Development of a Set of Certified Reference Samples of Solid Substances with Open Porosity

Author(s):  
E. P. Sobina ◽  
I. P. Aronov
2020 ◽  
pp. 43-50
Author(s):  
Yauheniya N. Saukova

It is shown that the issues of metrological traceability for extended self-luminous objects with a wide range of brightness have not yet been resolved, since the rank scales of embedded systems are used for processing digital images. For such scales, there is no “fixed” unit, which does not allow you to get reliable results and ensure the unity of measurements. An experiment is described to evaluate the accuracy of determining the intensity (coordinates) of the color of self-luminous objects. In terms of repeatability and intermediate precision compared to the reference measurement method, the color and chromaticity coordinates of self-luminous objects (reference samples) were determined by their multiple digital registration using technical vision systems. The possibilities of the developed methodology for colorimetric studies in hardware and software environments from the point of view of constructing a multidimensional conditional scale are determined.


2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Wendell Jones ◽  
Binsheng Gong ◽  
Natalia Novoradovskaya ◽  
Dan Li ◽  
Rebecca Kusko ◽  
...  

Abstract Background Oncopanel genomic testing, which identifies important somatic variants, is increasingly common in medical practice and especially in clinical trials. Currently, there is a paucity of reliable genomic reference samples having a suitably large number of pre-identified variants for properly assessing oncopanel assay analytical quality and performance. The FDA-led Sequencing and Quality Control Phase 2 (SEQC2) consortium analyze ten diverse cancer cell lines individually and their pool, termed Sample A, to develop a reference sample with suitably large numbers of coding positions with known (variant) positives and negatives for properly evaluating oncopanel analytical performance. Results In reference Sample A, we identify more than 40,000 variants down to 1% allele frequency with more than 25,000 variants having less than 20% allele frequency with 1653 variants in COSMIC-related genes. This is 5–100× more than existing commercially available samples. We also identify an unprecedented number of negative positions in coding regions, allowing statistical rigor in assessing limit-of-detection, sensitivity, and precision. Over 300 loci are randomly selected and independently verified via droplet digital PCR with 100% concordance. Agilent normal reference Sample B can be admixed with Sample A to create new samples with a similar number of known variants at much lower allele frequency than what exists in Sample A natively, including known variants having allele frequency of 0.02%, a range suitable for assessing liquid biopsy panels. Conclusion These new reference samples and their admixtures provide superior capability for performing oncopanel quality control, analytical accuracy, and validation for small to large oncopanels and liquid biopsy assays.


Materials ◽  
2020 ◽  
Vol 14 (1) ◽  
pp. 155
Author(s):  
Régis Pamponet da Fonseca ◽  
Janaíde Cavalcante Rocha ◽  
Malik Cheriaf

The addition of natural fibers used as reinforcement has great appeal in the construction materials industry since natural fibers are cheaper, biodegradable, and easily available. In this work, we analyzed the feasibility of using the fibers of piassava, tucum palm, razor grass, and jute from the Amazon rainforest as reinforcement in mortars, exploiting the mechanical properties of compressive and flexural strength of samples with 1.5%, 3.0%, and 4.5% mass addition of the composite binder (50% Portland cement + 40% metakaolin + 10% fly ash). The mortars were reinforced with untreated (natural) and treated (hot water treatment, hornification, 8% NaOH solution, and hybridization) fibers, submitted to two types of curing (submerged in water, and inflated with CO2 in a pressurized autoclave) for 28 days. Mortars without fibers were used as a reference. For the durability study, the samples were submitted to 20 drying/wetting cycles. The fibers improved the flexural strength of the mortars and prevented the abrupt rupture of the samples, in contrast to the fragile behavior of the reference samples. The autoclave cure increased the compressive strength of the piassava and tucum palm samples with 4.5% of fibers.


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