Chronic ciprofloxacin and atrazine co-exposure aggravates locomotor and exploratory deficits in non-target detritivore speckled cockroach (Nauphoeta cinerea)

2021 ◽  
Author(s):  
Isaac A. Adedara ◽  
Umin-Awaji S. Godswill ◽  
Miriam A. Mike ◽  
Blessing A. Afolabi ◽  
Chizoba C. Amorha ◽  
...  
Keyword(s):  
Nauphoeta Cinerea

Glycogen Phosphorylase in the Fat Body of Two Cockroach Species, Periplaneta americana and Nauphoeta cinerea: Isolation, Partial Characterization of Three Forms and Activation by Hypertrehalosaemic Hormones

1991 ◽  
Vol 46 (1-2) ◽  
pp. 149-162 ◽  
Author(s):  
Gerd Gäde
Keyword(s):  
Anion Exchange ◽  
Glycogen Phosphorylase ◽  
Periplaneta Americana ◽  
Fat Body ◽  
Phosphorylase Kinase ◽  
Phosphorylase Activity ◽  
Strong Inhibitor ◽  
Nauphoeta Cinerea ◽  
Crude Extracts ◽  
Fat Bodies

The presence of endogenous phosphorylase kinase and phosphorylase phosphatase in crude extracts of fat bodies from the cockroaches Nauphoeta cinerea and Periplaneta americana is demonstrated in vitro by activation/inactivation of glycogen phosphorylase under appropriate conditions. Fractionation of fat body extracts of both cockroach species on an anion-exchange medium results in the elution of three peaks with phosphorylase activity. According to their AMP dependency these activity peaks are designated as phosphorylase b (inactive without AMP), phosphorylase ab (active without AMP, but several stimulated with AMP) and phosphorylase a (active without AMP). It is shown chromatographically that incubating crude extracts of fat bodies from both cockroaches, under conditions where the phosphorylase kinase is active, results in all phosphorylase b being converted to the ab- or a-form , whereas under conditions where the phosphorylase phosphatase is active all phophorylase a is converted to the ab- or b-form . Endogenous phosphorylase kinase of N. cinerea crude fat body extract can convert vertebrate phosphorylase b into the a-form , and, conversely, vertebrate muscle p hosphorylase kinase and phosphorylase phosphatase, respectively, are able to convert partially purified N. cinerea phosphorylase aborb and the ab- und a-form , respectively. In resting cockroaches most of the phosphorylase activity resides in the b-form and only a small fraction (10% ) in the a-form , whereas between 26% (N . cinerea) and 35% (P. americana) occurs in the ab-form . Injection of endogenous hypertrehalosaemic peptides into N. cinerea (the decapeptide Bld-HrTH ) or P. americana (the two octapeptides Pea-CAH -I and II) causes interconversion of phosphorylase; after injection, mainly (60% ) phosphorylase a is present, while 25% and 15% exists in the ab- und b-form , respectively. Purification of the three phosphorylase forms from N. cinerea is achieved by anion-exchange chromatography on DEAE-Sephacel followed by affinity chromatography on AMP-Sepharose. The final specific activities are 2.1, 6.9 and 27.2 U /mg protein for the a-, ab- und b-form . The molecular mass of the active molecules on gel filtration is between 173,000 and 177,000, and SDS gel electrophoresis reveals a subunit mass of 87,100, suggesting a homodimeric structure for all three form s. Kinetic studies show hyperbolic saturation curves for the substrates glycogen and Pi respectively, with Kᴍ-values of 0.021, 0.019 and 0.073% for glycogen and 8.3, 6.3 and 17.9 mᴍ for Pi (a-, ab- and b-form ). Phosphorylase a exhibits a more or less hyperbolic response to AMP and needs 70 |iM A M P for m axim al stim ulation. The kinetics for the ab- and b-form s are sigm oidal and maximal activities are displayed at about 3 mᴍ (half-maximum activation as calculated from Hill plots are 55 and 280 μᴍ for the ab- und b-form , respectively). Caffeine is a strong inhibitor of the b-form , but has only a slight inhibiting effect (10 -20 % ) on the ab- and a-form in the presence of AMP.

Antenna contact and agonism in the male lobster cockroach, Nauphoeta cinerea

2007 ◽  
Vol 52 (2) ◽  
pp. 252-260 ◽  
Author(s):  
Szu-Ying Chou ◽  
Zachary Y. Huang ◽  
Shu-Chun Chen ◽  
Rou-Ling Yang ◽  
Rong Kou
Keyword(s):  
Nauphoeta Cinerea

C-heterochromatin during synapsis and recombination in grey cockroach Nauphoeta cinerea spermatogenesis

2009 ◽  
Vol 3 (3) ◽  
pp. 289-296
Author(s):  
O. S. Kornienko ◽  
A. M. Gusachenko ◽  
L. V. Vysotskaya
Keyword(s):  
Nauphoeta Cinerea

scholarly journals Mechanism of the Entomotoxic Activity Induced by Araucaria Angustifolia Methanolic Extract in Nauphoeta Cinerea Lobster Cockroaches

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Carrazoni Thiago ◽  
Vieira Patrícia de Brum ◽  
Silva Patrícia Gomes da ◽  
Heberle Marines de Avila ◽  
Sturmer Graziele Daiane ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Araucaria Angustifolia ◽  
Nauphoeta Cinerea

scholarly journals Subsocial Cockroaches Nauphoeta cinerea Mate Indiscriminately with Kin Despite High Costs of Inbreeding

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0162548 ◽  
Author(s):  
Sofia Bouchebti ◽  
Virginie Durier ◽  
Cristian Pasquaretta ◽  
Colette Rivault ◽  
Mathieu Lihoreau
Keyword(s):  
Nauphoeta Cinerea
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