Molecular characterization of the chitinase genes of native Bacillus thuringiensis isolates and their antagonistic activity against three important phytopathogenic fungi

Biologia ◽  
2021 ◽  
Author(s):  
Zehra Busra Azizoglu ◽  
Semih Yilmaz ◽  
Ugur Azizoglu ◽  
Salih Karabörklü ◽  
Rıdvan Temizgul ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Molecular Characterization ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Chitinase Genes

Purification and characterization of an exochitinase from Bacillus thuringiensis subsp. aizawai and its action against phytopathogenic fungi

2006 ◽  
Vol 52 (7) ◽  
pp. 651-657 ◽  
Author(s):  
Luis Morales de la Vega ◽  
J Eleazar Barboza-Corona ◽  
Maria G Aguilar-Uscanga ◽  
Mario Ramírez-Lepe
Keyword(s):  
Bacillus Thuringiensis ◽  
Sodium Dodecyl ◽  
Sclerotium Rolfsii ◽  
Phytopathogenic Fungi ◽  
Purification And Characterization ◽  
Chitinolytic Enzyme ◽  
Bacillus Thuringiensis Subsp ◽  
Sds Page ◽  
Fusarium Sp

A chitinolytic enzyme from Bacillus thuringiensis subsp. aizawai has been purified and its molecular mass was estimated ca. 66 kDa by sodium dodecyl sulfate – polyacryamide gel electrophoresis (SDS–PAGE). The enzyme was able to hydrolyze chitin to chitobiosides but not carboxymethylcellulose, cellulose, pullulan, and laminarin. Optimal pH and temperature were detected at 6 and 50 °C, respectively. Stability, in the absence of substrate, was observed at temperatures less than 60 °C and pH between 5 and 8. Enzyme activity was significantly inhibited by K+ and EDTA and completely inhibited by Hg2+. Purified chitinase showed lytic activity against cell walls from six phytopathogenic fungi and inhibited the mycelial growth of both Fusarium sp. and Sclerotium rolfsii. The biocontrol efficacy of the enzyme was tested in the protection of bean seeds infested with six phytopathogenic fungi.Key words: chitinase, Bacillus thuringiensis, purification, phytopathogenic fungi.

Molecular characterization of an endochitinase from Bacillus thuringiensis subsp. konkukian

2010 ◽  
Vol 26 (12) ◽  
pp. 2171-2178 ◽  
Author(s):  
Muhammad Aamer Mehmood ◽  
Xiang Xiao ◽  
Fauzia Yusuf Hafeez ◽  
Yingbao Gai ◽  
Fengping Wang
Keyword(s):  
Bacillus Thuringiensis ◽  
Molecular Characterization ◽  
Bacillus Thuringiensis Subsp

scholarly journals Isolation, morphological and molecular characterization of Bacillus thuringiensis strains against Hypothenemus hampei Ferrari (Coleoptera: Curculionidae: Scolytinae)

2018 ◽  
Vol 62 (3) ◽  
pp. 198-204 ◽  
Author(s):  
Janaina Zorzetti ◽  
Ana Paula Scaramal Ricietto ◽  
Fernanda Aparecida Pires Fazion ◽  
Ana Maria Meneghin ◽  
Pedro Manuel Oliveira Janeiro Neves ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Molecular Characterization ◽  
Hypothenemus Hampei ◽  
Morphological And Molecular Characterization
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