Wheat Bran Hydrolysate Culture Medium Design for Talaromyces purpureogenus CFRM02 Pigment Production and Colour Characteristics

Wheat Bran Hydrolysate Culture Medium Design for Talaromyces Purpureogenus CFRM02 Pigment Production and Colour Characteristics

10.21203/rs.3.rs-840095/v1 ◽

2021 ◽

Author(s):

Sujit Das ◽

Saritha Gopal Pandit ◽

Mohan Dhale

Keyword(s):

Wheat Bran ◽

Carbon Sources ◽

Nitrogen Sources ◽

Pigment Production ◽

Xylose Metabolism ◽

Carbon And Nitrogen ◽

Medium Design ◽

The Media ◽

Comparative Results ◽

Cie Lab

Abstract Wheat bran hydrolysate (WBH) in combination with carbon and nitrogen was utilised as substrate for pigment production by Talaromyces purpureogenus CFRM02. Pigment yield was significantly increased (≈ 3 fold: OD units and ≈ 2 fold: a* value) by xylose supplementation with WBH compared to other carbon sources. Whereas 1% xylose supplementation increased pigment production (1.57 ± 0.05 OD Units and 49 ± 1.62 a* value). Pigment yield was low when WBH supplemented with 0.3% nitrogen sources. However significant increase (≈ 2-2.5 fold, OD units and a* value) was observed, when yeast extract (1.2%), nitrate of sodium (1.2%) and potassium (1.6%) were supplemented. Accordingly, 16 WBH media were designed by supplementing carbon and nitrogen. Interestingly the pigment production was significantly increased (1.59 OD units and 32 a* value) in the medium supplemented with 4% carbon and 0.9–1.2% nitrogen. T. purpureogenus CFRM02 was able to co-utilize xylose, fructose and glucose in WBH medium. The CIE Lab values indicated that, pigment characteristics differed significantly among the media. Apparently, T. purpureogenus CFRM02 possess alternative gene(s) or pathway(s) for xylose metabolism and channelled towards pigment biosynthesis. Comparative results revealed that, 1% xylose supplementation to WBH makes the fermentation process economically competitive for pigment production.

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A New Way of Biological Treatment of Oil Residue - A Case of the Mushroom Cultivation

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.183-185.411 ◽

2011 ◽

Vol 183-185 ◽

pp. 411-416

Author(s):

Xi Qin Wang ◽

Zheng Wen Huang ◽

Bo Yu ◽

Jian Qiang Zhang

Keyword(s):

Wheat Bran ◽

Culture Medium ◽

Raw Materials ◽

Test Group ◽

Raw Material ◽

Control Group ◽

Mushroom Cultivation ◽

Edible Fungi ◽

Oil Residue ◽

Cottonseed Hull

As an industrial organic solid waste, oil residue, with wide sources and low price, but could not find an effective way to use currently. In addition, with the development of mushroom industry and rising price of traditional raw-materials, it is very necessary to find the substitute raw material to reduce production cost of mushroom cultivation. This study attempts to produce the strains of Flammulina velutipes by taking use of oil residue to replace part of the traditional raw material, there are two groups in the experiment: the test group is adding 5%, 10%, 15%, 20%, 40% of the oil residue to replace the culture medium of cottonseed hull and wheat bran; the control group is the traditional culture medium, then comparatively observed the growth of the mycelium. The results showed that it can promote the growth of the mycelium and shorten the cycle of seed production to add the oil residue in the test. But the growth rate of mycelium will be hindered when the concentration exceeds a certain range.The optimal culture medium to add the oil residue is as follows: 15% of the oil residue, 64% cottonseed hull, 20% coarse wood chips, 0% wheat bran , 1% lime. Oil residue contains a lot of the nutritional components to benefit the growth of edible fungi, and without the heavy metal pollution, can replace the culture medium of cottonseed hull and wheat bran and other traditional materials, reduce cost of production the strains, a fair-sized economic efficiency can be received, the environment can be protected.

