scholarly journals Validation of somatic cell score-associated SNPs from Holstein cattle in Sudanese Butana and Butana × Holstein crossbred cattle

2022 ◽  
Vol 54 (1) ◽  
Author(s):  
Salma Elzaki ◽  
Paula Korkuc ◽  
Danny Arends ◽  
Monika Reissmann ◽  
Siham A. Rahmatalla ◽  
...  

AbstractThe Bos indicus zebu cattle Butana is the most commonly used indigenous dairy cattle breed in Sudan. In the last years, high-yielding Holstein dairy cattle were introgressed into Butana cattle to improve their milk yield and simultaneously keep their good adaption to extreme environmental conditions. With the focus on the improvement of milk production, other problems arose such as an increased susceptibility to mastitis. Thus, genetic selection for mastitis resistance should be considered to maintain healthy and productive cows. In this study, we tested 10 single nucleotide polymorphisms (SNPs) which had been associated with somatic cell score (SCS) in Holstein cattle for association with SCS in 37 purebred Butana and 203 Butana × Holstein crossbred cattle from Sudan. Animals were genotyped by competitive allele-specific PCR assays and association analysis was performed using a linear mixed model. All 10 SNPs were segregating in the crossbred Butana × Holstein populations, but only 8 SNPs in Sudanese purebred Butana cattle. The SNP on chromosome 13 was suggestively associated with SCS in the Butana × Holstein crossbred population (rs109441194, 13:79,365,467, PBF = 0.054) and the SNP on chromosome 19 was significantly associated with SCS in both populations (rs41257403, 19:50,027,458, Butana: PBF = 0.003, Butana × Holstein: PBF = 6.2 × 10−16). The minor allele of both SNPs showed an increase in SCS. Therefore, selection against the disadvantageous minor allele could be used for genetic improvement of mastitis resistance in the studied populations. However, investigations in a bigger population and across the whole genome are needed to identify additional genomic loci.

2021 ◽  
Author(s):  
Salma Elzaki ◽  
Paula Korkuc ◽  
Danny Arends ◽  
Monika Reissmann ◽  
Siham A. Rahmatalla ◽  
...  

Abstract The Bos indicus zebu cattle Butana is the most commonly used indigenous dairy cattle breed in Sudan. In the last years, high-yielding Holstein dairy cattle were introgressed into Butana cattle to improve their milk yield and simultaneously keep their good adaption to extreme environmental conditions. With the focus on the improvement of milk production, other problems arose such as an increased susceptibility to mastitis. Thus, genetic selection for mastitis resistance should be considered to maintain healthy and productive cows. In this study, we tested 10 SNPs which had been associated with somatic cell score (SCS) in Holstein cattle for association with SCS in 37 purebred Butana and 203 Butana × Holstein crossbred cattle from Sudan. Animals were genotyped by allele-specific KASP assays and association analysis was performed using a linear mixed model. All 10 SNPs were segregating in the crossbred Butana x Holstein populations, but only 8 SNPs in Sudanese purebred Butana cattle. The SNP on chromosome 13 was suggestively associated with SCS in the Butana x Holstein crossbred population (rs109441194, 13:79,365,467, PBF = 0.054) and the SNP on chromosome 19 was significantly associated with SCS in both populations (rs41257403, 19:50,027,458, Butana: PBF=0.003, Butana x Holstein: PBF=6.2x10-16). The minor allele of both SNPs showed an increasing in SCS. Therefore, selection against the disadvantageous minor allele could be used for genetic improvement of mastitis resistance in the studied populations. However, investigations in a bigger population and across the whole genome are needed to identify additional genomic loci.


Gene Reports ◽  
2018 ◽  
Vol 12 ◽  
pp. 255-260
Author(s):  
Jay Prakash Gupta ◽  
Bharat Bhushan ◽  
V.N. Muhasin Asaf ◽  
Amod Kumar ◽  
Sanjeev Ranjan ◽  
...  

2019 ◽  
Vol 102 (1) ◽  
pp. 452-463 ◽  
Author(s):  
H.R. Oliveira ◽  
L.F. Brito ◽  
F.F. Silva ◽  
D.A.L. Lourenco ◽  
J. Jamrozik ◽  
...  

BMC Genetics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 50 ◽  
Author(s):  
Bodo Brand ◽  
Christine Baes ◽  
Manfred Mayer ◽  
Norbert Reinsch ◽  
Christa Kühn

2017 ◽  
Vol 62 (No. 4) ◽  
pp. 157-167
Author(s):  
N. Chen ◽  
F. Wang ◽  
N. Yu ◽  
Y. Gao ◽  
J. Huang ◽  
...  

The primary agent of mastitis is a wide spectrum of bacterial strains; however, viral-related mastitis has also been reported. The MX dynamin-like GTPase 1 (MX1) gene has been demonstrated to confer positive antiviral responses to many viruses, and may be a suitable candidate gene for the study of disease resistance in dairy cattle. The present study was conducted to investigate the genetic diversity of the MX1 gene in Chinese cattle breeds and its effects on mastitis in Holstein cows. First, polymorphisms were identified in the complete coding region of the bovine MX1 gene in 14 Chinese cattle breeds. An association study was then carried out, utilizing polymorphisms detected in Holstein cows to determine the associations of these single nucleotide polymorphisms (SNPs) with mastitis. We identified 13 previously reported SNPs in Chinese domestic cattle and four of them in Holstein cattle. A novel 12 bp indel was also discovered in Holstein cattle. In addition, haplotype frequencies and linkage disequilibrium of four SNPs detected in Holstein cows were investigated. Analysis of these four SNPs in Chinese Holstein cows revealed two SNPs (g.143181370 T&gt;C and g.143182088 C&gt;T)<br /> significantly (P &lt; 0.05) associated with somatic cell score (SCS). The results indicated that SNPs in the MX1 gene might contribute to the variations observed in the SCS of dairy cattle. Therefore, implementation of these two mutations in selection indexes of the dairy industry might be beneficial by favouring the selection individuals with lower SCS.


2017 ◽  
Vol 95 (suppl_4) ◽  
pp. 82-83 ◽  
Author(s):  
A. A. Sermyagin ◽  
E. A. Gladyr' ◽  
A. A. Kharzhau ◽  
K. V. Plemyashov ◽  
E. N. Tyurenkova ◽  
...  

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