Clinical observations of sequential therapy with Chinese medicine and hysteroscopic mechanical stimulation of the endometrium in infertile patients with repeated implantation failure undergoing frozen-thawed embryo transfer

2014 ◽  
Vol 21 (4) ◽  
pp. 249-253 ◽  
Author(s):  
Xiao-le Zhang ◽  
Yong-lun Fu ◽  
Yan Kang ◽  
Cong Qi ◽  
Qin-hua Zhang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Embryo Transfer ◽  
Mechanical Stimulation ◽  
Sequential Therapy ◽  
Implantation Failure ◽  
Clinical Observations ◽  
Infertile Patients ◽  
Stimulation Of ◽  
Frozen Thawed Embryo Transfer ◽  
Thawed Embryo Transfer

scholarly journals Clinical value of histologic endometrial dating for personalized frozen-thawed embryo transfer in patients with repeated implantation failure in natural cycles

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Yuan Li ◽  
Xiao feng Li ◽  
Jing nan Liao ◽  
Xiang xiu Fan ◽  
Yong bin Hu ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Endometrial Biopsy ◽  
Implantation Failure ◽  
Cumulative Live Birth Rate ◽  
Repeated Implantation Failure ◽  
Link Type ◽  
Infertile Patients ◽  
Frozen Thawed Embryo Transfer ◽  
Natural Cycles ◽  
Thawed Embryo Transfer

Abstract Background Displacement of the window of implantation (WOI) has been proposed as an important factor contributing to repeated implantation failure (RIF). However, the use of histologic endometrial dating as a diagnostic tool of endometrial receptivity has been questioned. Methods This study is a prospective intervention trial that enrolled 205 infertile patients from July 2017 to December 2017. Endometrial biopsies from 50 patients with good prognoses were conducted on day 3 (n = 6), 5 (n = 6), 7 (n = 26), 9 (n = 6), or 11 (n = 6) post-ovulation (PO + 3/5/7/9/11) of the previous natural cycle before their conventional frozen-thawed embryo transfer (FET) cycle. We conducted endometrial biopsies for 155 RIF patients on day PO + 7. Results The verification of the Noyes criteria for endometrial dating was conducted at different times (PO + 3/+ 5/+ 7/+ 9/+ 11) on 41 patients with good prognoses who achieved an ongoing pregnancy in their first conventional FET cycle after endometrial biopsy. The agreement between two pathologists determining endometrial biopsy dating results in infertile patients was determined to be acceptable (weighted kappa = 0.672, P < 0.001). The rate of out-of-phase dating on day PO + 7 was significantly higher in RIF patients than in good- prognosis patients (31.6% vs. 3.8%, P = 0.003). pFET was performed in 47 RIF patients diagnosed to be out of phase, and the cumulative live-birth rate was 61.7%. Conclusions Histologic endometrial dating of RIF patients in natural cycles may be a biomarker for a receptive endometrium in diagnosing WOI displacement. Trial registration NCT03312309 Registered 17 October 2017. NCT03222830 Registered 19 July 2017,

THE EFFECT OF ASSISTED HATCHING IN FROZEN-THAWED EMBRYO TRANSFER CYCLES

2016 ◽  
pp. 80-84
Author(s):  
Thi Tam An Nguyen ◽  
Minh Tam Le ◽  
Ngoc Thanh Cao
Keyword(s):  
Embryo Transfer ◽  
Zona Pellucida ◽  
Assisted Hatching ◽  
Implantation Failure ◽  
Multiple Pregnancies ◽  
Recurrent Implantation Failure ◽  
Key Words Laser ◽  
Implantation And Pregnancy Rates ◽  
Frozen Thawed Embryo Transfer ◽  
Thawed Embryo Transfer

Background: Laser assisted hatching technique based on the hypothesis to make an artificial hole on zona pellucida (ZP) that can help embryo hatching out of ZP easily. This technique has been shown to increase implantation and pregnancy rates in women of advanced age, in women with recurrent implantation failure and following the transfer of frozen–thawed embryos. This study described the outcome of frozen–thawed embryo transfers with laser assisted hatching (LAH), which is one of the safest method in nowadays. Purpose: To assess the effect of assisted hatching technique on the clinical outcomes in vitrified-warmed transfer cycles. Method: A total of 65 thawed-transfer cycles with 153 thawed-embryos undertaken within a 12-month period were analysed, Assisted hatching with laser zona thinning was performed with one-quarter of the zona pellucida circumference. The overall thawed-embryos (day 3) were kept in culture overnight. Patient were prepared the suitable endometrium and transferred embryos advantageously. Results: In which, having the rate of survival embryos were 143 occupying 94.3%, the percentage of grade 1 and 2 embryos occupied 55.9% and 29,3% respectively, and that were enrolled LAH before transfering of frozen–thawed embryos. The average transferred embryos were 2.4±0.8, The rate of implantation per transferred embryos and per transferred embryos cycles was 19.5% and 43.1% respectively. The rate of clinical pregnancies per embryo transfer cycles occupied 33.8% with percentage of early miscarriages (biochemical pregnancies and early clinical miscarriages) was 12.3%. The rate of ongoing pregnancies was 30.8% and multiple pregnancies was low just 12.3%. This result was equal or higher than other researchs in embryos transfer had or no LAH. Conclusion: LAH contributed to stable frozen–thawed embryos transfer effectiveness. Key words: Laser assisted hatching, frozen–thawed embryos transfer, zona pellucida (ZP)

