ISSR Analysis Reveals High Genetic Variation in Strawberry Three-Way Hybrids Developed for Tropical Regions

2021 ◽  
Author(s):  
Jessica Vanessa Wosniak Corrêa ◽  
Gabriela Gomes Weber ◽  
André Ricardo Zeist ◽  
Juliano Tadeu Vilela de Resende ◽  
Paulo Roberto Da-Silva
Keyword(s):  
Genetic Variation ◽  
Tropical Regions ◽  
Issr Analysis

Genetic variation within the endangered quillwort Isoëtes hypsophila (Isoetaceae) in China as evidenced by ISSR analysis

2005 ◽  
Vol 82 (2) ◽  
pp. 89-98 ◽  
Author(s):  
Jin-Ming Chen ◽  
Xing Liu ◽  
Jing-Yuan Wang ◽  
Gituru Wahiti Robert ◽  
Qing-Feng Wang
Keyword(s):  
Genetic Variation ◽  
Issr Analysis

Schistosome genetic diversity: the implications of population structure as detected with microsatellite markers

Parasitology ◽  
2002 ◽  
Vol 125 (7) ◽  
pp. S51-S59 ◽  
Author(s):  
J. CURTIS ◽  
R. E. SORENSEN ◽  
D. J. MINCHELLA
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Variation ◽  
Molecular Markers ◽  
Microsatellite Markers ◽  
Natural Populations ◽  
Mitochondrial Marker ◽  
Tropical Regions ◽  
Blood Flukes

Blood flukes in the genus Schistosoma are important human parasites in tropical regions. A substantial amount of genetic diversity has been described in populations of these parasites using molecular markers. We first consider the extent of genetic variation found in Schistosoma mansoni and some factors that may be contributing to this variation. Recently, though, attempts have been made to analyze not only the genetic diversity but how that diversity is partitioned within natural populations of schistosomes. Studies with non-allelic molecular markers (e.g. RAPDs and mtVNTRs) have indicated that schistosome populations exhibit varying levels of gene flow among component subpopulations. The recent characterization of microsatellite markers for S. mansoni provided an opportunity to study schistosome population structure within a population of schistosomes from a single Brazilian village using allelic markers. Whereas the detection of population structure depends strongly on the type of analysis with a mitochondrial marker, analyses with a set of seven microsatellite loci consistently revealed moderate genetic differentiation when village boroughs were used to define parasite subpopulations and greater subdivision when human hosts defined subpopulations. Finally, we discuss the implications that such strong population structure might have on schistosome epidemiology.

Inter-simple sequence repeat (ISSR) analysis of genetic variation of Chondrus crispus populations from North Atlantic

2008 ◽  
Vol 88 (2) ◽  
pp. 154-159 ◽  
Author(s):  
Xiuliang Wang ◽  
Fengjuan Zhao ◽  
Zimin Hu ◽  
Alan T. Critchley ◽  
Steve L. Morrell ◽  
...  
Keyword(s):  
Genetic Variation ◽  
North Atlantic ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Chondrus Crispus ◽  
Sequence Repeat ◽  
Issr Analysis ◽  
Simple Sequence

scholarly journals Use of Intersimple Sequence Repeat Assay for Detection of DNA Polymorphism Induced by Gamma Rays in Curcuma alismatifolia

HortScience ◽  
2013 ◽  
Vol 48 (11) ◽  
pp. 1346-1351 ◽  
Author(s):  
Sima Taheri ◽  
Thohirah Lee Abdullah ◽  
Nur Ashikin Psyquay Abdullah ◽  
Zaiton Ahmad
Keyword(s):  
Genetic Variation ◽  
Genetic Similarity ◽  
Dna Polymorphism ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
The Other ◽  
Sequence Repeat ◽  
Induced Mutations ◽  
Similarity Coefficients ◽  
Issr Analysis