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PROSPECÇÃO E AVALIAÇÃO DO POTENCIAL BIOTECNOLÓGICO DE LINHAGENS COMERCIAIS DE SHIITAKE

ENERGIA NA AGRICULTURA ◽

10.17224/energagric.2020v35n4p640-649 ◽

2021 ◽

Vol 35 (4) ◽

pp. 640-649

Author(s):

Dalvan Pereira Abilio ◽

Olívia Gomes Martins ◽

Giovana Silva ◽

Meire Andrade

Keyword(s):

Wheat Bran ◽

Radial Growth ◽

Mycelial Growth ◽

Culture Medium ◽

Lentinula Edodes ◽

Culture Media ◽

Biotechnological Potential ◽

E Mail ◽

Eucalyptus Sawdust

PROSPECÇÃO E AVALIAÇÃO DO POTENCIAL BIOTECNOLÓGICO DE LINHAGENS COMERCIAIS DE SHIITAKE DALVAN PEREIRA ABÍLIO1, OLÍVIA GOMES MARTINS2, GIOVANA CRISTINA PINTO ALVES DA SILVA3, MEIRE CRISTINA NOGUEIRA DE ANDRADE4 1 Graduando em Ciências Biológicas, Centro Universitário Sagrado Coração – Unisagrado, R. Irmã Arminda, 10-50 - Jardim Brasil, 17011-160 Bauru, SP, Brasil. E-mail: [email protected] 2 Doutoranda em Agronomia – Energia na Agricultura, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista "Julio de Mesquita Filho" (UNESP), Av. Universitária, 3780 - Altos do Paraíso, 18610-034, Botucatu, SP, Brasil. E-mail: [email protected] 3 Mestre em Agronomia – Energia na Agricultura, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista "Julio de Mesquita Filho" (UNESP), Av. Universitária, 3780 - Altos do Paraíso, 18610-034, Botucatu, SP, Brasil. E-mail: [email protected] 4 Docente da Faculdade Gran Tietê, Av. 15 de Novembro, 125 - Centro, 17340-000, Barra Bonita, SP, Brasil. E-mail: [email protected] RESUMO: O objetivo deste trabalho foi avaliar e comparar o crescimento micelial in vitro de quatro linhagens comerciais de Lentinula edodes (LE-241, LE-242, LE-243 e LE-244) em meio de cultura à base de serragem de eucalipto, suplementado com bagaço de malte ou farelo de trigo. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 2x4 (substratos x linhgens), totalizando oito tratamentos, cada um com cinco repetições. Os substratos foram preparados com 20% de suplementação, acrescidos de calcário (tamponante), umidificados e esterilizados. Os meios de cultura foram preparados a partir da infusão dos substratos, filtração, adição de ágar e esterilização. As placas foram inoculadas com as linhagens e o crescimento radial do micélio na superfície do meio de cultura foi mensurado com paquímetro. O menor desenvolvimento micelial ocorreu com a linhagem LE-241 e com a linhagem LE-243 no substrato suplementado com farelo de trigo. Os maiores desenvolvimentos foram obtidos com as linhagens LE-242 e LE-244 no substrado suplementado com bagaço de malte. O desenvolvimento de todas as linhagens foi maior no substrato suplementado com bagaço de malte. Sendo assim, a utilização do bagaço de malte para o crescimento micelial é uma alternativa viável à suplementação tradicional com farelo de trigo. Palavras-chave: fungos, cogumelo, Lentinula edodes, micélio, bagaço de malte. PROSPECTING AND EVALUATING THE BIOTECHNOLOGICAL POTENTIAL OF SHIITAKE COMMERCIAL STRAINS ABSTRACT: This study aimed to evaluate and compare the in vitro mycelial growth of four commercial Lentinula edodes strains (LE-241, LE-242, LE-243 and LE-244) in a culture medium based on eucalyptus sawdust, supplemented with malt bagasse or wheat bran. The experimental design was completely randomized, in a 2x4 factorial scheme (substrates x strains), totaling eight treatments, each with five repetitions. The substrates were prepared with 20% supplementation, added with lime (buffer), humidified and sterilized. The culture media were prepared from the infusion of substrates, filtration, addition of agar and sterilization. The plates were inoculated with the strains and the radial growth of the mycelium on the surface of the culture medium was measured using a caliper. The smallest mycelial growth occurred with the LE-241 strain and with the LE-243 strain on the substrate supplemented with wheat bran. The greatest growths were obtained with the LE-242 and LE-244 strains in the substrate supplemented with malt bagasse. The development of all strains was greater in the substrate supplemented with malt bagasse. Therefore, the use of malt bagasse for mycelial growth is a viable alternative to traditional wheat bran supplementation. Keywords: fungi, mushroom, Lentinula edodes, mycelium, malt bagasse.