scholarly journals Clinical value of histologic dating of the endometrium for personalized frozen-thawed embryo transfer (pFET) in patients with repeated implantation failure (RIF) in natural cycles

2020 ◽  
Author(s):  
Yuan Li ◽  
Xiaofeng Li ◽  
Jingnan liao ◽  
Xiangxiu Fan ◽  
Yongbin Hu ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Live Birth Rate ◽  
Good Prognosis ◽  
Endometrial Biopsy ◽  
Clinical Value ◽  
Cumulative Live Birth Rate ◽  
Infertile Patients ◽  
Frozen Thawed Embryo Transfer ◽  
Natural Cycles ◽  
Thawed Embryo Transfer

Abstract Background: Displacement of the window of implantation (WOI) has been proposed as an important factor contributing to RIF. However, histologic dating of the endometrium as a diagnostic tool of endometrial receptivity has been questioned. Methods: This is a prospective intervention trial that entailed 205 infertile patients from July 2017 to December 2017. Endometrial biopsies from 50 good-prognosis patients were conducted on day 3 (n=6), 5 (n=6), 7 (n=26), 9 (n=6) or 11 (n=6) post-ovulation (PO+3/5/7/9/11) of the previous natural cycle before their conventional frozen-thawed embryo transfer (FET) cycle. We conducted endometrial biopsies of 155 RIF patients on day PO +7. Results: The verification of the Noyes criteria for endometrial dating was conducted at different times (PO +3/+5/+7/+9/+11) on 41 good-prognosis patients who achieved an ongoing pregnancy in their first conventional FET cycle after endometrial biopsy. The agreement between two pathologists for endometrial biopsy dating in infertile patients was determined to be acceptable (weighted kappa = 0.672, P < 0.001). The rate of out-of-phase dating on day PO+7 was significantly higher in RIF patients than good- prognosis patients (31.6% vs . 3.8%, P=0.003). pFET was performed in 47 RIF patients diagnosed to be out of phase, and the cumulative live-birth rate was 61.7%. Conclusions: Histologic endometrial dating of RIF patients in natural cycles may be a biomarker for a receptive endometrium in diagnosing the displacement of WOI.

scholarly journals Effect of Intrauterine Administration of Seminal Plasma for Patients with Recurrent Implantation Failure Before FET: Study Protocol for a Prospective Randomised Controlled Trial

2021 ◽  
Author(s):  
ling CUI ◽  
Fang Wang ◽  
Yonghong Lin
Keyword(s):  
Embryo Transfer ◽  
Study Protocol ◽  
Seminal Plasma ◽  
Controlled Trial ◽  
Clinical Pregnancy ◽  
Implantation Failure ◽  
Central Hospital ◽  
Recurrent Implantation Failure ◽  
Frozen Thawed Embryo Transfer ◽  
Thawed Embryo Transfer

Abstract Background: To assess the effect of intrauterine administration of seminal plasma for patients with recurrent implantation failure before frozen-thawed embryo transfer. Methods: Trial design: This is a parallel group, randomized (1:1 allocation ratio) controlled trial.Participants: All patients will be recruited from Chengdu Women’s and Children’s Central Hospital. Inclusion criteria: 1. Women after IVF/ICSI treatment in Chengdu Women’s and Children’s Central Hospital. 2.Infertile women with a history of recurrent implantation failure. 3.Infertile couples (both male and female) aged between 20 and 39 years;4. Couples who have at least 1 good quality embryos for transfer. 5. Males had negative in infectious disease test. 6. The males have semen. 7. Women who intend to undergo FET after IVF or ICSI or pre-implantation genetic testing for aneuploidy (PGT-A). 8. Competent and able to give informed consent. Intervention and comparator: Treatment group receiving intrauterine administration of seminal plasma before frozen-thawed embryo transfer. Main outcomes: Clinical pregnancy after frozen-thawed embryo transfer. Randomisation: Patients will be randomly allocated to either treatment or control group at 1:1 ratio. Random numbers will be generated by using software SPSS 25.0 performed by an independent statistician from Chengdu Women’s and Children’s Central Hospital. Blinding (masking): Only the data analyst will be blinded to group assignment. Numbers to be randomised (sample size): To account for a 10% dropout rate, we will recruit 174 patients (87 in each group). Trial status: The date of approval is 31rd May 2021, version 2.0. Recruitment started on 1rd June and is expected to end in July 2022. Discussion: Intrauterine administration of seminal plasma before frozen-thawed embryo transfer of patients with recurrent implantation failure may improve clinical pregnancy rate, it has great Page 2 of 14 significance for assisted reproduction. Trial registration: The study protocol has been approved by the ethics committees at Chengdu Women’s and Children’s Central Hospital. The trial was registered at the Chinese Clinical Trial Registry ChiCTR2100046803. Registered on 28 May 2021.