In plant breeding programs with vegetatively propagated plants, induced mutations with low physiological effects and strong genetic effects are important approaches for broadening the genetic variation. In the present study, intersimple sequence repeat (ISSR) assays were used to identify DNA polymorphism among the mutant varieties of Curcuma alismatifolia (Chiang Mai Red, Sweet Pink, Kimono Pink) and one hybrid (Doi Tung 554) exposed to 0-, 10-, and 20-Gy optimum doses of acute gamma irradiation. To identify DNA polymorphism among the mutants (10 and 20 Gy) and non-treated (0 Gy) samples through ISSR assay, a total of 25 random primers were screened and 14 gave reproducible polymorphic bands. The number of presumed alleles revealed by the ISSR analysis ranged from three to 19 alleles with mean values of 6.5, 7.8, and 9.2 alleles per locus for doses of 0 (control), 10, and 20 Gy, respectively. The average effective number of alleles, Nei’s gene diversity, Shannon’s information index, and polymorphic information content (PIC) were 1.41 to 1.48, 0.24 to 0.33, 0.35 to 0.45, and 0.21 to 0.25 for the three doses, respectively. Percentages of polymorphic loci for non-irradiated (0 Gy), 10 Gy, and 20 Gy individual plants were 76.4%, 83.7%, and 85.8%, respectively, with an average of 81.9%. Jaccard’s genetic similarity coefficient varied from 0.19 to 1.0 indicating the level of genetic variation among the mutants studied. In terms of variety, the range of genetic variation (0.27 to 0.4) among individuals of the Doi Tung population was more than that observed in the other three varieties. In a dendrogram constructed based on genetic similarity coefficients, the 44 C. alismatifolia individual plants fell into seven major clusters at a coefficient level of 0.35. The study revealed that DNA polymorphism detected by ISSR analysis offered a useful molecular marker for the identification of mutants in gamma radiation-treated plants. Additionally, this research demonstrated that the DNA of the hybrid (Doi Tung 554) showed a greater response in induced mutation compared with the other varieties.

scholarly journals Molecular Distinction Among Mulberry (Morus Spp.) Species and Varieties Cultivated in DPR Korea

2021 ◽  
Author(s):  
Un-Hyang Ho ◽  
Jung Sam Kye ◽  
Song Im Chae ◽  
Jong Ho Kim ◽  
Myong Ho Kim
Keyword(s):  
Genetic Variation ◽  
Internal Transcribed Spacer ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Breeding Strategy ◽  
Issr Analysis ◽  
Morus Spp ◽  
Simple Sequence ◽  
Issr Primers

Abstract Mulberry (Morus spp.) is a cross-pollinating and highly hybridized plant of which productivity are greatly varied in different varieties. We analysed molecular distinction among four mulberry species and varieties cultivated in DPR Korea by using nuclear ribosomal internal transcribed spacer (ITS) sequences and inter simple sequence repeat (ISSR) markers. ITS sequences didn’t represent a remarkable interspecific distinction among four mulberry species used in our study, suggesting that it could not be employed to identify them. ISSR analysis using 16 random primers generated 158 different markers ranging from 100 to 4000 bp in size. The results showed the inter-specific genetic variation (55.34%) was slightly higher than intra-specific genetic variation(44.66%), with comparatively low average number of migrants per generation (Nm) among populations (0.3886). Using ISSR primers selected in this study, in the future, the suitable breeding strategy might be established in raising of elite mulberry varieties on the basis of interspecific hybridization.

scholarly journals Elaeis oleifera (Kunth) Cortés: A neglected palm from the Ecuadorian Amazon

2018 ◽  
Vol 39 (1) ◽  
Author(s):  
Rommel Montúfar ◽  
Claude Louise ◽  
Timothy Tranbarger
Keyword(s):  
Genetic Variation ◽  
Local Level ◽  
Reproductive Barriers ◽  
Reproductive Characteristic ◽  
Isolated Populations ◽  
Tropical Regions ◽  
Biochemical Characteristics ◽  
Elaeis Oleifera ◽  
Ecuadorian Amazon ◽  
Fertile Hybrids

Knowledge of the biology of Ecuadorian palms remains very limited. A particular case is the American oil palm, Elaeis oleifera (Kunth) Cortés, first described in Ecuador in 1986. The genus Elaeis has a trans-Atlantic (Africa-America) distribution, with E. oleifera from the Neotropics and E. guineensis Jacq. from Africa. It has been hypothesized that E. oleifera derives from populations of E. guineensis, which diverged 15 million years ago. At the local level, the populations of E. oleifera have a disjunctive distribution, with isolated populations within the Amazon and Caribbean regions, frequently associated with human or archaeological settlements. Despite the spatial and historical separation between the two species, there are no reproductive barriers to the generation of fertile hybrids. This important reproductive characteristic has allowed E. oleifera to become a major source of genetic variation for the improvement and adaptability of commercial populations of E. guineensis throughout the planet. The Ecuadorian populations of E. oleifera from Taisha - with morphological, reproductive and agronomically important biochemical characteristics -  have been used for the creation of commercial hybrids, which today are planted in tropical regions.

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