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Effect of high temperature on the growth, sporulation, and pigment production of Botryodiplodia theobromae

Canadian Journal of Botany ◽

10.1139/b74-342 ◽

1974 ◽

Vol 52 (12) ◽

pp. 2631-2634 ◽

Cited By ~ 3

Author(s):

Agnes E. Uduebo

Keyword(s):

High Temperature ◽

Vegetative Growth ◽

Culture Medium ◽

Inhibitory Effect ◽

Pigment Production ◽

Botryodiplodia Theobromae ◽

Acid Media ◽

Pink Pigment

The effect of a high temperature on the growth, sporulation, and pigment production of Botryodiplodia theobromae Pat. was investigated. At 35 °C vegetative growth was diminished, there was no sporulation, and a pink pigment was produced. The pigment was released into the culture medium. The inhibitory effect of the high temperature on sporulation was considered to be partially owing to the reduced vegetative growth. Other possibilities are discussed. The pink pigment was preferentially produced in acid media. It inhibited neither growth nor sporulation under the usual conditions.

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Culture medium for amylase production by toxigenic fungi

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132000000500003 ◽

2000 ◽

Vol 43 (5) ◽

pp. 461-467 ◽

Cited By ~ 11

Author(s):

Edson Luiz Zangrando Figueira ◽

Elisa Yoko Hirooka

Keyword(s):

Aspergillus Flavus ◽

Wheat Bran ◽

Fusarium Moniliforme ◽

Mycelial Growth ◽

Culture Medium ◽

Supernatant Fraction ◽

Production Medium ◽

Toxigenic Fungi ◽

Amylase Production ◽

Ground Corn

Mycelial growth and amylase production by a mycotoxigenic strain of Fusarium moniliforme and Aspergillus flavus were evaluated in a culture medium containing starch, glycerol, wheat bran or corn. With emphasis on corn, different fractions composed by germ, degermed seed, starch, milky stage corn and the respective starch or supernatant fraction were analyzed for F. moniliforme growth . The medium composed of milky stage corn supernatant promoted the best mycelial growth (p<0.05), and it was used to prepare amylase production medium in the next step. The medium composed with 2% ground corn in milky stage corn supernatant (350g of milky stage corn blended with 250mL water and centrifuged) promoted the highest amylase production, which was at the 10th day of fermentation, both for F. moniliforme (42.32U/mL) and A. flavus (4,745.54U/mL).

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Biosynthesis and Functions of a Melanoid Pigment Produced by Species of the Sporothrix Complex in the Presence of l-Tyrosine

Applied and Environmental Microbiology ◽

10.1128/aem.02414-12 ◽

2012 ◽

Vol 78 (24) ◽

pp. 8623-8630 ◽

Cited By ~ 39

Author(s):

Rodrigo Almeida-Paes ◽

Susana Frases ◽

Glauber de Sousa Araújo ◽

Manoel Marques Evangelista de Oliveira ◽

Gary J. Gerfen ◽

...

Keyword(s):

Culture Medium ◽

Uv Light ◽

Sporothrix Schenckii ◽

Pigment Production ◽

Yeast Cells ◽

Fungal Cell ◽

Content Type ◽

Mutant Strains ◽

Important Virulence Factor ◽

Medical Interest

ABSTRACTSporothrix schenckiiis the etiological agent of sporotrichosis, the main subcutaneous mycosis in Latin America. Melanin is an important virulence factor ofS. schenckii, which produces dihydroxynaphthalene melanin (DHN-melanin) in conidia and yeast cells. Additionally,l-dihydroxyphenylalanine (l-DOPA) can be used to enhance melanin production on these structures as well as on hyphae. Some fungi are able to synthesize another type of melanoid pigment, called pyomelanin, as a result of tyrosine catabolism. Since there is no information about tyrosine catabolism inSporothrixspp., we cultured 73 strains, including representatives of newly describedSporothrixspecies of medical interest, such asS. brasiliensis,S. schenckii, andS. globosa, in minimal medium with tyrosine. All strains but one were able to produce a melanoid pigment with a negative charge in this culture medium after 9 days of incubation. AnS. schenckiiDHN-melanin mutant strain also produced pigment in the presence of tyrosine. Further analysis showed that pigment production occurs in both the filamentous and yeast phases, and pigment accumulates in supernatants during stationary-phase growth. Notably, sulcotrione inhibits pigment production. Melanin ghosts of wild-type and DHN mutant strains obtained when the fungus was cultured with tyrosine were similar to melanin ghosts yielded in the absence of the precursor, indicating that this melanin does not polymerize on the fungal cell wall. However, pyomelanin-producing fungal cells were more resistant to nitrogen-derived oxidants and to UV light. In conclusion, at least three species of theSporothrixcomplex are able to produce pyomelanin in the presence of tyrosine, and this pigment might be involved in virulence.