scholarly journals Clinical value of endometrial histological dating for personalized frozen-thawed embryo transfer (pFET) in patients with repeated implantation failure (RIF) in natural cycle

2019 ◽  
Author(s):  
Yuan Li ◽  
Xiaofeng Li ◽  
Jingnan liao ◽  
Xiangxiu Fan ◽  
Yongbin Hu ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Birth Rate ◽  
Live Birth Rate ◽  
Weighted Kappa ◽  
Good Prognosis ◽  
Natural Cycle ◽  
Clinical Value ◽  
Infertile Patients ◽  
Frozen Thawed Embryo Transfer ◽  
Thawed Embryo Transfer

Abstract Background: The displacement of window of implantation (WOI) has been proposed as an important factor contributed to RIF. However, endometrial histological dating as the diagnostic tool of endometrial receptivity has been questioned. Methods : This is a prospective intervention trial including 205 infertile patients from July 2017 to December 2017.Endometrial biopsies from 50 good prognosis patients were conducted on day 3(n=6), 5(n=6), 7(n=26), 9(n=6) or 11(n=6) of post-ovulation (PO+3/5/7/9/11) in the previous natural cycle before their conventional frozen-thawed embryo transfer (FET) cycle. The endometrial biopsies of 155 RIF patients were conducted on day of PO +7. Results: The verification of Noyes criterion for endometrial dating was conducted in different time (PO +3/+5/+7/+9/+11) from 41 good prognosis patients achieving ongoing pregnancy in the first conventional FET cycle after the endometrial biopsies. The agreement between two pathologists for endometrium dating in infertile patients was determined to be good (weighted kappa = 0.672; P < 0.001). The rate of out-of-phase dating on the day of PO+7 was significantly higher in RIF patients than good prognosis patients (31.6% vs 3.8%, P=0.003).pFET was performed in 47 RIF patients diagnosed to be out of phase, and the accumulative live birth rate was 55.7%. Conclusions: The endometrial histological dating in RIF patients in natural cycle may be a endometrial receptive biomarker for diagnosing the displacement of WOI.

scholarly journals Clinical value of histologic dating of the endometrium for personalized frozen-thawed embryo transfer (pFET) in patients with repeated implantation failure (RIF) in natural cycles

2019 ◽  
Author(s):  
Yuan Li ◽  
Xiaofeng Li ◽  
Jingnan liao ◽  
Xiangxiu Fan ◽  
Yongbin Hu ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Live Birth Rate ◽  
Good Prognosis ◽  
Endometrial Biopsy ◽  
Clinical Value ◽  
Cumulative Live Birth Rate ◽  
Infertile Patients ◽  
Frozen Thawed Embryo Transfer ◽  
Natural Cycles ◽  
Thawed Embryo Transfer

Abstract Background: Displacement of the window of implantation (WOI) has been proposed as an important factor contributing to RIF. However, histologic dating of the endometrium as a diagnostic tool of endometrial receptivity has been questioned. Methods: This is a prospective intervention trial that entailed 205 infertile patients from July 2017 to December 2017. Endometrial biopsies from 50 good-prognosis patients were conducted on day 3 (n=6), 5 (n=6), 7 (n=26), 9 (n=6) or 11 (n=6) post-ovulation (PO+3/5/7/9/11) of the previous natural cycle before their conventional frozen-thawed embryo transfer (FET) cycle. We conducted endometrial biopsies of 155 RIF patients on day PO +7. Results: The verification of the Noyes criteria for endometrial dating was conducted at different times (PO +3/+5/+7/+9/+11) on 41 good-prognosis patients who achieved an ongoing pregnancy in their first conventional FET cycle after endometrial biopsy. The agreement between two pathologists for endometrial biopsy dating in infertile patients was determined to be acceptable (weighted kappa = 0.672, P < 0.001). The rate of out-of-phase dating on day PO+7 was significantly higher in RIF patients than good- prognosis patients (31.6% vs . 3.8%, P=0.003). pFET was performed in 47 RIF patients diagnosed to be out of phase, and the cumulative live-birth rate was 55.7%. Conclusions: Histologic endometrial dating of RIF patients in natural cycles may be a biomarker for a receptive endometrium in diagnosing the displacement of WOI.

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