Jian Shuguang ◽  
Liu Nian
Keyword(s):  
Genetic Variation ◽  
Gene Flow ◽  
Population Level ◽  
Species Level ◽  
Endemic Plant ◽  
Issr Analysis ◽  
Rare And Endangered ◽  
Limited Gene Flow ◽  
High Level ◽  
Conservation And Management

Cycas debaoensis Y.C.Zhong et C.J.Chen is a rare and endangered cycad species that is endemic to South China. It has divided pinnae. Genetic variation in the species was investigated by using an intersimple sequence repeat (ISSR) marker. The species exhibits a moderate level of genetic diversity (at population level P = 34.7%, H = 0.141, I = 0.204; at species level P = 57.4%, H = 0.215, I = 0.315) and a high level of genetic differentiation among populations (Gst = 0.342). Thus, the indirect estimate of gene flow on the basis of Gst was low (Nm = 0.481). The narrow colonisation area and island pattern of distribution, combined with limited gene flow among populations and genetic drift, may play an important role in shaping the genetic structure of C. debaoensis. On the basis of these findings, strategies are suggested for the conservation and management of the species.

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