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Statistical optimization of culture medium for yellow pigment production by Thermomyces sp.

Journal of Applied and Natural Science ◽

10.31018/jans.v7i1.590 ◽

2015 ◽

Vol 7 (1) ◽

pp. 203-210

Author(s):

R. Poorniammal ◽

S. Gunasekaran ◽

R. Murugesan

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Yeast Extract ◽

Culture Medium ◽

Yellow Pigment ◽

Pigment Production ◽

Quadratic Model ◽

High Yield ◽

Dipotassium Hydrogen Phosphate ◽

Yellow Pigments

In present study, Thermomyces sp. were able to produce high yield of yellow pigments screened. Pigment production by Thermomyces sp was optimized by employing factorial design and response surface techniques in submerged fermentation. The variables evaluated were the concentrations of, sucrose, yeast extract, ammonium sulphate, magnesium sulphate and dipotassium hydrogen phosphate having as response pigment production. One factor at-a-time method was employed for the optimization of media components. Response surface methodology (RSM) optimized these nutrient parameters for maximum yellow pigment production (1387 OD units), which resulted at 35.5 g/L sucrose 5.5 g/L yeast extract, 2.5 g/L NH4SO4, 0.3 g/L MgSO4 and 1.0 g/L K2HPO4 in the medium. Response surface methodology (RSM) was further used to determine the optimum values of process variables for maximum yellow pigment production. The fit of the quadratic model was found to be significant. A significant increase in yellow pigment production was achieved using RSM.

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The Optimization Study on Solid Culture Medium of North Cordyceps

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.781-784.884 ◽

2013 ◽

Vol 781-784 ◽

pp. 884-888

Author(s):

Xiu Li Qin ◽

De Ming Han

Keyword(s):

Growth Rate ◽

Nutrient Solution ◽

Wheat Bran ◽

Culture Medium ◽

Orthogonal Test ◽

Cordyceps Sinensis ◽

Solid Matrix ◽

Solid Culture ◽

The North ◽

Solid Culture Medium

Artificial cultivation of solid culture medium of North Cordyceps was optimized in this experiment. The results show that, the North Cordyceps can be cultivated in many types of solid culture medium, the growth rate and density of the hyphae was highest in a single solid culture medium with rice as the solid substrate, but adding 10%-15% wheat bran in rice solid culture medium, the growth rate and density of the hyphae is better than the single matrix of rice; the suitable nutrient solution was determined by contrast test and L9 (34) orthogonal test, the composition was sucrose 2%, yeast extract 1.5%, MgSO40.1%, KH2PO4 0.1%; the suitable solid culture medium of the North Cordyceps sinensis was determined by the L9 (33) orthogonal test, the composition was rice 85%, wheat bran 15%, pH 5.5-6.5, the ratio of solid and water ( solid matrix / nutrient solution )was 1:1.5.

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Cellulase Production by Submerged Fermentation Using Biological Materials of Corncob Residue with Aspergillus Niger FC-1

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.648.116 ◽

2013 ◽

Vol 648 ◽

pp. 116-119 ◽

Cited By ~ 2

Author(s):

Sheng Yao Jiang ◽

Hui Qin Shi ◽

Ming Fu Gao ◽

Yun Pan Liu ◽

Xiao Min Fang ◽

...

Keyword(s):

Aspergillus Niger ◽

Carbon Source ◽

Wheat Bran ◽

Culture Medium ◽

Fermentation Broth ◽

Cellulase Activity ◽

Cellulase Production ◽

Medium Components ◽

Shake Flasks ◽

Optimal Medium

In order to improve the cellulase-producing ability, the culture medium components and condition were optimized. The results showed that the cellulase activity in the fermentation broth remarkably increased by using 1% corncob residue combined with 1% wheat bran and 1% corncob as carbon source, taking 0.75% soybean meal and 0.3% KON3 as nitrogen source, and adding 0.3% CaCO3 to stabilize the pH to 5.0. The FPase activity in supernatant of A. niger FC-1 fermented with 50mL of the optimal medium in 250mL shake flasks at 30°C reached 101.6U/mL, which was 4.7 times of that before optimization.

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